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- 저자 : Keji Jiang (검색결과 4 건)
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Huang, Zhuoshi,Jiang, Keji,Pi, Yan,Hou, Rong,Liao, Zhihua,Cao, Ying,Han, Xu,Wang, Qian,Sun, Xiaofen,Tang, Kexuan Korean Society for Biochemistry and Molecular Biol 2007 Journal of biochemistry and molecular biology Vol.40 No.5
The enzyme squalene synthase (EC 2.5.1.21) catalyzes a reductive dimerization of two farnesyl diphosphate (FPP) molecules into squalene, a key precursor for the sterol and triterpene biosynthesis. A full-length cDNA encoding squalene synthase (designated as TcSqS) was isolated from Taxus cuspidata, a kind of important medicinal plants producing potent anti-cancer drug, taxol. The full-length cDNA of TcSqS was 1765 bp and contained a 1230 bp open reading frame (ORF) encoding a polypeptide of 409 amino acids. Bioinformatic analysis revealed that the deduced TcSqS protein had high similarity with other plant squalene synthases and a predicted crystal structure similar to other class I isoprenoid biosynthetic enzymes. Southern blot analysis revealed that there was one copy of TcSqS gene in the genome of T. cuspidata. Semi-quantitative RT-PCR analysis and northern blotting analysis showed that TcSqS expressed constitutively in all tested tissues, with the highest expression in roots. The promoter region of TcSqS was also isolated by genomic walking and analysis showed that several cis-acting elements were present in the promoter region. The results of treatment experiments by different signaling components including methyl-jasmonate, salicylic acid and gibberellin revealed that the TcSqS expression level of treated cells had a prominent diversity to that of control, which was consistent with the prediction results of TcSqS promoter region in the PlantCARE database.
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Anglu Shen,Zengling Ma,Keji Jiang,Daoji Li 한국해양과학기술원 2016 Ocean science journal Vol.51 No.4
To explore the effects of temperature changes on dinoflagellate bloom succession in the coastal waters of the East China Sea, changes in the growth, photophysiology, and Rubisco gene expression of Prorocentrum donghaiense and Karenia mikimotoi, two harmful algal species, were investigated at different temperatures (16 to 28°C). The maximal specific growth rate and the maximal mRNA expression of Rubisco gene in P. donghaiense and K. mikimotoi occurred at 20 and 24°C, respectively. The photosynthetic activity of P. donghaiense was generally stable, but K. mikimotoi photosynthesis increased when temperatures rose from 16 to 28°C. The effective photochemical efficiency (Fq'/Fm') and the maximal relative electron transfer rate (rETRmax) of K. mikimotoi increased significantly with increasing temperature, and the lowest and highest values occurred at 16 and 28°C, respectively. It seems that P. donghaiense has higher photosynthetic capacity than K. mikimotoi due to its higher Fq'/Fm', rETRmax, and photosynthetic efficiency (α). However, K. mikimotoi has a higher growth rate than P. donghaiense. These results suggest that the photosynthetic activity and genetic responses of dinoflagellates are species-dependent. It is likely that temperature changes affect species composition during blooms, leading to the observed patterns of bloom succession.
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An optimized procedure for detection of genetically modified DNA in refined vegetable oils
Yuzhu Duan,Yan Pi,Changwen Li,Keji Jiang 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.1
In this study, the amplifiable DNA from refinedvegetable oils was isolated by using commercial DNAextraction kits based on the CTAB method in combinationwith nucleic acid enrichment, and then the presence ofgenetically modified (GM) soybean and maize DNA in theoils was traced by PCR. The results showed that theduration and intensity of heating had no significant effecton the DNA stability and concentration in oils for a shortperiod, suggesting that DNA in oils could be stablyreserved for a certain time, thus making it possible to tracedown refined vegetable oils reliably and effectively. Theresults provided a set of primers suitable for systematicGM oil detection. More importantly, this study made animportant contribution to the economical and reliabledetection of GM vegetable oils regarding food authenticityissues.
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Jinsong Liang,Qibao Xie,Wenjie Feng,Bo Li,Tao Peng,Kejie Liu,Mengjin Jiang 한국섬유공학회 2023 Fibers and polymers Vol.24 No.11
The spinnability of semi-dilute poly(p-phenylene-benzimidazole-terephthamide) (PBIA) spinning solution was regulated by a small amount of polyethylene glycol (PEG) to make it suitable for dry-jet wet spinning. The effects of different molecular weights and contents of PEG on the rheological properties and spinnability of PBIA spinning solution were investigated. Results show that the PBIA spinning solution with PEG 50,000 has the highest viscosity and the best spinnability, which can be smoothly spun by dry-jet wet spinning. The dynamic viscoelasticity study shows that the energy storage modulus and loss modulus of the PBIA spinning solution keeps increasing with PEG 50,000 and achieves the maximum value of 0.5% wt. The maximum draw ratio between the first roll and the spinneret (Dm) also reached a maximum value of 3.0 when the addition of PEG 50,000 reached 0.5% wt. Moreover, primary PBIA fibers prepared by dry-jet wet spinning and a higher draw ratio have a smoother surface appearance and better mechanical properties.
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