The effect of neuropeptides on secretion of Interleukin-8(IL-8)

Kim, Kyung-Jun,Park, Sang-Hyuk,Choi, Kyoung-Kyu,Park, Sang-Jin 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.3

본 연구는 치수조직, 치은, 치주인대로부터 배양된 조직을 SP (Substance P)로 4시간, SP, CGRP (Calcitonin Gene-related Peptide), Tumor necrosis factor-α (TNF-α)로 8시간 자극 후 RNase Protection Assay를 시행하고, IL-8의 분비량을 측정해 다음 결과를 얻었다. 1. IL-8 mRNA는 모든세포에서 발현됐다. 2. IL-8 mRNA 발현은 SP (10^(-5)M)와 SP (10^(-8)M)로 4시간 자극 시 증가되지 않았다. 3. IL-8 mRNA 발현은 SP (10^(-4)M)와 CGRP (10^(-6)M)로 8시간 자극 시 증가되지 않았다. 4. TNF-α (2 ng/㎖) 자극 시, IL-8 mRNA 발현이 증가됐다. 5. 치은 세포를 CGRP (10^(-6)M)로 8시간 자극 시, IL-8 분비량이 증가했다 (p < 0.05). 6. 치주인대 세포를 SP (10^(-4)M)로 8시간 자극 시 IL-8 분비량이 증가했다 (p < 0.05). We investigated the secretion of Interleukin-8 (IL-8) from ginviva and periodontal ligament stimulated with Substance P (SP) and Calcitonin Gene-related Peptide (CGRP). Gingiva (GF), periodontal ligament (PDLF) and pulp (PF) tissues were collected from extracted instact 3rd molars. Cultured cells were stimulated with different concentrations of SP for 4 hrs, and stimulated with SP, CGRP and Tumor Necrosis Factor-α (TNF-α) for 8 hrs. Then RNase Protection Assay was carried out. ELISA was performed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using supernatants of stimulated cells for quantitative analysis of IL-8. Results were assessed using student t-test with significance of P < 0.05. According to this study, the results were as follows: 1. IL-8 mRNA was detected in all type of cells studied (PF, GF and PDLF). 2. IL-8 mRNA expression was not increased after stimulating 4 hrs with SP (10^(-5)M) and SP (10^(-8)M) compared with Mock stimulation in all type of cells studied. 3. IL-8 mRNA expression was not increased after stimulating 8 hrs with SP (10^(-4)M) and CGRP (10^(-6)M) compared with Mock stimulation in all type of cells studied. 4. TNF-α (2 ng/㎖) increased the expression of IL-8 mRNA in all kind of cells studied. 5. The secretion of IL-8 from GF was increased 8 hrs after the stimulation with CGRP (10^(-6)M) (p < 0.05). 6. The secretion of IL-8 from PDLF was increased 8 hrs after the stimulation with SP (10^(-4)M) (p < 0.05). Calcitonin Gene-related Peptide (CGRP) increased Interleukin-8 (IL-8) which plays an important role in chemotaxis of neutrophil in Calcitonin Gene-related Peptide (CGRP) gingival tissue, whereas Substance P increased the secretion of IL-8 from periodontal ligament.

1960년대 "남조선해방전략당"의 형성과 성격

전명혁 ( Myung Hyuk Jun ) 수선사학회 2016 史林 Vol.0 No.56

This paper was written for the purpose to examine the making and characteristic of ‘Liberation and Strategy Party in Southern Korea Case’ in 1960s. The case was published as ‘Unified Revolutionary Party case’ by the Korean Central Intelligence Agency, Aug., 1968. So far in the memories of people, there are engraved as like ‘Unified Revolutionary Party case’. But people who involved in this case is basically who were the activists of the labor movement in Daegu, Busan and elsewhere from the end of the 1950s to the early 1960s. At the period of 4.19 they organized ‘a Labor Union League in Daegu’ which is democratic and voluntary labor union. But their activity was stopped with 5.16 Coup. However, their activity began with again Kwon Jai Hyuk’s presentation in seminar of the Democratic Socialist Dongihoe in September 1963, with Lee IL Jai’s activity of organizer. I whether these groups has had its own program is not yet known. However, they had a recognition of "needs its own and voluntary efforts to resolve the south Korea’s contradiction in the working class themselves” under the basic strategy of ‘the struggle for anti imperialism, anti feudalism and anti dictatorship’. Therefore, this group anticipate to build their own ‘vanguard organization’ based on the working class, the labor movement.

소아 심장 CT 검사시 Dual Source CT의 High pitch mode를 이용한 선량감소에 관한 연구

이예나(Ya Na Lee),강헌효(Heon Hyo Kang),손성실(Sung Sil Son),박찬혁(Chan Hyuk Park),현진경(Jin Kyung Hyun),신명호(Myung Ho Shin),김준혁(Jun Hyuk Kim),강동원(Dong Won Kang) 대한CT영상기술학회 2012 대한CT영상기술학회지 Vol.14 No.1

I. Purpose This study examines if accurate images can be realized with less dose than sequential mode, one of the existing methods applying DSCT’s high-pitch mode in pediatric cardiography. II. Materials and Methods For 20 patients who came to our hospital from Jan. 1 to Jun. 30, 2011, a test was carried out using MDCT 128 channel dual source; Somatom Definition Flash (Siemens Medical System: Germany). As the test method, the veins of the lower limbs of the patients were secured and contrast medium of 7ml was injected at 0.3ml/s anda CT scan was carried out about 36 s. later. Af first, sequential mode was used, and then high-pitch mode was followed as follow-up examination. The DLP (Dose Length Product) provided by the equipment and effective dose were compared for the assessment of the dose exposed to the patients, after the CT scan. The effective does was calculated by multiplying children’s chest index: 0.021 of the effective dose rate of EUR16262 (European Guide) to DLP value. The images scaned in high-pitch method were compared and assessed quantitatively by a heart decoding specialist and 2 radiologists if the typical diseases of pediatric cardiacs such as coarctation of aorta, atresia of the pulmonary artery and Tetralogy of Fallot can be assessed. III. Results When it was compared to sequential mode, one of the existing cardiography, the effective dose exposed to the patient had decrease by 32% in high-pitch mode. As a result of a comparison with imaging assessment, it was also evaluated as a better imaging than sequential mode IV. Conclusion Pediatric Cardiography using high pitch in Dual source MDCT showed superior effects on the reduction of exposed does than sequential mode, which is considered a useful testing method. 목적 본 연구에서는 소아 심장 검사 시 DSCT의 High-pitch mode를 적용하여 기존의 검사방법 중 하나인 Sequential mode보다 적은 선량으로도 정확한 영상을 구현할 수 있는지 알아본다. 대상 및 방법 2011년 1월 1일부터 6월 30일까지 본원에 내원한 20명의 환자를 대상으로 하여 MDCT 128 channel dual source; Somatom Definition Flash(Siemens medical system: Germany) 장비를 사용하여 검사를 시행하였다. 검사방법은 환자의 하지에 정맥을 확보하고 조영제를 0.3 ml/s로 7ml를 주입한 후 36초 후에 CT검사를 하였다. 처음에는 Sequential mode로 검사한 후 추적 검사할 땐 High-pitch mode로 검사한다. 환자가 받은 피폭선량의 평가는 선량평가는 CT검사 후 장비에서 제공하는 DLP(dose length product)와 유효선량(effective dose)를 비교하였다. 유효선량은 DLP 값에 EUR16262(European Guide) 유효선량비 중 소아 흉부 지수 0,021을 곱하여 계산하였다. 검사한 영상을 심장 판독 전문의 1명과 방사선사 2명이 high-pitch mode로 검사한 영상을 소와 심장의 대표적 질환인 대동맥협착증, 폐동맥 폐쇄증, 팔로씨 사증을 평가할 수 있는지 비교하여 정성적으로 평가하였다. 결과 기존의 심장 검사방법 중 하나인 Sequential mode와 비교했을 때 High pitch mode로 검사했을 때에 32%의 선량 감소 효과가 있었다. 또한 영상 평가 비교 결과 Sequential mode 보다 좋은 영상으로 평가되었다. 결론 Dual source MDCT로 소아심장 검사를 할 때 high pitch mode를 이용하면 sequential mode로 검사했을 때보다 환아가 받는 피폭선량은 낮고 더 우수한 영상을 획득하여 심장질환을 진단하는데 유용한 검사법으로 사료된다.

북한의 문화유산 인식과 정책

김준혁 中央大學校 民族發展硏究院 2002 民族發展硏究 Vol.- No.6

화학물질 누출에 의한 대량재해 : Methyl Isocyanate 누출을 중심으로

양혁준,최중명,유동준 한국응급구조학회 1999 한국응급구조학회지 Vol.3 No.1

Background: We are in the edge of some human made disasters such as hazardous materials and air pollution, for example, the world news reported that the city of Bhopal, India had serious victims related with a leaking out of the chemical materials, Methyl Isocyanate and many people in India were killed by. These situations many people who live in this world are world are worrying about are not others, but just ours and people consider about that kind of the disasters are the possible situation to happen to all the people. Therefore, we performed this basic study to recognize the risk of Methyl Isocyanate leak accident and to prepare local disaster plan with EMS system. Method : Trace 8.0, a simulation software made by the U.S. company Safer System was used as a tool to estimate the diffusion distance, area and its victims at the concentrations of 0.02ppm, 0.2ppm 5ppm respectively for an assumed B-city of 2 hundred thousands population count in which was presumed 500kg Methyl Isocyanate gas to leak out. Results : 1. During 1 hour, maximum diffusion distances of 0.02ppm 0.2ppm and 5ppm were 5.41km, 1.61km and 0.29km respectively on the plume impact. 2. Maximum population counts influenced by Methyl Isocyanate gas at the concentrations of 0.02ppm 0.2ppm and 5ppm were 40838, 4346 and 222 on the plume impact, while those were 138238, 17261 and 1588 on the vulnerability impact, respectively. 3. Therefore, 17261 persons must put on respiratory device and 138283 persons must be evacuated to safety place within 1 hour. Conclusions : Only small amount leak of Methyl Isocyanate may cause tremendous chemical disaster in urban area so its disaster plan must be prepared with an accident simulation program and Material Safety Data Sheets(MSDS). Especially, nearby emergency center of an industrial complex must have a strong position about preparation of chemical disaster plan and perform a disaster dill of hazardous material accident annually.

ADSL망에서 가입자 수에 따른 지연 시간 분석

이준혁,홍석원 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

Asymmetric Digital Subscriber Line(ADLS) is considered as one of the promising technologies to provide high-speed Internet access to subscribers. The speed that subscribers can achieve in the ADSL access network varies depending on the various factors. One of the factors is the dimensioning of the access network which is determined by how many users are connected to one DSLAM. In this paper we describe the architecture of ADSL access network and network constituents, and explain two connection models and related protocols for Internet service provisioning. Then we establishes the simulation model where each subscriber connects to a remote server by HTTP connection and download a page on each request. In this paper we analyze the delay and link utilization depending on the number of subscribers, download file sizes, and server capacities. We use OPNET for simulation.

DSP Processor(TMS320C32)를 이용한 화자인증 보안시스템의 구현

함영준,권혁재,최수영,정익주 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.B

The speech includes various kinds of information : language information, speaker's information, affectivity, hygienic condition, utterance environment etc. when a person communicates with others. All technologies to utilize in real life processing this speech are called the speech technology. The speech technology contains speaker's information that among them and it includes a speech which is known as a speaker recognition. DTW(Dynamic Time Warping) is the speaker recognition technology that seeks the pattern of standard speech signal and the similarity degree in an inputted speech signal using dynamic programming. In this study, using TMS320C32 DSP processor, we are to embody this DTW and to construct a security system.

BaTiO₃PTC 소자와 진공 열증착된 Al 전극의 접촉 특성

권혁주,전본근,이재성,이용수,이용현 경북대학교 센서기술연구소 1992 센서技術學術大會論文集 Vol.3 No.1

용융환원로 배기조성을 이용한 철광석의 예비환원 속도론

이준혁,김성수 嶺南大學校 工業技術硏究所 1995 工業技術硏究所論文集 Vol.23 No.1

Using CO-H₂O and CO-CO₂-H₂O gas mixtures which are a portion of exhaust gas from Bath Smelter, the effect of H₂O partial pressure on the prereduction kinetics of Iron oxide was investigated. H₂O partial pressure in the gas mixture was allowed to change from 0%, 5%, 10% to 15%. Reduction experiments were made at the temperatures of 800, 900, 1000℃ respectively for acid and basic pellets. When the acid pellet was reduced by CO-CO₂-H₂O mixing gas, the incubation period appeared at approximate 27% reduction degree at which Iron oxide was thought to remain as wustite. As the reduction temperature decreased, the incubation period was longer, and because the poisoning effect of H₂O took place at the reduction step of FeO to Fe, it could be said that the total reduction proceeded by the next two steps; Fe₂O₃ to FeO, and FeO to Fe. For the reduction of basic pellet, the poisoning effect of H₂O was remarkable at the reduction stage of Fe₂O₃ to FeO, and reaction rate became faster around 35% reduction degree than that of initial stage. From the experimental result, it was found that the poisoning effect of H₂O for the basic pellet appeared at the reduction step of Fe₂O₃ to FeO, but this effect appeared at the reduction step of FeO to Fe for the acid pellet. The kinetic data were satisfactorily fitted to homogeneous model from which the apparent rate constant(k) was calculated. With the apparent rate constant and following equation, H₂O poisoning effect could be quantified as a term of ???. ???.

壓縮코일스프링의 設計自動化

권혁홍,최선준 韓國工作機械學會 2000 한국생산제조학회지 Vol.9 No.6

Springs for vehicle suspension control the vibration of a car and influence on the ridability, safety, and life of a car. In the paper, the computer aided design program has been developed, which design the leaf spring shape from the given spec-ifications using basic theory and the expert's knowledge, and the design results arc checked by the analysis theory in order to increase the accuracy, and feed back to the design input. For the purpose of easy use, this program consists of pull-down menu and interactive input mode. To prove the effec-tiveness of this program, two springs, of which one is symmetric, other asymmetric, arc designed and analyzed, and the outputs are compared to the experiments. Considering the tolerance of the given specifications, the results are good.