백내장수술시 점탄성물질인 Healon(R) 과 Viscoat(R) 사용에 따른 안압 및 각막내피세포의 변화

김지원(Ji Won Kim),한태원(Tae Won Hahn),김만수(Man Soo Kim),김재호(Jae Ho Kim) 대한안과학회 1992 대한안과학회지 Vol.33 No.3

다니엘 리베스킨트의 박물관 전시공간 구축방식에 관한 연구

한지원(Hahn Ji-Won) 대한건축학회 2007 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.27 No.1

In the Jewish Museum Berlin which is his first selected work in competition, Daniel Libeskind built a continuous exhibition space with a tortuous linear mass. He has designed eleven museums out of total thirty-two works; ten museums except the one in Fiera Milano project which has not been made any firm plans have shown a process of linear, nonlinear, and compositive exhibition spaces according to the continuance of exhibition experience, the shape of mass, and transiton space’s relations with exhibition space. The linear exhibition space has a linear movement in a linear mass; the nonlinear one has a nonlinear movement in a gathered linear mass or a crystal mass; and the com positive one has a linear movement in a crystal mass. At the same time, the linear exhibition space has a transition space which is independent or dependent from the exhibition space; the nonlinear one has a synthetic transition space; and the com positive one has a independent transition space. Accordingly, he has built the mass of musuems from a linear to a crystal or mixed one; but pursued that the exhibition space can make spectators have a continuous exhibition experience.

도축돈에서 분리된 살모넬라의 혈청형 및 유전형

최원종 ( Won Zong Choi ),정지헌 ( Ji Hun Jung ),원호근 ( Ho Keun Won ),강정무 ( Zheng Wu Kang ),한태욱 ( Tae Wook Hahn ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.1

Salmonella infections cause the disease in pigs but also some zoonotic Salmonella sero-types can be transmitted to human through swine products, resulting in food poisoning. The objective of this study was to investigate the bacteriological prevalence and detec-tion of invA gene using Salmonella specific polymerase chain reaction(PCR), the epidemio-logical characteristics related to Salmonella strains cultured from pig samples in Gang-won areas using serotyping, random amplified polymorphic DNA(RAPD) and pulsed-field gel electrophoresis(PFGE) methods. During the period of November 2001 through April 2002, 1,174 ileocecal lymph node were collected from the slaughtered pigs raised in 38 farms located in Gangwon province. The samples were submerged in boiling water and macerated in saline and lymph node homogenates were inoculated into Tetrathionate broth with iodine(TTB, Difco, 0.5% iodine was added) for enrichment growth. Then additional tests were performed using several mediums, and suspects were identified by API 20E kit (BioM?rieux) and PCR. Of total 1,174 samples from 38 farms, 44 (3.7%) were isolated as Salmonella spp from 13 farms (34.2%). Of 44 isolates, 31 were in Yangyang region, followed by 9 in Goseong, 2 in both Gangneung and Sokcho. However, there was no difference in regional isolation frequency. All isolates have a 521bp amplified product in Salmonella specific PCR with primer invA which encodes in proteins for invasion of epithelial cells. Of 44 recovered serotypes, 23(52.3%) were S Eingedi, 10(22.7%) S Schwarzengrund, 9(20.5%) S Typhimurium, and 2(4.5%) S Mbandaka. In RAPD analysis, there appeared to be unique bands distin-guishing each serotype, although similarities exist between the different serotypes. Four serotypes of 44 Salmonella isolates appeared to fall into 14 different RAPD types. In PFGE analysis, 9 S Typhimurium were tested with XbaI enzyme and SpeI enzyme. The combination of results obtained with two enzymes subdivided the 9 S Typhimurium into 4 PFGE types.

Non-premixed Hydrogen Flame Structure in Supersonic Coflowing Air Flows

Ji-Ho Kim,Jehung Kim,Youngbin Yoon,Chul Woung Park,Jae Won Hahn 한국연소학회 2002 한국연소학회지 Vol.7 No.1

Production and Characterization of Glucocerebrosidase in Transgenic Rice Cells

Ji-Yeon HAN,Seung-Hoon KANG,Hahn-Sun JUNG,Su-Hwan CHEON,Jun-Young KWON,Ji-Won CHOI,Dong-Il KIM 한국생물공학회 2009 한국생물공학회 학술대회 Vol.2009 No.10

Molecular Biological Identification of Malassezia Yeasts Using Pyrosequencing

( Ji Young Kim ),( Hyung Jin Hahn ),( Yong Beom Choe ),( Yang Won Lee ),( Kyu Joong Ahn ),( Kee Chan Moon ) 대한피부과학회 2013 Annals of Dermatology Vol.25 No.1

Background: A Pyrosequencing assay has been used in identification of fungal species such as Candida or Aspergillus and diagnosis of pathogenic bacteria such as Helicobacter pylori but there has been no report on successful isolation and identification of Malassezia yeasts using the pyrosequencing method. Objective: Examine the applicability and plausibility of the pyrosequencing method in identification of the Malassezia species. Methods: At internal transcribed spacer (ITS) sites 1 and 2, three primers were developed using Pyrosequencing Assay Design Software (Biotage AB). Pyrosequencing was performed on 11 standardstrains and 83 genomic DNA samples obtained from 66 healthy controls aged from 1 to 80. Results: The eleven Malassezia standard species and 83 genomic DNA samples were successfully identified using the pyrosequencing assay. Conclusion: The pyrosequencing method is a new tool for analysis of Malassezia yeasts, and its precision and rapidity suggests its clinical applicability. (Ann Dermatol 25(1) 73∼ 79, 2013)

A Web-based Health Information Survey for Korean Human Milk Bank and Cur-rent Issues

( Won-ho Hahn ),( Nam Mi Kang ),( Ji A Jung ),( Seunghyun Song ),( Suyeon Park ),( Hyun-jun Kim ) 대한주산의학회 2017 Perinatology Vol.28 No.2

Objective: We evaluated the Korean mothers` level of awareness of human milk bank (HMB) in order to find out the issues in establishing HMB in Korea. These mothers had infants younger than 18 months old to find out the issues that would help establishment of HMB in Korea. Methods: The survey questionnaire was developed by a team composed by nurses, neonatolo-gists, obstetricians, nutritionists, health care specialists and faculty members. The internet survey was performed by sending emails to mothers who are registered to M family members and have children younger than 18 months. In total, 864 questionnaire were gathered from 114,469 answers and the results were analyzed. Results: Fifty two point eight percent of the respondents had awareness of HMB. 79.4% of sub-jects agreed with the necessity of HMB. On the basis of multivariate logistic regression analysis, we found that the numbers of children, awareness of HMB, the concern on the HMB in Korea, and the expected problems in establishing HMB in Korea were significant factors when evaluating the necessity of HMB in Korea. The common factors associated with positive thinking of potential users, donors and recipients were the awareness of HMB, and the experience of feeding own breast milk to other`s babies. Conclusion: More than half of Korean women were found to agree with the necessity of HMB in Korea, even though they had concern on infection controls and management of HMB and the purpose and function of HMB was not widely educated. The establishment of HMB in Korea would be effective with public education and considering their demands.

Acupuncture Sample Injection for Microchip Capillary Electrophoresis and Electrokinetic Chromatography

Ha, Ji Won,Hahn, Jong Hoon American Chemical Society 2016 ANALYTICAL CHEMISTRY - Vol.88 No.9

<P>A simple nanoliter-scale injection technique was developed for polydimethylsiloxane (PDMS) microfluidic devices to form the well-defined sample plugs in microfluidic channels. Sample injection was achieved by performing acupuncture on a channel with a needle and applying external pressure to a syringe. This technique allowed us to achieve reproducible injection of a 3-nL segment into a microchannel for PDMS microchip-based capillary electrophoresis (CE). Capillary zone electrophoresis (CZE) and capillary electro-chromatography (CEC) with bead packing were successfully performed by applying a single potential in the most simplified straight channel. The advantages of this acupuncture injection over the electrokinetic injection in microchip CE include capability of minimizing sample loss and voltage control hardware, capability of serial injections of different sample solutions into a same microchannel, capability of injecting sample plugs into any position of a microchannel, independence on sample solutions during the loading step, and ease in making microchips due to the straight channel, etc.</P>

Identification of Antiangiogenic Potential and Cellular Mechanisms of Napyradiomycin A1 Isolated from the Marine-Derived <i>Streptomyces</i> sp. YP127

Hwang, Ji Sun,Kim, Geum Jin,Choi, Hyun Gyu,Kim, Min Cheol,Hahn, Dongyup,Nam, Joo-Won,Nam, Sang-Jip,Kwon, Hak Choel,Chin, Jungwook,Cho, Sung Jin,Hwang, Hayoung,Choi, Hyukjae American Chemical Society and American Society of 2017 Journal of natural products Vol.80 No.8

<P>Angiogenesis is the process of new blood vessel formation. Excessive angiogenesis is a critical factor in the progression of cancer, macular degeneration, and other chronic inflammatory diseases. When investigating the effects of crude extracts of cultured marine microorganisms, an extract of the cultured Streptomyces sp. YP127 strain was found to inhibit human umbilical vein endothelial cell (HUVEC) tube formation. Bioassay-guided fractionation and spectroscopic data analyses led to the identification of napyradiomycin A1 (1) as an antiangiogenic component of the extract. Compound 1 inhibited HUVEC tube formation in a concentration-dependent manner. It inhibited endothelial cell proliferation but did not affect human dermal fibroblast proliferation. Compound 1 also suppressed migration and invasion of vascular endothelial cells. In addition, compound 1 suppressed vascular endothelial cadherin expression and increased the permeability of the endothelial cell membrane. These results suggested that compound 1 modulates cell permeability and inhibits the angiogenesis of endothelial cells.</P>

횡경막상부에 국한된 임상적 병기 1-2기 호지킨병에서 치료 결과와 예후 인자의 분석

박원(Won Park),서창옥(Chang Ok Suh),정은지(Eun Ji Chung),조재호(Jae Ho Cho),정현철(Hyun Cheol Chung),김주항(Joo Hang Kim),노재경(Jae Kyung Roh),한지숙(Jee Sook Hahn),김귀언(Gwi Eon Kim) 대한방사선종양학회 1998 Radiation Oncology Journal Vol.16 No.2

목 적 : 시험개복술을 시행하지 않은 임상적 병기 1기, 2기 횡경막상부 호지킨병에서 후향적으로 치료 결과를 분석함으로써 시험개복술의 필요성을 재검토하였고 예후 인자를 분석하고, 예후 인자군의 유용성을 평가하고자 하였다. 대상 및 방법 : 1985년에서 1995년까지 연세의료원에서 횡경막 상부에 국한된 임상적 병기 1-2기 호지킨병으로 진단받고 치료를 받았던 51례를 대상으로 하였다. 연령은 4-67세까지 였으며 중앙값은 30세였고 병기별로는 ⅠA, ⅡA, ⅡB가 각각 16례, 25례, 10례였다. 방사선치료는 4, 6 MV 광자선으로 매일 1.5-1.8Gy씩 19.5Gy에서 55.6Gy(중앙값 : 45Gy)가 조사되었으며, 항암화학요법은 방사선치료 전후에 2-12회(중앙값 : 6회) 시행되었다. 방사선치료 단독, 항암화학방사선 병용요법, 항암화학요법 단독이 각 각 31례, 16례, 4례에 시행되었고 방사선치료는 involved field 방사선치료가 3례, 맨틀방사선치료가 26례, 부분적림프절방사선치료가 18례에서 시행되었다. 생존율 분석은 Kaplan-Meier method, 유의성 검증은 log-rank test를 이용하였다. 결 과 : 5년 전체 생존율은 87.6%, 무병 생존율은 78.0%였다. 완전 관해된 50례 중 림프절만 재발한 경우가 4례, 간이나 폐 또는 비장에서 재발한 경우가 3례, 림프절과 간에 동시에 재발된 경우가 1례 있었다. 재발한 8례중 구제 치료가 항암화학요법 단독, 방사선치료 단독, 항암화학방사선병용요법이 각 각 4례, 1례, 2례에서 시행되어 이중 2례만 구제되었다. 여성이거나 거대 종격동 림프선비대가 있는 경우에 재발이 의미있게 많았고 전체 생존율은 B 증상이 있거나 임상적 병기가 높을수록 의미있게 낮았다. EORTC 예후 인자군에 따른 5년 무병 생존율은 very favorable군(VF군), favorable군(F군), unfavorable군(U군)이 각각 100, 100, 55.8%, 5년 전체 생존율은 100, 100, 75.1%로 통계적으로 의미있는 차이를 보여주었다. VF군과 F군은 방사선치료만으로도 100%의 생존율을 보였으나 U군에서는 항암화학방사선병용요법시 방사선치료 단독보다 적은 재발율을 보였으나 생존율은 비슷하였다. 방사선치료를 받았던 경우에 맨틀방사선치료보다 부분적 림프절방사선치료를 받은 경우에 무병 생존율이 높았다. 결 론 : 횡경막상부에 국한된 호지킨병에서 시험개복술을 시행하지 않고도 좋은 치료 결과를 보여주었다. 성별, 거대 종격동 림프선비대가 무병 생존율에 의미있는 영향을 주었고 B 증상, 임상적병기는 전체 생존율에 영향을 주는 중요한 예후 인자였다. 그리고 EORTC 예후 인자군에 따른분류가 예후 예측과 치료 방법을 결정하는데 유용함을 확인할 수 있었다. Purpose : The aim of this retrospective study is to assess the necessity of staging laparotomy in the management of supradiaphragmatic CS Ⅰ- Ⅱ Hodgkin's disease. Prognostic factors and the usefulness of prognostic factor groups were also analyzed. Materials and Methods : From 1985 to 1995, fifty one patients who were diagnosed as supradiaphragmatic CS Ⅰ- Ⅱ Hodgkin's disease at Yonsei Cancer Center in Seoul, Korea were enrolled in this study. Age range was 4 to 67 with median age of 30. The number of patients with each CS ⅠA, ⅡA, and ⅡB were 16, 25, and 10, respectively. Radiotherapy(RT) was delivered using 4 or 6 MV photon beam to a total dose of 19.5 to 55.6Gy (median dose : 45Gy) with a 1.5 to 1.8Gy per fraction. Chemotherapy(CT) was given in 2-12 cycles(median : 6 cycles). Thirty one patients were treated with RT alone, 4 patients with CT alone and 16 patients with combined chemoradiotherapy. RT volumes varied from involved fields(3), subtotal nodal fields(18) or mantle fields(26). Results : Five-year disease-free survival rate(DFS) was 78.0% and overall survival rate(OS) was 87.6%. Fifty patients achieved a complete remission after initial t reatment and 8 patients were relapsed. Salvage therapy was given to 7 patients, 1 with RT alone, 4 with CT alone, 2 with RT + CT. Only two patients were successfully salvaged. Feminine gender and large mediastinal adenopathy were significant adverse prognost ic factors in the univariate analysis for DFS. The significant adverse prognostic factors of OS were B symptom and clinical stage. When patients were analyzed according to European Organization for Research and Treatment of Cancer(EORTC) prog-nostic factor groups, the DFS in patients with very favorable, favorable and unfavorable group was 100, 100 and 55.8%(p<0.05), and the OS in each patients' group was 100, 100 and 75.1%(p<0.05), respectively. In very favorable and favorable groups, the DFS and OS were all 100% by RT alone, but in unfavorable group, RT with CT had a lesser relapse rate than RT alone. The subtotal nodal irradiation had better DFS than mantle RT in patients treated with RT. Conclusion : In present study, the DFS and OS in patients who did not undergo staging laparotomy were similar with the results in the literatures of which patients were surgically staged. Therefore, we may suggest that staging laparotomy would not influence the outcome of treatments. In univariate analysis, gender, large mediastinal adenopathy, B symptoms and clinical stage were significant prognostic factors for the survival rate. We confirm the usefulness of EORTC prognostic factor groups which may be a good indicator to select the treatment modality.