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Three-Dimensional Analysis of Chloroplast Structures Associated with Virus Infection
Jin, Xuejiao,Jiang, Zhihao,Zhang, Kun,Wang, Pengfei,Cao, Xiuling,Yue, Ning,Wang, Xueting,Zhang, Xuan,Li, Yunqin,Li, Dawei,Kang, Byung-Ho,Zhang, Yongliang American Society of Plant Biologists 2018 Plant Physiology Vol.176 No.1
<P>Three-dimensional visualization identifies structural remodeling in chloroplasts during barley stripe mosaic virus infection.</P><P>Chloroplasts are multifunctional organelles whose morphology is affected by environmental stresses. Although the three-dimensional (3D) architecture of thylakoid membranes has been reported previously, a 3D visualization of chloroplast under stress has not been explored. In this work, we used a positive-strand RNA ((+)RNA) virus, barley stripe mosaic virus (BSMV) to observe chloroplast structural changes during infection by electron tomography. The analyses revealed remodeling of the chloroplast membranes, characterized by the clustering of outer membrane-invaginated spherules in inner membrane-derived packets. Diverse morphologies of cytoplasmic invaginations (CIs) were evident with spherules at the periphery and different sized openings connecting the CIs to the cytoplasm. Immunoelectron microscopy of these viral components verified that the aberrant membrane structures were sites for BSMV replication. The BSMV αa replication protein localized at the surface of the chloroplasts and played a prominent role in eliciting chloroplast membrane rearrangements. In sum, our results have revealed the 3D structure of the chloroplasts induced by BSMV infection. These findings contribute to our understanding of chloroplast morphological changes under stress conditions and during assembly of plant (+)RNA virus replication complexes.</P>
Yueqing Zhao,Taiqiang Cao,Guangxu Pan,Jin Dai,Xiaoying Guo,Min Zheng,Xuan Lin 전력전자학회 2023 JOURNAL OF POWER ELECTRONICS Vol.23 No.9
Taking the permanent magnet synchronous motor (PMSM) as the research object, the model prediction pulse pattern control (MP3C) of a PMSM running in the medium–low-speed zone is adopted to further reduce the torque ripple and phase current total harmonic distortion (THD) of a motor running in the medium–low-speed zone. First, the objective function of the predictive pulse control method is optimized. The objective function is intended to be the difference between the switching voltage vector and the equivalent reference voltage vector. At the same time, the integral of the difference between the d-axis current reference value and the actual value is compensated into the voltage reference vector. Thus, the pulse pattern control selected by the objective function is optimal when the motor is running in the medium–low-speed region. In addition, the PMSM can run stably and reliably. Simulation results show that the torque ripple is reduced by 1.3 Nm and 1.2 Nm, and that the phase current total harmonic distortion is reduced by 0.10% and 0.03% when the motor is running at 5 Nm and 10 Nm and at a speed of 100 rpm, respectively. When the rotation speed is 1000 rpm, the torque ripple is reduced by 0.74 Nm and 0.78 Nm respectively. In addition, the phase current total harmonic distortion is decreased by 0.44% and 0.54%, respectively.
Chang-Yu Zhou,Ying Wang,Dao-Dong Pan,Jin-Xuan Cao,Yin-Ji Chen,Yuan Liu,Yang-Ying Sun,Chang-Rong Ou 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.3
Twelve pieces of longissimus dorsi were processed into Chinese traditional dry-cured loins. The changes in the proteolylic enzymes activities, myofibrillar proteins degradation, and free amino acids content were investigated during processing. Compared with fresh piece (0 day), the cathepsin B ? L and calpains activities decreased after dry-curing and maintained potential activities values of 23.25 and 15.04% in the final products, respectively. The myosin heavy chain (MHC) and C protein were intensely degraded at the dry-ripened stage; the 50 kDa desmin increased at day 2 and then disappeared at day 11. The total free amino acids content increased from 333.18 mg/100 g in the raw to 1096.54 mg/100 g at the end of the dry-ripening. This work provided a mechanism for the accumulation of free amino acids and predicted the proteolysis extent of myofibrillar proteins by monitoring the changes of three marker proteins (MHC, C protein and 50 kDa desmin) during Chinese traditional dry-cured loins processing. Keywords