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Shaotian Chen,Jing Gong,Kaiyun Guan,Zhekun Zhou 한국식물학회 2010 Journal of Plant Biology Vol.53 No.6
Incarvillea is a genuswith considerable ornamental and medicinal value, yet many of its 16 species are threatened in the wild and two (Incarvillea forrestii and Incarvillea altissima) may be extinct. An extensive field survey of the Chinese species has shown that logging is the greatest threat to woodland margin species, while overgrazing by cattle endangers species from alpine meadows. To aid the setting of conservation priorities, we used phylogenetic weighting of species, based on the molecular phylogeny of both nuclear (nrITS) and chloroplast (trnL-F region) gene regions. We estimated molecular diversity with measures for genetic diversity (GD) and phylogenetic diversity (PD). PD/GD values highlighted the importance of Incarvillea sinensis and two Central Asian species, Incarvillea semiretschenskia and Incarvillea olgae from Kazakstan and Kirghizia, for which little is known. This combination of phylogenetic and complementarity analyses focused on the provinces of Sichuan, Yunnan, and Qinghai, with Yunnan having the only adequately protected areas for that genus.
Takagi, Hiroki,Sugawara, Shintaro,Saito, Tomoka,Tasaki, Haruka,Yuanxue, Lu,Kaiyun, Guan,Han, Dong-Sheng,Godo, Toshinari,Nakano, Masaru The Korean Society of Plant Biotechnology 2011 Plant biotechnology reports Vol.5 No.2
The gesneriaceous perennial plant Titanotrichum oldhamii has beautiful foliage and attractive bright yellow flowers. However, breeding of T. oldhamii by conventional sexual hybridization may be difficult because sexual reproduction of this species is very rare. In the present study, plant regeneration systems via both direct and indirect formation of adventitious shoots from leaf explants were established as the first step toward breeding T. oldhamii by using biotechnological techniques. Adventitious shoots were formed efficiently on medium containing $0.1mg\;l^{-1}$ benzyladenine. Histological observation showed that shoot formation on this medium occurred directly from leaf epidermal cells without callus formation. On the other hand, leaf explants formed calluses on medium containing $0.1mg\;l^{-1}$ 2,4-dichlorophenoxyacetic acid. The calluses could be maintained by monthly subculturing to fresh medium of the same composition. When the calluses were transferred to plant growth regulator-free medium, they formed adventitious shoots. Directly and indirectly formed shoots rooted well on medium containing $0.1mg\;l^{-1}$ indole-3-butyric acid. Plantlets thus obtained were successfully acclimatized and grew vigorously in the greenhouse. Flow cytometry analysis indicated that no variation in the ploidy level was observed in plants regenerated via direct shoot formation, whereas chromosome doubling occurred in several plants regenerated via indirect shoot formation. Regenerated plants with the same ploidy level as the mother plants showed almost the same phenotype as the mother plants, whereas chromosome-doubled plants showed apparent morphological alterations: they had small and crispate flowers, and round and deep green leaves.