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Rong Wan,Jingran Liu,Zhengmei Yang,Panpan Zhu,Qinghe Cao,Tao Xu 한국유전학회 2020 Genes & Genomics Vol.42 No.3
Background DEAD-box protein family is the largest subfamily of RNA helicases and plays an important role in RNA metabolism and plant growth, development, and stress responses. Although DEAD-box genes have been characterized in various major crop plants, their identification and characterization in Convolvulaceae is still in infancy. Sweet potato (Ipomoea batatas, in Convolvulaceae) is the seventh most important crop in the world. Ipomoea trifida is one of the ancestors of sweet potato and is an effective resource for sweet potato cross-breeding. Objective Identification and characterisation of DEAD-box transcription factors in sweet potato wild ancestor I. trifida genome. Method A systematic genome-wide analysis was conducted to identify the DEAD-box transcription factors in the I. trifida genome. Results We identified 17 ItfDEAD-box genes which distributed unevenly on the nine chromosomes of I. trifida and encoded 29 DEAD transcripts. The phylogenetic analysis classified the DEAD-box proteins into nine groups named I–IX. Homology model prediction of ItfDEAD-box proteins obtained 14 models which lay a preliminary foundation for the further functional exploration of the ItfDEAD-box proteins. The tissue-specific and abiotic stress-responsive expression profiles of ItfDEAD-box genes were analyzed in different tissues and under abiotic stress responses by RNA-seq data and confirmed by quantitative PCR analysis. Some genes were significantly up- or down-regulated by different abiotic stress, suggesting that ItfDEAD-box plays a crucial role in stress responses in I. trifida. Conclusion The identification and gene expression of the ItfDEAD-box gene family might shed light on the function exploration of DEAD-box gene in I. trifida and promote the molecular breeding of sweet potato.
Thermal management analysis of serial-connection three-chamber piezoelectric pump
Lipeng He,Xiaoqiang Wu,Zheng Zhang,Jingran Wang,Dianbin Hu,Yamei Liu,Guangming Cheng 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.10
The design of a micropump with high performance and excellent reliability on thermal management still remains a challenge for electronic cooling. The performance of a piezoelectric pump with 3 chambers connected in series on thermal management was investigated for electronic cooling in this study. Two S-shaped channel heat sinks and the piezoelectric pump with 3 chambers connected in series were combined to form a loop cooling system. With water as the pumping medium, the tested pump has a flow rate most up to 690.6 ml/min at an input voltage of 220 V and an input frequency of 110 Hz. Suitability of the system has been demonstrated through simulation analysis and experimental verification for temperature control. A temperature reduction of 3 °C of CPU could be achieved at 250 mL/min flow rate generated by the piezoelectric pump with 3 chambers connected in series.