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Soo-JinCho,Jong-HoPark,SeongJooPark,Jong-SoonLim,EungHoKim,Yeon-JaeCho,Kwang-SooShin 한국미생물학회 2003 The journal of microbiology Vol.41 No.3
Extracellular protease, from Aeromonas hydrophila Ni 39, was purified 16.7-fold to electrophoretic homogeneity with an overall yield of 19.9%, through a purification procedure of acetone precipitation, and Q Sepharose and Sephacryl S-200 chromatographies. The isoelectric point of the enzyme was 6.0 and the molecular mass, as determined by Sephacryl S-200 HR chromatography, was found to be about 102 kDa. SDS/PAGE revealed that the enzyme consisted of two subunits, with molecular masses of 65.9 kDa. Under standard assay conditions, the apparent Km value of the enzyme toward casein was 0.32 mg/ml. About 90% of the proteolytic activity remained after heating at 60oC for 30 min. The highest rate of azocasein hydrolysis for the enzyme was reached at 60oC, and the optimum pH of the enzyme was 9.0. The enzyme was inhibited by the serine protease inhibitor, phenylmethylsulfonyl fluoride (PMSF), by about 87.9%, but not by E64, EDTA, pepstatin or 1,10-phenanthroline. The enzyme activity was inhibited slightly by Ca2+, Mg2+ and Zn2+ ions.
세 자녀 이상 출산한 산모들을 대상으로 하여 지자체에서 시행한 무료 한약 지원 사업의 만족도 조사 -일개 한방병원을 중심으로 -
김수현 ( Soo-hyeonkim ),이은규 ( Eun-kyulee ),최유진 ( Yoo-jinchoe ),전영선 ( Yeong-seonjeon ),조은진 ( Eun-jinjo ),고홍제 ( Hong-jego ),유재희 ( Jae-huiyu ),박경미 ( Kyung-mipark ),조성희 ( Seong-huijo ),양승정 ( Seung-jeongyang 대한한방부인과학회 2018 大韓韓方婦人科學會誌 Vol.31 No.3
Objectives: This survey was conducted to investigate the satisfaction level of the project of providing free herbal medicines to mothers living in Suncheon who gave birth to more than three. Methods : Using the online survey tool Survey Monkey, the mother was sent a questionnaire in a cell phone text to answer the questions on their cell phones. The mothers’ contact information was based on the application form for a woman with three or more children, and a total of 27 people were surveyed and 20 responded. Results : The lowest level of satisfaction was 51 percent, the highest level of satisfaction was 100 percent, and the average was 86 percent. Conclusions : As the satisfaction level of the free herbal medicine support program is generally high, it is necessary to maintain the business continuously. And if other oriental medicine treatment is applied, more positive effect can be expected.