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        Expression of antimicrobial peptide snakin‑1 confers efective protection in rice against sheath blight pathogen, Rhizoctonia solani

        Kaushik Das,Karabi Datta,Sailendra Nath Sarkar,Swapan Kumar Datta 한국식물생명공학회 2021 Plant biotechnology reports Vol.15 No.1

        Rice sheath blight disease caused by the necrotrophic fungus Rhizoctonia solani, is an economically detrimental fungal disease which signifcantly afects rice productivity worldwide. Potato snakin-1, a cysteine-rich basic antimicrobial peptide (AMP), is a member of the novel Snakin AMP family. In order to assess the ability of the snakin-1 peptide in protecting rice against the sheath blight disease, we have developed transgenic rice constitutively expressing the snakin-1 peptide. The antimicrobial activity of snakin-1 was evaluated against the sheath blight pathogen Rhizoctonia solani both in vitro and in planta. Crude protein from transgenic rice leaves showed in vitro antifungal activity against Rhizoctonia solani. Moreover, in planta bioassay results also confrmed the same, wherein snakin-1 expressing rice plants showed signifcantly enhanced protection against the sheath blight disease. This report demonstrates how a member of the Snakin family of antimicrobial peptides has been successfully used to generate sheath blight resistance in rice, without compromising on its agronomic characteristics and at no phenotypic cost.

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        Exclusive T2 MRI contrast enhancement by mesoporous carbon framework encapsulated manganese oxide nanoparticles

        Kashmiri Deka,Anupam Guleria,Dinesh Kumar,Jayeeta Biswas,Saurabh Lodha,Som Datta Kaushik,Suman Dasgupta,PRITAM DEB 한국물리학회 2020 Current Applied Physics Vol.20 No.1

        Single mode (either T1 or T2) contrast agents employed during magnetic resonance imaging owe their advantage over their dual counterparts to the fact that they do not involve any quenching caused by interference between the two modes. The chemistry involving oxides of manganese is highly significant due to their applicability as MRI contrast agents. Manganese oxides are usually known to display a dominant T1 relaxation enhancement. But, in this work, an engineered structure of manganese oxide (Mn2O3) nanoparticles encapsulated within mesoporous carbon frameworks was developed which exhibited dominant T2 contrast enhancement, through regulation of contact between the magnetic ion and water. Microstructural characterization revealed that the mesoporous carbon frameworks were spherical in shape and the nanoparticles within them had an average size of 40–50 nm. Relaxivity measurement, MRI experiments and cell viability assay convincingly established the system as a new class of biocompatible T2 based magnetic resonance imaging agent.

      • KCI등재

        Climate variability, livelihoods and social inequities: The vulnerability of migrant workers in Indian cities

        Sunil D Santha,Surinder Jaswal,Devisha Sasidevan,Ajmal Khan,Kaushik Datta,Annu Kuruvilla 한국외국어대학교 국제지역연구센터 2016 International Area Studies Review Vol.19 No.1

        This paper examines the vulnerability context of migrant workers in the informal sector in three Indian cities (Kochi, Surat and Mumbai), specifically in terms of how their livelihoods interface with climate variability, related hazard events and social inequities. It examines the progression of population vulnerability from a political economy perspective. The underlying assumption is that migrant workers’ vulnerability to climate variability in cities is closely embedded within the wider political economy of their day-to-day livelihood struggles. A mixed methodology design was adopted to carry out the study. Data from 50 migrants in each of the three cities were collected using a semi-structured interview schedule. The research demonstrates that urban vulnerability is a condition that shapes and reshapes itself continuously and fiercely, accompanied by the processes and structures of unplanned rapid urbanisation, environmental change and social exclusion. A significant feature of migrant workers’ vulnerability is its multi-dimensionality. This paper suggests that urban planners involved in environmental governance and risk reduction have to critically reflect on certain terminologies and practices in the field of climate change, while addressing these multi-dimensionalities.

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