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황하선,공동수,신동석,전지홍 한국물환경학회 2004 한국물환경학회지 Vol.20 No.6
Field experimental study was performed to examine characteristics of nutrient export from paddy rice fields with irrigation practices. Experimental fields with surface-water and ground-water irrigation were monitored and analyzed during rice culture period. The water balance showed that outflow generally balanced the inflow showing that about half (58∼68%) of total outflow was lost by surface drainage. Water and nutrient export are more in surface-water irrigation paddy than in ground-water irrigation paddy. The reasons might be more irrigation water available and easy to use in surface-water irrigation. If irrigation water reduced, it could result in reduction of nutrient export in paddy rice fields, which can save water and protect water quality. However, deviation from conventional standard practices might affect the rice yield and further investigations are necessary.
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현경선,이향련,공송심,윤경자,김현섭,김효남,최지원,김운정 성인간호학회 2001 성인간호학회지 Vol.13 No.2
The purpose of this study was to investigate the effect of a hand massage program on anxiety, vital sign and pain in clients with ischemic heart disease. The design utilized for this stuffy was quail-experimental with a nonequivalent control group non-synchronized design The subjects were fifty-four patients, twenty-eight for the experimental and twenty-six for the control group, who were admitted with ischemic heart diease at a cardiac intensive care unfit in K medical center of K university. This study was carried out from May, 1999 to March, 2000. The level of anxiety and pain measured by Visual Analogue Scale, systolic blood pressure, diastolic blood pressure and pulse rate were measured before and after hand massage, the state of Anxiety was measured by the Spielberger (1970) scale at admission and after hand massage for three days. The collected data were processed by using the SPSS PC program and analyzed using χ2-test and t-test. The result of this study are as follows : 1. The scores of VAS anxiety and State of anxiety of the experimental group were lower than those of the control group. 2. The degree of systolic blond pressure, diastolic blood pressure and pulse rate of the experimental group were lower than that of the control group. 3. The score of VAS pain of the experimental group was lower than that of the control group. The results suggested that hand massage can decrease VAS anxiety, State of anxiety, vital signs and VAS pain of patients who were admitted with ischemic heart disease at cardiac intensive care unit Therefore, It Is proposed that hand massage is an appropriate nursing intervention to relieve anxiety of the patients who were admitted with ischemic heart disease at a cardiac intensive care unit.
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Pathogenesis, Early Diagnosis, and Therapeutic Management of Alcoholic Liver Disease
Kong, Ling-Zu,Chandimali, Nisansala,Han, Ying-Hao,Lee, Dong-Ho,Kim, Ji-Su,Kim, Sun-Uk,Kim, Tae-Don,Jeong, Dong Kee,Sun, Hu-Nan,Lee, Dong Sun,Kwon, Taeho MDPI AG 2019 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.20 No.11
<P>Alcoholic liver disease (ALD) refers to the damages to the liver and its functions due to alcohol overconsumption. It consists of fatty liver/steatosis, alcoholic hepatitis, steatohepatitis, chronic hepatitis with liver fibrosis or cirrhosis, and hepatocellular carcinoma. However, the mechanisms behind the pathogenesis of alcoholic liver disease are extremely complicated due to the involvement of immune cells, adipose tissues, and genetic diversity. Clinically, the diagnosis of ALD is not yet well developed. Therefore, the number of patients in advanced stages has increased due to the failure of proper early detection and treatment. At present, abstinence and nutritional therapy remain the conventional therapeutic interventions for ALD. Moreover, the therapies which target the TNF receptor superfamily, hormones, antioxidant signals, and MicroRNAs are used as treatments for ALD. In particular, mesenchymal stem cells (MSCs) are gaining attention as a potential therapeutic target of ALD. Therefore, in this review, we have summarized the current understandings of the pathogenesis and diagnosis of ALD. Moreover, we also discuss the various existing treatment strategies while focusing on promising therapeutic approaches for ALD.</P>
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A case of Castleman disease presented as pelvic mass
( Ji Sun Lee ),( Yong Hee Lee ),( Tae Wook Kong ),( Suk Joon Chang ),( Ki Hong Chang ),( Hee Sug Ryu ),( Ji Heum Paek ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.98 No.-
Castleman disease is giant lymph node hyperplasia and is a rare cause of lymph node enlargement. In this study, we report a case of a 29-year-old woman with abnormal pelvic mass. Preoperative transvaginal sonography initially suggested benign neoplasm of right ovary and additional abdomen CT indicated abnormal enhancing mass on right extraperitoneal pelvis. Surgical exploration revealed about 5cm sized mass attached to the right pelvic peritoneum. The mass was removed by laparoscopic resection. Pathological investigation confirmed hyaline vascular type of Castleman disease. The possibility of Castleman disease should be considered when assessing a pelvic mass. The specific imaging findings and review of disease are discussed.
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( Ji Sun Lee ),( Seong Hye Gweon ),( Suk Joon Chang ),( Tae Wook Kong ),( Ji Heum Paek ),( Hee Sug Ryu ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-
목적: The purpose of this study was to evaluate the predicting model for lymph node metastasis using preoperative tumor grade, transvaginal ultrasound (TVS), and serum CA-125 level in patients with endometrial cancer. 방법: Between January 2000 and February 2013, we identified 172 consecutive patients with surgically staged endometrial cancer. TVS was performed by an expert gynecologic radiologist in all patients. All patients had complete staging surgery including bilateral pelvic and para-aortic lymphadenectomy and were staged according to the 2009 FIGO classification. Various clinico-pathological data were obtained from medical records and retrospectively analyzed. 결과: Of 172 patients, 138 patients presented with stage I (118 IA, 20 IB), 12 had stage II, 18 had stage III (2 IIIA, 1 IIIB, 8 IIIC1, 7 IIIC2), and 2 had stage IV diseases. The majority of patients had endometrioid adenocarcinoma (88.4%) and others (12.6%) had non- endometrioid histology. Eighteen patients (10.5%) were found to have lymph node metastasis. Deep (≥50%) myometrial invasion on preoperative TVS, high serum CA-125 level (≥ 35 IU/mL), preoperative grade 3 tumors were significant preoperative factors predicting lymph node metastasis. There was no significant association between preoperative histology and lymph node metastasis. We calculated the simple model predicting lymph node metastasis based on preoperative tumor grade, TVS findings, and CA-125 level using logistic regression analysis. With the cut-off point of 1.5, the sensitivity and specificity of this model were 94% and 57%, respectively (AUC = 0.84, [95% CI, 0.74-0.93], p< 0.01). 결론: Preoperative tumor grade, myometrial invasion on preoperative TVS, and CA-125 can accurately predict lymph node metastasis in endometrial cancer patients. The current study suggests the possibility that TVS could be positively used for preoperative evaluation strategy in the low-resource countries instead of expensive imaging modalities such as MRI or PET-CT.
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( Ji Sun Lee ),( Seong Woo Kang ),( Tae Wook Kong ),( Ji Heum Paek ),( Hee Sug Ryu ),( Suk Joon Chang ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-
To determine whether pretreatment serum CYFRA 21-1 levels can be a useful prognostic indicator in cervical cancer with reference to squamous- cell-carcinoma-related antigen (SCC Ag). We retrospectively reviewed five-hundred and six cervical cancer patients treated by radical hysterectomy or primary concurrent chemoradiotherapy. Pretreatment serum SCC Ag and CYFRA 21-1 levels of 506 cervical cancer patients were measured. A multivariate analysis using Cox`s proportional hazard model was performed to evaluate the prognostic significance of pretreatment variables. There was significant relationship between pre-treatment serum SCC Ag/ CYFRA 21-1 levels and patient age, advanced FIGO stage, large tumor size, lymph node metastasis, and deep stromal invasion. Advanced FIGO stage (stage III and IV, p<0.001), large tumor size (> 4 cm, p<0.001), and elevated pretreatment SCC Ag (≥ 1.95 ng/mL, p<0.001) and CYFRA 21-1 (≥ 2.95 ng/mL, p<0.001) levels significantly decreased 5-year disease-free survival rates. In the stepwise Cox regression analysis, pretreatment serum CYFRA 21-1 (p=0.015) levels was the only independent prognostic factor for disease-free survival and SCC Ag levels showed marginal significance (p=0.053). Advanced FIGO stage (p<0.001), large tumor size p<0.001), age under 50 years old (p=0.037) and elevated pretreatment SCC Ag (p=0.024) and CYFRA 21-1 (p<0.001) levels significantly decreased 5-year overall survival rates. In the stepwise Cox regression analysis, advanced FIGO stage (p=0.002), large tumor size p=0.013), age under 50 years old (p=0.006) were independent prognostic factors for overall survival. Pretreatment CYFRA 21-1 levels significantly correlated with disease-free survival.
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( Ji Sun Lee ),( Seong Hye Gweon ),( Tae Wook Kong ),( Ji Yoon Cheong ),( Jeong In Yang ),( Haeng Soo Kim ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-
목적: The aim of present study was to evaluate the perinatal outcomes of selective intrauterine growth restriction (sIUGR) and non-selective IUGR (non-sIUGR) in twin pregnancy. 방법: This was a retrospective study of twin pregnancies from 2007 to 2014. A total number of 385 pairs of twin born between 2007 and 2014 at Ajou University Hospital were enrolled in the study. According to the birth weight, the 385 pairs of twins were divided into sIUGR, non-sIUGR, and normal growth groups. Maternal characteristics and perinatal outcomes were compared among three groups. 결과: Among 385 twin pregnancies, 80 (20.8%) were sIUGR, 15 (3.9%) were non-sIUGR, and 290 (75.3%) were normal growth group. There was no significant difference between IUGR and sIUGR groups according to chorionicity (p=0.417). Vaginal delivery was significantly higher in non-sIUGR group compared to sIUGR and normal growth groups (46.7% vs. 16.2% vs. 14.5%, p=0.014). The incidence of fetal distress and chorioamnionitis were higher in the non-sIUGR group than in sIUGR and normal growth groups (fetal distress: 20.0% vs. 2.5% vs. 1.4% , p<0.001, chorioamnionitis: 53.0% vs. 30.0% vs. 21.7%, p=0.004). Maternal preeclampsia was higher in IUGR group (sIUGR and non-sIUGR) than in normal growth group (18.8% vs. 26.7% vs. 9.3%, p=0.004). With respect to perinatal outcomes, perinatal death was higher in sIUGR group than in normal growth group (10.6% vs. 4.8%, p=0.006). The incidence of poor perinatal outcome (any of the followings: NEC, BPD, RDS, and brain hemorrhage) was higher in both s-IUGR and non-sIUGR groups than in normal growth group (17.6% vs. 23.3% vs. 11.3%, p=0.007). 결론: There was significant difference between sIUGR and non-sIUGR groups in terms of vaginal delivery, fetal distress, and chorioamnionitis. Poor perinatal outcome was higher in both non-sIUGR and sIUGR groups compared to normal growth group.
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Ji-Yoon Hwang,Myeong-Don Joo,Hongyu Liu,Muhamad Idress,Sun-Hwa Oh,Il-Keun Kong 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11
Mammalian fetal ovaries contains numerous primordial germ cells, however fewer ones can yield mature oocytes due to apoptosis and follicle atresia. Successful in vitro reconstitution of primordial germ cells has recently had a significant effect in the field of assisted reproductive technologies. However, the regulatory mechanisms underlying oogenesis remain unknown and recapitulation of oogenesis in vitro remains unachieved. Therefore, development of methods for obtaining mature oocytes by culturing the fetal ovaries in vitro could contribute to clarify these mechanisms. We adapt an in vitro system for culturing mouse fetal ovaries that support successful follicle assembly and improve oocyte growth and maturation. Ovarian tissues from 12.5 days postcoitum (dpc) fetal mice were cultured in vitro and the matured oocytes were differentiated from primordial germ cells after a 31 days culture period. Our results demonstrate that mouse fetal germ cells are able to form primordial follicles with artificial ovarian cells, and that oocytes within the growing follicles are able to mature normally in vitro. Taken together, this in vitro culture system is expected to aid in the development of new strategies to identify the reasons behind failure of follicle assembly and offer a platform for innovative research into preservation of female germ cells and conservation of endangered species.
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Kong, Byung Ho,Shin, Hyun-Do,Kim, Se-Hoon,Mok, Hyun-Su,Shim, Jin-Kyoung,Lee, Ji-Hyun,Shin, Hye-Jin,Huh, Yong-Min,Kim, Eui-Hyun,Park, Eun-Kyung,Chang, Jong Hee,Kim, Dong-Seok,Hong, Yong-Kil,Kim, Sun Ho Lychnia 2013 International journal of oncology Vol.42 No.5
<P>The presence of glioma stromal mesenchymal stem?like cells (GS-MSLCs) in tumors from glioma patients has been previously reported. The mechanisms through which these cells function as a part of the glioma microenvironment, however, remain incompletely understood. We investigated the biological effects of GS-MSLCs on glioma cancer stem cells (gCSCs), testing the hypothesis that GS-MSLCs alter the biological characteristics of gCSCs. GS-MSLCs and gCSCs were isolated from different glioblastoma (GBM) specimens obtained from patients. In in vitro experiments, gCSCs were cultured alone or co-cultured with GS-MSLCs, and gCSCs cell counts were compared between the two groups. In addition, two groups of orthotopic GBM xenografts in mice were created, one using gCSCs from the monoculture group and one using gCSCs isolated from the co-culture group, and tumor volume and survival were analyzed. Furthermore, in vivo proliferation, apoptosis and vessel formation were examined using immunohistochemical analyses. In vitro cell counts for gCSCs co-cultured with GS-MSLCs increased 3-fold compared to gCSCs cultured alone. In orthotopic xenograft experiments, mice injected with gCSCs isolated from the co-culture group had significantly larger tumor volume, measured on day 40 after injection, and their survival times were shorter. Immunohistochemical analysis showed increased tumor expression of CD31, indicative of enhanced microvessel formation in mice injected with gCSCs co-cultured with GS-MSLCs compared to mice injected with gCSCs cultured alone. However, proliferation (PCNA) and apoptosis (TUNEL) markers showed no significant difference between the two groups. In conclusion, GS-MSLCs may influence the biological properties of gCSCs, shifting them towards a more aggressive status; moreover, increased angiogenesis may be a critical component of this mechanism.</P>
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