백서 담관폐쇄에 의해 증식된 세담관의 조직기원에 관한 연구

기근홍,전호종 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.2

Bile ductules are lined by flat or cuboidal epithelium connecting bile canaliculi between hepatocytes in the portal tracts. These bile ductule were proliferated in obstructive disease of hilary tract. But, the histogenetical origin of these hyperplastic bile ductules following biliary obstruction has not been established. Some people assume that they originate from liver cells, others think they are derived from preexisting ducts, and by anothers to be developed from intermediate cell types between biliary epithelium and hepatocytes. An experimentally induced biliary obstruction in the rat by ligation of common bile duct, and bile ductules was assesed by light microscopy, immumohisto- chemistry, and electron microscopy. The results were obtained that a biliary cirrhosis with proliferating ductules developed 21 days after ligation of bile duct and these were identical to preexisting bile ductules in ultrastructural observation. And also, numerous mitosis of bile ductules were observed, It was concluded that hyperplastic biliary ductules were derived from biliary epithelial cells in preexisting bile ductules vy cell division.

장기에 따른 편평상피암의 Cytokeratin 발현에 관한 연구

기근홍,김창원,김용임 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.1

Keratin is a major class of epithelial intermediate filaments and comprises a group of at least 20 different multigene derived proteins. It is expressed in different epithelia with specific combinations. The expression of cytokeratins (CK) 1.5/6, 7, 8, 10, 13, 17, 18, 19, and 20 in 35 cases of squamous cell carcinomas from uterine cervix (5 cases), lung (5 cases), esophagus (5 cases), skin (5 cases), oral cavity (5 cases), and metastatic Iymph nodes (2 cases of each organs) were examined. Some cases were examined by electron microscopy. The results obtained is as follows. 1. The CK 1 positive tumors is from keratinizing skin tumor and is show weak positivity. The CK 5/6 positlve tumor is from uterine cervix, lung and oral cavity. The CK 8 positive tumor is from uterine cervix, lung, esophagus, skin and oral cavity. The CK 13 positive tumors is from uterine cervix and lung. The CK 17 show positive staining in uterine cervix and lung. The CK 18 positive tumors is from uterine cervix, lung, esophagus, skin, and oral cavity. The CK 19 positive tumors is from uterine cervix, lung, esophagus, skin, and oral cavity. The CK 20 shows no reactivity in all cases. 2. Ultrastructurally, the tumor cells showed bundles of tonofibriIs, electron-dense tonofilaments, and desmosomes. In conclusion, CK 1,8 18 and 19 did not show orgn specificity. So it used simple epithelial markers in the majority of squamous cell carcinomas. The absence of CK 20 immunoreactivity may helpful in differential diagnosis of squamous cell tumors.

갑상선 질환에서의 부여포세포 표현 양상

기근홍,이학봉,신은택 조선대학교 부설 의학연구소 1998 The Medical Journal of Chosun University Vol.23 No.2

The parafollicular C cell is a neuroendocrine cells in the thyroid gland. It secrete the calcitonin and regulate the serum level of calcium. The materials for this study consisted of 20 cases of adenomatous goiter 10 chronic lymphocytic thyroiditis, 5 Hashimoto's thyroiditis, 10 follicular adenoma, 20 papillary carcinoma, and 5 follicular carcinoma that resected for definite treatment of thyroid disease at Chosun University Hospital from January, 1995 to December, 1998. All cases were performed of immunohistochemical stain for antimouse monoclonal calcitonin, chromogranin A and neuron-specific enolase (NSE) antibodies. The result obtained as follows. Immunohistochemically, the positive cells for calcitonin, chromogranin A and calcitonin were increased and intensely stained in the chronic lymphocytic thyroiditis and Hashimoto's thyroiditis. In the follicular adenoma, papillary carcinoma and follicular carcinoma, the C cells were increased around the tumor cell nests. But, the tumor cells showed negative staining. In conclusion, the calcitonin-producing neuroendocrine cells were showed in most thyroid diseases and were increased in inflammatory diseases. This finding suggest that the neuroendocrine cells act as protection to injurious agents or neoplastic transformation.

소장에서 발생한 원발성 편평세포암종 1예 : Report of a case and review of the literature

기근홍,이동명 조선대학교 부설 의학연구소 1999 The Medical Journal of Chosun University Vol.24 No.2

Small bowel cancer represents 1 to 2 percent of all gastrointestinal malignancies. By far, most small bowel epithelial tumors are metastatic. We report an unusual case of primary squamous cell carcinoma of jejunum. The patient was a 69-year-old man who suffered from whole abdominal pain and transferred to our hospital for operation. There was a perforation at the jejunum which was 100cm apart from the ileocecal valve. There was a 5×4m sized ulceroinfiltrative tumor with a perforation. Histologically, it was revealed that the tumor had nests of keratinizing squamous cell carcinoma involving mainly the submucosa and the muscular layer. The overlying mucosa was eroded. The patient had no underlying duplication or inflammatory disorder. A colon study, gastrofibroscopy, computed tomogram of the chest and abdomen, and laryngoscopic exam were performed and revealed no evidence of malignancy. Also there was no evidence of metastatic lesions anywhere.

HPV에 감염된 자궁경부 상피세포의 Keratin 아형 변화에 관한 연구

기근홍,이영미,장원재,임용,임성철,김창원,전호종 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.2

The human papillomavirus (HPV) are wide spread in the world, causing proliferation of epidermal and mucosal surface. Certain papillomaviral types are oncogenic in vivo and in vitro. HPV DNA has been detected in most of cervical lesions such as condyloma acuminata, dysplasias, and invasive carcinomas. The materials for this study consisted of 5 dysplasias. 5 squamous cell carcinomas, and 5 exocervix for control, that resected for definite treatment of uterine disease at Chosen University Hospital from January, 1991 to December, 1993. All cases were performed of in situ hybridization for HPV DNA type 6/11, 16, and 18. And also immununohistochemical stain for panel of monoclonal cytokeratin (CK) antibodies were done. The result obtained is as fallows : 1. HPV DNA type 6/11 are detected in all dysplasias. HPV DNA type 16 and 18 are detected in squamous cell carcinomas. 2. Stratifed squamous epithelium of exocervix are positive staining for CK 1, CK 5/6, CK 8, CK 13, CK 14, and CK 19. HPV DNA type 6/11 infected cells are positive for CK 1, CK 13, CK 14, CK 17, CK 18, and CK 19. HPV DNA type 16/18 infected cells are positive for CK 1, CK 5/6, CK 10, CK 13, CK 14, CK 17, CK 18, and CK 19. In conclusion, HPV infection of uterine cervix is associated with change of normal cytokeratin expression pattern. Dysplastic cells are changed of CK 5/6, CK 8, CK 17 and CK18. Squamous cell carcinoma cells are changed of CK 8, CK 17, and CK 18.

무정자증 환자에 있어서 고환내 Leydig 세포의 변화에 관한 초미세형태학적 연구

기근홍,문현준,최봉남 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

The Leydig cells are identified in a peritubular boundary area of the testicular interstitium. These cells are differentiated in situ from myoid cells within the boundary layer and invaded into the interstitial tissue at puberty. The materials for study consisits of twenty cases of azospermia with cryptorchidism confirmed by sperm analysis at Chosun University Hospital. All cases were performed by hematoxylineosin stain and PAS stain for detection of Leydig cells, and ultrastructural abservation for change of cytoplasmic organelles in azospermic patients. The results obtained are follows. The leydig cells in the interstitial tissue of azospermic testis forming large clusters and showing hypertrophic change. Within the cytoplasm, numerous dilated endoplasmic reticulum, disrrupted mitochondria and osmiophilic round bodies are seen. A few myoid cells within the boundary layer are hypertrophied and show an intermediate pattern between myoid cells and Leydig cells. There are unusual cells, either single or clusters, within the thickened boundary layer. They can be identified as Leydig cells similar to the ordinary Leydig cells in the interstitial tissue. This findings indicated that ordinary function of Leydig cells are intact, but it is thought that the decreased function may be due to damage of the testis in cryptorchidism.

末梢神經腫瘍에서 GFAP의 免疫組織化學的 表現에 關한 硏究

기근홍,조국형,범진선 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.2

Glial fibrillary acidic protein (GFAP) is one of the intermediate filament proteins, It is avaUable for identification of tumors glial cell origin in central nervous system. The specialized cells of the peripheral nervous system could express GFAP. The materials for our study consisted of 25 neurofibroma and 15 neurilemmoma, that resected for diagnosis or definite treatment at Chosun University Hospital from January, 1989 to December, 1991. All cases were performed of immunohistochemical stain for GFAP, vimentin and S-100 protein by ABC method. Immunoreactivity was evaluated and compared with the reactivity of GFAP, vimentin and S-100 protein. All cases of neurofibroma and neurilemmoma was positively stained in the neoplastic cells to the vimentin and S-100 protein. GFAP were positively stained in 10 of 25 neurofibroma (40%) and 11 of 15 neurilemmoma (73%). In the neurilemmoma, the positive cells were found primarily in cellular area, rarely in verocay bodies. Dermal neurofibromas were negative for GFAP, except of deeply located plexiform neurofibromas. Positive tumor cells were usually bipolar and spindle-shaped. The GFAP positive tumor cells were appeared around in hyalinized vessels in many cases.

자궁경부 상피내병변에서 HPV와 p53 단백의 변화에 대한 면역조직화학적 소견

기근홍,황철기,신향미 조선대학교 부설 의학연구소 2000 The Medical Journal of Chosun University Vol.25 No.1

Background and Objectives : p53 is a tumor suppressor gene. Loss of function of the p53 tumor suppressor gene implicated in a wide variety of human tumors. Many mechanisms are involved in p53 dysfunction. One of the mechanisms is binding of oncogenic virus such as human papillomavirus (HPV). HPV infection are strongly linked to the development of cervical neoplasia including cervical intraepithelial neoplasia (CIN) of uterine cervix The purpose of this study is to investigate the relationship between aberrant p53 expression and presence of HPV DNA in CIN of the uterine cervix. Materials and Methods : In the present study, the author analyzed 35 cases of paraffin-embedded CIN, including 10 cases of grade 1, 10 cases of grade Ⅱ, and 15 cases of grade Ⅲ CIN by the immunohistochemistry and in situ hybridization. Results : Overall positive rate of HPV DNA type 16 and type 18 was 74.3% (26 cases) and 65,7% (23 cases), respectively. Nuclear accumulation of p53 was found in 22 cases (62.9%) of all CIN. In negative cases of HPV DNA type 16, the p53 was positive in 18 cases and negative in 8 cases. In positive cases of HPV DNA type 18, the p53 was positive in 16 cases and negative in 7 cases. In negative cases of HPV DNA type 16, the pS5 was positive in 6 cases and negative in 6cases. Conclusion : This results suggest that HPV infection may contribute to the DNA damage associated with the accumulation of aberrant p53 protein, and then closely relate to the progression of cervical neoplasms. But, alteration of p53 Protein levels and presence of HPY DNA was not an exclusive markers of cervical tumorigenesis.

자궁의 악성 혼합성 뮬러 종양 : 1예 보고 report of a case

기근홍,노광을,이민전 조선대학교 1993 The Medical Journal of Chosun University Vol.18 No.2

Malignant mixed mullerian tumor is rare tumor of the uterus. This tumor derived from mullerian mesoderm, but their histogenesis is still controversial. We report a case of homologous type of malignant mixed mullerian tumor of the uterus in 64 year-old female. The tumor composed of adencocarcinoma and leiomyosarcoma. After radical hysterectomy, this tumor is metastasize to lung 5 months later.

성상세포종에서 등급에 따른 p53 단백 발현의 차이

기근홍,양정원,이승학,이민오,성정희 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1

Background and Objectives: Mutation or inactivation of the p53 is important mechanism in a wide variety of human tumors. The purpose of this study is to investigate the relationship between expression amount of p53 protein and the grade of astrocytic gliomas. Materials and Methods: This study analyzes 40 paraffin-embedded astrocytic glioma, including 10 cases of low grade, 20 cases of high grade, and 10 cases of maligant glioma (glioblastoma multiforme) were used to analyze the status of abnormal accumulation of wild type of p53 protein. The evaluation of the immunostaining result was based on the percentage of positive neoplastic cells. Results: Average positive cell rate of p53 protein were 4.2%, 35.4% and 16.2% in low grade astrocytoma, high grade astrocytoma and glioblastoma multiforme, respectively. Conclusion: p53 gene mutations were correlated with histologic grade of glial tumors. High grade and glioblastoma multiforme showed higher positive cell rate than low grade astrocytoma. These results suggest that the high positive rates of p53 protein is related to the poor prognosis.