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Candida albicans mannoproteins 자극에 의한 저출생체중아와 만삭아 제대혈액 단핵구의 TNF-α 생산
백설향,김우경,정초록 동국대학교 경주대학 1998 東國論集 Vol.17 No.2
Background : Opportunistic fungal infections of low birth weight infants(LBWI) remains a significant cause of morbidity and mortality. Concerning infantile infections, Tumor Necrosis Factor(TNF)-α production by C.albicans may be important to consider in clinical circumstances relating to candidaemia and/or candida septicemia. TNF-α/cachentin that has multiple immunologic and inflammatory functions is produced by monocytes and macrophages, and its sunnormal production by term infants(TI) and LBWI may be related to the their immunosusceptibilities. Methods : This study examined the production of TNF-α by cord blood monocytes from LBWI and TI stimulated with manoproteins from Candida albicans(MCA). In briefly, supernatants of monocytes cultures with MCA possessed TNF-α-mediated cytotoxicity against the L929 fibroblasts, as measured in the modified MTT colorimetric assay. Data were analyzed by Student t test or analysis of variance(Scheffe's post-hoc test). Results : 1) TNF-α production was no dectectable with the supernatants of monocyes from either LBWI or TI at 6 hr after MCA stumulated. 2) TNF-α production by LBWI was significantly less than that from monocytes of either TI or adult group at 18 hr(p=.000). 3) After 18 hr incubation with MCA, TNF-α production by monocytes of TI and LBWI reached at highest level, but significantly less than that of adult group at the same time(p=.000). 4) For the both infants groups, TNF-α production was significantly decreased after 24 hr(p=.000), compared with its production by adult group was continuously elevated, but without significance (p >.05). Conclusions : These results suggested that MCA were strong inducer of the TNF-α and given the multifactorial biologic activities of TNF-α, the decreased secretion of TNF-α from LBWI and TI may be significant in describing mechanisms for the increased susceptibility of them to the antigens such as MCA. And, these data suggest an important involvement of TNF-α in the infectious immunity induced by MCA.
Cho-Rok Jung,Min-Jung Park,Moon-Soo Heo 한국미생물학회 2005 The journal of microbiology Vol.43 No.5
Sera from rabbits were infected with Vibrio vulnificus containing an antibody against major outer membrane protein (MOMP). MOMP of V. vulnificus ATCC 27562 were isolated and purified by Sarkosyl and TritonX-100 dual treatment. Molecular size of MOMP was identified as 36-kDa on 13% SDS-PAGE. The sequence of the first 26 amino acid residues from the N-terminal end of the protein is AELYNQDGTSLDMGGRAEARLSMKDG, which is a perfect match with OmpU of V. vulnificus CMCP6 and YJ016. MOMP specific IgM and IgG were investigated in groups of mice. The group of mice immunized with MOMP and Alum showed higher levels of IgG2b than the group immunized with only MOMP. Vaccination with MOMP resulted in protective antibodies in the mouse infection experiment.
Jung Cho-Rok,Park Min-Jung,Heo Moon-Soo The Microbiological Society of Korea 2005 The journal of microbiology Vol.43 No.5
Sera from rabbits were infected with Vibrio vulnificus containing an antibody against major outer membrane protein (MOMP). MOMP of V. vulnificus ATCC 27562 were isolated and purified by Sarkosyl and TritonX-100 dual treatment. Molecular size of MOMP was identified as 36-kDa on $13\%$ SDS-PAGE. The sequence of the first 26 amino acid residues from the N-terminal end of the protein is AELYNQDGTSLDMGGRAEARLSMKDG, which is a perfect match with OmpU of V. vulnificus CMCP6 and YJ016. MOMP specific IgM and IgG were investigated in groups of mice. The group of mice immunized with MOMP and Alum showed higher levels of IgG2b than the group immunized with only MOMP. Vaccination with MOMP resulted in protective antibodies in the mouse infection experiment.
Jung, Cho-Rok,Yoo, Jinsang,Jang, Ye Jin,Kim, Sangsoo,Chu, In-Sun,Yeom, Young Il,Choi, Jong Young,Im, Dong-Soo Wiley Subscription Services, Inc., A Wiley Company 2006 Hepatology Vol.43 No.5
<P>The pituitary tumor transforming (PTTG) gene family comprises PTTG1, 2, and 3. Forced expression of PTTG1 (securin) induces cellular transformation and promotes tumor development in animal models. PTTG1 is overexpressed in various human cancers. However, the expression and pathogenic implications of the PTTG gene family in hepatocellular carcinoma are largely unknown. Gene silencing using short interfering RNA (siRNA) has become an efficient means to study the functions of genes and has been increasingly used for cancer gene therapy approaches. We report that PTTG1, but not PTTG2 and 3, was highly and frequently expressed in liver cancer tissues from patients and highly in SH-J1, SK-Hep1, and Huh-7 hepatoma cell lines. Adenoviral vector encoding siRNA against PTTG1 (Ad.PTTG1-siRNA) depleted PTTG1 specifically and efficiently in SH-J1 hepatoma cells, which resulted in activation of p53 that led to increased p21 expression and induction of apoptosis. The depletion of PTTG1 in HCT116 colorectal cancer cells exhibited a cytotoxic effect in a p53-dependent manner. Ad.PTTG1-siRNA-mediated cytotoxic effect was dependent on expression levels of PTTG1 and p53 in hepatoma cell lines. Huh-7 hepatoma cells, once transduced with Ad.PTTG1-siRNA, displayed markedly attenuated growth potential in nude mice. Intra-tumor delivery of Ad.PTTG1-siRNA led to significant inhibition of tumor growth in SH-J1 tumor xenograft established in nude mice. In conclusion, PTTG1 overexpressed in hepatoma cell lines negatively regulates the ability of p53 to induce apoptosis. PTTG1 gene silencing using siRNA may be an effective modality to treat liver cancer, in which PTTG1 is abundantly expressed. (HEPATOLOGY 2006;43:1042–1052.)</P>
Case Report : Linear Lichen Sclerosus along the Blaschko`s Line of the Face
( Cho Rok Kim ),( Kyu Dong Jung ),( Hyun Je Kim ),( Mi Young Jung ),( Ji Yeon Byun ),( Dong Youn Lee ),( Joo Heung Lee ),( Jun Mo Yang ),( Eil Soo Lee ) 대한피부과학회 2011 Annals of Dermatology Vol.23 No.2
Lichen sclerosus et atrophicus (LSA) is an inflammatory disease that primarily causes anogenital lesion in middle aged women. We present here a case of facial LSA with an asymptomatic, well-demarcated, whitish to bluish, atrophic patch in a linear pattern on the forehead of a 48-year-old woman. This case showed an atypical clinical presentation and it mimicked en coup de sabre, but the histopathologic results confirmed the diagnosis of LSA. (Ann Dermatol 23(2) 222∼224, 2011)
Global gene expression profile of Orientia tsutsugamushi
Cho, Bon-A,Cho, Nam-Hyuk,Min, Chan-Ki,Kim, Se-Yoon,Yang, Jae-Seong,Lee, Jung Rok,Jung, Jin Woo,Lee, Won-Chul,Kim, Kijeong,Lee, Mi-Kyung,Kim, Sanguk,Kim, Kwang Pyo,Seong, Seung-Yong,Choi, Myung-Sik,Kim WILEY-VCH Verlag 2010 Proteomics Vol.10 No.8
<P>Orientia tsutsugamushi, an obligate intracellular bacterium, is the causative agent of Scrub typhus. The control mechanisms for bacterial gene expression are largely unknown. Here, the global gene expression of O. tsutsugamushi within eukaryotic cells was examined using a microarray and proteomic approaches for the first time. These approaches identified 643 genes, corresponding to approximately 30% of the genes encoded in the genome. The majority of expressed genes belonged to several functional categories including protein translation, protein processing/secretion, and replication/repair. We also searched the conserved sequence blocks (CSBs) in the O. tsutsugamushi genome which is unique in that up to 40% of its genome consists of dispersed repeated sequences. Although extensive shuffling of genomic sequences was observed between two different strains, 204 CSBs, covering 48% of the genome, were identified. When combining the data of CSBs and global gene expression, the CSBs correlates well with the location of expressed genes, suggesting the functional conservation between gene expression and genomic location. Finally, we compared the gene expression of the bacteria-infected fibroblasts and macrophages using microarray analysis. Some major changes were the downregulation of genes involved in translation, protein processing and secretion, which correlated with the reduction in bacterial translation rates and growth within macrophages.</P>