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RISS 인기검색어
- 검색키워드
- 저자 : Jiang Yanfeng (검색결과 3 건)
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Biocatalytic Production of Glucosamine from N-Acetylglucosamine by Diacetylchitobiose Deacetylase
( Zhu Jiang ),( Xueqin Lv ),( Yanfeng Liu ),( Hyun-dong Shin ),( Jianghua Li ),( Guocheng Du ),( Long Liu ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.11
Glucosamine (GlcN) is widely used in the nutraceutical and pharmaceutical industries. Currently, GlcN is mainly produced by traditional multistep chemical synthesis and acid hydrolysis, which can cause severe environmental pollution, require a long prodution period but a lower yield. The aim of this work was to develop a whole-cell biocatalytic process for the environment-friendly synthesis of glucosamine (GlcN) from N-acetylglucosamine (GlcNAc). We constructed a recombinant Escherichia coli and Bacillus subtilis strains as efficient whole-cell biocatalysts via expression of diacetylchitobiose deacetylase (Dac<sub>ph</sub>) from Pyrococcus furiosus. Although both strains were biocatalytically active, the performance of B. subtilis was better. To enhance GlcN production, optimal reaction conditions were found: B. subtilis whole-cell biocatalyst 18.6 g/l, temperature 40°C, pH 7.5, GlcNAc concentration 50 g/l and reaction time 3 h. Under the above conditions, the maximal titer of GlcN was 35.3 g/l, the molar conversion ratio was 86.8% in 3-L bioreactor. This paper shows an efficient biotransformation process for the biotechnological production of GlcN in B. subtilis that is more environmentally friendly than the traditional multistep chemical synthesis approach. The biocatalytic process described here has the advantage of less environmental pollution and thus has great potential for largescale production of GlcN in an environment-friendly manner.
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A SEPIC Flyback DC-DC Converter with GaN Switching Device
Li Lanyu,Jiang Zhilin,Jiang Yanfeng 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.4
A high-efficiency interleaved DC–DC converter based on SEPIC topology is developed in the paper, which can be used for micro photovoltaic inverter. Two flyback transformers in parallel connection can be controlled with single gallium nitride (GaN) switching power device. This is especially useful for the cost-down solution for high power situation with multiple flyback transformers in parallel connection. Using the single GaN switching device for double flyback transformers can suppress the output voltage ripple, while reduce the cost. In the paper, the detailed theoretical derivation on the novel topology is conducted. The simulation on the converter is carried out to verify the theoretical analysis. Finally, the topology is implemented on PCB board, experimentally showing the feasibility of the proposed converter.
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Experimental and Modelling Study of the Denaturation of Milk Protein by Heat Treatment
Fang Qian,Jiayue Sun,Di Cao,Yanfeng Tuo,Shujuan Jiang,Guangqing Mu 한국축산식품학회 2017 한국축산식품학회지 Vol.37 No.1
Heat treatment of milk aims to inhibit the growth of microbes, extend the shelf-life of products and improve the quality of the products. Heat treatment also leads to denaturation of whey protein and the formation of whey protein-casein polymer, which has negative effects on milk product. Hence the milk heat treatment conditions should be controlled in milk processing. In this study, the denaturation degree of whey protein and the combination degree of whey protein and casein when undergoing heat treatment were also determined by using the Native-PAGE and SDS-PAGE analysis. The results showed that the denaturation degree of whey protein and the combination degree of whey protein with casein extended with the increase of the heat-treated temperature and time. The effects of the heat-treated temperature and heat-treated time on the denaturation degree of whey protein and on the combination degree of whey protein and casein were well described using the quadratic regression equation. The analysis strategy used in this study reveals an intuitive and effective measure of the denaturation degree of whey protein, and the changes of milk protein under different heat treatment conditions efficiently and accurately in the dairy industry. It can be of great significance for dairy product proteins following processing treatments applied for dairy product manufacturing.
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