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Jatoi, Ghulam Hussain,Lihua, Guo,Xiufen, Yang,Gadhi, Muswar Ali,Keerio, Azhar Uddin,Abdulle, Yusuf Ali,Qiu, Dewen The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.3
Here, we reported a novel secreted protein elicitor PeBL2 from Brevibacillus laterosporus A60, which can induce hypersensitive response in tobacco (Nicotiana benthamiana). The ion-exchange chromatography, high-performance liquid chromatography (HPLC) and mass spectrometry were performed for identification of protein elicitor. The 471 bp PeBL2 gene produces a 17.22 kDa protein with 156 amino acids containing an 84-residue signal peptide. Consistent with endogenous protein, the recombinant protein expressed in Escherichia coli induced the typical hypersensitive response (HR) and necrosis in tobacco leaves. Additionally, PeBL2 also triggered early defensive response of generation of reactive oxygen species ($H_2O_2$ and $O_2{^-}$) and systemic resistance against of B. cinerea. Our findings shed new light on a novel strategy for biocontrol using B. laterosporus A60.
Ghulam Hussain Jatoi,Guo Lihua,Yang Xiufen,Muswar Ali Gadhi,Azhar Uddin Keerio,Yusuf Ali Abdulle,Dewen Qiu 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.3
Here, we reported a novel secreted protein elicitor PeBL2 from Brevibacillus laterosporus A60, which can induce hypersensitive response in tobacco (Nicotiana benthamiana). The ion-exchange chromatography, high-performance liquid chromatography (HPLC) and mass spectrometry were performed for identification of protein elicitor. The 471 bp PeBL2 gene produces a 17.22 kDa protein with 156 amino acids containing an 84-residue signal peptide. Consistent with endogenous protein, the recombinant protein expressed in Escherichia coli induced the typical hypersensitive response (HR) and necrosis in tobacco leaves. Additionally, PeBL2 also triggered early defensive response of generation of reactive oxygen species (H2O2 and O2 -) and systemic resistance against of B. cinerea. Our findings shed new light on a novel strategy for biocontrol using B. laterosporus A60.
Abro, Manzoor Ali,Sun, Xiang,Li, Xingchun,Jatoi, Ghulam Hussain,Guo, Liang-Dong The Korean Society of Plant Pathology 2019 Plant Pathology Journal Vol.35 No.6
Endophytic fungi have received much attention as plant growth promoters as well as biological control agents against many plant pathogens. In this study, 30 endophytic fungal species, isolated from various plants in China, were evaluated using in vitro dual culture assay against Fusarium oxysporum f. sp. cucumerinum, causing wilt in cucumber. The results of the present study clearly showed that all the 30 endophytic fungal isolates were highly capable of inhibiting the mycelial colony growth of Fusarium oxysporum f. sp. cucumerinum with inhibition % over 66% as compared to control treatments. Among all of them, 5 isolates were highly effective such as, Penicillium sp., Guignardia mangiferae, Hypocrea sp., Neurospora sp., Eupenicillium javanicum, and Lasiodiplodia theobromae, respectively. The Penicillium sp. and Hypocrea sp. were highly effective as compared to other isolates. From in vitro results 10 best isolates were selected for greenhouse studies. The results of the greenhouse studies showed that among all of them 3 endophytic fungal isolates successfully suppressed wilt severity when co-inoculation with pathogen Fusarium. oxysporum f. sp. cucumerinum. The endophytic fungi also enhanced plant growth parameters of the host plants, the antagonistic fungal isolates increased over all plant height, aerial fresh, and dry weight as compared to control.
Xin Xiong Chang,Nabisab Mujawar Mubarak,Shaukat Ali Mazari,Abdul Sattar Jatoi,Awais Ahmad,Mohammad Khalid,Rashmi Walvekar,E.C. Abdullah,Rama Rao Karri,M.T.H Siddiqui,Sabzoi Nizamuddin 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.104 No.-
The concept of green chemistry has attracted attention due to the green synthesis and ecofriendly natureof the compounds leading to the green and sustainable chemical industries and processes. Chitosan is anecofriendly material, which is biodegradable, non-toxic, and biocompatible. It has the potential to bemodified into biofilms for various applications such as biomedical, packaging, and pharmaceutical fields. Nevertheless, some poor properties of chitosan restrict its wide applications. The incorporation ofnanocellulose fillers into chitosan matrix can enhance the mechanical and thermal properties of chitosan. Cellulose nanomaterials can be achieved through chemical and mechanical modifications. The commontype of nanocellulose are cellulose nanofibers (CNFs), cellulose nano-whiskers (CNWs), tunicate CNCs (t-CNCs), algae cellulose particles (AC) and bacterial cellulose particles (BC). Nanocellulose are applied asthe reinforcement fillers in various polymer matrices such as polysaccharides, proteins, lipids, polylacticacid etc. Deep eutectic solvents (DES) are relatively novel green solvents, which can be applied in variousfields. DES are widely applied in metal processing, polymer processing and synthesis. Even though thereare not much studies available on DES for synthesis of nanocomposite films; however they are used aseco-friendly solvents in manufacturing processes. This study reviews the discovery, structure, propertiesof chitosan and cellulose, their derivatives and applications. In addition, the paper also discusses theproperties of DES and their applications.
Evaluation and future predictions of air pollutants level in Karachi city
Mukwana, Kishan Chand,Samo, Saleem Raza,Jakhrani, Abdul Qayoom,Tunio, Muhammad Mureed,Jatoi, Abdul Rehman Techno-Press 2017 Advances in environmental research Vol.6 No.2
The purpose of this study was to determine the present air pollutant concentrations and predicted levels for next 30 years in urban environment of Karachi city. For that, a total of fifty measurements were made for each twenty selected locations of the city. The locations were selected on the basis of land use pattern such as residential, commercial, industrial settlements, open areas, congested traffic and low traffic areas for investigation of air pollutants variability and intensity. The measurements were taken continuously for six months period using PM Meter, Model AEROCET 531 and Ambient Air Quality Meter, Model AAQ 7545. The concentration of air pollutants were found higher at Al Asif Square and Maripur Road due to higher intensity of traffic and at Korangi Crossing because of industrial areas. The level of pollutants was lower at Sea View owing to lower traffic congestion and transportation of pollutants by sea breezes.
Manzoor Ali Abro,Xiang Sun,Xingchun Li,Ghulam Hussain Jatoi,Liang-Dong Guo 한국식물병리학회 2019 Plant Pathology Journal Vol.35 No.6
Endophytic fungi have received much attention as plant growth promoters as well as biological control agents against many plant pathogens. In this study, 30 endophytic fungal species, isolated from various plants in China, were evaluated using in vitro dual culture assay against Fusarium oxysporum f. sp. cucumerinum, causing wilt in cucumber. The results of the present study clearly showed that all the 30 endophytic fungal isolates were highly capable of inhibiting the mycelial colony growth of Fusarium oxysporum f. sp. cucumerinum with inhibition % over 66% as compared to control treatments. Among all of them, 5 isolates were highly effective such as, Penicillium sp., Guignardia mangiferae, Hypocrea sp., Neurospora sp., Eupenicillium javanicum, and Lasiodiplodia theobromae, respectively. The Penicillium sp. and Hypocrea sp. were highly effective as compared to other isolates. From in vitro results 10 best isolates were selected for greenhouse studies. The results of the greenhouse studies showed that among all of them 3 endophytic fungal isolates successfully suppressed wilt severity when co-inoculation with pathogen Fusarium. oxysporum f. sp. cucumerinum. The endophytic fungi also enhanced plant growth parameters of the host plants, the antagonistic fungal isolates increased over all plant height, aerial fresh, and dry weight as compared to control.