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Eco RI Restriction Fragment Length Polymorphism of L - myc gene has arisen by a single base change
Na, Doe Sun,Susumu Nishimura,Kazuko Kawashima 한국유전학회 1988 Genes & Genomics Vol.10 No.4
Human genomic DNA shows an EcoRI restriction fragment length polymorphism (RFLP) of L-myc. defined by 10 and 6 kilobase EcoRI fragments. Recently, it has been found that the RFLP of L-myc gene was closely correlated with the progression of human lung cancer to the metastasis. To investigate the differences of the genetic information between two ECoRI fragments, we have isolated clones of these genes from a genomic library. The genomic library was prepared using the DNA isolated from the tumor tissue of a lung cancer patient. The 10Kb and 6Kb L-myc genes were mapped using several restriction enzymes. Comparison of the restriction map of 10Kb L-myc gene to that of 6Kb gene showed that the 6Kb gene may have arisen by the mutation of the 10Kb gene leading to the creation of a EcoRI site. Nucleotide sequence analysis of both genes in this region revealed that a single base change from C to A in 10Kb gene resulted in a creation of a ECoRI site.
Identification of Trichophyton tonsurans by Random Ampli6ed Polymorphic DNA
Kim, Jeong Aee,Gusmao, Norma Buarque de,Okada, Kaoru,Takaki, Galba Maria de Campos,Fukushima, Kazutaka,Nishimura, Kazuko,Miyaji, Makoto 대한피부과학회 1999 Annals of Dermatology Vol.11 No.3
Background : T. tonsurans is an anthropophilic dermatophyte mostly causing tinea capitis and tinea corporis. In East Asian countries, it has rarely been isolated until now. However, it is necessary for researchers in Asian countries to be more accustomed to T. tonsurans than before because of frequent international sports exchanges nowadays. Objectives : This study was performed to identify T. tonsurans by random amplified polymorphic DNA (RAPD) analysis. Methods : Fifteen strains which were tentatively identified as T. tonsurans in Brazil were identified again by several conventional mycological tests and RAPD analysis. Results : Among 15 Brazilian strains, 3 were identified as T. tonsurans, 8 T. mentagrophytes, 3 T. rubrum and 1 T. raubitschekii by conventional mycological tests. This result was examined again by RAPD analysis. Conclusion : RAPD analysis is considered a rapid and reliable method for identification of T. tonsurans if the procedure is carefully standardized with adequate primers.