잠김 금속판을 이용한 대퇴골 전자하 골절의 치료 : 압박 고 나사와 비교

손현철, 김용민, 김동수, 최의성, 박경진, 조병기, 박지강, 배승환 충북대학교 의과대학 충북대학교 의학연구소 2011 忠北醫大學術誌 Vol.21 No.2

-본문참고-

매실 추출물을 함유한 음료가 식중독 유발균의 성장에 미치는 영향

배지현,김기진 동아시아식생활학회 1999 동아시아식생활학회지 Vol.9 No.2

Prunus mume has been used for the folk medicine by many old civilizations to treat food-borne diseases or enteric disorders. The purpose of this study was to investigate the antimicrobial activity of beverages containing Prunus mume extracts against Staphylococcus aureus, Shigella dysenteriae, Shigella flexneri, Shigella sonnei, Escherichia coli and Pseudomonas aeruginosa. Seven different types of Prunus mume extracts containing beverages have been prepared for the test in which minimum inhibitory concentration for each microorganism has been determined by serial dilution method using either TSA or TSB medium. Pseudomonas aeruginosa showed least resistance and 0.3g/㎖ concentrations of 5% Prunus mume extracts containing beverage had antimicrobial property against the organism. Beverages containing more than 15% of Prunus mume extracts showed antimicrobial activity against all tested microorganisms at the MIC value of less than 0.25g/㎖. The effect of Prunus mume on the growth of food-borne pathogens has been also studied using a spectrophotometer. In the growth assay, each of the Prunus mume extracts containing beverage was added to the medium at the concentration of 25% (v/v). Beverage containing 20% Prunus mume extracts showed inhibitory effect on the growth of all tested microorganisms.

고농도 삼투환경에서 생성되는 포도상구균의 Proline과 Glycine Betaine Transport System의 수준변화에 관한 연구

배지현 동아시아식생활학회 1995 동아시아식생활학회지 Vol.5 No.2

식품에 오염되어 식중독을 유발하는 균인 포도상구균은 수분활성치가 0.90이하인 경우에서도 자랄 수 있다는 점이 다른 food-borne pathogens와는 구분된다. 이는 proline 이나 glycine beraine과 같은 compatible solutes가 transport에 의해 세포내에 축적되어 삼투압이 조절되기 때문이다. 본 연구에서는 이 세포가 높은 농도의 삼투환경에서 자랄 때 proline transport ststems와 glycine betaine transport system의 level이 어떻게 변화하는지에 대해 조사해 보았다. 세포를 삼투압이 높은 환경에서 자라게 했을 때 이들 ransport systems의 level은 증가하였고 특히 low-affinity proline ransport system의 증가가 가장 현저하였다. 그러나 S. aureus가 고농도 삼투환경에서 자랐다 할지라도 5mM glycine betaine을 성장배지에 넣어 주었을 경우 low-affinity proline ransport system의 level은 증가하지 않았다. 세포내 축적된 proline과 glycine betaine이 어떻게 변하는지 thin-layer chromatography로 분석해 본 결과, 이들은 세포내에서 다른 물질로 전환되지 않았다. Staphylococcus aureus, the most salt-tolerant nonhalophilic bacterium, is the only foodborne pathogen that is able to grow at a_(w) levels below 0.90. The fundamental osmoregulatory strategy used by this organism involves the accumulation of intracellular compatible solutes such as proline or glycine betaine which are accumulated by transport and act as osmoregulators in cells. In this study, levels of proline transport systems and glycine betaine transport system of S. aureus were examined when cells are grown at high osmolarity. The levels of all three transport systems within S. aureus were elevated at high osmolarity and the most dramatic increase was found for the low-affinity proline transport system. However, in 5mM glycine betaine-supplemented medium, the level of the low-affinity proline transport system did not become elevated when cultures were grown at high osmolarity. The metabolic fate of the accumulated proline and glycine betaine was investigated by thin-layer chromatography and found to be not metabolized by S. aureus.

직접치수복조재에 따른 비글견 치수의 조직반응에 대한 연구

배지현,김영균,윤필영,조병훈,최용훈 大韓齒科保存學會 2010 Restorative Dentistry & Endodontics Vol.35 No.1

본 연구에서는 인위적으로 노출시킨 비글견의 치수조직을 기존의 수산화칼슘제재와 MTA, 접착성 레진, 광중합형 수산화칼슘제재를 사용하여 직접치수복조한 후 각 재료에 따른 치수의 반응을 광학현미경 하에서 조직학적으로 관찰하여 비교분석하였다. 2마리 비글견의 36개 치아를 이용하여, 실험적으로 치수를 노출시키고 노출된 치수에 치수복조재를 적용한 후 와동은 복합레진으로 충전하여 직접치수복조술을 시행하였다: (1) Mineral Trioxide Aggregate (MTA: ProRoot MTA, Dentsply, Tulsa, USA), (2) Clearfil SE Bond (Dentin adhesive system: Kuraray, Osaka, Japan), (3) Ultra-Blend (Photopolymerized Calcium hydroxide: Ultradent, South Jordan, USA), (4) Dycal (Quick setting Calcium hydroxide: LD Caulk Co., Milford, USA). 희생전 90일, 30일, 7일 전에 각 복조재별로 3개씩의 와동을 충전하였고 비글견을 희생시키고 조직시편을 제작하였다. 시편을 H&E 염색 후 광학 현미경으로 치수염증반응과 경조직 형성 정도를 관찰하였다. MTA 군은 초기에는 경조직 형성이 관찰되지 않고 치수조직의 위축과 부분괴사가 관찰되었으나 시간이 지나면서 경조직 형성이 관찰되었다. Clearfil SE Bond군은 초기에 염증세포의 침윤과 치수세포의 괴사를 관찰할 수 있었고 시간이 지날수록 치수 세포의 괴사가 더욱 진행한 양상을 보였다. Ultra-blend 군과 Dycal 군은 MTA 군과 비슷하게 초기에는 경조직 형성을 보이지 않고 중등도의 염증반응이 관찰되었으며 시간이 지나면서 경조직 형성이 관찰되었다. MTA, 수산화칼슘제재와 광중합 수산화칼슘제재는 초기의 치수염증반응 이후 조상아세포층, 상아질교 형성을 보여 직접치수복조재로 적당하였으며, 접착성 레진은 심한 염증반응과 치수 조직의 괴사 양상을 보여 직접치수복조재로 부적당하다는 결론을 얻을 수 있었다. The purpose of this study was to evaluate the pulp tissue reaction to direct pulp capping of mechanically exposed beagle dogs' pulp with several capping materials. A total of 36 teeth of 2 healthy beagle dongs were used. The mechanically exposed pulps were capped with one of the followings: (1) Mineral Trioxide Aggregate (MTA: ProRoot MTA. Dentsply, Tulsa, USA), (2) Clearfil SE Bond (Dentin adhesive system: Kuraray, Osaka, Japan), (3) Ultra-Blend (Photo-polymerized Calcium hydroxide: Ultradent, South Jordan, USA), (4) Dycal (Quick setting Calcium hydroxide: LD Caulk Co., Milford, USA) at 7, 30, and 90 days before sacrificing. The cavities were restored with Z350 flowable composite resin (3M ESPE, St. Paul. MN, USA). After the beagle dogs were sacrificed, the extracted teeth were fixed, decalcified, prepared for histological examination and stained with HE stain. The pulpal tissue responses to direct pulp capping materials were assessed. In MTA calcium hydroxide, and photo-polymerized calcium hydroxide groups, initial mild inflammatory cell infiltration, newly formed odontoblast-like cell layer and hard tissue bridge formation were observed. Compared with dentin adhesive system, these materials were biocompatible and good for pulp tissue regeneration. In dentin adhesive system group, severe inflammatory cell infiltration, pulp tissue degeneration and pulp tissue necrosis were observed. It seemed evident that application of dentin adhesive system in direct pulp capping of beagle dog teeth cannot lead to acceptable repair of the pulp tissue with dentine bridge formation.

정호승의 시 연구 : 90년대 후기 시편을 중심으로

지현배 慶北大學校 出版部 2002 人文 科學 Vol.16 No.-

In the Intellectual History of the Kyongpuk Province it is so valuable to estimate the poets in this region not only in the matter of quantity but of quality. The study of Chong Ho-Seung, who was a poet innate from kyongpuk Province, therefore, is likely to reveal some critical and literary features of the kyungpuk Province. Chong Ho-Seung`s poems produced in 1990`s is thought to apply the variation which leads to the new understading of the poems. In my studies which seeks the characteristic of the prose in the poems, I found it also broughts the combination of lyrical effects. In addition, the close observance of Chong`s works enable us to find that his word selection in the poems serves as the factor not only to configure its main theme but to refresh the responses of the readers. To analyze the poet as a human is the process with warmth and beauty from the beginning. And I think, though in the contrary, the words becomes the beauty and the warmth through the poet.

비파괴적 분석법을 활용한 활음진 함유 에멀젼의 인체효능평가

배지현,안성연,이해광,안순애,문성준,장이섭 한국비파괴검사학회 2004 학술대회 논문집 Vol.- No.-

최근 한방화장품이 각광을 받으면서 한방에서 말하는 피부개선효과에 대한 적절한 평가법을 찾는 연구가 많이 수행되고 있다. 본 연구에서는 피부 항산화 효과가 우수한 산사자, 당귀, 홍화, 두충, 숙지황의 초임계추출물 (활음진, 活陰眞)의 혈액순환 개선효과를 적외선 열화상 이미지 분석 및 LaserDoppler Perfusion Imager를 이용한 분석을 통해 평가하였다. 평소 손발이 차가운 여성 11명을 대상으로 활음진을 포함한 에멀젼을 1주일간 도포하게 하였으며, 도포전과 1주일간 사용한 후 피부 온도와 혈류량을 비교하였다. 그 결과 얼굴의 피부 온도가 증가하였고, 이마, 눈위, 뺨정면 부위에서 시료 사용 후 유의한 증가를 보였다. 본 연구를 통하여 활음진 성분을 포함한 에멀젼의 혈액순환 개선효과를 확인하였을 뿐만 아니라 한방적 접근을 통한 피부개선효과를 평가하는데 적절한 실험모델을 수립하였다.

청피 추출물이 식중독 유발 미생물의 증식에 미치는 영향

배지현,박효은,배희정 한국조리과학회 2005 한국식품조리과학회지 Vol.21 No.1

This study was performed to investigate the antimicrobial effects of the Citrus unshiu Markovich extracts against food-borne pathogens. First, the Citrus unshiu Markovich was extracted with methanol at room tempetsture, and fractionation of the methanol extracts from Citrus unshiu Markovich was carried out by using petroleum ether, chloroform, ethyl acetate, and methanol. The antimicrobial activity of the Citrus unshiu Markovich extracts was determined using the paper disc method against food-borne pathogens and food spoilage bacteria. The ethyl acetate extracts ofCitrus unshiu Markovich showed the highest antimicrobial activity against Bacillus cereus and Shigella sonnei. A synergistic effect was found in combined extracts of Citrus unshiu Markovich and ffedyotis diffusa Willd as compared to each extract alone.Finally. the growth inhibition curve was determined using ethyl acetate extracts of Citrus unshiu Markovich against Bacillus cereus and Shigella simnel. The ethyl acetate extract of Citrus unshiu Markovich showed strong antimicrobial activity against Bacillus cereus at due concentration of 5,000 ppm. The 5.000 ppm of ethyl acetate extract from Citrus unshiu Markovich retarded the growth of Bacillus cereus more than 24 hours and Shigella sonnet up to 24 hours. The ethyl acetate extracts of Citrus unshiu Markovich have shown an antimicrobial effect against Bacillus cereus and Shigella sonnei.

수준별 수영 참여자의 동기유형 분석

배병석,정재은,육동원,김지수,김민현 한국스포츠리서치 2005 한국 스포츠 리서치 Vol.16 No.2

The purpose of this study was to examine motivation type of swimmers by competency level. A total of 262 swimmers were composed of 111 beginners, 58 inter-mediates and 93 advancers participated in the study. For the study, duration of participation, frequency of participation and the level of sport performance of participants were set as the independent variable, while motivation type was the dependent variable. The study used analysis methods such as frequency analysis, t-test, one-way ANOVA between the independent and dependent variables. A significant difference was found after conducting an analysis on the difference in motivation type by duration of participation. The analysis on the difference in motivation type by frequency of participation showed that a group that swam three to five times per week recorded higher marks in terms of physical condition and social life factors than that swam two to three times per week. The analysis on the difference in the motivation type by level of sport performance of participants showed a significant difference in development of skills, enjoyment, achievement, physical condition. social life. external ostentation and amotivation factors.

상아질 접착제의 중합 시간 조절에 따른 복합레진의 중합 수축 방향의 변화

배지현,오명환,김창근,손호현,엄정문,조병훈,권혁춘 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.4

The purpose of this study was to evaluate the effect of adhesive curing timing on the direction of polymerization shrinkage of light-curing composite resin. In this study, the curing times of adhesive and composite resin were measured by differential scanning calorimeter(DSC). 28 extracted human molars were embedded in clear resin and box-type cavities were prepared. Based on DSC data. the experimental teeth were divided into 4 groups. Group 1: no bond: Group 2: late curing: Group 3: Intermediate curing: Group 4: Early curing. After treating with adhesive, the buccal cavities were filled with Z-100 hybrid composite resin and the lingual ones were filled with AEliteflo flowable composite resin. The depressions at the surface were measured by surface profilometer, then the specimens were embedded in clear resin and sectioned. Impressions were obtained and used to get epoxy resin replicas. The epoxy replicas were gold-coated and observed under SEM. Average Maximum Gap(AMG), Gap Proportion(GP), Average Marginal Index(AMI) were used to compare the shrinkage gap of each group. The results were statistically analyzed using the Kruskal-Wallis One Way ANOVA, Student-Newman-Keuls method. The results of this study were as follows. 1. Average Maximum Gap. Gap Proportion, Average Marginal Index and depression at the surface of Z-100 hybride composite resin were smailer than those of AEliteflo flowable composite resin(P(0.05) 2. When the bonding between composite resin and tooth structure was strong, the shrinkage gap was small, and depression at the surface was deep(P<0.05). 3. In the well-bonded group. light-curing composite resin shrank toward bonded cavity wall. not toward light source. The result suggested that the direction of polumerization shrinkage was affected by the quality of bonding in the dentin-resin interface. The strong was the bonding between composite resin and tooth structure, the smaller was the gap and the deeper was the depression at the surface. Then the flow to compensate the polymerization shrinkage proceeded from surface to bonded cavity wall.

한국판 주의력결핍 장애 평가 척도-가정판: 신뢰도 및 타당도 연구

강지현,박성찬,박형배 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.6

저자들은 주의력결핍 과잉운동장애에 대한 부모 평가척도인 주의력결핍 장애 평가척도-가정판(ADDES-HV)의 한국판 개발을 위해 본 연구를 시행하였다. 대구시 지역에 소재한 3개 국민학교 2학년 아동 538명과 주의력결핍 과잉운동장애 환자 36명의 환자군을 대상으로 ADDES-HV의 신뢰도와 타당도에 관한 검증을 실시하여 다음과 같은 결론을 얻었다. 1) 세 학교사이의 ADDES-HV 점수는 차이가 없었다. 2) 세 학교 학생들의 남녀간 비교에서 남자가 ADDES-HV 소척도 및 전체 점수 모두에서 여자보다 유의하게 높았다(p<0.05). 3) 주의력결핍 과잉운동장애 환자군 및 정상 대조군에서 남녀간의 ADDES-HV의 점수에는 유의한 차이가 없었다. 4) 검사-재검사 신뢰도 검증에서 신뢰도 계수는 주의 산만이 0.80, 충동성이 0.69, 과잉 운동이 0.63, 전체 점수 0.82로 모두 만족할 만큼 높았다 (p<0.05). 5) 내적일관성에 의한 신뢰도 검증에서 Cronbach α값은 주의산만,충동성,과잉운동 소척도에서 각각 0.91, 0.88, 0.89(p<0.05)이었고, 반복신뢰도 계수는 주의산만, 충동성, 과잉운동 소척도에서 각각 0.86, 0.86, 0.80(p<0.05)이었다. 6) ADDES-HV와 DSM-Ⅲ-R 척도와의 공존 타당도는 주의력결핍 과잉운동장애 환자군에서 0.57(p<0.05), 정상 대조군에서 0.84(p<0.05)였다. 7) 주의력결핍 과잉운동장애 환자군과 정상 대조군간의 감별 타당도 검증에서 모두 유의하게 환자군이 높은 점수를 보였다(p<0.05). 8) ADDES-HV의 환자군에 대한 판별력을 평가하기 위한 판별 분석에서 전체 판별력은 94.44%였다. 9) 정상적인 점수의 상한선인 절단점은 표준편차 1.5로 볼 때, 국민학교 2학년 남학생은 80점, 여학생은 69점이었다. 따라서 ADDES-HV 척도를 한국인에 적용한 결과 신뢰롭고 타당함이 입증되었고 환자를 판별하는 능력이 우수하여 향후 주의력결핍 과잉운동장애에 대한 선별 검사 및 조사 연구에 유용한 도구임이 입증되었다. The purpose of this study was to examine the reliability and validity of a Korean version of Attention Deficit Disorder Evaluation Scale-Home Version(ADDES-HV). ADDES-HV was administered to 538 second grade students from 3 elementary schools and 36 Attention Deficit Hyperactivity Disorder patients. There were no differences in the mean scores of ADDES-HV among schools. Male students showed significantly higher scores than female students in both subscale and total scores(p<0.05). There were no significantly differences in ADDES-HV scale scores between male students and female students both in attention deficit hyperactivity disorder patients group and in normal control group. In the reliability test, the test-retest reliability coefficient 0.82, significantly high ; 0.80 for inattention, 0.69 for impulsivity, 0.63 for hyperactivity and total score was 0.82. In the reliability test by internal consistancy, the Cronbach αcoefficient was also high ; 0.91 for inattention, 0.88 for impulsivity, 0.88 for hyperactivity(p<0.05). The split-half reliability coefficients by first-second half method showed high correlation, and coefficient of inattention was 0.86, that of impulsivity 0.86, that of hyperactivity 0.80(p<0.05). The concurrent validity between ADDES-HV and DSM-Ⅲ-R scale was 0.57 in attention deficit hyperactivity disorder patient group and 0.84 in normal control group(p<0.05). The ADHD group was were more discriminated than control group by ADDES-HV(p<0.05). The total discriminant capacity of the patient group in ADDES-HV was 94.44%. Regarding the cutoff point as 1.5 standard deviations, the cutoff scores were 80 for male students and 69 for female students. In this point of view, ADDES-HV scale showed high reliability and validity in applying to Korean subjects and was proved to be the useful screening test tool for attention deficit hyperactivity disorder research.