http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
AtPDR12 contributes to lead resistance in Arabidopsis.
Lee, Miyoung,Lee, Kiyoul,Lee, Joohyun,Noh, Eun Woon,Lee, Youngsook American Society of Plant Physiologists 2005 Plant Physiology Vol.138 No.2
<P>Arabidopsis (Arabidopsis thaliana) contains about 130 ATP-binding cassette (ABC) proteins, which are likely to contribute to the transport of diverse materials, including toxic substances. However, the substrates of ABC transporters remain unknown in most cases. We tested which ABC transporter is involved in detoxification of lead [Pb(II)]. Among the many tested, we found that the message level of only AtPDR12 increased in both shoots and roots of Pb(II)-treated Arabidopsis, suggesting that it may be involved in the detoxification of Pb(II). AtPDR12-knockout plants (atpdr12) were used to further test this possibility. In Pb(II)-containing medium, atpdr12 plants grew less well and had higher Pb contents than those of wild-type plants. In contrast, AtPDR12-overexpressing Arabidopsis plants were more resistant to Pb(II) and had lower Pb contents than wild-type plants. The mutant phenotypes and their Pb contents, as well as the localization of the GFP:AtPDR12 fusion protein at the plasma membrane, suggest that AtPDR12 functions as a pump to exclude Pb(II) and/or Pb(II)-containing toxic compounds from the cytoplasm. Inhibition of glutathione synthesis by addition of buthionine sulfoximine to the growth medium exacerbated the Pb(II)-sensitive phenotype of atpdr12 plants, consistent with a glutathione-dependent detoxification mechanism operating in parallel with an AtPDR12-dependent mechanism. Thus, we propose that AtPDR12 is an ABC transporter that contributes to Pb(II) resistance in Arabidopsis.</P>
Quantitative shotgun proteomic analysis of rice anther under the cold stress
Joohyun Lee,Mijeong Kim,Yoonjung Lee 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
In rice, the stage of the meiosis in the pollen is sensitive stage resulted in the pollen sterility to reduce yield. Dianxi4 is a cold tolerant line. To monitoring the proteome expression patterns in the pollen of Dianxi4 under the cold stress, shotgun proteomic analysis was conducted to the anther of Dianxi4. The rice plant was grown in the peedy rice field then in the 10 DBH(days before heading), one individual rice plant was moved in the growth chamber under the condition of12℃/RH70%(12h day/12h night). Also the plant used as control was moved in the growth chamber unde the condition of 28℃/RH70%(12h day/12h night). after 4 days treatment, the plant were moved in a greenhouse. The treated rice anther were collected in the one day before heading. From the shotgun proteomic analysis, total of 3,855 non-redundant proteins were identified. Among them, 2,360 proteins were reproducibly identified through the treatment and replications. By the T-test, 1,181 differentially expressed proteins were detected. Through the GO analysis, proteins related in gene expression, cellular process, cellular biosynthetic process were enriched.
A quantitative shotgun proteomic study of rice grain development
Wondo Lee,Jaebok Cho,Hijin Kim,Joohyun Lee 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Although a great deal of rice proteomic research has been conducted, there are relatively few studies specifically addressing the rice grain proteome. The existing rice grain proteomic research has focused on the identification of deferentially expressed proteins. Here, we performed comparative shotgun proteomic analysis of rice grain development to construct an in-depth proteome reference map, to reveal the expression patterns of the identified proteins, and to detect proteins that are expressed deferentially during grain development. A Korean rice variety, Ilpumbyeo was used. Proteins were extracted from rice grains 10, 20, and 30 days after flowering, as well as from mature grains. The protein expression patterns were revealed by a quantitative shotgun proteoemic analysis. By merging all of the identified proteins in this study, we identified 4,172 non-redundant. A Genome Ontology category enrichment analysis for the 4,172 proteins revealed that 52 categories were enriched, including the carbohydrate metabolic process, transport, localization, lipid metabolic process, and secondary metabolic process. The relative abundances of the 1,784 reproducibly identified proteins were compared to detect 484 differentially expressed proteins during rice grain development. Clustering analysis and Genome Ontology category enrichment analysis revealed that proteins involved in the metabolic process were enriched through all stages of development, suggesting that proteome changes occurred even in the desiccation phase. Interestingly, enrichments of proteins involved in protein folding were detected in the desiccation phase and in fully mature grain.
Zigbee환경에서 효율적인 Cluster Header 선출 기법 (pp.346-350)
이주현(Joohyun Lee),이경화(Kyunghwa Lee),이준복(Junbok Lee),신용태(Yongtae Shin) 한국정보과학회 2010 정보과학회 컴퓨팅의 실제 논문지 Vol.16 No.3
현재, Zigbee환경에서 센서 노드는 자원 제약적인 특성 때문에 효율성을 높이기 위한 다양한 연구가 진행되고 있다.[1] 계층적 구조를 가지는 클러스터링 기법은 정보의 중복 전달 방지와 네트워크 확장 용이성을 제공한다.[2] 그러나 클러스터 헤더 선출 시 오버헤드 발생하며 잘못된 클러스터 헤더 선출은 자원을 효율적으로 사용할 수 없다는 한계가 있다. 본 논문에서는 계층적 클러스터링 기법에서 노드의 위치나 에너지 정보를 싱크노드에서 알고 있는 중앙 처리식을 활용하여 거리와 노드의 밀도를 기반으로 하는 클러스터 헤더의 선출기법을 제안하고자 한다. Since sensor nodes have restriction of using resources in Zigbee network, number of study on improving efficiency is currently ongoing[1]. Clustering mechanism based on hierarchy structure provides a prevention of duplicated information and a facility of a network expansion[2]. however overheads can occurs when the cluster header is elected and the election of a incorrect cluster header causes to use resourcesinefficiently. In this paper, we propose that the cluster header election mechanism using distances between nodes and density of nodes in accordance with the operation of the central processing system in which the sync nodes are having information of location and energy with respect to general nodes based on hierachy clustering mechanism.
Hijin Kim,Jaebok Cho,Wondo Lee,Joohyun Lee 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07
As the drought is getting worse, Lot of studies related to drought stress in plant have been conducted. Recently whole genome sequencing of Brassica rapa ssp which is important vegetable crop to East Asians has been completed to enable Omic research. It is known that the drought damages occur in the early stage of plant development. Here, we performed shotgun proteomics analysis of B. rapa to observe the morphological characters, monitor the expression patterns of the identified proteins during drought stress, and detect the proteins related to drought stress. The three week old B. rapa grown in density of single plant in a single pot were used. Drought stress were treated as that a single plant in soil was removed from the pot and the plant with soil was exposed to air and light without watering. Leaves were immediately harvested before drought treatment, 24hr after drought treatment, and 48hr after drought treatment. The protein expression patterns were monitored by a quantitative shotgun proteomics analysis. Extracted proteins were separated in 1D-SDS-PAGE then the gel sliced into seven pieces. Chopped gels were ingel-digested. Peptides were assigned to mass spectrometry (Q-Exactive). The ms/ms spectra were analyzed through Proteome Discoverer. By combining all of the identified proteins in the seven sliced gel samples, total B. rapa proteome reference map was completed. Protein expression patterns were investigated by comparing the quantity of protein. With shotgun proteomic approach, we evaluated the changes in the quantity and finally discovered the candidate proteins related with drought stress.