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Soo-JinCho,Jong-HoPark,SeongJooPark,Jong-SoonLim,EungHoKim,Yeon-JaeCho,Kwang-SooShin 한국미생물학회 2003 The journal of microbiology Vol.41 No.3
Extracellular protease, from Aeromonas hydrophila Ni 39, was purified 16.7-fold to electrophoretic homogeneity with an overall yield of 19.9%, through a purification procedure of acetone precipitation, and Q Sepharose and Sephacryl S-200 chromatographies. The isoelectric point of the enzyme was 6.0 and the molecular mass, as determined by Sephacryl S-200 HR chromatography, was found to be about 102 kDa. SDS/PAGE revealed that the enzyme consisted of two subunits, with molecular masses of 65.9 kDa. Under standard assay conditions, the apparent Km value of the enzyme toward casein was 0.32 mg/ml. About 90% of the proteolytic activity remained after heating at 60oC for 30 min. The highest rate of azocasein hydrolysis for the enzyme was reached at 60oC, and the optimum pH of the enzyme was 9.0. The enzyme was inhibited by the serine protease inhibitor, phenylmethylsulfonyl fluoride (PMSF), by about 87.9%, but not by E64, EDTA, pepstatin or 1,10-phenanthroline. The enzyme activity was inhibited slightly by Ca2+, Mg2+ and Zn2+ ions.
KyungSuk Seo,Kwang-SooLyoo,Yong-hoPark,Jong-HoKoh,Bong-KyunPark 대한수의학회 2002 Journal of Veterinary Science Vol.3 No.4
To determine the immune responses in pigs to hogcholera virus after treatment with an ionized alkalimineral complex (IAMC), 40 healthy pigs (28-32 daysold) from a commercial swine farm were purchasedand housed into 4 groups (n=10 each). All pigs werevaccinated intramuscularly (1 ml) with an attenuatedlive hog cholera virus (HCV, LOM strain) at 28-32days old and challenged with a virulent hog choleravirus at 8 weeks after vaccination. Each group wastreated with PowerFeelTMsprayed diet as 0.05% (w/w)in a final concentration (T-1,n=10),a diet mixed withSuperFeedTMas 3% (w/w) in a final concentration (T-2,n=10),or a diluted PowerFeelTMsolution (1:500,v/v) asdrinking water (T-3, n=10), respectively. A group(n=10) served as a non-treated control.Proportions ofexpressing CD2+ and CD8+ cells increased significantly(p<0.05) at 8-week post-application. Mean antibodytiters of each group against HCV gradually increasedto higher levels after vaccination and with challengeof the virulent virus.In conclusion,the IAMC-treateddiets can be helpful for the improvement of growth inpigs with proper vaccination program, while theIAMC-treated diets have no effects on the clinicalprotection against hog cholera.