Synthesis of Chiral Bisacylphosphine Oxides and Their Application as Initiators in Helix-Sense-Selective Photopolymerization

Jia-An Zhang,Nian-Fa Yang,Li-Wen Yang,Shi Wu,Ye-Hui Chen,Jin Zhang 한국고분자학회 2013 Macromolecular Research Vol.21 No.6

Three new chiral bisacylphosphine oxides, (-)-bis(menthylformyl)phenylphosphine oxide (1a), (+)-bis(2,4,6-trimethylbenzoyl)menthylphosphine oxide (2a), and (-)-bis(menthylformyl)menthylphosphine oxide (3a),were synthesized using menthol as a chiral source. The synthesized new chiral bisacylphosphine oxides were used as photoinitiators for the polymerization of 1-phenyldibenzosuberyl methacrylate (PDBSMA). The polymerization initiated by chiral bisacylphosphine oxides under UV light irradiation is helix-sense-selective. Among the three prepared chiral bisacylphosphine oxides, the helix-sense-selectivity of bis(menthylformyl)menthylphosphine oxide is highest. The highest specific rotation of poly(1-phenyldibenzosuberyl methacrylate) at 365 nm is +416. The feed molar ratio of monomer to chiral bisacylphosphine oxide and the polymerization temperature have an influence on the helix-sense-selectivity. The lower the feed molar ratio of monomer to chiral bisacylphosphine oxide, the better the helix-sense-selectivity is. The increase of polymerization temperature is propitious to increase the helix-senseselectivity of the photopolymerization.

Possible Epithelial Ovarian Cancer Association with HPV18 or HPV33 Infection

Zhang, Pei-Pei,Zhou, Lei,Cao, Jia-Shi,Li, Yi-Ping,Zeng, Zhi,Sun, Ni,Shen, Li,Zhu, Hao-Yue,Ruan, Yang,Zha, Wen-Ting,Wang, Xin-Yu,Zhang, Ke-Qiang,Zhang, Ran Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.6

The present study was conducted to investigate the prevalence of HPV infection in epithelial ovarian cancer (EOC) in Hunan province. DNA samples were collected from paraffin embedded ovarian tissue from 322 patients with EOC, 99 with ovarian benign tumors and 199 normal persons. The polymerase chain reaction and direct sequencing were used to identify the HPV types in the samples. The relationship between the infection of human papillomavirus (HPV) and the epithelial ovarian carcinoma (EOC) was investigated combined with clinical data. The prevalence of HPV18 and HPV33 in EOC group and benign group was higher than in the normal group. HPV18 and HPV33 may play a role in the development of both EOC and ovarian benign tumor and may participate in the development of EOC with traditional risk factors, family history and abortion, possibly exerting synergistic effects.

Fabrication of Photopolymer Hierarchical Micronanostructures by Coupling Electrospinning and Photolithography for SERS Substrates

Wen-Yi Zhang,Xin-Ze Xiao,Chao Lv,Jia Zhao,Gong Wang,Xuan Gu,Ran Zhang,Bin-Bin Xu,Dan-Dan Zhang,Ai-Wu Li,Yong-Lai Zhang,Hong-Bo Sun 한국고분자학회 2013 Macromolecular Research Vol.21 No.3

Reported here is the fabrication of photopolymer hierarchical micronanostructures through a combinative process of electrospinning and subsequent photolithography. Electrospun SU-8 (epoxy-based negative photoresist)nanofiber films have been patterned into gratings with periods of 100, 200, 300, and 400 μm, respectively. Deposition of a silver nanolayer on these interlaced nanofiber films would lead to the formation of various plasmonic nanostructures,and therefore, giving rise to abundant surface-enhanced Raman scattering (SERS) “hot spots”. In the detection of Rhodamine 6G (R6G), probing molecule, the resultant SERS substrates show both high sensitivity and good reproducibility. The SERS enhancement factor could reach as high as ~108, indicating high efficiency. The fabrication of patterned, highly efficient SERS substrates may hold a great promise for the integration of SERS substrates in various microdevices such as microfluidic chips.

Combination Doxorubicin and Interferon-α Therapy Stimulates Immunogenicity of Murine Pancreatic Cancer Panc02 Cells via Up-regulation of NKG2D ligands and MHC Class I

Wang, Wen-Jia,Qin, Si-Hao,Zhang, Ji-Wei,Jiang, Yue-Yao,Zhang, Jin-Nan,Zhao, Lei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.22

Background: Pancreatic adenocarcinoma is a malignant gastrointestinal cancer with significant morbidity and mortality. Despite severe side effects of chemotherapy, the use of immunotherapy combined with chemotherapy has emerged as a common clinical treatment. In this study, we investigated the efficacy of the combined doxorubicin and interferon-${\alpha}$ (IFN-${\alpha}$) therapy on murine pancreatic cancer Panc02 cells in vitro and in vivo and underlying mechanisms. Materials and Methods: A Panc02-bearing mouse model was established to determine whether doxorubicin and interferon-${\alpha}$ (IFN-${\alpha}$) could effectively inhibit tumor growth in vivo. Cytotoxicity of natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) was evaluated using a standard LDH release assay. To evaluate the relevance of NK cells and CD8 T cells to the combination therapy-mediated anti-tumor effects, they were depleted in tumor-bearing mice by injecting anti-asialo-GM-1 antibodies or anti-CD8 antibodies, respectively. Finally, the influence of doxorubicin+interferon-${\alpha}$ (IFN-${\alpha}$) on the ligands of NK and T cells was assessed by flow cytometry. Results: The combination therapy group demonstrated a significant inhibition of growth of Panc02 in vivo, resulting from activated cytotoxicity of NK cells and CTLs. Depleting CD8 T cells or NK cells reduced the anticancer effects mediated by immunochemotherapy. Furthermore, the doxorubicin+IFN-a treatment increased the expression of major histocompatibility complex class I (MHC I) and NKG2D ligands on Panc02 cells, suggesting that the combined therapy may be a potential strategy for enhancing immunogenicity of tumors. All these data indicate that the combination therapy using doxorubicin and interferon-${\alpha}$ (IFN-${\alpha}$) may be a potential strategy for treating pancreatic adenocarcinoma.

A Series of Transition-metal Coordination Complexes Assembled from 3-Nitrophthalic Acid and Thiabendazole: Synthesis, Structure and Properties

Wen-Jia Xu,Qi-Jun Xue,Peng Liang,Ling-Yu Zhang,Yan-Feng Huang,Yu Feng 대한화학회 2014 Bulletin of the Korean Chemical Society Vol.35 No.1

In order to explore new coordination frameworks with novel designed 3-nitrophthalic acid and the same N– donor ancillary ligand, a series of novel coordination complexes, namely, [Cd 2 (3-NPA) 2 (TBZ) 2 (H 2 O) 2 ]·2H 2 O (1), [Zn 2 (3-NPA) 2 (TBZ) 2 ] (2), [Zn 2 O(3-NPA)(TBZ)(H 2 O)] n (3), [Co(3-NPA)(TBZ)(H 2 O)] n (4) (3-NPAH 2 = 3- nitrophthalic acid), have been hydrothermally synthesized through the reaction of 3-nitrophthalic acid with divalent transition-metal salts in the presence of N-donor ancillary coligand (TBZ = thiabendazole). As a result of various coordination modes of the versatile 3-NPAH 2 and the coligand TBZ, these complexes exhibit structural diversity. X-ray structure analysis reveals that 1 and 2 are 0D molecular rings, while 3 and 4 are one- dimensional (1D) infinite chain polymers. And the weak O-H…O hydrogen bonds and C-H…O nonclassical hydrogen bonds as well as π-π stacking also play important roles in affecting the final structure where complexes 1, 3 and 4 have 3D supramolecular architectures, while complex 2 has a 2D supramolecular network. Also, IR spectra, fluorescence properties and thermal decomposition process of complexes 1-4 were investigated.

Association of CYP2E1 and NAT2 Polymorphisms with Lung Cancer Susceptibility among Mongolian and Han Populations in the Inner Mongolian Region

Zhang, Jing-Wen,Yu, Wan-Jia,Sheng, Xiao-Min,Chang, Fu-Hou,Bai, Tu-Ya,Lv, Xiao-Li,Wang, Guang,Liu, Su-Zhen Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Purpose: To explore associations of CYP2E1 and NAT2 polymorphisms with lung cancer susceptibility among Mongolian and Han populations in the Inner Mongolian region. Materials and Methods: CYP2E1 and NAT2 polymorphisms were detected by PCR-RFLP in 930 lung cancer patients and 1000 controls. Results: (1) Disequilibrium of the distribution of NAT2 polymorphism was found in lung cancer patients among Han and Mongolian populations (p=0.031). (2) Lung cancer risk was higher in individuals with c1, D allele of CYP2E1 RsaI/PstI, DraI polymorphisms and slow acetylation of NAT2 (c1 compared with c2, OR=1.382, 95%CI: 1.178-1.587, p=0.003; D compared with C, OR=1.241, 95%CI: 1.053-1.419, P<0.001; slow acetylation compared with rapid acetylation, OR=1.359, 95%CI:1.042-1.768, p=0.056) (3) Compared with c2/c2 and rapid acetylation, c1/c1 together with slow acetylation synergetically increased risk of lung cancer 2.83 fold. (4) Smokers with CYP2E1 c1/c1, DD, and NAT2 slow acetylation have 2.365, 1.916, 1.841 fold lung cancer risk than others with c2/c2, CC and NAT2 rapid acetylation, respectively. (5) Han smokers with NAT2 slow acetylation have 1.974 fold lung cancer risk than others with rapid acetylation. Conclusions: Disequilibrium distribution of NAT2 polymorphism was found in lung cancer patients among Han and Mongolian populations. Besides, Han smokers with NAT2 slow acetylation may have higher lung cancer risk compared with rapid acetylation couterparts. CYP2E1 c1/c1, DD and NAT2 slow acetylation, especially combined with smoking, contributes to the development of lung cancer. CYP2E1 c1/c1 or DD genotype and NAT2 slow acetylation have strong synergistic action in increasing lung cancer risk.

New hydroperoxylated and 20,24-epoxylated dammarane triterpenes from the rot roots of Panax notoginseng

Jia-Huan Shang,Wen-Jie Sun,Hong-Tao Zhu,Dong Wang,Chong-Ren Yang,Ying-Jun Zhang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.3

Background: Root rot is a serious destructive disease of Panax notoginseng, a famous cultivated araliaceousherb called Sanqi or Tianqi in Southwest China. Methods: The chemical substances of Sanqi rot roots were explored by chromatographic techniques. MS,1D/2D-NMR, and single crystal X-ray diffraction were applied to determine the structures. Murinemacrophage RAW264.7 and five human cancer cell lines were used separately for evaluating the antiinflammatoryand cytotoxic activities. Results and Conclusion: Thirty dammarane-type triterpenes and saponins were isolated from the rot rootsof P. notoginseng. Among them, seven triterpenes, namely, 20(S)-dammar-25-ene-24(S)-hydroperoxyl-3b,6a,12b,20-tetrol (1), 20(S)-dammar-3-oxo-23-ene-25-hydroperoxyl-6a,12b,20-triol (2), 20(S)-dammar-12-oxo-23-ene-25-hydroperoxyl-3b,6a,20-triol (3), 20(S)-dammar-3-oxo-23-ene-25-hydroperoxyl-12b,20-diol (4), 20(S),24(R)-epoxy-3,4-seco-dammar-25-hydroxy-12-one-3-oic acid (5), 20(S),24(R)-epoxy-3,4-seco-dammar-25-hydroxy-12-one-3-oic acid methyl ester (6), and 6a-hydroxy-22,23,24,25,26,27-hexanordammar-3,12,20-trione (7), are new compounds. In addition, 12 known ones (12e16 and 19e25)were reported in Sanqi for the first time. The new Compound 1 showed comparable antiinflammatoryactivity on inhibition of NO production to the positive control, whereas the known compounds 9, 12, 13,and 16 displayed moderate cytotoxicities against five human cancer cell lines. The results will providescientific basis for understanding the chemical constituents of Sanqi rot roots and new candidates forsearching antiinflammatory and antitumor agents.

mRNA Expression of Ovine Angiopoietin-like Protein 4 Gene in Adipose Tissues

Zhang, Jing,Jing, Jiong-Jie,Jia, Xia-Li,Qiao, Li-Ying,Liu, Jian-Hua,Liang, Chen,Liu, Wen-Zhong Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.5

Angiopoietin-like protein 4 (ANGPTL4) is involved in a variety of functions, including lipoprotein metabolism and angiogenesis. To reveal the role of ANGPTL4 in fat metabolism of sheep, ovine ANGPTL4 mRNA expression was analyzed in seven adipose tissues from two breeds with distinct tail types. Forty-eight animals with the gender ratio of 1:1 for both Guangling Large Tailed (GLT) and Small Tailed Han (STH) sheep were slaughtered at 2, 4, 6, 8, 10, and 12 months of age, respectively. Adipose tissues were collected from greater and lesser omental, subcutaneous, retroperitoneal, perirenal, mesenteric, and tail fats. Ontogenetic mRNA expression of ANGPTL4 in these adipose tissues from GTL and STH was studied by quantitative real time polymerase chain reaction. The results showed that ANGPTL4 mRNA expressed in all adipose tissues studied with the highest in subcutaneous and the lowest in mesenteric fat depots. Months of age, tissue and breed are the main factors that significantly influence the mRNA expression. These results provide new insights into ovine ANGPTL4 gene expression and clues for its function mechanism.

Increased Serum High Mobility Group Box 1 (HMGB1) Concentration and the Altered Expression of HMGB1 and Its Receptor Advanced Glycation Endproducts in Pemphigus

( Jia-yi Li ),( Yong-hong Lu ),( Li-wen Zhang ),( Pei-mei Zhou ),( Tao Chen ) 대한피부과학회 2017 Annals of Dermatology Vol.29 No.1

Morphological and Anatomical Differences among Three Seagrass Species in a High-energy Coastal Area Typically Dominated by Surfgrass in a Rocky Coastal Area of Shandong Peninsula, China

Wen Tao Li,Jia Song,Chongfeng Zhong,Xin Hou,Ran Cheng,Pei-dong Zhang 한국해양과학기술원 2020 Ocean science journal Vol.55 No.2

Surfgrass Phyllospadix iwatensis is the dominant seagrass species in the coastal area of Mashanli, which lies at the east end of Shandong Peninsula, China. To our surprise, some Zostera marina and Zostera caespitosa patches have been recently found adjoining the surfgrass meadows. How the two Zostera species adapt to the high-energy habitats where Phyllospadix spp. thrive, and what kind of differences there are in the morphology and anatomy among the three species are unknown. To understand their adaptation strategies to the environment, we observed their morphological feature and anatomical structure of the three seagrass species by optical microscope. The results showed that much narrower leaves were observed in P. iwatensis, much higher plant density was observed in Z. caespitosa and Z. marina and much lower plant height was observed in Z. marina, which might have contributed to their adaptation in the environment. While for the supporting tissues (the epidermis, mesophyll cells, and vascular bundles for leaves, as well as the epidermis, exodermis and stele for rhizomes), the number of mesophyll cells in the leaves and the proportion of the other tissues in the cross sections in the leaves and rhizomes were significantly different among species. The results suggested that the three species adapt to the high-energy environment through different strategies.