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- 저자 : Ki Seab Kim (검색결과 4 건)
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Comparison of different ploidy detection methods in Oncorhynchus mykiss, the rainbow trout
Kim, Hong Seab,Chung, Ki-Hwa,Son, Jung-Ho The Korean Society of Fisheries and Aquatic Scienc 2017 Fisheries and Aquatic Sciences Vol.20 No.11
The objective of this study was to determine a simple and reliable ploidy identification protocol for the rainbow trout (RT), Oncorhynchus mykiss, in the field condition. To evaluate the ploidy level and compare different detection protocols, triploid RT and gynogenesis were induced by UV irradiation and/or heat shock. The hatching rate at day 30 was 85.2% and the survival rate at day 90 was 69.4% (fingerling). The sex ratio of female RT was 93.75% in the gynogenesis group, illustrating that the UV irradiation inactivated the sperm DNA. The hatching rate and survival rate were 82.0 and 74.7%, respectively, in the triploid-induced group. The triploid induction rate by heat shock procedure was 73.9%. Cytogenetic protocols for ploidy identification such as chromosome counting, erythrocyte nuclear size comparison, and analysis of nucleolar organizing regions (NORs) by silver staining were compared. Silver nitrate staining showed the greatest success rate (22/23 and 32/32 for the triploid-induced group and gynogenesis group, respectively), followed by erythrocyte nuclear size comparison (16/23 and 19/32 for the triploid-induced group and gynogenesis group, respectively) and, lastly, chromosome preparation (2/23 and 6/32 for the triploid-induced group and gynogenesis group, respectively) with the lowest success rate. Based on our findings, silver staining for RT ploidy identification is speculated to be highly applicable in a wide range of research conditions, due to its cost-effectiveness and simplicity compared to other numerous ploidy detection protocols.
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Comparison of different ploidy detection methods in Oncorhynchus mykiss, the rainbow trout
( Hong Seab Kim ),( Ki-hwa Chung ),( Jung-ho Son ) 한국수산과학회(구 한국수산학회) 2017 Fisheries and Aquatic Sciences Vol.20 No.4
The objective of this study was to determine a simple and reliable ploidy identification protocol for the rainbow trout (RT), Oncorhynchus mykiss, in the field condition. To evaluate the ploidy level and compare different detection protocols, triploid RT and gynogenesis were induced by UV irradiation and/or heat shock. The hatching rate at day 30 was 85.2% and the survival rate at day 90 was 69.4% (fingerling). The sex ratio of female RT was 93.75% in the gynogenesis group, illustrating that the UV irradiation inactivated the sperm DNA. The hatching rate and survival rate were 82.0 and 74.7%, respectively, in the triploid-induced group. The triploid induction rate by heat shock procedure was 73.9%. Cytogenetic protocols for ploidy identification such as chromosome counting, erythrocyte nuclear size comparison, and analysis of nucleolar organizing regions (NORs) by silver staining were compared. Silver nitrate staining showed the greatest success rate (22/23 and 32/32 for the triploid-induced group and gynogenesis group, respectively), followed by erythrocyte nuclear size comparison (16/23 and 19/32 for the triploid-induced group and gynogenesis group, respectively) and, lastly, chromosome preparation (2/23 and 6/32 for the triploid-induced group and gynogenesis group, respectively) with the lowest success rate. Based on our findings, silver staining for RT ploidy identification is speculated to be highly applicable in a wide range of research conditions, due to its cost-effectiveness and simplicity compared to other numerous ploidy detection protocols.
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송병섭(Byeang-Seab Song),엄기명(Ki-Myeong Eom),윤용호(Yong-Ho Yoon),원충연(Chung-Yuen Won),김덕근(Duk-Gun Kim) 한국조명·전기설비학회 2000 한국조명·전기설비학회 학술대회논문집 Vol.2000 No.-
Switched reluctance motor drives have been finding their applications in the variable speed drives due to their relatively low cost, simple and robust structure, controllability and high efficiency. Fuzzy control does not need any model of plant. It is based on plant operator experience and heuristics. Fuzzy control is basically adaptive and gives robust performance for plant parameter variation. This paper deals with the speed control of switched reluctance motor using fuzzy controller with 7-rule based fuzzy logic. The proposed fuzzy controller is superior to the control performance of the conventional PI controller. The fuzzy controller is implemented by 80C196KC, 16 bit one-chip microcontroller.
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김동국,김기섭,장영욱 ( Dong Kook Kim,Ki Seab Kim,Young Wook Chang ) 한국공업화학회 1996 공업화학 Vol.7 No.5
생분해성 지방족폴리에스터인 poly(butylene succinate) (PBS)의 기계적 물성을 변화시키기 위해서 에스터주쇄 구조에 카보네이트 구조를 도입시킨 에스터-카보네이트 공중합체를 합성하였다. 이 공중합체는 일단계에서 succinicacid와 1,4-butanediol(BD)을 에스터 반응시켜 oligo(butylone succinate)를 합성하였고, 이 단계에서 oligohexamethylenecarbonate diol(OHMCG)을 첨가하고 고진공하에서 축중합하여 제조하였다. 촉매로는 titanium(IV) isopropoxide (TIP)를 사용하였다. 합성된 공중합체의 구조는 FT-IR과 ¹H-NMR을 이용하여 확인하였으며, 열적, 기계적 물성은 각각 DSC와 UTM을 이용하여 조사하였다. 고분자량의 공중합체를 얻을 수 있는 적정 촉매양은 succinic acid에 대해서 lwt% 였다. Diol 혼합물의 몰비(BD:OHMCG)가 249:1∼149:1일 때, 높은 점도를 갖는 공중합체를 얻을 수 있었다. 또한, OHMCG의 함량이 증가함에 따라 공중합체의 용융점은 계속 감소하였다. 혼합물의 몰비가 149:1인 경우의 공중합체의 물성은 PBS에 비해 초기탄성율은 약간 감소하였지만 파단신장율은 2배 정도 증가되었다. An ester-carbonate copolymer was synthesized, in which carbonate was inserted into a biodegradable aliphatic polyester, poly(butylene succinate) (PBS), to modify its mechanical properties. The synthesis was carried out by condensation reactions in two steps. In the first step, oligo(butylene succinate) was prepared by the reaction of succinic acid with 1,4-butanediol (BD). In the second step, it was reacted with oligohexamethylenecarbonate diol (OHMCG) to prepare the ester-carbonate copolymer. Titanium(IV) isopropoxide (TIP) was used as a catalyst for the reaction. The structure of the copolymer was confirmed by FT-IR and ¹H-NMR and the thermal behavior and mechanical properties were investigated by differential scanning calorimetry (DSC) and universal testing machine (UTM), respectively. It was found that optimum amount of the catalyst for the formation of high molecular weight copolymer was lwt% for succinic acid. When the BD:OHMCG is in the range 149:1-249:1, the copolymer with high viscosity was obtained. As the OHMCG content was increased, melting temperature (T_m) of the copolymer was decreased. When BD:OHMCG is 149:1, the copolymer showed a increase in ultimate strain by two times and the slight decrease in modulus compared to those of PBS.
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