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Shim, Doo-Bo,Park, Eun-Sil,Sim, Gyu-Jin,Lee, Ji-Young,Kang, Ju-Hwan,Yoo, Hyun-Joo,Choi, Yeon-Jin,Lee, Young-Mee,Lee, Sang-Yeol,Kim, Min-Gab,Kang, Da-Won,Jung, Eun-Jung,Kang, Kee-Ryeon The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
Eukaryotic translation initiation factor 5A (eIF5A) is the only hypusine-containing protein, which is formed by deoxyhypusine synthase (DHS) and deoxyhypusine hydroxylase (DOHH). DOHH is a novel metalloenzyme with HEAT [named for human huntingtin (H), elongation factor 3 (E), a subunit of protein phosphatase 2A (A), and the target of rapamycin (T)]-repeat motifs. Inspite of much progress in determining the roles of iron-containing DOHH holoenzyme as an eIF5A hydroxylase, little is known about iron-free apoenzyme of DOHH under certain stress conditions. For this purpose, we compared cell growth in two yeast strains subjected to oxidative damage. Thus, the existence of more viable cells in the Saccharomyces cerevisiae BY4743 (parental yeast) strain than in the DOHH- strain after $H_2O_2$ treatment indicates the importance of DOHH in protecting yeast cells against oxidative stress. To identify endogenous target proteins influenced by DOHH under oxidative damage, proteomic analysis was applied to the two yeast strains. Of these proteins, the oxidized form of peroxiredoxin I (PrxI) was concomitantly up-regulated in both strains under $H_2O_2$ treatment. Two-dimensional electrophoresis (DE) followed by immunoblot analysis shows that the recovery of the oxidized PrxI to the reduced enzyme under $H_2O_2$ treatment was found to be much slower in the DOHH- strain than in the parental strain. Based on the results, we discovered a possible interaction between DOHH and PrxI by immunoprecipitation/immunoblotting in yeast under oxidative stress. Taken together, these results suggest that DOHH might be a candidate protein for protection of yeast cells against oxidative stress in conjunction with PrxI.
Kang-Moon Song,Doo Yong Chung,Min Ji Choi,Kalyan Ghatak,Nguyen Nhat Minh,Anita Limanjaya,Mi-Hye Kwon,옥지연,Guo Nan Yin,Dae-Kee Kim,Ji-Kan Ryu,Jun-Kyu Suh 대한남성과학회 2020 The World Journal of Men's Health Vol.38 No.4
Purpose: To examine the therapeutic effect of Vactosertib, a small molecule inhibitor of transforming growth factor-β (TGF-β) type I receptor (activin receptor-like kinase-5, ALK5), in an experimental model of Peyronie’s disease (PD) and determining anti-fibrotic mechanisms of Vactosertib in primary fibroblasts derived from human PD plaques. Materials and Methods: Male rats were randomly divided into three groups (n=6 per group); control rats without treatment; PD rats receiving vehicle; and PD rats receiving Vactosertib (10 mg/kg). PD-like plaques were induced by administering 100 μL of each of human fibrin and thrombin solutions into the tunica albuginea on days 0 and 5. Vactosertib was given orally five times a week for 2 weeks. On day 30, we performed electrical stimulation of the cavernous nerve to measure erectile function, and the penis was obtained for histological examination. Fibroblasts isolated from human PD plaques were used to determine the anti-fibrotic effects of Vactosertib in vitro. Results: Vactosertib induced significant regression of fibrotic plaques in PD rats in vivo through reduced infiltration of inflammatory cells and reduced expression of phospho-Smad2, which recovered erectile function. Vactosertib also abrogated TGF- β1-induced enhancement of extracellular matrix protein production and hydroxyproline content in PD fibroblasts in vitro by hindering the TGF-β1-induced Smad2/3 phosphorylation and nuclear translocation, and fibroblast-to-myofibroblast transdifferentiation. Conclusions: In view of the critical role of TGF-β and the Smad pathway in the pathogenesis of PD, inhibition of this pathway with an ALK5 inhibitor may represent a novel, targeted therapy for PD.
한국 남해에 출현하는 먹장어 Eptatretus burgeri 의 재생산 특성
김두남 ( Doo-nam Kim ),황강석 ( Kang-seok Hwang ),차형기 ( Hyung-kee Cha ),박준수 ( Jun-su Park ),김정년 ( Jung-nyun Kim ),문성용 ( Seong-yong Moon ),이정훈 ( Jeong-hoon Lee ) 한국어업기술학회 2018 수산해양기술연구 Vol.54 No.2
The reproduction characteristics of hagfish Eptatretus burgeri were examined using individuals caught in the South Sea of Korea. The spawning season and size at minimum sexual maturity of this species were characterized based on a gonad-somatic index (GSI) and monthly variation egg size (long axis). From monthly variation of GSI, the spawning season was estimated to be from August to September. Developing eggs larger than 10 mm were found in March, and the largest egg size was found in July. The first spawning length was 34.2 cm TL. Batch fecundity ranged from 13 to 117 eggs for hagfish sized from 34.2 cm TL to 77.0 cm TL, respectively, and increased linearly with total length.