김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

시간 경과에 따른 부가중합형 실리콘 인상재의 수소 발생

이근우,김경남,고영무 大韓齒科器材學會 1995 대한치과재료학회지 Vol.22 No.1

Clinical reports have indicated that there occurs many bubbles on the surface of the gypsum model because of the hydrogen release from the addition silicone impression materials. Recently the products containing hydrogen absorber have been being developed but not perfect yet. In this study, 4 type Ⅲ dental stones(New fujirock ; G.C., New Plastone ; G.C., MG dental stone ; Maruishi, Suprastone ; Kerr) was poured into the impression of detail-reproducing test block taken using addition-type silicones (Correct VPS ; J/P, Exafine ; G.C., Express ; 3M, Extrude ; Kerr, Provil ; Bayer, Reprosil ; Dentsply) with the intervals of 0, 15, 30, 45, 60, 90 and 120 minutes. Gypsum model was removed from the impressions and the number of bubbles on the surface of casts was counted after 30 min. Ordinal numbers were given to the ranges of the number of bubbles like this, Score 0 ; 0, 1 ; 1∼10, 2 ; 11∼30, 3 ; 31∼60, 4 ; 61∼100, 5 ; 101∼200, 6 ; 201∼400, 7 ; 401 or more. There was no bubble in Correct VPS impression poured immediately in MG dental stone and Extrude impression poured immediately in New Fujirock, New Plastone, and MG dental stone. Significant bubbles were appeared in Provil until 15 minutes(Score 1∼3), Reprosil until 30 minutes(Score 1∼2), and Express until 45 minutes(Score 1∼2) in all gypsum models(p<0.05). Express impression poured in Suprastone(Score 2) and New Plastone(Score 4) had bubbles until 120 minutes(p<0.05). On the basis of this study it can be concluded that not only hydrogen release but also compatibility with gypsum should be considered when using addition silicones.

소각장별 비산재 중의 중금속 용출 특성 및 존재형태

이우근,김준수,김진범 江原大學校 附設 環境硏究所 1998 環境硏究 Vol.15 No.-

본 연구는 도시폐기물 소각시설에서 발생되는 비산재 중에 함유된 중금속으 존재형태 및 용출 툭성을 알아보기 위해 수행되었다. 용출 실험은 KSLT, TCLP, MALT법을 이용하였고 중금속의 존재형태별 분포에는 다음과 같이 5가지 형태로 구분하였다.; exchangeable, carbonate, reducible, organic, residual fraction.용출 심험 결과에 의하면 pH 4로 일정하게 유지한MALT법에 의한 중금속 용출량이 가장 높 This study was carried out to estimate the fractional composition and leaching properties of heavy metals in fly ash generated from three municipal solid waste incinerators(MSWI). Three leaching tests are used in this work. These are Korea Standard Leaching Test(KSLT)), Toxicity Characteristic Leaching Procedure (TCLP), Maximum Availability Leaching Test(MALT) method. The fractional composition of heavy metals is classified into five fractions; exchangeable, carbonate, reducible, organic, residual fraction. According to the leaching tests, it seems that the highest amounts of heavy metals are leached by MALT method in which pH of 4.0 maintains constantly. From the results of the fractional composition of heavy metals, above 45% of Cd is leached by the change of pH or/and concentrations at I and J incinerators. And 91% of Cd is extracted under the reducible environment at B incinerator. But Cu may be leached under the strong acidic environment. The amount of Cu at B incinerator is less leached than that of I and J incinerators due to high residual fraction. About 35% of Ni in fly ashes at three incinerators exists in reducible fraction. It is probably leached under the reducible environment. Pb may be leached under the weak acidic environment at I and J incinerators, but in case of S incinerator leached under the strong acidic environment.

논에 있어서 포장정보 공간변이의 공간통계학적 해석

이충근,손연규,성제훈,정인규,김상철,박우풍,박원규 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.2

논에 있어서 정밀농업을 위한 기초자료수집과 분석을 하기 위해서 토양의 특성, 포장면 고저차, 생육상태 그리고 수량에 관한 포장정보를 조사한 결과 아래와 같은 결과를 얻었다. 가. 기본통계값을 살펴보면 유효태 규산의 경우는 한 포장내 14배 이상의 변이차이를 보였으며, 생육정보 중 분얼수는 3배, 수량은 4배 이상의 차이를 보였다. 나. 포장정보의 변이계수를 살펴보면 5.45∼51.3%의 공간변이를 보였다. 토양특성 중에 pH를 제외한 나머지가 10%이상의 공간변이를 보였다. 포장면 고저차는 51.3%, 생육정보는 7.32∼23.2%, 그리고 수량에 있어서도 변이계수가 22.5%를 보였다. 다. 포장정보를 공간통계학을 이용해 해석한 결과, 포장정보의 공간구조 발달여부를 표시하는 Q값이 0.24∼1로서 공간구조가 포장정보에 따라서 발달되어 있었으며, 공간변이의 의존거리를 나타내는 랜지는 8.1∼147.9m를 보였다. 그러나, 실질적인 랜지는 토양특성값이 15∼50m 정도, 생육정보는 15m 전후, 포장면 고저차는 30m 정도, 수량은 8.1m를 보였다. 이것을 기초로 하여 크리깅 방법으로 데이터를 보간하여 지도화 시킨결과, 공간변이를 이해하는데 유용하게 이용될 수 있는 포장정보 지도를 얻을 수 있었다. Soil properties, relief of field surface, growth information, grain yield were investigated in a 1ha paddy field in 2001 to obtain basic field information for precision agriculture. The field information were analyzed to examine their within field variability using descriptive statistical method. Semivariograms and Kriged maps of geostatistical analysis were also adopted to examine their within field spatial dependence. The results obtained were as follows ; 1) Nutrient distribution difference of available SiO_2 was seemed 14 times overs, 3 times overs at tiller number, 4 times overs at grain yield from within a paddy field. 2) Descriptive statistics of field information showed that the coefficient of variation ranged from 5.45∼51.3%. 3) Field information showed a high spatial dependence within a paddy field. The Q values ranged from 0.24∼1, the ranges of spatial dependence were from 8.1∼50m, respectively. 4) Kriged maps enable the visualization and comparison of the spatial variability of field informaton.

Honeycomb 觸媒에 의한 窒素酸化物과 2-CP의 同時除去에 관한 硏究

李宇根 江原大學校 附設 環境硏究所 1993 環境硏究 Vol.10 No.-

도시폐기물 소각로에서 배출되는 질소산화물과 Dioxin類를 동시에 제거하기 위해서 honeycomb型 觸媒를 이용하여 반응온도 200-400℃의 범위에서 질소산화물과 Dioxin類의 前?物質인 2-CP의 同時除去 실험을 수행하였다. 실험결과에 따르면 space velocity 6000 hr^(-1)과 NH₃/NO_(x)比 0.5에서 90%이상의 NO_(x)제거율이 얻어졌으며 space velocity 1200 hr^(-1)에 2-CP가 거의 완전히 분해됨을 알 수 있었다. 동시제거 실험에서는 공존물질에 의한 제거율의 저하는 없었고 2-CP의 경우 단독 제거보다 동시제거 실험에서의 제거율이 더 높음을 알 수 있었다. The removal of NO_(x) and 2-CP was investigated over the temperature range of 200-400℃ by using a honeycomb catalyst, for the purpose of finding the condition to decompose simultaneously NO_(x) and dioxin. The removal efficiency of NO_(x) was 90% at the space velocity of 6,000 hr^(-1) and NH₃/NO_(x) ratio of 0.5. And the removal efficiency of 2-DP was 99% at the space velocity of 1200 hr^(-1). There was no effect in the removal efficiency of NO_(x) and 2-CP by co-existing materials(NO_(x), NH₃, 2-CP etc.) at the simultaneous decomposition. The removal efficiency of 2-CP in the simultaneous decomposition was higher than in the only decomposition of 2-CP.

Ehrlich 복수암 세포의 지질대사에 관한 연구

이희성,하창우,이근배 중앙대학교 의과대학 의과학연구소 1977 中央醫大誌 Vol.2 No.1

We have studied on lipids of Ehrlich ascites tumor cells (EATC). Lipids were extracted by Kennedy and Kanfer(1964) method and lipoproteins were separated by paper electrophoresis. For the identification and assay of lipid extracts, thin-layer chromatography was employed. The incorporation of ^32P-orthophosphate into phospholipids of EATC was also observed. The results obtained were summarized as follows; 1. The total lipid content of EATC was 47.15 ㎎ per g wet weight; of this 32.2㎎ per g was neutral lipid and 14.95 ㎎ per g was phospholipid. 2. The amounts of neutral lipid fractions of EATC, i.e., free cholesterol, esterified cholesterol, mono-, di-, triglyceride and free fatty acid were 2.06㎎, 1.75㎎, 4.71㎎, 7.15㎎, 10.74㎎ and 2.21㎎, respectively. The amount of triglyceride of EATC was similar to those of normal mouse liver. 3. Phospholipid composition of EATC was phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and unidentified phospholipid, and contents of the individual phospholipids showed that the over-all composition were very similar to normal mouse liver. 4. Lipoprotein fractions of EATC were chylomicron, β-lipoprotein, α-lipoprotein, and prealbumin. Chylomicron fraction contained a larger amount of lipid and α-lipoprotein fraction contained a smaller amount of lipid. 5. The incorporation of ^32P into phospholipids reached a maximum 5 minutes after incubation.

소각잔사 중의 클로로벤젠과 클로로페놀의 함량 및 이성체 분포특성

이우근,심영숙,김진범 江原大學校 附設 環境硏究所 1998 環境硏究 Vol.15 No.-

Chlorobenzens(CBs) and chlorophenols(CPs) in fly asha discharged from several municipal soild waste incinerators (MSWI) were investigated in terms of total content and isomer distribution. The content of CBs and CPs was highest in the fly ash from S incinerator, which were 1040.7ngCB/g-fly ash and 1156.4ngCP/g-fly ash. According to the experimental results, while all samples contained a large amount of the higher chlorinated CBs the amount of lower chlorinated CPs varied with season It was found that the isomer distribution of CBs and CPs was almost constant regardless of season. The quantities of CBs and CPs depended closely on the chemical composition of MSW, while the isomer distribution of CBs and CPs was contant throughout all the year round.

인체 Calbindin-D9k 항체 제작과 종특이적 활성

이근우, 홍의주, 이근식, Vu-Hoang Dang, 정의배 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.2

본문참고

부산광역시의 대기질 변화경향 및 특성에 관한 연구

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