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RISS 인기검색어
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Distribution of DArT Markers in a Genetic Linkage Map of Tomato
Truong, Hai Thi Hong,Graham, Elaine,Esch, Elisabeth,Wang, Jaw-Fen,Hanson, Peter Korean Society of Horticultural Science 2010 원예과학기술지 Vol.28 No.4
토마토풋마름병에 저항성인 $Solanum$ $lycopersicum$ H7996와 극도감수성인 $S.$ $pimpinellifolium$ WVa700 간의 교배를 통해 획득한 재조합순계계통 $F_9$ 세대의 188개체를 이용하여 유전자연관지도를 작성하였다. 유전자지도는 DarT 260종, AFLP 74종, RFLP 4종, SNP 1종 및 SSR 22종 등 총 361종의 마커로 구성되었다. 작성된 유전자지도는 총 13개의 연관군(LG)에 2042.7cM을 포함하였으며 마커간의 평균지도거리는 5.7cM이고 이중 DArT마커는 평균 7.9cM당 1개가 분포하였다. SSR 마커의 분포를 기초로 작성된 11개 연관군들은 토마토 염색체의5번과 12번을 제외한 10개 염색체에 해당하였다. DArT 마커는 다른 마커들처럼 토마토 유전체 상에 고르게 분포하였으며, 인접 마커와의 상호분석(${\leq}$ 0.5cM) 결과 클러스터링 빈도가 13.5%인 AFLP 마커보다 3배 정도 높은 38.8%의 빈도로 최고치를 나타내었다. 본 연구를 통해 토마토에서 최초로 DarT 마커를 이용한 유전자연관지도를 작성하였다. A genetic linkage map was constructed using 188 $F_9$ RILs derived from a cross between $Solanum$ $lycopersicum$ H7996 (resistant to bacterial wilt) and $S.$ $pimpinellifolium$ WVa700 (highly susceptible to bacterial wilt). The map consisted of 361 markers including 260 DArTs, 74 AFLPs, 4 RFLPs, 1 SNP, and 22 SSRs. The resulting linkage map was comprised of 13 linkage groups covering 2042.7 cM. The genetic linkage map had an average map distance between markers of 5.7 cM, with an average DArT marker density of 1/7.9 cM. Based on the distribution of anchor SSR markers, 11 linkage groups were assigned to 10 chromosomes of tomato except chromosomes 5 and 12. The DArT markers were distributed across the genome in a similar way as other markers and showed the highest frequency of clustering (38.8%) at ${\leq}$ 0.5 cM intervals between adjacent markers, which is 3 times higher than AFLPs (13.5%). The present study is the first utilization of DArT markers in tomato linkage map construction.
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Distribution of DArT Markers in a Genetic Linkage Map of Tomato
Hai Thi Hong Truong,Elaine Graham,Elisabeth Esch,Jaw-Fen Wang,Peter Hanson 한국원예학회 2010 원예과학기술지 Vol.28 No.4
토마토풋마름병에 저항성인 Solanum lycopersicum H7996와 극도감수성인 S. pimpinellifolium WVa700 간의 교배를 통해 획득한 재조합순계계통 F? 세대의 188개체를 이용하여 유전자연관지도를 작성하였다. 유전자지도는 DarT 260종, AFLP 74종, RFLP 4종, SNP 1종 및 SSR 22종 등 총 361종의 마커로 구성되었다. 작성된 유전자지도는 총 13개의 연관군(LG)에 2042.7cM을 포함하였으며 마커간의 평균지도거리는 5.7cM이고 이중 DArT마커는 평균 7.9cM당 1개가 분포하였다. SSR 마커의 분포를 기초로 작성된 11개 연관군들은 토마토 염색체의5번과 12번을 제외한 10개 염색체에 해당하였다. DArT 마커는 다른 마커들처럼 토마토 유전체 상에 고르게 분포하였으며, 인접 마커와의 상호분석(≤0.5cM) 결과 클러스터링 빈도가 13.5%인 AFLP 마커보다 3배 정도 높은 38.8%의 빈도로 최고치를 나타내었다. 본 연구를 통해 토마토에서 최초로 DarT 마커를 이용한 유전자연관지도를 작성하였다. A genetic linkage map was constructed using 188 F? RILs derived from a cross between Solanum lycopersicum H7996 (resistant to bacterial wilt) and S. pimpinellifolium WVa700 (highly susceptible to bacterial wilt). The map consisted of 361 markers including 260 DArTs, 74 AFLPs, 4 RFLPs, 1 SNP, and 22 SSRs. The resulting linkage map was comprised of 13 linkage groups covering 2042.7 cM. The genetic linkage map had an average map distance between markers of 5.7 cM, with an average DArT marker density of 1/7.9 cM. Based on the distribution of anchor SSR markers, 11 linkage groups were assigned to 10 chromosomes of tomato except chromosomes 5 and 12. The DArT markers were distributed across the genome in a similar way as other markers and showed the highest frequency of clustering (38.8%) at ≤ 0.5 cM intervals between adjacent markers, which is 3 times higher than AFLPs (13.5%). The present study is the first utilization of DArT markers in tomato linkage map construction.
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Recent Activities on Breeding Anthracnose Resistant Pepper at AVRDC- The World Vegetable Center
Myeong-Cheoul Cho,Patcharaporn Suwor,Shin-Wen Lin,Zong-Ming Sheu,Jaw-Fen Wang,Suchila Techawongstien,Roland Schafleitner,Paul Gniffke,Sanjeet Kumar 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Pre- and postharvest anthracnose fruit rot is a serious disease of hot peppers (Capsicum annuum) throughout the world. AVRDC has pursued breeding for resistance to anthracnose for more than 10 years and has distributed a number of resistant lines in Asia and Africa. Recently AVRDC has identified highly aggressive isolates of Colletotrichum acutatum that have prompted renewed efforts to identify new anthracnose resistance genes. This study aimed to characterize resistance to specific pathogen strains in an array of newly identified breeding lines, and to validate one simple sequence repeat (SSR) and two sequence characterized amplified region (SCAR) markers linked to the anthracnose resistance locus. Forty-four accessions and two populations (two resistant parents and one susceptible parent, to F1, four backcross populations, and two F2 populations) are currently (Spring 2012) being screened against two pathotypes of C. acutatum collected in Taiwan. Pepper entries include C. annuum, C. baccatum, C. chinense, and their inter-specific progenies. Screening methods include field screening, spray and microinjection assays on green and red-ripe fruits, and molecular assays using SSR and SCAR markers linked to anthracnose resistance. Progress will be shared on initial screening results, evaluation of horticultural characteristics, and selection of potential lines for crossing programs.
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Selection of Anthracnose Resistant Chili Pepper lines at AVRDC-The World Vegetable Center
Myeong-Cheoul Cho,So-Young Kim,Shih-Wen Lin,Patcharaporn Suwor,Shieh-Sheue Chin,Zong-Ming Sheu,Jaw-fen Wang,Jae-Bok Yoon,Byung-Soo Kim,Techawongstien Suchila,Sanjeet Kumar 한국원예학회 2013 한국원예학회 학술발표요지 Vol.2013 No.5
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