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장인권,Ayyaru Gopalakannan,Kannan Suriakala,김종식,김봉래,조영록,Xian-Hong Meng,서형철 한국수산과학회 2008 Fisheries and Aquatic Sciences Vol.11 No.4
Viral diseases are major emerging problems of shrimp that have affected the production, and even complete losses for shrimp farms. In this study, we developed a sensitive TaqMan real-time PCR method to quantify white spot syndrome virus (WSSV) and hepatopancreatic parvovirus (HPV) in the shrimp and pond water in which fleshy shrimp, Fenneropenaeus chinensis, and Pacific white shrimp, Litopenaeus vannamei, are reared. WSSV and HPV in pond seawaters ranged from 1.65×103 to 2.43×109 and from 0 to 4.43×105 copies/L of seawater, respectively. Of 20 ponds analyzed, all pond water and shrimp were positive for WSSV. L. vannamei showed higher susceptibility to WSSV than F. chinensis. HPV was detected only in the pond water for F. chinensis. In shrimp tissue, however, HPV was found in both species, with 23-times higher infection rate in F. chinensis than L. vannamei. The total bacterial counts in the pond water ranged from 2.23×103 to 1.98×105 CFU/mL. The variations in total bacterial count for each pond appeared to correlate to the variations of the WSSV load. Statistical analysis indicated that there was no significant difference (P>0.05) between the WSSV load in pond water and shrimp, and there was no relationship between total bacterial load and viral load in the pond water. However, a significant difference (P<0.01) was found between HPV load and L. vannamei and F. chinensis pond water.
Jang, In-Kwon,Gopalakannan, Ayyaru,Suriakala, Kannan,Kim, Jong-Sheek,Kim, Bong-Rae,Cho, Yeong-Rok,Meng, Xian-Hong,Seo, Hyeong-Chul The Korean Society of Fisheries and Aquatic Scienc 2008 Fisheries and Aquatic Sciences Vol.11 No.4
Viral diseases are major emerging problems of shrimp that have affected the production, and even complete losses for shrimp farms. In this study, we developed a sensitive TaqMan real-time PCR method to quantify white spot syndrome virus (WSSV) and hepatopancreatic parvovirus (HPV) in the shrimp and pond water in which fleshy shrimp, Fenneropenaeus chinensis, and Pacific white shrimp, Litopenaeus vannamei, are reared. WSSV and HPV in pond seawaters ranged from $1.65{\times}10^3$ to $2.43{\times}10^9$ and from 0 to $4.43{\times}10^5$ copies/L of seawater, respectively. Of 20 ponds analyzed, all pond water and shrimp were positive for WSSv. L. vannamei showed higher susceptibility to WSSV than F chinensis. HPV was detected only in the pond water for F chinensis. In shrimp tissue, however, HPV was found in both species, with 23-times higher infection rate in F chinensis than L. vannamei. The total bacterial counts in the pond water ranged from $2.23{\times}l0^3$ to $1.98{\times}l0^5\;CFU/mL$. The variations in total bacterial count for each pond appeared to correlate to the variations of the WSSV load. Statistical analysis indicated that there was no significant difference (P>0.05) between the WSSV load in pond water and shrimp, and there was no relationship between total bacterial load and viral load in the pond water. However, a significant difference (P<0.01) was found between HPV load and L. vannamei and F chinensis pond water.
사육수 비교환 방식에 의한 흰다리새우의 고밀도 사육 I. 후기유생(postlarva)의 실내 중간육성
장인권,김종식,조국진,서형철,조영록,Ayyaru Gopalakannan,김봉래 한국양식학회 2008 韓國養殖學會誌 Vol.21 No.4
Farming of the fleshy shrimp Fenneropenaeus chinensis which is a major cultured species in the west coast of South Korea, has been suffered from mass mortality due to disease epizootics including viruses. Since the Pacific white shrimp Litopenaeus vannamei was introduced to Korea in 2003, farming of this species has rapidly increased for years, occupying 62.5% of total cultured shrimp production in 2007. However the studies on L. vannamei culture methods for shrimp farming situations in Korea are very limited. Nursery culture of shrimp larvae has some advantages including increased survival, improved feed efficiencies, enhanced growth performance and reduced grow-out period. In this study, L. vannamei postlarvae (PL3-PL10) with a density of 3,750-9,090/m3 were cultured in four raceways under limited water exchange condition for 35 days. Survival was the highest (93.6%) in tank stocked with 4,090/m3 and was the lowest in tank with 9,090/m3(58.1%). Mean body weight at harvest ranged from 0.071 to 0.108 g, and FCR was 0.59-0.70 in all tanks. Concentration of total ammonia nitrogen was increased up to 20 ppm on day 10 in all tanks and thereafter gradually decreased by the third week of culture. Nitrite-nitrogen was rapidly increased from the third week, representing bio-floc condition by developed nitrifying bacterial community. Of the present nursery system some modification of structure and consideration for commercial scale are needed in order to be implemented to shrimp farmers.