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장인권,Ayyaru Gopalakannan,Kannan Suriakala,김종식,김봉래,조영록,Xian-Hong Meng,서형철 한국수산과학회 2008 Fisheries and Aquatic Sciences Vol.11 No.4
Viral diseases are major emerging problems of shrimp that have affected the production, and even complete losses for shrimp farms. In this study, we developed a sensitive TaqMan real-time PCR method to quantify white spot syndrome virus (WSSV) and hepatopancreatic parvovirus (HPV) in the shrimp and pond water in which fleshy shrimp, Fenneropenaeus chinensis, and Pacific white shrimp, Litopenaeus vannamei, are reared. WSSV and HPV in pond seawaters ranged from 1.65×103 to 2.43×109 and from 0 to 4.43×105 copies/L of seawater, respectively. Of 20 ponds analyzed, all pond water and shrimp were positive for WSSV. L. vannamei showed higher susceptibility to WSSV than F. chinensis. HPV was detected only in the pond water for F. chinensis. In shrimp tissue, however, HPV was found in both species, with 23-times higher infection rate in F. chinensis than L. vannamei. The total bacterial counts in the pond water ranged from 2.23×103 to 1.98×105 CFU/mL. The variations in total bacterial count for each pond appeared to correlate to the variations of the WSSV load. Statistical analysis indicated that there was no significant difference (P>0.05) between the WSSV load in pond water and shrimp, and there was no relationship between total bacterial load and viral load in the pond water. However, a significant difference (P<0.01) was found between HPV load and L. vannamei and F. chinensis pond water.
( In Kwon Jang ),( Kannan Suriakala ),( Jong Sheek Kim ),( Xian Hong Meng ),( Tae Jin Choi ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.11
A highly sensitive and specific TaqMan real-time PCR was used to quantify hepatopancreatic parvovirus (HPV) type III infections in wild broodstocks and hatcheryreared postlarvae (PL) of Fenneropenaeus chinensis. Totals of 159 and 162 wild brooders from three locations were captured, and 140 and 180 PL were obtained from seven and six commercial hatcheries in 2007 and 2008, respectively. Among the three wild broodstock groups from 2007, only 1 group showed HPV infection and 3.2% of 159 brooders were positive for HPV infection. In 2008, HPV infections were observed from all three wild broodstock groups with 1.93×10(4) copies/mg tissue of pleopods. Of 162 brooders, 26.6% were positive for HPV infection. No PL from the two hatcheries collected in 2007 showed HPV infection, and PL from the rest of the five hatcheries had up to 1.74×10(6) copies/ng of DNA, and PL from three hatcheries showed HPV infections with over 1,000 copies/ng of DNA. The PL from all seven hatcheries collected in 2008 showed up to 2.10×10(5) HPV copies/ng of DNA. PL from two hatcheries showed less than 100 copies/ng of DNA, but PL from the rest of the hatcheries showed HPV infections with over 1,000 copies/ng of DNA. These results show that HPV type III is widely distributed in Korea in addition to previously reported HPV type I, and they can be effectively detected by type-specific real-time PCR.
Jang, In-Kwon,Gopalakannan, Ayyaru,Suriakala, Kannan,Kim, Jong-Sheek,Kim, Bong-Rae,Cho, Yeong-Rok,Meng, Xian-Hong,Seo, Hyeong-Chul The Korean Society of Fisheries and Aquatic Scienc 2008 Fisheries and Aquatic Sciences Vol.11 No.4
Viral diseases are major emerging problems of shrimp that have affected the production, and even complete losses for shrimp farms. In this study, we developed a sensitive TaqMan real-time PCR method to quantify white spot syndrome virus (WSSV) and hepatopancreatic parvovirus (HPV) in the shrimp and pond water in which fleshy shrimp, Fenneropenaeus chinensis, and Pacific white shrimp, Litopenaeus vannamei, are reared. WSSV and HPV in pond seawaters ranged from $1.65{\times}10^3$ to $2.43{\times}10^9$ and from 0 to $4.43{\times}10^5$ copies/L of seawater, respectively. Of 20 ponds analyzed, all pond water and shrimp were positive for WSSv. L. vannamei showed higher susceptibility to WSSV than F chinensis. HPV was detected only in the pond water for F chinensis. In shrimp tissue, however, HPV was found in both species, with 23-times higher infection rate in F chinensis than L. vannamei. The total bacterial counts in the pond water ranged from $2.23{\times}l0^3$ to $1.98{\times}l0^5\;CFU/mL$. The variations in total bacterial count for each pond appeared to correlate to the variations of the WSSV load. Statistical analysis indicated that there was no significant difference (P>0.05) between the WSSV load in pond water and shrimp, and there was no relationship between total bacterial load and viral load in the pond water. However, a significant difference (P<0.01) was found between HPV load and L. vannamei and F chinensis pond water.