Polymorphisms of <i>KCNJ11</i> (Kir6.2 gene) are associated with Type 2 diabetes and hypertension in the Korean population

Koo, B. K.,Cho, Y. M.,Park, B. L.,Cheong, H. S.,Shin, H. D.,Jang, H. C.,Kim, S. Y.,Lee, H. K.,Park, K. S. Blackwell Publishing Ltd 2007 Diabetic medicine Vol.24 No.2

<P>Abstract</P><P>Aims </P><P>Kir6.2 is found in the pancreatic B-cell, cardiac and skeletal muscle and non-vascular smooth muscle. <I>KCNJ11</I>, encoding Kir6.2, has been shown to be associated with both Type 2 diabetes mellitus and cardiovascular disease in several populations. In this study, we investigated whether polymorphisms in <I>KCNJ11</I> are associated with Type 2 diabetes and other metabolic phenotypes in the Korean population.</P><P><B>Methods </B></P><P>We sequenced <I>KCNJ11</I> to identify common polymorphisms using 24 Korean DNA samples. Of the 14 polymorphisms found in <I>KCNJ11</I>, six common ones [genomic sequence (g.)−1709A>T, g.−1525T>C, g.67G>A (E23K), g.570C>T (A190A), g.1009A>G (I337V), and g.1388C>T] were genotyped in 761 Type 2 diabetic patients and in 630 non-diabetic subjects.</P><P><B>Results</B> </P><P>All the polymorphic loci in <I>KCNJ11</I> are in strong linkage disequilibrium in the Korean population and act as one haplotype block. g.67G>A and g.1009A>G were associated with an increased risk of Type 2 diabetes [age, sex, and body mass index (BMI)-adjusted odds ratios (OR) = 1.376 (1.085–1.745), <I>P</I> = 0.008 and 1.411 (1.111–1.791), <I>P</I> = 0.005, respectively], as was one haplotype (A-T-A-C-G-C in the order of polymorphisms as shown above) containing g.67A and g.1009G [OR = 1.359 (1.080–1.709), <I>P</I> = 0.009]. The haplotype (A-T-A-C-G-C) was also strongly associated with hypertension [OR = 1.655 (1.288–2.126), <I>P</I> < 0.001].</P><P><B>Conclusions </B></P><P>Polymorphisms in <I>KCNJ11</I> are associated with Type 2 diabetes and also with hypertension in the Korean population.</P>

Efficacy of HVT-IBD vector vaccine compared to attenuated live vaccine using in-ovo vaccination against a Korean very virulent IBDV in commercial broiler chickens

Roh, J.-H.,Kang, M.,Wei, B.,Yoon, R.-H.,Seo, H.-S.,Bahng, J.-Y.,Kwon, J.-T.,Cha, S.-Y.,Jang, H.-K. Elsevier 2016 Poultry science Vol.95 No.5

<P>The production performance, efficacy, and safety of two types of vaccines for infectious bursal disease virus (IBDV) were compared with in-ovo vaccination of Cobb 500 broiler chickens for gross and microscopic examination of the bursa of Fabricius, bursa/body weight (b/B) ratio, flow cytometry, and serologic response to Newcastle disease virus (NDV) vaccination. One vaccine was a recombinant HVT-IBD vector vaccine (HVT as for herpesvirus of turkeys) and the other was an intermediate plus live IBDV vaccine. A significant difference was detected at 21 d. Eight of 10 chickens that received the IBDV live vaccine had severe bursal lesions and a relatively low b/B ratio of 0.95, and an inhibited NDV vaccine response. On the other hand, the HVT-IBD vector vaccine resulted in mild bursal lesions and a b/B ratio of 1.89. Therefore, the live vaccine had lower safety than that of the HVT-IBD vector vaccine. To determine the protective efficacy, chickens were intraocularly challenged at 24 d. Eight of 10 chickens in the IBDV live vaccination group showed gross and histological lesions characterized by hemorrhage, cyst formation, lymphocytic depletion, and a decreased b/B ratio. In contrast, the HVT-IBD vector vaccinated chickens showed mild gross and histological lesions in three of 10 chickens with a b/B ratio of 1.36, which was similar to that of the unchallenged controls. Vaccinated chickens showed a significant increase in IBDV antibody titers, regardless of the type of vaccine used. In addition, significantly better broiler flock performance was observed with the HVT-IBD vector vaccine compared to that of the live vaccine. Our results revealed that the HVT-IBD vector vaccine could be used as an alternative vaccine to increase efficacy, and to have an improved safety profile compared with the IBDV live vaccine using in-ovo vaccination against the Korean very virulent IBDV in commercial broiler chickens.</P>

18Ni 마르에이징강의 피로특성 및 유한요소해석

장경천,국중민,최병희,정재강,최병기 한국공작기계학회 2004 한국공작기계학회 춘계학술대회논문집 Vol.2004 No.-

Effects of Nb(Niobium) contents and solution annealing on the strength and fatigue life of 18%Ni maraging steel commonly using in aircraft, space field, nuclear energy, and vehicle etc. were investigated. Also the fatigue life stress intensity factor were compared experiment result and FEA(finite element analysis) result. The more Nb content, the higher or the lower fatigue life on base metal specimens or solution annealed specimens showing that the fatigue life was almost the same. The maximum stresses of X, Y, and Z axis direction showed about 2.12×10²MPa, 4.40×10²MPa and 1.32x10²MPa respectively. The Y direction stress showed the highest because of the same direction as the loading direction. The fatigue lives showed about 7% lower FEA result than experiment result showing almost invariable error every analyzed cycle. Stress intensity factor of the FEA result was lower about 3.5~10% than that of the experiment result showing that the longer fatigue crack length, the higher error. It considered that the cause for the difference was the modeled crack tip having always the same shape and condition regardless of the crack growth.

Conduritol F, the discriminant marker between C. wilfordii and C. auriculatum by ¹H NMR spectroscopy

Jang, H.S.,Jeong, B.,Choi, S.Y.,Jang, G.H.,Park, K.C.,Kwon, Y.S.,Yang, H. Academic Press 2017 Microchemical Journal Vol. No.

<P>Quantitative H-1 nuclear magnetic resonance (qNMR) spectroscopy is a powerful and versatile technique to enable the absolute quantification of specific components in a mixture with excellent reproducibility and robustness. In the present study, qNMR analysis was applied to conduritol F, a chemical marker with the potential to distinguish between C wilfordii and C auriculatum. We found that the signals of H-5 and H-6 of conduritol F were well-separated from others with purities sufficient to be used to distinguish between C wilfordii and C. auriculatum. This simple methodology can be applied to identify one or two species in products or powdered herbs widely distributed in the markets. (C) 2017 Elsevier B.V. All rights reserved.</P>

A Clinical and Histopathological Study of Experimental Canine Scabies

Hong, J. K Korean Dermatological Association 1998 Annals of Dermatology Vol.10 No.4

Lactoferrin causes IgA and IgG2b isotype switching through betaglycan binding and activation of canonical TGF-β signaling

Jang, Y-S,Seo, G-Y,Lee, J-M,Seo, H-Y,Han, H-J,Kim, S-J,Jin, B-R,Kim, H-J,Park, S-R,Rhee, K-J,Kim, W-S,Kim, P-H Society for Mucosal Immunology 2015 Mucosal immunology Vol.8 No.4

<P>Lactoferrin (LF), a pleiotropic iron-binding glycoprotein, is known to modulate the humoral immune response. However, its exact role in Ig synthesis has yet to be elucidated. In this study, we investigated the effect of LF on Ig production by mouse B cells and its underlying mechanisms. LF, like transforming growth factor (TGF)-beta 1, stimulated B cells to produce IgA and IgG2b, while downregulating other isotypes. Using limiting dilution analysis, LF was shown to increase the frequency of IgA-secreting B-cell clones. This was paralleled by an increase in Ig germ-line alpha (GL alpha) transcripts, indicating that LF plays a role as an IgA switch factor. Interestingly, LF directly interacted with betaglycan (TGF-beta receptor III, T beta RIII) and in turn induced phosphorylation of T beta RI and Smad3 through formation of the T beta RIII/T beta RII/T beta RI complex, leading to IgA isotype switching. Peroral administration of LF increased intestinal/serum IgA production as well as number of IgA plasma cells in lamina propria. Finally, we found that LF has an adjuvant activity when nontoxigenic Salmonella typhimurium was inoculated perorally, conferring protection against intragastrical infection of toxigenic S. typhimurium. These results suggest that LF has an important effect on the mucosal/systemic IgA response and can contribute to protection against intestinal pathogens.</P>

Fabrication of Magnesium Diboride Thin Films by Aerosol Deposition

Sinha, B.B.,Chung, K.C.,Jang, S.H.,Hahn, B.D.,Park, D.S.,Wang, X.L.,Dou, S.X. The Korean Superconductivity Society 2011 Progress in superconductivity Vol.13 No.2

Superconducting $MgB_2$ thin films were fabricated by using a novel aerosol deposition technique wherein the pre-reacted powder is directly transformed into respective thin film. The formed thin films were characterized by X-ray diffraction technique and FE-SEM to understand its structure and morphology and the superconducting behavior has been characterized with the four probe resistivity measurement. The as-deposited thin films were formed into the frustrated amorphous structure, which were relaxed on the further heat treatment at $900^{\circ}C$ for 3 hrs. The relaxed amorphous $MgB_2$ thin films showed a comparatively high superconducting onset at about 38 K.

Synthesis and luminescent features of NaCaPO₄:Tb³⁺ green phosphor for near UV-based LEDs

Ratnam, B.V.,Jayasimhadri, M.,Bhaskar Kumar, G.,Jang, K.,Kim, S.S.,Lee, Y.I.,Lim, J.M.,Shin, D.S.,Song, T.K. Elsevier Sequoia 2013 JOURNAL OF ALLOYS AND COMPOUNDS Vol.564 No.-

<P>An efficient green emitting Tb3+ doped NaCaPO4 (NCP) phosphor was synthesized by using conventional solid-state reaction for solid-state lighting applications. X-ray diffraction (XRD), field emission scanning electron microscope (FE-SEM), FT-IR, emission and excitation properties were extensively investigated for NCP phosphors. X-ray diffraction analysis confirmed the formation of NaCaPO4 with orthorhombic structure. The excitation spectrum consists of strong 4f-4f transition at around 370 nm, which has higher intensity than the f-d transition. Emission spectra indicated that this phosphor can be efficiently excited by UV light in the range from 250 to 400 nm, and shows strong emission band centered at 547 nm. Analysis of the emission spectra with different Tb3+ concentrations revealed that the optimum dopant concentration for these NCP phosphors is about 5 mol% of Tb3+. Diminishing of D-5(3) level and increasing of D-5(4) level emission intensity with the Tb3+ concentration explained successfully. The emission color was analyzed and confirmed with the help of chromaticity coordinates and color temperature. The excellent luminescent properties of NaCaPO4: Tb3+ phosphor makes it as a potential green phosphor upon near-UV LED excitation. (C) 2013 Elsevier B.V. All rights reserved.</P>

Collective effect of hydrogen in argon and Mg as ambiance for the heat treatment on MgB₂

Sinha, B.B.,Jang, S.H.,Chung, K.C. The Korea Institute of Applied Superconductivity a 2014 한국초전도저온공학회논문지 Vol.16 No.2

Magnesium diboride superconductor is still of considerable interest because of its appealing characteristics towards application mainly at around 20 K. Unlike Nb-based superconductors, $MgB_2$ can be operated by cryogen-free cooler which provides a cost effective alternative at low field of around 2-5 T. To explore this operating field region considerable efforts are necessary to marginally improve the superconducting properties of $MgB_2$. Under this situation, even the heat treatment environment during the synthesis is considered as an important factor. The addition of $H_2$ gas in small amount with Ar as a mixed gas during annealing has an adverse effect on the superconducting properties of $MgB_2$. It is although interesting to find that the presence of Mg vapor along with hydrogen during heat treatment results in the appreciable improvement in the flux pinning and the overall response of the critical current density for the ex-situ $MgB_2$ samples.

자유드로잉 제품과 패드를 설치한 드로잉 제품의 특성

고관영,명노훈,하종성,이창수,최창권,최병용,허기영,김광수,김동수,장종훈,장윤철,김세환,오정민,하민정,박향자 울산과학대학 2000 연구논문집 Vol.27 No.1

본 연구는 자체 제작한 전체 패드가 부착된 드로잉 금형을 이용하여 SUS, 연강, AI, 6:4황동 판재를 드로잉 가공하여 생산된 제품의 특성을 자유드로잉 하여 얻은 제품의 특성과 비교 분석하였다. SUS 판재의 경우 제품에 발생하는 현상은 벽 주름, 구변주름, 수직 파단, 드로잉상처, 이어링이 발생하였으며 길이변화는 펀치어깨부분에서 가장 많이 늘어남을 볼 수 있었고 벽 주름 부분에 있어서 접혀진 부분은 상대적으로 적게 늘어남을 볼 수 있었다. 연강 판재는 SUS 판재에서 보여준 현상을 나타내었으나 SUS 판재에 비해 수직 파단은 적게 일어난 반면 이어링이 더 크게 일어났다. AI 판재의 경우 수직 파단은 일어나지 않았으나 플랜지 주름과 밑면터짐이 발생하였다. 6:4황동의 경우는 전체패드의 AI 판재 드로잉에서와 같이 밑면터짐과 플랜지 주름의 발생이 일어났다.