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Enhanced Spectral Resolution of Multiplex CARS Microscopy with a Spectral Beam Shaper
Sae Chae Jeoung,최대식,윤태오 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.2
Enhancement of the spectral resolution in multiplex coherent anti-Stokes Raman scatering (CARS) microscopy is achieved by adapting a spectral beam shaper, which can vary the spectral bandwidth of the pump beam from 11 cm-1 to 28 cm-1. The feasibility of this way to atain high spectral resolution in multiplex CARS microscopy was tested on polyviny lthin films coated on slide glas and liquid 1,3,5-Trisopropylbenzene. The best spectral resolutions of about 24 cm-1 and 18 cm-1 for the C-H vibration mode of solid polymeric thin films and liquid 1,3,5-Trisopropylbenzene, respectively, show a potential applicability of the multiplex configuration in CARS microscopy to solid polymer thin films and to liquid samples with rather complex vibrational spectra.
Visualization of Bonghan Microcells by Electron and Atomic Force Microscopy
백구연,오가이,Sae Chae Jeoung,소광섭 사단법인약침학회 2009 Journal of Acupuncture & Meridian Studies Vol.2 No.2
Abstract Objectives: The origin of adult stem cells remains an open question. If they derive from embryos, it is difficult to determine the mechanism which interrupts their differentiation during tissue formation. In the 1960s, the Bonghan microcell was suggested as one possible, yet to be described, route of stem cell production, such that they have the potential to proliferate to produce normal cells. Materials and Methods: In this study, Bonghan microcells were isolated from Bonghan tissues on rat organ surfaces, and their detailed morphology examined by electron and atomic force microscopy. Results: The ultrastructure observed distinguished them from apoptotic bodies and other microorganisms, and their unique, possible proliferation feature, as protruding threads, was imaged by atomic force microscopy. Conclusions: The unique threadlike structure of the Bonghan microcell is consistent with Prof. Kim's observation in the first step of making a cell. Understanding of the functions of this threadlike structure may give a clue to understand the origin or the differentiation cue of adult stem cells.
정세채,이흥순,시두,문혜영,Jeoung, Sae Chae,Lee, Heung Soon,Sidhu, M.S.,Moon, Heh-Young 한국레이저가공학회 2014 한국레이저가공학회지 Vol.17 No.4
Femtosecond laser induced shock generation in water and vitreous humor of enucleated porcine eyeball was investigated. When focusing the femtosecond laser into the liquid mediums, the acoustic waves with a frequency of about 15.6kHz could be observed by using wide-band microphone. The amplitude of the acoustic signals from water has attained a maximum under a laser power of about 5mW. Further increment of the power results in a decrement of the acoustic signals due to nonlinear optical process including filamentation of laser beam. We have further investigated the effect of femtosecond laser induced acoustic waves by applying the laser pulse into enucleated porcine eyeball. The comparative studies on both healthy and diseased eyeballs led us propose that the femtosecond laser pulses could be utilized as a novel tools for treatment of partially detached retina layers from their choroid structures.
Cho, Sung Mi,Jeoung, Sae Chae,Song, Ji-Young,Kupriyanova, Elena V.,Pronina, Natalia A.,Lee, Bong-Woo,Jo, Seong-Whan,Park, Beom-Seok,Choi, Sang-Bong,Song, Ji-Joon,Park, Youn-Il American Society for Biochemistry and Molecular Bi 2015 The Journal of biological chemistry Vol.290 No.47
<P>Cyanobacteriochromes (CBCRs), which are exclusive to and widespread among cyanobacteria, are photoproteins that sense the entire range of near-UV and visible light. CBCRs are related to the red/far-red phytochromes that utilize linear tetrapyrrole (bilin) chromophores. Best characterized from the unicellular cyanobacterium <I>Synechocystis</I> sp. PCC 6803 and the multicellular heterocyst forming filamentous cyanobacteria <I>Nostoc punctiforme</I> ATCC 29133 and <I>Anabaena</I> sp. PCC 7120, CBCRs have been poorly investigated in mat-forming, nonheterocystous cyanobacteria. In this study, we sequenced the genome of one of such species, <I>Microcoleus</I> IPPAS B353 (<I>Microcoleus</I> B353), and identified two phytochromes and seven CBCRs with one or more bilin-binding c<U>G</U>MP-specific phosphodiesterase, <U>a</U>denylyl cyclase and <U>F</U>hlA (GAF) domains. Biochemical and spectroscopic measurements of 23 purified GAF proteins from phycocyanobilin (PCB) producing recombinant <I>Escherichia coli</I> indicated that 13 of these proteins formed near-UV and visible light-absorbing covalent adducts: 10 GAFs contained PCB chromophores, whereas three contained the PCB isomer, phycoviolobilin (PVB). Furthermore, the complement of <I>Microcoleus</I> B353 CBCRs is enriched in near-UV and violet sensors, but lacks red/green and green/red CBCRs that are widely distributed in other cyanobacteria. We hypothesize that enrichment in short wavelength-absorbing CBCRs is critical for acclimation to high-light environments where this organism is found.</P>
Jung Rim Nam,임기수,Sae Chae Jeoung 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.5
The ablation threshold of polymethylmethacrylate (PMMA) thin films decreased with increasing dopant levels. We also fabricated grating structures in doped PMMA thin film, heated pure PMMA thin films by using femtosecond laser irradiation at 800 nm and measured the diffaction eciency of the grating by using a He-Ne laser.
Myung-Il Park,Sae Chae Jeoung,Chang Sook Kim,Chong-Ook Park 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.46 No.2
Ablation of germanium single crystals in air is performed with femtosecond laser pulses (150 fs, 1kHz) of 800-nm wavelength in the uence range of 0.52 26 J/cm2. Two dierent semi-logarithmic relationships between the ablation depth and the laser uence are observed. The structure of the ablated region is characterized by means of XRD, including a wide angle (/2) scan and the rocking curve of (400) plane. The ablated layer exhibits several polycrystalline diffraction patterns and double peaks in the rocking curve, which are related to structural defects. From these investigations, the higher laser uence in the second semi-logarithmic region obviously causes severe damage to the structure of the ablated zone.
Choi, Jun-Rye,Jeoung, Sae-Chae,Cho, Dae-Won Korean Chemical Society 2003 Bulletin of the Korean Chemical Society Vol.24 No.11
The photodynamics of excited-state intramolecular proton transfer reaction of 1-hydroxyanthraquinone (1-HAQ) and 1-deuterioanthraquinone was investigated in toluene with time-resolved emission and femtosecond transient transmittance techniques at room temperature. The temporal profiles of transient transmittance of 1-HAQ could be well described with multi-decaying time constants. The ultrafast time constant within ca. 260 fs reflects the dynamics of proton transfer. The decay component of 2 ps is assigned to an additional proton translocation process induced by the intramolecular vibrational relaxation, whereas the decay component of 18 ps is assigned to the vibrational cooling process, while the long component (200 ps) can be explained in terms of the relaxation from excited-state keto-tautomer to its ground state. Time-resolved anisotropy decay dynamics and isotope effects on the photodynamics reveals that the ESIPT from enol-tautomer to keto-one of 1-HAQ is barrierless reaction and coupled to a vibrational relaxation process.
Nam, Jung Rim,Kim, Chang Ho,Jeoung, Sae Chae,Lim, Ki Soo,Kim, Hwan Myung,Jeon, Seung-Joon,Cho, Bong Rae Elsevier 2006 Chemical physics letters Vol.427 No.1
<P><B>Graphical abstract</B></P><P>Measurement of two-photon absorption coefficient of dye molecules based on fs-laser ablation threshold <I>F</I><SUB><I>th</I></SUB></P><ce:figure id='figure.0010'></ce:figure> <P><B>Abstract</B></P><P>Two-photon absorption cross-section of a series of dyes (<B>1a</B>–<B>3a</B>) in polymer thin films was determined by measuring the femtosecond laser ablation threshold. The ablation threshold decreased gradually when the dopant increased. The two-photon absorption cross-section of the dye molecules dispersed in the polymer film was estimated by using the theoretical relationship between the ablation threshold of the blended polymeric thin films and the dye concentration. The relative values of two-photon absorption cross-section are in good agreement with those measured in solution. On the other hand, the absolute values are smaller than the latter.</P>