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RISS 인기검색어
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- 저자 : Jie Xin Tong (검색결과 6 건)
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Development of a Molecular Marker for Fruiting Body Pattern in Auricularia auricula-judae
( Fang-jie Yao ),( Li-xin Lu ),( Peng Wang ),( Ming Fang ),( You-min Zhang ),( Ying Chen ),( Wei-tong Zhang ),( Xiang-hui Kong ),( Jia Lu ),( Yoichi Honda ) 한국균학회 2018 Mycobiology Vol.46 No.1
The fruiting body pattern is an important agronomic trait of the edible fungus Auricularia auricula-judae, and an important breeding target. There are two types of fruiting body pattern: the cluster type and the chrysanthemum type. We identified the fruiting body pattern of 26 test strains, and then constructed two different near-isogenic pools. Then, we developed sequence characterized amplified region (SCAR) molecular markers associated with the fruiting body pattern based on sequence-related amplified polymorphism (SRAP) markers. Ten different bands (189-522 bp) were amplified using 153 pairs of SRAP primers. The SCAR marker “SCL-18” consisted of a single 522-bp band amplified from the cluster-type strains, but not the chrysanthemum strains. This SCAR marker was closely associated with the cluster- type fruiting body trait of A. auricula-judae. These results lay the foundation for further research to locate and clone genes controlling the fruiting body pattern of A. auricula-judae.
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Li-Xin Lu,Fang-Jie Yao,Peng Wang,Ming Fang,You-Min Zhang,Wei-Tong Zhang,Xiang-Hui Kong,Jia Lu 한국미생물학회 2017 The journal of microbiology Vol.55 No.10
Auricularia auricula-judae is a traditional edible fungus that is cultivated widely in China. In this study, a genetic linkage map for A. auricula-judae was constructed using a mapping population consisting of 138 monokaryons derived from a hybrid strain (A119-5). The monokaryotic parent strains A14-5 and A18-119 were derived from two cultivated varieties, A14 (Qihei No. 1) and A18 (Qihei No. 2), respectively. In total, 130 simple sequence repeat markers were mapped. These markers were developed using the whole genome sequence of A. auricula-judae and amplified in A14-5, A18- 119, and the mapping population. The map consisted of 11 linkage groups (LGs) spanning 854 cM, with an average interval length of 6.57 cM. A testcross population was derived from crossing between the monokaryon A184-57 (from the wild strain A184 as a tester strain) and the mapping population. Important agronomic trait-related QTLs, including mycelium growth rate on potato dextrose agar for the mapping population, mycelium growth rate on potato dextrose agar and sawdust for the testcross population, growth period (days from inoculation to fruiting body harvesting), and yield for the testcross population, were identified using the composite interval mapping method. Six mycelium growth raterelated QTLs were identified on LG1 and LG4, two growth period-related QTLs were identified on LG2, and three yieldrelated QTLs were identified on LG2 and LG6. The results showed no linkage relationship between mycelium growth rate and growth period. The present study provides a foundation for locating genes for important agronomic characteristics in A. auricula-judae in the future.
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The Influence of Sex in Stroke Thrombolysis: A Systematic Review and Meta-Analysis
Mingsu Liu,Guangqin Li,Jie Tang,Yan Liao,Lin Li,YANGZHENG,Tongli Guo,Xin Kang,Maoting Yuan 대한신경과학회 2018 Journal of Clinical Neurology Vol.14 No.2
Background and Purpose There is increasing recognition of the importance of stroke infemales to both clinical and public health. The natural course of stroke is worse in femalesthan in males, but the evidence regarding sex disparities in the responses to thrombolysis instroke patents is still controversial. We compared outcomes after thrombolysis treatment betweenfemales and males. Methods Clinical trials reported in the Embase, PubMed, and Cochrane Library electronicdatabases up to March 13, 2017 were included in this analysis. Two reviewers independentlyextracted the data and conducted quality assessments. Statistical tests were performed to checkfor heterogeneity and publication bias. Sensitivity analysis was also performed to evaluate thestability of the conclusions. Results Sixteen reports involving 60,159 patients were available for analysis. The female patientswere a 0.89-fold [95% confidence interval (CI)=0.87–0.90, p<0.001], 0.89-fold (95%CI=0.87–0.91, p<0.001), and 1.24-fold (95% CI=1.11–1.36, p<0.001) more likely to obtain good,excellent, and poor functional outcomes, respectively, with no significant difference in thecomplications of symptomatic intracranial hemorrhage among the sexes [risk ratios (RR)=0.99,95% CI=0.92–1.07, p=0.81] after thrombolysis treatment. In addition, the prevalence of a goodfunctional outcome did not differ significantly between females and males in the intra-arterialthrombolysis (IAT) group (RR=1.05, 95% CI=0.85–1.29, p=0.67) in a subgroup analysis. Conclusions This study has demonstrated that females often exhibit a worse outcome thanmales after intravenous thrombolysis (IVT), whereas no relevant sex differences were found inoutcome or recanalization after IAT, with safety regarding hemorrhage complications fromthrombolysis being the same for the sexes. However, IVT should not be withheld from femalestroke patients solely based on their sex before the findings are confirmed in further large-scaleresearch.
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Qing-Qing Dong,Qiu-Tong Wang,Lei Wang,Ya-Xin Jiang,Mei-Ling Liu,Hai-Jie Hu,Yong Liu,Hao Zhou,Hong-Peng He,Tong-Cun Zhang,Xuegang Luo 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.4
Sulforaphane (SFN), a natural compound derived from cruciferous vegetables, has been proved to possess potent anti-cancer activity. SMYD3 is a histone methyltransferase which is closely related to the proliferation and migration of cancer cells. This study showed that SFN could dose-dependently induce cell cycle arrest, stimulate apoptosis, and inhibit proliferation and migration of gastric carcinoma cells. Accompanied with these anticancer effects, SMYD3 and its downstream genes, myosin regulatory light chain 9, and cysteine-rich angiogenic inducer 61, was downregulated by SFN. Furthermore, overexpression of SMYD3 via transfection could abolish the effects of SFN, suggesting that SMYD3 might be an important mediator of SFN. To the best of our knowledge, this is the first report describing the role of SMYD3 in the anti-cancer of SFN. These findings might throw light on the development of novel anti-cancer drugs and functional food using SFN-rich cruciferous vegetables.
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Clinical Features and Survival Analysis of Very Young (Age<35) Breast Cancer Patients
Wei, Xue-Qing,Li, Xing,Xin, Xiao-Jie,Tong, Zhong-Sheng,Zhang, Sheng Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10
Objectives: To compare the clinicalpathological features and prognosis between premenopausal breast cancer patients aged of <35 and ${\geq}35$ years old. Methods: The clinical data and survival status of 1498 cases premenopausal operable breast cancer treated in our hospital from 2002.1 to 2004. 12 were collected, 118 cases were aged <35. They were divided into 4 groups: Luminal A, Luminal B, HER2-positive, Triple-negative. The disease free survival (DFS) and overall survival (OS) were identified. Results: The 5-year DFS and OS rates were significantly lower in age<35 than in $age{\geq}35$ patients. In the Luminal B, HER2-positive, Triple-negative group, the 5-year recurrence risk was higher in age<35 than in $age{\geq}35$ patients, and age<35 patients' 5-year death risk was higher only in Luminal B, Triple-negative group. Regardless of whether lymph node involved, age<35 patients had a bad prognosis in both DFS and OS. Conclusions: Compared with premenopausal age ${\geq}35$ breast cancer, age<35 patients had a worse outcome.
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