http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Phosphate-Responsive Promoter of a Pichia pastoris Sodium Phosphate Symporter
Ahn, Jungoh,Hong, Jiyeon,Park, Myongsoo,Lee, Hyeokweon,Lee, Eungyo,Kim, Chunsuk,Lee, Joohwan,Choi, Eui-sung,Jung, Joon-ki,Lee, Hongweon American Society for Microbiology 2009 Applied and environmental microbiology Vol.75 No.11
<B>ABSTRACT</B><P>To develop a functional phosphate-regulated promoter in <I>Pichia pastoris</I>, a phosphate-responsive gene, <I>PHO89</I>, which encodes a putative sodium (Na<SUP>+</SUP>)-coupled phosphate symporter, was isolated. Sequencing analyses revealed a 1,731-bp open reading frame encoding a 576-amino-acid polypeptide with 12 putative transmembrane domains. The properties of the <I>PHO89</I> promoter (<I>PPHO89</I>) were investigated using a bacterial lipase gene as a reporter in 5-liter jar fermentation experiments. <I>PPHO89</I> was tightly regulated by phosphate and was highly activated when the cells were grown in a phosphate-limited external environment. Compared to translation elongation factor 1α and the glyceraldehyde-3-phosphate dehydrogenase promoter, <I>PPHO89</I> exhibited strong transcriptional activity with higher specific productivity (amount of lipase produced/cell/h). Furthermore, a cost-effective and simple <I>PPHO89</I>-based fermentation process was developed for industrial application. These results demonstrate the potential for efficient use of <I>PPHO89</I> for controlled production of recombinant proteins in <I>P. pastoris</I>.</P>
Ahn, Jungoh,Chung, Bevan K S,Lee, Dong-Yup,Park, Myongsoo,Karimi, Iftekhar A,Jung, Joon-Ki,Lee, Hongweon Published by Elsevier/North Holland on behalf of t 2011 FEMS microbiology letters Vol.324 No.1
<P>We explored the physiological and metabolic effects of different carbon sources (glucose, fructose, and glucose/fructose mixture) in phosphoglucose isomerase (pgi) knockout Escherichia coli mutant producing shikimic acid (SA). It was observed that the pgi(-) mutant grown on glucose exhibited significantly lower cell growth compared with the pgi(+) strain and its mixed glucose/fructose fermentation grew well. Interestingly, when fructose was used as a carbon source, the pgi(-) mutant showed the enhanced SA production compared with the pgi(+) strain. In silico analysis of a genome-scale E. coli model was then conducted to characterize the cellular metabolism and quantify NAPDH regeneration, which allowed us to understand such experimentally observed attenuated cell growth and enhanced SA production in glucose- and fructose-consuming pgi(-) mutant, respectively with respect to cofactor regeneration.</P>
Hong, Minhee,Ahn, Jungoh,Yoo, Sungsik,Hong, Jiyeon,Lee, Eungyu,Yoon, Injoong,Jung, Joon-ki,Lee, Hongweon Elsevier 2011 Veterinary microbiology Vol.148 No.1
<P><B>Abstract</B></P><P><I>Haemophilus parasuis</I> causes contagious porcine Glässer's disease, which is occurring worldwide and leads to severe losses in the pig industry. To identify novel antigen candidates against this disease, 22 surface-exposed or secreted proteins were selected from the annotated <I>H. parasuis</I> genome by reverse vaccinology strategy. Expression of these proteins in <I>Escherichia coli</I> was attempted. Immunogenicity of the expressed candidates was assessed using Western blot analysis with mouse-derived antiserum prepared with whole bacteria of <I>H. parasuis</I> serovar 4 or 5. Three ABC-type transporters (OppA, YfeA and PlpA) and 1 curli protein assembly (CsgG) were identified as potent immunogenic proteins. The proteins show cross-reactions when tested with sera raised against serovars 4 and 5 of <I>H. parasuis</I>.</P>
여인석 ( In-seok Yeo ),전우영 ( Wooyoung Jeon ),안정오 ( Jungoh Ahn ) 한국공업화학회 2023 공업화학전망 Vol.26 No.2
2050년 탄소중립(Net zero)을 맞추기 위해, 최근 국내 석유화학 기업들은 재생 가능한 원료로 만들어지는 리사이클·바이오 플라스틱(Recyclable·bio-based plastic) 등을 미래 사업으로 선정하여 대규모 투자와 함께 역량을 쏟고 있다. 이에, 기존 석유계 플라스틱을 리사이클·바이오 플라스틱으로 대체하기 위해, 재순환·재생 가능한 원료로부터 친환경 공법인 생물공정기반으로 플라스틱 단량체들을 생산하는 기술들이 국내·외에서 개발되고 있다. 재생 가능한 자원인 바이오매스 중 당류 및 지질 등이 주로 생물공정 원료로 사용되고 있지만, 지질은 당류에 비해 2.25배 이상의 높은 에너지 밀도를 가지고 있으며, 기존 석유화학 소재와 유사한 탄화수소 구조를 가지고 있어, 석유화학소재로 전환하는데 유리하다. 본 기고문에서는 기존 석유원료와 환경오염 유발적인 화학공정으로 생산된 화학소재를 재생 가능한 원료로부터 친환경 생물공정을 통해 생산하는 기술을 개발하는 산업바이오 분야에 대해서 설명하고, 자동차 경량화에 핵심 소재 중에 하나인 엔지니어링 플라스틱인 폴리아마이드를 중심으로 식물유로부터 단량체 생산을 위한 미생물 반응에 대해서 살펴보고자 한다.
( Hun-suk Song ),( Jong-min Jeon ),( Yong Keun Choi ),( Jun-young Kim ),( Wooseong Kim ),( Jeong-jun Yoon ),( Kyungmoon Park ),( Jungoh Ahn ),( Hongweon Lee ),( Yung-hun Yang ) 한국미생물생명공학회(구 한국산업미생물학회) 2017 Journal of microbiology and biotechnology Vol.27 No.12
Lignocellulose is now a promising raw material for biofuel production. However, the lignin complex and crystalline cellulose require pretreatment steps for breakdown of the crystalline structure of cellulose for the generation of fermentable sugars. Moreover, several fermentation inhibitors are generated with sugar compounds, majorly furfural. The mitigation of these inhibitors is required for the further fermentation steps to proceed. Amino acids were investigated on furfural-induced growth inhibition in E. coli producing isobutanol. Glycine and serine were the most effective compounds against furfural. In minimal media, glycine conferred tolerance against furfural. From the IC<sub>50</sub> value for inhibitors in the production media, only glycine could alleviate growth arrest for furfural, where 6 mM glycine addition led to a slight increase in growth rate and isobutanol production from 2.6 to 2.8 g/l under furfural stress. Overexpression of glycine pathway genes did not lead to alleviation. However, addition of glycine to engineered strains blocked the growth arrest and increased the isobutanol production about 2.3-fold.