Overexpression of PtrMYB119, a R2R3-MYB transcription factor from Populus trichocarpa, promotes anthocyanin production in hybrid poplar

Cho, J. S.,Nguyen, V. P.,Jeon, H. W.,Kim, M. H.,Eom, S. H.,Lim, Y. J.,Kim, W. C.,Park, E. J.,Choi, Y. I.,Ko, J. H. Oxford University Press 2016 Tree physiology Vol.36 No.9

<P>Anthocyanins are a group of colorful and bioactive natural pigments with important physiological and ecological functions in plants. We found an MYB transcription factor (PtrMYB119) from Populus trichocarpa that positively regulates anthocyanin production when expressed under the control of the CaMV 35S promoter in transgenic Arabidopsis. Amino acid sequence analysis revealed that PtrMYB119 is highly homologous to Arabidopsis PAP1 (PRODUCTION OF ANTHOCYANIN PIGMENT1), a well-known transcriptional activator of anthocyanin biosynthesis. Independently produced transgenic poplars overexpressing PtrMYB119 or PtrMYB120 (a paralogous gene to PtrMYB119) (i.e., 35S::PtrMYB119 and 35S::PtrMYB120, respectively) showed elevated accumulation of anthocyanins in the whole plants, including leaf, stem and even root tissues. Using a reverse-phase high-performance liquid chromatography, we confirmed that the majority of the accumulated anthocyanin in our transgenic poplar is cyanidin-3-O-glucoside. Gene expression analyses revealed that most of the genes involved in the anthocyanin biosynthetic pathway were highly upregulated in 35S::PtrMYB119 poplars compared with the nontransformed control poplar. Among these genes, expression of PtrCHS1 (Chalcone Synthase1) and PtrANS2 (Anthocyanin Synthase2), which catalyze the initial and last steps of anthocyanin biosynthesis, respectively, was upregulated by up to 350-fold. Subsequent transient activation assays confirmed that PtrMYB119 activated the transcription of both PtrCHS1 and PtrANS2. Interestingly, expression of MYB182, a repressor of both anthocyanin and proanthocyanidin (PA) biosynthesis, was largely suppressed in 35S::PtrMYB119 poplars, while expression of MYB134, an activator of PA biosynthesis, was not changed significantly. More interestingly, high-level accumulation of anthocyanins in 35S::PtrMYB119 poplars did not have an adverse effect on plant growth. Taken together, our results demonstrate that PtrMYB119 and PtrMYB120 function as transcriptional activators of anthocyanin accumulation in both Arabidopsis and poplar.</P>

Polymorphisms in the neurokinin-2 receptor gene are associated with angiotensin-converting enzyme inhibitor-induced cough

Kim, T.-B.,Oh, S.-Y.,Park, H.-K.,Jeon, S.-G.,Chang, Y.-S.,Lee, K.-Y.,Cho, Y. S.,Chae, I.-H.,Kim, Y.-K.,Cho, S.-H.,Moon, H.-B.,Min, K.-U.,Kim, Y.-Y. Blackwell Publishing Ltd 2009 Journal of clinical pharmacy and therapeutics Vol.34 No.4

<P>Summary</P><P>Background and objective: </P><P>Treatment with angiotensin-converting enzyme (ACE) inhibitors can induce chronic cough in many patients. Genetic variations in the neurokinin 2 receptor gene (NK2R) are significantly associated with cough sensitivity to capsaicin.</P><P>Methods: </P><P>This study assessed the relationship between genetic polymorphisms in the NK2R gene and chronic cough in 91 patients taking ACE inhibitors. Patients included in the study did not have chest abnormalities, postnasal drip, gastroesophageal reflux or a recent history of upper respiratory infection.</P><P>Results: </P><P>We detected two single nucleotide polymorphisms in the NK2R gene (i.e., Gly231Glu and Arg375His). The allelic frequencies at amino acid 231 were 36·3% for Gly/Gly, 49·5% for Gly/Glu and 14·3% for Glu/Glu. The allelic frequencies at amino acid 375 were 74·7% for Arg/Arg, 24·2% for Arg/His and 1·1% for His/His. The prevalence of chronic cough in patients with the amino acid 231 genotype was 33·3% in Gly/Gly homozygotes, 24·4% in Gly/Glu heterozygotes and 0% in Glu/Glu homozygotes. There was a statistically significant association between chronic cough and the Glu/Glu allele (<I>P</I> = 0·028) when the data were analyzed with a recessive model. In addition, there was a significant inverse linear association between the number of Glu231 alleles and ACE inhibitor-related cough (<I>P </I>=<I> </I>0·026). The prevalence of chronic cough in patients with the amino acid 375 genotype was 22·1% in Arg/Arg homozygotes, 31·8% in Arg/His heterozygotes and 0% in His/His homozygotes, although none of these association were statistically significant.</P><P>Conclusion: </P><P>Our findings indicate that the Gly231Glu polymorphism is associated with a lower prevalence of ACE inhibitor-related cough.</P>

치환된 알킬 사슬 혼합물의 자기조립 단분자막 구조지 STM 연구

손승배,이해성,전일철,한재량,Son S.B.,Lee H.,Jeon I.C.,Hahn J.R. 한국진공학회 2006 Applied Science and Convergence Technology Vol.15 No.2

p-iodo-phenyl octadecyl ether (I-POE)와 p-iodo-phenyl docosyl ether (I-PDE)의 분자의 흑연표면에서의 자기조립과 이 두 분자로 이루어진 혼합물의 자기조립을 주사 터널링 현미경을 이용하여 연구하였다. 각 분자 시스템은 흑연 표면에서 head-to-tail 배향의 안정된 단분자막으로 자기조립한다. 혼합물 시스템에서는 I-POE와 I-PDE 분자들은 표면에서 섞이지 않고, 고립된 단분자막 도메인을 형성한다. 특히 I-POE 분자는 흑연 표면에서 단분자막 구조를 우선적으로 형성하는데 이는 알킬 사슬 길이와 작용기를 가진 헤드 그룹의 효과에 기인한다. The molecular assembly of p-iodo-phenyl octadecyl ether (I-POE), p-iodo-phenyl docosyl ether (I-PDE) and a binary mixture of these two molecules on graphite has been studied using a scanning tunneling microscope. Each molecular system self-assembles on the graphite surface to form a stable monolayer with a head-to-tail configuration. For the binary system, the I-POE and I-PDE molecules do not mix on the surface, preferring instead to form isolated monolayer domains. Here, the I-POE molecules are preferentially adsorbed on the graphite surface, due to the effects of alkyl chain length and the functional group on the monolayer structure.

Ribosomal protein S6 is a selective mediator of TRAIL-apoptotic signaling

Jeon, Y-J,Kim, I K,Hong, S-H,Nan, H,Kim, H-J,Lee, H-J,Masuda, E S,Meyuhas, O,Oh, B-H,Jung, Y-K Nature Publishing Group 2008 Oncogene Vol.27 No.31

TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) is a potent inducer of apoptosis in tumor cells and holds a promise as a therapeutic agent against cancer. To elucidate the death signaling evoked by TRAIL, we performed a functional genetic screening and rescued TRAIL-resistant Jurkat clones harboring ribosomal protein S6 (rpS6) cDNA in anti-sense frame. Reduction of rpS6 expression in Jurkat and HeLa cells attenuated apoptosis induced by TRAIL, but not those by other cell death signals, including tumor necrosis factor-α and cycloheximide, etoposide, doxorubicin, tunicamycin and staurosporine. Death receptor (DR) 4, but not DR5, was downregulated in rpS6 knockdown cells. Conversely, the sensitivity to TRAIL was increased by the ectopic expression of wild-type rpS6 and further by phospho-defective rpS6 mutant (S6-SS235,6AA), but not by phospho-mimic rpS6 mutant (S6-SS235,6DD). Also, unphosphorylatable rpS6 knock-in mouse embryo fibroblasts (rpS6<SUP>P−/−</SUP> MEFs) were more sensitive to TRAIL than control MEFs. In addition, SKHep-1 tumor cells, which express less phospho-rpS6 and are more sensitive to TRAIL than other tumor cells, became effectively desensitized to TRAIL after rpS6 knockdown. These results suggest that rpS6, especially in its unphosphorylated form, is a selective mediator of TRAIL-induced apoptosis.Oncogene (2008) 27, 4344–4352; doi:10.1038/onc.2008.73; published online 24 March 2008

Assignment of the phosphoinositide-3-kinase, class 3 (PIK3C3) gene to porcine chromosome 6q22→q23 by somatic cell and radiation hybrid panel mapping

Kim, J.H.,Lee, Y.S.,Park, E.W.,Seo, B.Y.,Cho, I.C.,Lee, J.G.,Oh, S.J.,Lee, J.H.,Jeon, J.T. S. Karger AG 2004 CYTOGENETIC AND GEN0ME RESEARCH Vol.108 No.4

<P> </P><P>Copyright © 2005 S. Karger AG, Basel</P>

Chlorpyrifos-induced biomarkers in Japanese medaka (Oryzias latipes)

Jeon, H. J.,Lee, Y. H.,Mo, H. h.,Kim, M. J.,Al-Wabel, M. I.,Kim, Y.,Cho, K.,Kim, T. W.,Ok, Y. S.,Lee, S. E. Springer 2016 Environmental Science and Pollution Research Vol. No.

<P>Chlorpyrifos (CHL) is an organophosphate compound that is widely used as an insecticide. Due to its repeated use and high environmental residual property, CHL is frequently passed into aquatic environments by runoff. Consequently, there may be an adverse effect on aquatic vertebrate animals, including fish. Therefore, in this study, we assessed how CHL affected Japanese medaka (Oryzias latipes). The acute toxicity of CHL in adult fish after 96 h of exposure was determined to be 212.50, 266.79, and 412.28 mu g L-1 (LC25, LC50, and LC95, respectively). Acetylcholinesterase (AChE), glutathione S-transferase (GST), and carboxylesterase (CE) activities were obtained from the livers of dead or surviving fish, and the results showed 4.8-fold lower, 4.5-fold higher, and 18.6-fold lower activities for the AChE, GST, and CE, respectively, for 64-h exposure at a concentration of 400 mu g L-1 of CHL. In the embryo toxicity test, curved spines were observed in embryos that were exposed to CHL for 48 h in a concentration-dependent manner. With identification of biomarkers for CHL in the fish, two protein peaks, 5550.86 and 5639.79 m/z, were found to be upregulated. These two proteins can be used as protein biomarkers for CHL contamination in aquatic systems. A phosphatidyl choline with an m/z ratio of 556.32 dramatically decreased after CHL exposure in the fish; thus, it may be considered as a lipid biomarker for CHL. It is assumed as the first report to identify a phospholipid biomarker using a lipidomics approach in fish toxicology. Taken together, these results demonstrated the adverse effects of CHL on Japanese medaka and reveal several candidate biomarkers that can be used as diagnostic tools for determining CHL.</P>

Assignment of phosphatidylinositol glycan, class K (PIGK) gene to porcine chromosome 6q32 by somatic cell and radiation hybrid panel mapping

Park, E.W.,Kim, J.H.,Lim, H.T.,Seo, B.Y.,Cho, I.C.,Lee, J.G.,Oh, S.J.,Cheong, I.C.,Lee, J.H.,Jeon, J.T. S. Karger AG 2004 CYTOGENETIC AND GEN0ME RESEARCH Vol.108 No.4

<P> </P><P>Copyright © 2005 S. Karger AG, Basel</P>

20대 여성의 데이트폭력 피해 경험과 우울, 불안 및 신체화의 관계

김수민,김예진,김유정,남지현,박금빈,박이준,안혜민,오세연,유가현,유다현,전예지,김옥수,길민지 이화여자대학교간호학회 2020 이화간호학회지 Vol.- No.54

Purpose: The purpose of this study is to examine the degree of date violence damage, depression, anxiety, and somatization among women in their 20s who had experienced date violence, and to investigate the correlations b etween the subjects’ experience of date violence damage and depression, anxiety and somatization. Method: The subject of this study were collected from September 04 to October 13, 2019, by posting advertisements on the women’s university community site in Seoul and a representative women;s community site on the Internet. 149 subjects were collected in their 20s who had experienced date violence from their partners. SPSS statistics 22.0 statistical program was used for data analysis, and standard deviation, T-test, ANOVA, and Pearson’s correlation coefficients were performed. Result: The results showed that the degree of experience of date violence was significantly correlated with symptoms of depression, anxiety, and somatization. Conclusion: Since there was correlations b etween depression, anxiety, and somatization symptoms in date violence victims, it is necessary to apply nursing intervention related to depression and anxiety to prevent somatization symptoms caused by date violence.

Antihypertensive peptide purified from Styela clava flesh tissue stimulates glucose uptake through AMP-activated protein kinase (AMPK) activation in skeletal muscle cells

Ko, S. C.,Kim, J. I.,Park, S. J.,Jung, W. K.,Jeon, Y. J. Springer Science + Business Media 2016 European food research and technology Vol.242 No.2

<P>Previously, our group described an antihypertensive peptide (Ala-His-Ile-Ile-Ile, MW: 565.3 Da) with angiotensin I-converting enzyme inhibitory and vasodilatory effects that was purified from Styela clava flesh tissue. In the present study, we investigated the metabolic effects of the antihypertensive peptide in skeletal muscle cells. We found that the antihypertensive peptide stimulated glucose uptake in differentiated L6 rat myoblast cells in a dose-dependent manner. Inhibition of AMP-activated protein kinase (AMPK) by compound C significantly inhibited the antihypertensive peptide-stimulated glucose uptake. Western blotting analyses revealed that the antihypertensive peptide stimulated AMPK phosphorylation and this enhancement could be specifically inhibited by compound C. Furthermore, the current study demonstrates that translocation of glucose transporter-4 (GLUT4) to the plasma membrane was stimulated by the antihypertensive peptide. In summary, the findings from this study suggest that the antihypertensive peptide may have beneficial effects on the glucose metabolism in skeletal muscle cells via a mechanism involving AMPK and possible stimulation of the intrinsic activity of GLUT4 transporter.</P>

[동력전단계부문] 하이브리드 차량의 개념설계용 시뮬레이션 프로그램의 개발

전순일(S.I.Jeon),조성태(S.T.Cho),조한상(H.S.Cho),박영일(Y.I.Park),이장무(J.M.Lee) 한국자동차공학회 1999 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

Because of the oil scarcity and the greenhouse effect in environment. fuel economy and emissions have been received much attention by car-makers. In the above crisis. a hybrid electric car can be one of the resonable solutions<br/> In this paper. we developed the simulation program for laying out the hybrid vehicle. This program consists of the algorithms: to compose hybrid system such as. the power-capacity-matching and the battery-size-selection. to simulate acceleration performance. fuel economy and emissions. to decide PSR(power split ratio). to make shift map. to appraise break-even point of the hybrid vehicle in comparison with I.C. engined vehicle. To test the usefulness of this simulation program. we applied it to a lay-out of the parallel hybrid bus