Association of bovine carcass phenotypes with genes in an adaptive thermogenesis pathway

Ryu, Jihye,Kim, Younyoung,Kim, Changdong,Kim, Jongbok,Lee, Chaeyoung Springer-Verlag 2012 Molecular biology reports Vol.39 No.2

Profile of Differential Promoter Activity by Nucleotide Substitution at GWAS Signals For Multiple Sclerosis

Ryu, Jihye,Woo, Jeyoung,Shin, Jimin,Ryoo, Hyunju,Kim, Younyoung,Lee, Chaeyoung by Lippincott WilliamsWilkins, Inc. 2014 Medicine Vol.93 No.28

ABSTRACT: This experimental study was conducted with completely randomized design.Genome-wide association studies (GWAS) have revealed a large number of genetic associations of nucleotide sequence variants with susceptibility to multiple sclerosis (MS). Nevertheless, studies to identify the functional relevance of these variants lag far behind identification of the GWAS signals. Expression quantitative trait loci (eQTLs) analysis and promoter activity analysis with the variants filtered by GWAS were conducted to identify their functional alleles and haplotypes. The promoter activity was assayed with reporter constructs containing variants at 8 MS GWAS signals resulted from 18 GWAS.The promoter activity differed by alternative sequence variants at upstream regions of the CYP24A1, CYP27B1, SYK, RAD21L1, PVR, ODF3B, and RGS14 genes (P < 0.05). The transcriptional regulations of sequence variants were also found by identifications of eQTLs for their corresponding genes with lymphoblastoid cells in the current study (SYK, ODF3B, RGS14, and PVR, P < 8.33 × 10) and with dendritic cells in a previous study (CYP27B1, P = 1.84 × 10).This study identified regulatory nucleotide sequences in the promoters of the CYP24A1, CYP27B1, SYK, RAD21L1, PVR, ODF3B, and RGS14 genes, and their variants differentially affected gene expression. This might result in their associations with MS susceptibility in previous GWAS. Further functional studies are required to understand the process of transcriptional regulation of the variants identified in the current study and the mechanisms underlying susceptibility to MS.

Effect of Aurea helianthus Extracts on Bone Formation in vitro

Jihye Ryu,Bokyung Kim,Mi hwa Park,Kyung Im Jung,Mihyang Kim 한국식품영양과학회 2020 한국식품영양과학회 학술대회발표집 Vol.2020 No.10

Hydrothermal preparation of carbon microspheres from mono-saccharides and phenolic compounds

Ryu, Jihye,Suh, Young-Woong,Suh, Dong Jin,Ahn, Dong June Elsevier 2010 Carbon Vol.48 No.7

<P><B>Abstract</B></P><P>Isolated carbon microspheres in a diameter range of 1–4μm were prepared by hydrothermal treatment of mono-saccharides (xylose for pentose and fructose for hexose) with phenolic compounds–phenol, resorcinol, and phloroglucinol. It was found that addition of these compounds, particularly phloroglucinol, into a sugar solution led to a substantial increase (roughly 20-fold) of the carbon yield. Mono-saccharides are dehydrated into furan compounds during hydrothermal carbonization, and these compounds subsequently react with phloroglucinol, as revealed by examination of high-performance liquid chromatograms. Through elemental analysis and spectroscopic studies, it was found that several oxygen functionalities including hydroxyl, carbonyl, carboxyl, ether, quinone, and ester groups were located throughout the carbon spheres derived from sugars and phloroglucinol. On the basis of the carbon formation mechanism, the C–O–C bond formed by linkage of OH groups of phenolic compounds preferentially exists at the core of carbon spheres whereas the outer carbon surface contains reactive oxygen functionalities such as hydroxyl, carbonyl, carboxylic, and ester groups, corresponding with results obtained with sugar-derived carbon materials.</P>

Cross Talk between the TM4SF5/Focal Adhesion Kinase and the Interleukin-6/STAT3 Pathways Promotes Immune Escape of Human Liver Cancer Cells

Ryu, Jihye,Kang, Minkyung,Lee, Mi-Sook,Kim, Hye-Jin,Nam, Seo Hee,Song, Haeng Eun,Lee, Doohyung,Lee, Jung Weon American Society for Microbiology 2014 Molecular and cellular biology Vol.34 No.16

<P>TM4SF5 overexpressed in hepatocellular carcinoma activates focal adhesion kinase (FAK) during tumor cell migration. However, it remains unknown how TM4SF5 in hepatocellular carcinoma cells compromises with immune actions initiated by extracellular cytokines. Normal and cancerous hepatocytes with or without TM4SF5 expression were analyzed for the effects of cytokine signaling activity on TM4SF5/FAK signaling and metastatic potential. We found that interleukin-6 (IL-6) was differentially expressed in hepatocytes depending on cancerous malignancy and TM4SF5 expression. IL-6 treatment activated FAK and STAT3 and enhanced focal adhesion (FA) formation in TM4SF5-null cells, but it decreased TM4SF5-dependent FAK activity and FA formation in SNU761-TM4SF5 cells. STAT3 suppression abolished the IL-6-mediated effects in normal Chang cells, but it did not recover the TM4SF5-dependent FAK activity that was inhibited by IL-6 treatment in cancerous SNU761-TM4SF5 cells. In addition, modulation of FAK activity did not change the IL-6-mediated STAT3 activity in either the Chang or SNU761 cell system. TM4SF5 expression in SNU761 cells caused invasive extracellular matrix degradation negatively depending on IL-6/IL-6 receptor (IL-6R) signaling. Thus, it is likely that hepatic cancer cells adopt TM4SF5-dependent FAK activation and metastatic potential by lowering IL-6 expression and avoiding its immunological action through the IL-6-STAT3 pathway.</P>

Effect of Abelmoschus manihot jinhuakui extract on collagen synthesis and osteogenesis in ovariectomized rats

Ryu Jihye,Bokyung Kim,Mi-Hwa Park,Seungcheol Lim,Mihyang Kim 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Abelmoschus manihot jinhuakui petal hot water extract(AJPH) was administered to ovariectomized female rats and the experimental groups were as follows: sham-operated rats (SHAM), ovariectomized rats (OVX-CON), ovariectomized rats supplemented with estradiol 10 μg/kg (OVX-E2) and ovariectomized rats supplemented with AJPH 50 or 200 mg/kg (OVX-AJPH50, OVX-AJPH200). Serum ALP activity was significantly increased in the OVX-CON group than in the SHAM group, on the other hand the OVX-AJPH200 group significantly decreased than the OVX-CON group, and showed a similar level to the SHAM group. The content of NTx and CTx in the serum was significantly increased in the OVX-CON group than in the SHAM group. Both OVX-E2 and AJPH groups decreased significantly compared to the OVX-CON group and decreased to the same level as the SHAM group. The collagen content in the bone and cartilage tissues was significantly decreased in the OVX-CON group with ovariectomy compared with the SHAM group, and increased in the OVX-E2 group and the AJPH groups. Therefore, the AJPH is expected to have a positive effect on bone by suppressing bone loss due to ovariectomy.

TM4SF5-Mediated Roles in the Development of Fibrotic Phenotypes

Ryu, Jihye,Lee, Jung Weon Carfax 2017 Mediators of inflammation Vol.2017 No.2

Idiopathic bone cavity: clinical and radiological features of 90 retrospective cases and surgical treatment

Jihye Ryu,Inhye Nam,Sang-Hun Shin,Yong-Deok Kim,Jae-Yeol Lee 대한구강악안면외과학회 2021 대한구강악안면외과학회지 Vol.47 No.5

Objectives: The purpose of this study was to evaluate the clinical and radiographic characteristics of idiopathic bone cavity (IBC) to determine the effect of surgical intervention on the process of healing. Materials and Methods: All cases diagnosed with IBC during the period of 2011 to 2020 at our Department of Oral and Maxillofacial Surgery were searched. Ninety cases were retrieved. The features evaluated were sex, age, contour of the lesion, number of teeth involved, site, history of trauma, and postoperative healing pattern. The significance of differences was assessed by Mann-Whitney U test and chi-square test. Results: The female:male ratio showed no predilection toward either sex (0.9:0.8). The mean age of the collected sample was 22.05±14.38 years, and the age ranged from 10 to 58 years. All cases presented in the mandible and showed well-circumscribed radiolucency. Margins were either scalloped or round in shape, and the size varied from one tooth to six teeth involvement. Seventy cases involved three or fewer roots. Three cases showed bilateral lesion. Four cases had a history of trauma at the area of the lesion. Fifty-one cases were followed for six months after surgery, and all showed increased bone density at the lesion. Conclusion: There is no definitive radiological or clinical feature of IBC. Considering the diversity of clinical and radiological features, such a diagnosis relies primarily on surgical findings of an empty bone cavity with no epithelial lining. Our data suggest that surgical intervention be the first choice of treatment as opposed to observation.

Genomic Heritability of Bovine Growth Using a Mixed Model

Ryu, Jihye,Lee, Chaeyoung Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.11

This study investigated heritability for bovine growth estimated with genomewide single nucleotide polymorphism (SNP) information obtained from a DNA microarray chip. Three hundred sixty seven Korean cattle were genotyped with the Illumina BovineSNP50 BeadChip, and 39,112 SNPs of 364 animals filtered by quality assurance were analyzed to estimate heritability of body weights at 6, 9, 12, 15, 18, 21, and 24 months of age. Restricted maximum likelihood estimate of heritability was obtained using covariance structure of genomic relationships among animals in a mixed model framework. Heritability estimates ranged from 0.58 to 0.76 for body weights at different ages. The heritability estimates using genomic information in this study were larger than those which had been estimated previously using pedigree information. The results revealed a trend that the heritability for body weight increased at a younger age (6 months). This suggests an early genetic evaluation for bovine growth using genomic information to increase genetic merits of animals.

Relationship between Nutrition Intake and Estimated Glomerular Filtration Rate in Korean Adults (30-65 years) from the Korea National Health and Nutrition Examination Survey 2013-2014

( Jihye Ryu ),( So Ra Yoon ),( Won Suk An ),( Oh Yoen Kim ) 한국임상영양학회 2018 Clinical Nutrition Research Vol.7 No.2

This study aimed to examine the relationship between nutrition intake and estimated glomerular filtration rate (eGFR) indicating kidney function in Korean individuals without diabetes or cardiovascular disease. Study participants from the Korea National Health and Nutrition Examination Survey 2013-2014 (n = 4,378, 30-65 years) were classified by their eGFR levels (mL/min/1.732 ㎡): ≥ 120 (n = 299), 119-105 (n = 789), 104-90 (n = 1,578), 89-60 (n = 1,685), < 60 (n = 27). After adjusted for confounding factors (age, sex, cigarette smoking, alcohol drinking, total caloric intake [TCI], income status, education level, body mass index, and physical activity), blood pressure, low-density lipoprotein (LDL) cholesterol, aspartate aminotransferase, alanine aminotransferase, blood urea nitrogen, creatinine, fasting glucose, and glycated hemoglobin were higher, and high density lipoprotein cholesterol levels were lower in participants with lower eGFR levels than those with higher eGFR levels. n-3 fatty acid (FA) and n-6 FA (% of TCI/day) intake were also significantly higher in participants with higher eGFR levels than in those with lower eGFR levels. Based on the above results, participants were subdivided into 3 groups according to n-6 FA intake levels (Q1: ≥ 2.93%, n = 1,462; Q2: 2.92%-1.88%, n = 1,463; Q3: < 1.88%, n = 1,453). People consuming higher n-6 FAs, particularly the Q1 group showed higher eGFR levels and lower levels of LDL cholesterol and creatinine. In conclusion, higher intake of n-6 FAs within the range of dietary reference may be beneficial to maintain healthy kidney function.