Establishment and Characterization of Mouse Avatar Models Derived from Patients with Hepatocellular Carcinoma

Jinsook Kim,Joong-Won Park,Sook-Kyung Lee,Bo Hyun Kim,Sung Hoon Kim,Eun Kyung Hong 한국간담췌외과학회 2014 한국간담췌외과학회 학술대회지 Vol.2014 No.4

The Genetic Characteristics of Multidrug-resistant Acinetobacter baumannii Coproducing 16S rRNA Methylase armA and Carbapenemase OXA-23

Jinsook Lim,Hye Hyun Cho,Semi Kim,Jimyung Kim,Kye Chul Kwon,Jong Woo Park,Sun Hoe Koo 대한미생물학회 2013 Journal of Bacteriology and Virology Vol.43 No.1

N100, N200, and P300 Auditory Event-Related Potentials Depending on Handedness and Response Tasks such as Button Pressing and Mental Counting

Jinsook Kim,Kieun Lee,Eunsung Lee 한국청각언어재활학회 2020 Audiology and Speech Research Vol.16 No.4

Purpose: This study was to determine the effects of response tasks, such as button pressing and mental counting, and handedness on N100, N200, and P300 auditory event-related potential (AERP). Methods: A total of 50 normal-hearing young adults with the average age of 21.6 (±1.5) years participated in this study. Among them, 15 men and 15 women were right-handed and 10 men and 10 women were left-handed. An oddball paradigm was used to deliver 30 stimuli of 2 kHz target tone bursts and 120 stimuli of 1 kHz nontarget tone bursts. The stimuli were presented at 70 dB sound pressure level with the rate of 1/s. Results: The button pressing task elicited significantly smaller N100 and larger P300 amplitudes than the mental counting task. N200 latency was significantly lower and P300 amplitude was higher in left-handed participants than those who are right-handed. Appearance percentages of right-/left-handed participants for N100, N200, and P300 were 80/95%, 85/85%, and 75/75% for the button pressing task and 80/90%, 80/80%, and 70/70% for mental counting task, respectively. Conclusion: The significant difference in appearance percentage between response tasks supported that P300 was a strong endogenous potential. N100 and N200 were thought to have both endogenous and exogenous characteristics. A more sensitive approach in selecting the task of response for the target stimuli and careful consideration for the handedness is necessary for AERP recordings.

Verbal Routines in a Blind Korean Child’s Language Acquisition

Jinsook Kim 한국언어학회 2013 언어 Vol.38 No.3

This study investigates the strategies that a blind child uses to learn language in the absence of visual input. For the last several decades, numerous studies on blind children’s language learning have reported that many blind children use formulaic expressions and verbal routines in their speech; these expressions are composed of several unanalyzed chunks and are used as whole utterances. In this study, the data were collected from one visually impaired Korean child between 24 and 30 months. The child was audio- and video-recorded, and all data were transcribed. The Korean blind child used many verbal routines and formulaic expressions. The results support the ‘use first, analyze later’ strategy (Peters, 1987) in that the child used verbal routines creatively in different conversational contexts and became aware of meaning and the usage of formulaic expressions. This study shows that blind children can be actively involved in their own language learning and that through verbal routines, they learn not only linguistic structures but also social interactions.

Pause or Continuation Signifiers for Convergence

Jinsook Kim 한국콘텐츠학회 2018 ICCC International Digital Design Invitation Exhib Vol.2018 No.5

Relationship between Job Satisfaction and Self-rated Health Status among Salaried Workers in Korea

Jinsook Kim(김진숙) 한국노동연구원 2007 노동정책연구 Vol.7 No.4

한국의 임금노동자에서 보이는 직업의 질과 직업만족도 간의 상관관계 그리고 직업만족도와 자가평가 건강수준 간의 상관관계가 유럽 또는 북미 노동자들의 경우와 어떻게 다른지 잘 알려져 있지 않다. 본 연구는 한국노동연구원이 2001년 시행한 제4차년도 「한국노동패널」조사 본조사와 건강과 은퇴 부가조사 표본을 이용하였다. 대다수의 조사 대상자들은 전일제(92%) 정규직(78%) 노동자들이었고, 68% 노동자들이 자신의 건강상태를 ‘매우 건강’하거나 ‘건강’하다고 평가하였다. 분석시 설정된 가정들이 대부분 받아들여졌는데, 인구학적 특성(연령, 성별, 혼인상태), 고용형태 관련 변수(정규직 여부), 기업형태 (사기업, 공기업(non-profit), 정부관련 기업)를 보정한 다중 회귀분석 모형에서 직무의 질은 직업만족도 및 자가평가 건강수준과 예상되었던 방향으로 유의한 상관관계를 보였다. 노동자들의 인구학적 특성, 객관적 건강상태, 직무 무관 생활만족도를 보정한 다중 로지스틱 회귀분석에서 직업 만족도는 자가평가 건강수준과 유의한 상관관계를 보였고, 직무의 질과 자가평가 건강수준 간의 상관관계를 매개하는 것으로 나타났다. This study sought to determine whether the relationship between job quality and job satisfaction and that between job satisfaction and health status among Korean salaried workers were similar to those found in studies of European and North American workers. This study uses a sample that includes salaried workers who completed both individual and additional (health and retirement) survey questionnaires from the 2001 Wave 4 Korea Labor and Income Panel Study (KLIPS). The majority of the respondents were working fulltime(92%) in a regular position(78%). Approximately two thirds of the respondents(68%) assessed their health as good or excellent. The hypothesized associations were robust after controlling for potential confounders. Regression analysis indicated a positive association between job quality and job satisfaction, controlling for demography (age, gender, marital status), employment type (regular employment or not), and employment sector (private, nonprofit, government-related, other). As expected, job satisfaction itself was significantly and positively associated with perceived health status, after taking account of workers’ demographic characteristics, objective health status, and non-work life satisfaction while mediating the effect of job quality on perceived health status.

The Role of Phonological Coding and Phonological Accessibility in Korean High School Students’ English Vocabulary Learning

Jinsook Kim 한국응용언어학회 2019 응용 언어학 Vol.35 No.4

The present study aims to investigate whether Korean EFL high school students engage in phonological coding when learning English vocabulary and whether phonological accessibility affects their vocabulary learning. Ninety-nine EFL Korean high school students participated in an experiment based on a 2x2 factorial design. The independent variables were learning condition (control versus articulatory suppression) and stimulus word type (phonologically regular versus irregular) and the dependent variable was short-term recall task scores. The findings showed a statistically significant main effect of learning condition and word type on the recall scores, and an interaction effect of the two independent variables on the recall scores. These results suggest that Korean EFL high school students do in fact engage in phonological coding when learning English vocabulary, that phonological accessibility is positively related to English vocabulary learning, and that interference with phonological coding is a more significant factor when learning phonologically irregular as opposed to regular words.

Crystal Structure of the Regulatory Domain of MexT, a Transcriptional Activator of the MexEF-OprN Efflux Pump in Pseudomonas aeruginosa

Kim, Suhyeon,Kim, Songhee H.,Ahn, Jinsook,Jo, Inseong,Lee, Zee-Won,Choi, Sang Ho,Ha, Nam-Chul Korean Society for Molecular and Cellular Biology 2019 Molecules and cells Vol.42 No.12

The Gram-negative opportunistic pathogen, Pseudomonas aeruginosa, has multiple multidrug efflux pumps. MexT, a LysR-type transcriptional regulator, functions as a transcriptional activator of the MexEF-OprN efflux system. MexT consists of an N-terminal DNA-binding domain and a C-terminal regulatory domain (RD). Little is known regarding MexT ligands and its mechanism of activation. We elucidated the crystal structure of the MexT RD at 2.0 Å resolution. The structure comprised two protomer chains in a dimeric arrangement. MexT possessed an arginine-rich region and a hydrophobic patch lined by a variable loop, both of which are putative ligand-binding sites. The three-dimensional structure of MexT provided clues to the interacting ligand structure. A DNase I footprinting assay of full-length MexT identified two MexT-binding sequence in the mexEF-oprN promoter. Our findings enhance the understanding of the regulation of MexT-dependent activation of efflux pumps.

Crystal structure of GSK3β in complex with the flavonoid, morin

Kim, Kuglae,Cha, Jeong Seok,Kim, Jin-Sik,Ahn, Jinsook,Ha, Nam-Chul,Cho, Hyun-Soo Elsevier 2018 Biochemical and biophysical research communication Vol.504 No.2

<P><B>Abstract</B></P> <P>GSK3β is a key kinase that plays a role in cellular signaling pathways. In Alzheimer's disease (AD), GSK3β has been implicated in hyperphosphorylation of tau proteins in the neuron, which is a hallmark of AD. Morin, a flavonoid that is abundant in nature, was found as an inhibitor of GSK3β that can reduce tau pathology <I>in vivo</I> and <I>in vitro</I>. In this study, we determined the crystal structure of GSK3β in complex with morin. The structure revealed that morin inhibits GSK3β by binding to the ATP binding pocket. Our findings augment the potential of morin as a functional food to help prevent AD, as well as to provide structural information to develop new therapeutics based on the morin skeleton.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Biochemical Analysis of Six Genetic Variants of Error-Prone Human DNA Polymerase ι Involved in Translesion DNA Synthesis

Kim, Jinsook,Song, Insil,Jo, Ara,Shin, Joo-Ho,Cho, Hana,Eoff, Robert L.,Guengerich, F. Peter,Choi, Jeong-Yun American Chemical Society 2014 Chemical research in toxicology Vol.27 No.10

<P/><P>DNA polymerase (pol) ι is the most error-prone among the Y-family polymerases that participate in translesion synthesis (TLS). Pol ι can bypass various DNA lesions, e.g., <I>N</I><SUP>2</SUP>-ethyl(Et)G, <I>O</I><SUP>6</SUP>-methyl(Me)G, 8-oxo-7,8-dihydroguanine (8-oxoG), and an abasic site, though frequently with low fidelity. We assessed the biochemical effects of six reported genetic variations of human pol ι on its TLS properties, using the recombinant pol ι (residues 1–445) proteins and DNA templates containing a G, <I>N</I><SUP>2</SUP>-EtG, <I>O</I><SUP>6</SUP>-MeG, 8-oxoG, or abasic site. The Δ1–25 variant, which is the <I>N</I>-terminal truncation of 25 residues resulting from an initiation codon variant (c.3G > A) and also is the formerly misassigned wild-type, exhibited considerably higher polymerase activity than wild-type with Mg<SUP>2+</SUP> (but not with Mn<SUP>2+</SUP>), coinciding with its steady-state kinetic data showing a ∼10-fold increase in <I>k</I><SUB>cat</SUB>/<I>K</I><SUB>m</SUB> for nucleotide incorporation opposite templates (only with Mg<SUP>2+</SUP>). The R96G variant, which lacks a R96 residue known to interact with the incoming nucleotide, lost much of its polymerase activity, consistent with the kinetic data displaying 5- to 72-fold decreases in <I>k</I><SUB>cat</SUB>/<I>K</I><SUB>m</SUB> for nucleotide incorporation opposite templates either with Mg<SUP>2+</SUP> or Mn<SUP>2+</SUP>, except for that opposite <I>N</I><SUP>2</SUP>-EtG with Mn<SUP>2+</SUP> (showing a 9-fold increase for dCTP incorporation). The Δ1–25 variant bound DNA 20- to 29-fold more tightly than wild-type (with Mg<SUP>2+</SUP>), but the R96G variant bound DNA 2-fold less tightly than wild-type. The DNA-binding affinity of wild-type, but not of the Δ1–25 variant, was ∼7-fold stronger with 0.15 mM Mn<SUP>2+</SUP> than with Mg<SUP>2+</SUP>. The results indicate that the R96G variation severely impairs most of the Mg<SUP>2+</SUP>- and Mn<SUP>2+</SUP>-dependent TLS abilities of pol ι, whereas the Δ1–25 variation selectively and substantially enhances the Mg<SUP>2+</SUP>-dependent TLS capability of pol ι, emphasizing the potential translational importance of these pol ι genetic variations, e.g., individual differences in TLS, mutation, and cancer susceptibility to genotoxic carcinogens.</P>