Polyethyleneglycol을 이용한 Immunoglobulin 정량법

房極勝,崔震浹,金正基 東亞大學校 大學院 1984 大學院論文集 Vol.8 No.2

A single and rapid method for quantative determination of immunoglobulin in serum is described. The method uses polyethyleneglycol which causes precipitation of immunoglobulin and investigation into electrophorectic pattern of serum by polyethyleneglycol and correlation between total protein concentration and immunoglobulin concentration have been performed. The polyethyleneglycol precipitation test described provides a rapid, accurate and precise way to measure immunoglobulin.

組大豆 Lecithin의 飼料化에 관한 硏究

金榮吉,房極勝,金大鎭 東亞大學校 大學院 1978 大學院論文集 Vol.2 No.-

The Research was conducted to study the feeding value of crude lecithin produced as by product of soybean oil manufacturing. The results obtined were as follows. 1. The chemical composition, mineral composition vitamin contents, gross energy and metabolizable energy values of crude lecithin were determined. 2. No significant difference was observed for the body weight gain of chicks between control and lecithin groups of 3%, 6%,9% and 12% addition. 3. Significant difference(p<0.05) was observed for feed intake between lecithin 12% and lecithin 6% and 9% group. However, No significant difference was found among control, lecithin 3%.6% and 9% groups. 4. No difference was found for feed requirement between treatments. However, the lowest figure was 2.32 for lecithin 9% treatment, followed by 2.40 of lecithin 6% and 2.41 of lecithin 3%, orderly. 5. No difference was found between treatments for RBC, WBC, hemoglobin and hematocrit value. 6. The composition of moisture, crude protein and ether extract of chicks ranged from 71.2 to 72.15%, from 15.93 to 16.45%, and from 6.19 to 7.05%, respectively. 7. The weight of abdominal fat and liver fat of chicks ranged from 42.4 to 63.7gm. and from 2.2 to 2.4gm, respectively, showing insignificant difference. 8. Total of 9 feed manufacturers used lecithin as energy source for poultry ration and each manufacturer employed somewhat different mixing method. 9. The patented bleaching reagents of crude lecithin were hydrogen peroxide and dibenzoyl peroxide. The bleaching facilities of crude lecithin with the capacity of 2M/T per day was calculated to amount to 3,650,000won.

在來山羊의 飼育能力 向上에 관한 硏究

崔暻文,金榮吉,房極勝 東亞大學校 大學院 1977 大學院論文集 Vol.1 No.-

Experiment were conducted on Korean Native Goat to ascertain the effect of ovine coccidiosis on feedlot Kids. The results obtained were summarized as follows: 1. Oocyst production in each groups of kids reached level of 3 and 4 million oocyst per gram feces 18 to 22 days after kids entered in feedlot. 2. The parasitic development of coccidia was suppressed by SDM significantly (P 0.01) within 1 weeks after drug administration. 3. Hemoglobin and hematocrit value were altered in affected kids but changes in group average were not statistically significant. Total serum protein concentraion were decreased steadly throughout experiment period though SDM suppressed oocyst development within 1 week. 4. Adequate immunity did not develop in any of kids under 6 months old when challange inoculated with 500 thousand oocyst.

Influence of Transferred Anti-Nematospiroides dubius Immune Serum and IgG on Immune Reaction in Recipient Mice

Keuk Seung Bang(방극승) 대한기생충학열대의학회 1982 The Korean Journal of Parasitology Vol.20 No.2

[P10-238] Antioxidant Activities Ascorbic Acid and Total Phenolic Content of Korean Honey

Keuk Seung Bang,Yong Ho Cha,Young gil Kim,Dae jin Kim,Kyung Jin Lee 한국식품영양과학회 2006 한국식품영양과학회 학술대회발표집 Vol.2006 No.10

신단백질(新蛋白質) 사료(飼料)의 개발(開發) 및 이용효율(利用效率)에 관(關)한 연구(硏究) -1. 재래산양(在來山羊)에 유용(有用)한 비단백태실소(非蛋白態室素)의 개발(開發)에 관(關)한 연구(硏究)-

방극승 ( Keuk Seung Bang ),김영길 ( Young Kil Kim ) 東亞大學校附設 石堂傳統文化硏究院 1978 石堂論叢 Vol.2 No.-

우수한 비단백대 질소를 개발 在來山羊에 有用하게 이용하고자 뇨소 피복뇨소 및 DUIB의 안전성과 적응성을 검토한 바 다음과 같은 結論을 얻었다. 1. 胃液의 pH는 NPN투여 1시간 후 최대치를 보였으며 뇨소 피복뇨소는 Alkali化하는 경향이었으나 DUIB는 별 영향이 없었다. 2. 胃液의 암모니아 농도는 體重 kg當 피복뇨소 0.77gm DUIB 1.28gm 까지 안전하였으며 뇨소 0.3gm도 약간의 위험성이 있었다. 3. 血液의 암모니아 농도는 體重 kg當 뇨소 .03gm 피복뇨소 0.77gm DUIB 1.28gm까지 안전하였다. 4. 在來山羊은 體重 kg當 뇨소 0.9gm 피복뇨소 2.33gm을 투여시 40분부터 80分 사이에 심한 중독증상을 나타냈다. 5. 뇨소 0.3gm(체중 kg當) 피복뇨소 0.77gm을 4주간 급여 한 바 胃液의 pH와 혈액의 암모니아 농도는 감소하였다. (P<0.05) 6. 以上의 結果로 볼 때 DUIB는 가장 우수한 非蛋白態 室素로 開發되어야 할 것이다. This experiment was conducted in effort to determine safety and proper amount of Urea, Coating urea and Diuredo-isobuane (DUIB) in Korean Native Goats. The results obtained were as follows: 1. Ruminal pH values were considerably higher (7.0-8.0) on urea and coating urea treated goats. But constant ruminal pH values were recorded on DUIB treated goats. 2. Ruminal ammonium nitrogen concentration were below toxic levels when treated with coating urea (0.77 gm/kg B. W.) and DUIB (1.28 gm/kg B. V.). Although urea (0.3 gm/kg B. W.) were slightly above toxic levels. 3. Blood ammonium nitrogen concentration were below toxic levels when treated with urea (0.3 gm/kg B. W.) coating urea (0.77 gm/kg B. W.)and DUIB (1.28 gm/B. W.) 4. Korean Native Goats used were intoxicated by 0.9 gm urea per kg B. W. and 2.33 gm coating urea per kg B. W. between 40 minutes and 80 minutes after administration. 5. Initial rise in ruminal pH and blood ammonium nitrogen concentration was a tendency for a decline after 4 weeks feeding urea (0.3 gm/kg B. W.) and coating urea (0.77 gm/kg B. W.) (p<0.05). The present data reveal that DUIB is most efficient NPN resource in korean Native Goats.

新蛋白質 飼料의 開發 및 利用效率에 關한 硏究

房極勝,金榮吉 東亞大學校 石堂學術硏究奬勵會 1977 石堂論叢 Vol.2 No.-

This experiment was conducted in effort to determine safety and proper amount of Urea, Coating urea and Diuredo-isobuane (DUIB) in Korean Native Goats. The results obtained were as follows: 1. Ruminal pH values were considerably higher (7.0-8.0) on area and coating area treated goats. But constant ruminal pH values were recorded on DUIB treated goats. 2. Ruminal ammonium nitrogen concentration were below toxic levels when treated with coating urea (0.77 gm/kg B.W.) and DUIB (1.28 gm/kg B.W.). Although urea (0.3 gm/kg B.W.) were slightly above toxic levels. 3. Blood ammonium nitrogen concentration were below toxic levels when treated with urea (0.3 gm/kg B.W.) coating urea (0.77 gm/kg B.W.) and DUIB (1.28 gm/kg B.W.) 4. Korean Native Goats used were intoxicated by 0.9 gm urea per kg B.W. and 2.33 gm coating urea per kg B.W. between 40 minutes and 80 minutes after administration. 5. Initial rise in ruminal pH and blood ammonium nitrogen concentration was a tendency for a decline after 4 weeks feeding urea (0.3 gm/kg B.W.) and coating urea (0.77 gm/kg B.W.) (p<0.05). The present data reveal that DUIB is most efficient NPN resource in Korean Native Goats.

宿主의 性Hormone이 소진드기 Boophilus microplus의 산란에 미치는 영향에 대한 실험적 연구

房極勝 東亞大學校 1975 東亞論叢 Vol.12 No.2

性 Hormone齊를 소진드기 Boophilus microplus에 주사 하여 본 결과 다음과 같다. 1. Estrogen은 産卵前 휴식기간을 연장하고 산란을 억제 하는 效果가 있었다. 2. Progestron은 産卵活動에 뚜렷한 영향을 주지 않았다. 3. Testosterone 은 진드기의 初期 産卵을 촉진하는 效果가 있었다. 4. 性 Hormone이 産卵 지속기간, 産卵한 卵數에는 영향을 주지 않는 것으로 나타났다. This study was conducted to study the effect of host sexual hormone on egg laying of cattle tick. Boophilus microplus. The results were as follows: 1. Female sex hormone, Estradiol, markedly inhibited egg laying and resting period for egg laying. 2. Female sex hormone, Progestrone, showed no significant effect on egg laying activity. 3. Male sex hormone, Testosterone, acclerated egg laying especially in the early stage of egg laying period. 4. Above three sexual hormone showed no significant effect on the number of egg laid and duration of egg laying period.

Glycan structure and serum half-life of recombinant CTLA4Ig, an immunosuppressive agent, expressed in suspension-cultured rice cells with coexpression of human β1,4-galactosyltransferase and human CTLA4Ig

Kang, Seung Hoon,Jung, Hahn Sun,Lee, Song Jae,Park, Cheon Ik,Lim, Sang Min,Park, Heajin,Kim, Byung Sun,Na, Kwang Heum,Han, Gyeong Jin,Bae, Jae Woo,Park, Hyun Joo,Bang, Keuk Chan,Park, Byung Tae,Hwang, Springer-Verlag 2015 GLYCOCONJUGATE JOURNAL Vol.32 No.3

Sourdough 첨가 보리식빵의 물성적 특성

김경자,홍정훈,방극송 한국조리과학회 2000 한국식품조리과학회지 Vol.16 No.4

1. 보리에서 분리한 균주는 Gram 양성의 구균으로 미생물 동정기를 이용하여 동정해 본 결과 Enterococcus mundtii와 89%의 상동성을 보여 이 균주는 Enterococcus속으로 동정되었다. 2. 초기 pH는 모두 5.5였으며 시간이 경과함에 따라 가장 먼저 pH가 떨어지기 시작한 것은 Lactobacillus sanfrancisco이였으며 다음으로 Enterococcus mundtii의 순이였다. pH 4.5까지 떨어지는데 걸리는 시간도 Lactobacillus sanfrancisco이 8시간 10분으로 가장 짧았으며 Enterococcus mundtii는 9시간 30분이였으며 Lactobacillus plantarum은 9시간 50분의 순이었다. 동일한 pH에서 산도가 높았으며 Enterococcus mundtii, Lactobacillus plantarum의 순이었다. 3. 대조군에 비해 균주가 혼합된 식빵 3개 모두 부피가 커졌으며 이중 Lactobacillus sanfrancisco가 가장 부피가 컸으며 대조군에 비해 11%정도 부피가 증가하였다. 부피를 무게로 나눈 용적비도 부피와 같은 경향을 보였다. 식빵의 높이는 Lactobacillus sanfrancisco 와 Enterococcus mundtii 가장 컸고 다음이 Lactobacillus plantarum, 대조군의 순이었다. 4. 96시간 경과 시 대조군에만 곰팡이가 발견되었고 다른 군에서는 144시간 이후에도 곰팡이가 발견되지 않았다. 경도는 시간경과에 따라 지속적으로 증가하였으며 경도가 가장 낮은 Lactobacillus sanfrancisco와 다음으로 낮은 Enterococcus mundtii의 경우 초기에는 경도 변화가 별로 없었다가 48시간 경과 시 경도가 증가하였다. To investigate the effect of sourdough on the quality characteristics of barley bread, sourdough starter with Lactobacillus plantarum, Lactobacillus sanfrancisco and Enterococcus mundtii was used for baking after 48 hours incubation at 37℃. The specific volume of the bread containing sourdough starter was greater than that the control. Retarding of the firmness was observed in the bread with Lactobacillus sanfrancisco compared with the control and other breads. Above results suggest that sourdough starter fermented by Lactobacillus can be used as natural bread improver.