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RISS 인기검색어
- 검색키워드
- 저자 : Jilan A. Nazeam (검색결과 4 건)
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Jilan A. Nazeam,Hala M. El-Hefnawy,Abdel-Naser B. Singab 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.8
Plant polysaccharides gained extended scientific attention for their immunomodulatory effect. However, few scientific studies structurally defined polysaccharides in relation to their biological modifier response. Therefore, the study explored the effect of structurally identified isolated macromolecules from Aloe arborescens against cytokine modulation (interferon [IFN-γ], interleukins [IL-2 and IL-12], and tumor necrosis factor [TNF-α]) in vitro. The structures were elucidated by GC, GPC, FT-IR spectroscopy, 1D NMR, COSY, HMBC, and HSQC. Two acetylated glucomannans (AANP4 and AAAP6), one deoxy-glucogalactan (AANP5), and one deoxy-N-acetyl-[1–4]-galactosamine (AANP2) were isolated. The results showed significant induction for all cytokines and the most potent component was AAAP6; acetylated phenolic glucomannan with a (1 → 3)-linked glucose–mannose and (1 → 4)-linked mannose backbone, which stimulated IL-12 by more than 10-fold compared with phytohemagglutinin (positive control). In conclusion, A. arborescens polysaccharides could be a landmark for development of effective immunotherapeutics against cancer and chronic inflammatory conditions.
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Middle Eastern Plants with Potent Cytotoxic Effect Against Lung Cancer Cells
Jilan A. Nazeam,Soad Z. EL-Emam 한국식품영양과학회 2024 Journal of medicinal food Vol.27 No.2
Cancer is one of the leading causes of increasing global mortality with uprising health concerns and threats. Unfortunately, conventional chemotherapy has substantial side effects, limiting its relevance and prompting a quest for safe andefficient alternatives. For thousands of years, plants have provided a rich reservoir for curing a variety of ailments, includingcancer. According to the World Health Organization, medicinal plants would be the best source of medications. However, only25% of drugs in the present pharmacopoeia are derived from plants. Hence, further research into different plants is required tobetter understand their efficacy. Twenty extracts of widely distributed Middle Eastern plants were screened for the cytotoxiceffect against lung cancer cell lines (A549). Eleven plants showed IC50 below 25 lg/mL, consequently, the bioactive extractswere further fractionated by graded precipitation using absolute ethanol. All fraction A (FA; crude polysaccharides precipitate)showed potent IC50, 0.2–5.5 lg/mL except the FA of Brassica juncea, Silybum marianum, and Phaseolus vulgaris, whereas FBfractions (filtrate) of Anastatica hierochuntica, Plantago ovate, Tussilago farfara, and Cucurbita moschata had lower efficacythan other fractions with IC50 values in the range of 0.1–7.7 lg/mL. The fractions of FA Taraxacum officinale and FB Ziziphusspina possess the most potent cytotoxic activity with IC50, 0.2 and 0.1 lg/mL, respectively. Moreover, cell cycle analysis of bothfractions revealed an arrest at G1/S-phase and activation of apoptosis rather than necrosis as the mode of cell death. Therefore, T. officinale and Z. spina fractions may pave the way to manage lung carcinoma as an alternative and complementary food regimen.
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Jilan A. Nazeam,Hala M. El-Hefnawy,Abdel-Naser B. Singab 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.10
Three new galactomannan components designed as AANP-1, AANP-3, and AAAP-7 were isolated from previously purified Aloe arborescence polysaccharide fractions, using ion-exchange, gel filtration, and preparative chromatographic techniques. Based on Fourier transform-infrared, one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy, the main backbone of AANP-1 elucidated as (1 → 4)-linked_α-d-deoxyGalp, (1 → 4) d-Manp, and β-d-2-glup. The sugar residue sequence of AANP-3 was (1 → 4)-linked β-d-NHAC-GAlp with β-d-AcManp side chain that attached to O-4 position. The AAAP-7 repeated units were (1 → 4) d-Manp linked with (1 → 4) d-Galp interspersed with (1 → 3)-α-Manp. The results of high performance size-exclusion chromatography indicated that the approximate molecular weights of AANP-1, AANP-3, and AAAP-7 were 4.2, 2.4, and 2.5 kDa, respectively. The cytokine modulation assay revealed that the isolated components promoted a remarkable release of interleukin (IL)-12 and tumor necrosis factor alpha in comparison with the negative control group, whereas potent significant induction of IL-2 and interferon gamma was detected more than the positive control phytohemagglutinin (P < .05). This is the first report for isolation galactomannans from Aloe arborescence. Moreover, this finding could provide new insights for exploring the biological modifier mechanism in correlation with the knowledge of structural configuration.
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Aloe arborescens Polysaccharides: In Vitro Immunomodulation and Potential Cytotoxic Activity
Jilan A. Nazeam,Haidy A. Gad,Ahmed Esmat,Hala M. El-Hefnawy,Abdel-Naser B. Singab 한국식품영양과학회 2017 Journal of medicinal food Vol.20 No.5
Different polysaccharides were isolated from the leaves of Aloe arborescens using the gradient power of hydrogen followed by antitumor and immunomodulatory assay. The total polysaccharide content of different fractions, water-soluble polysaccharide (WAP), acid-soluble polysaccharide (ACP), and alkaline-soluble polysaccharide (ALP), was estimated using a phenol-sulfuric acid spectrophotometric method. WAP possessed a higher content of mannose and glucose than either ACP or ALP. In vitro antitumor activity was investigated in three different cancer cell lines, and in vitro immunomodulatory potential was assessed through phagocytosis and lymphocyte transformation assay. The results showed that WAP and ALP exhibited the most significant cytotoxicity against HepG2 human liver cancer cells, with IC50 values of 26.14 and 21.46 μg/mL, respectively. In contrast, ALP was able to enhance lymphocyte transformation, whereas WAP had the most potent phagocytic activity. Molecular weight, total sugar and uronic acid content, Fourier transform-infrared analysis, and linkage type of bioactive polysaccharides were investigated. These findings revealed that the potential antitumor activity of the natural agents WAP and ALP was through an immunomodulation mechanism, which verifies the use of the plant as adjuvant supplement for cancer patients suffering immunosuppression during chemotherapy.
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