Upregulation of duck interleukin-17A during Riemerella anatipestifer infection

Fernandez, C.P.,Kim, W.H.,Diaz, J.A.R.,Jeong, J.,Afrin, F.,Kim, S.,Jang, H.K.,Lee, B.H.,Yim, D.,Lillehoj, H.S.,Min, W. Pergamon Press ; Elsevier Science 2016 DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY Vol.63 No.-

<P>Although IL-17 cytokines play critical roles in host defense immunity, dysregulated expression of these cytokines is associated with inflammation and autoimmune diseases. Riemerella anatipestifer is the most important infectious bacterium in the duck industry. Interestingly, not all avian species are equally susceptible to R. anatipestifer infection. This paper reports the first description of mortality rate, bacterial burden, and expression profiles of immune-related genes between ducks and chickens infected with R. anatipestifer. Ducks exhibited increased susceptibility to R. anatipestifer infection compared to chickens, as determined by mortality rate and bacterial burden. Comparative expression analyses of immune-related genes in R. anatipestifer-infected tissues obtained from both species revealed that TLR3, TLR7, IL-2, IL-4, and IFN-gamma transcript levels were higher in chickens, whereas TLR4 and IL-17A transcript levels were higher in ducks. Marked increases in expression of IL-17A and IL-6, but not TGF-beta, were associated with Th17 cell differentiation in duck splenic lymphocytes, but not in chicken splenic lymphocytes, stimulated with R. anatipestifer. Moreover, upregulation of IL-1 beta, IL-6, and IL-17A mRNA expressions, but not TGF-beta, was confirmed in the liver and spleen of ducks infected with R. anatipestifer, indicating that IL-17A is strongly associated with Riemerella infection in ducks. (C) 2016 Elsevier Ltd. All rights reserved.</P>

Novel anti-apoptotic mechanism of A20 through targeting ASK1 to suppress TNF-induced JNK activation

Won, M,Park, K A,Byun, H S,Sohn, K-C,Kim, Y-R,Jeon, J,Hong, J H,Park, J,Seok, J H,Kim, J M,Yoon, W-H,Jang, I-S,Shen, H M,Liu, Z G,Hur, G M Macmillan Publishers Limited 2010 CELL DEATH AND DIFFERENTIATION Vol.17 No.12

The zinc-finger protein A20 has crucial physiological functions as a dual inhibitor of nuclear factor-κB (NF-κB) activation and apoptosis in tumor necrosis factor (TNF) receptor 1 signaling pathway. Although the molecular basis for the anti-NF-κB function of A20 has been well elucidated, the anti-apoptotic function of A20 is largely unknown. Here, we report a novel mechanism underlying the anti-apoptotic function of A20: A20 blocks TNF-induced apoptosis through suppression of c-jun N-terminal kinase (JNK) by targeting apoptosis signal-regulating kinase1 (ASK1). First, the ectopic expression of A20 drastically inhibits TNF-induced JNK activation and apoptosis in multiple cell types including those deficient of NF-κB activation. Unexpectedly, the blunting effect of A20 on TNF-induced JNK activation is not mediated by affecting the TNFR1 signaling complex formation. Instead, A20 interacts with ASK1, an important MAPKK kinase in the JNK signaling cascade. More importantly, overexpression of wild-type A20, but not of mutant A20 (ZnF4; C624A, C627A), promotes degradation of the ASK1 through the ubiquitin-proteasome system. Taken together, the results from this study reveal a novel anti-apoptotic mechanism of A20 in TNF signaling pathway: A20 binds to ASK1 and mediates ASK1 degradation, leading to suppression of JNK activation and eventually blockage of apoptosis.

NMR Solution Structure of HP0827 (O25501_HELPY) from Helicobacter pylori: Model of the Possible RNA-binding Site

Jang, S.-B.,Ma, C.,Lee, J.-Y.,Kim, J.-H.,Park, S. J.,Kwon, A.-R.,Lee, B.-J. Oxford University Press 2009 The Journal of biochemistry Vol.146 No.5

<P>The HP0827 protein is an 82-residue protein identified as a putative ss-DNA-binding protein 12RNP2 Precursor from Helicobacter pylori. Here, we have determined 3D structure of HP0827 using Nuclear Magnetic Resonance. It has a ferredoxin-like fold, beta1-alpha1-beta2-beta3-alpha2-beta4 (alpha; alpha-helix and beta; beta-sheet) and ribonucleoprotein (RNP) motifs which are thought to be important in RNA binding. By using structural homologues search and analyzing electrostatic potential of surface, we could compared HP0827 with other RNA-binding proteins (sex-lethal, T-cell restricted intracellular antigen-1, U1A) to predict RNA-binding sites of HP0827. We could predict that beta sheets of HP0827, especially beta1 and beta3, are primary region for RNA binding. Consequently, similar to other RNA-binding proteins, RNP motifs (Y5, F45, F47), positively charged and hydrophobic regions (K32, R37, K40, K41, K43, R70, R73) are proposed as a putative RNA-binding sites. In addition, differences in amino acids composition of RNP motifs, N, C-terminal residues, loop-region fold and the orientation of alpha1-helix with other RNA recognition motif proteins could give specific biological functions to HP0827. Finally, the study on natural RNA target is also important to completely understand the biological function of HP0827.</P>

Microwave spectrum, structure and dipole moment of 3-fluorophenylacetylene (3FPA)

Jang, H.,Ka, S.,Peebles, S.A.,Peebles, R.A.,Oh, J.J. Elsevier Scientific Pub. Co 2016 Journal of molecular structure Vol.1125 No.-

The 6-18 GHz rotational spectrum of 3-fluorophenylacetylene (3FPA) was measured by chirped-pulse Fourier-transform microwave (CP-FTMW) spectroscopy. Rotational constants and quartic centrifugal distortion constants based on a Watson-A reduction were determined with 89 transitions; A = 3383.73821 (33) MHz, B = 1180.97617 (16) MHz, C = 875.26172 (12) MHz, Δ<SUB>J</SUB> = 0.0382 (13) kHz, Δ<SUB>K</SUB> = 1.316 (21) kHz, δ<SUB>J</SUB> = 13.93 (56) Hz, and δ<SUB>K</SUB> = 180.3 (60) Hz. An additional 12-13 transitions for each of eight <SUP>13</SUP>C isotopic species and Stark effects to determine dipole moment components were observed by Balle-Flygare FTMW spectroscopy. Gas phase molecular structures of 3FPA were derived via the least-square fitting (r<SUB>0</SUB>) and substitution (r<SUB>s</SUB>) methods using the moments of inertia of the isotopic species. The ring geometry is discussed and compared with previous studies of structures of monosubstituted benzene and crystalline solid structures of 3FPA.

Relating Hyperspectral Image Bands and Vegetation Indices to Corn and Soybean Yield

Jang Gab-Sue,Sudduth Kenneth A.,Hong Suk-Young,Kitchen Newell R.,Palm Harlan L. The Korean Society of Remote Sensing 2006 大韓遠隔探査學會誌 Vol.22 No.3

Combinations of visible and near-infrared (NIR) bands in an image are widely used for estimating vegetation vigor and productivity. Using this approach to understand within-field grain crop variability could allow pre-harvest estimates of yield, and might enable mapping of yield variations without use of a combine yield monitor. The objective of this study was to estimate within-field variations in crop yield using vegetation indices derived from hyperspectral images. Hyperspectral images were acquired using an aerial sensor on multiple dates during the 2003 and 2004 cropping seasons for corn and soybean fields in central Missouri. Vegetation indices, including intensity normalized red (NR), intensity normalized green (NG), normalized difference vegetation index (NDVI), green NDVI (gNDVI), and soil-adjusted vegetation index (SAVI), were derived from the images using wavelengths from 440 nm to 850 nm, with bands selected using an iterative procedure. Accuracy of yield estimation models based on these vegetation indices was assessed by comparison with combine yield monitor data. In 2003, late-season NG provided the best estimation of both corn $(r^2\;=\;0.632)$ and soybean $(r^2\;=\;0.467)$ yields. Stepwise multiple linear regression using multiple hyperspectral bands was also used to estimate yield, and explained similar amounts of yield variation. Corn yield variability was better modeled than was soybean yield variability. Remote sensing was better able to estimate yields in the 2003 season when crop growth was limited by water availability, especially on drought-prone portions of the fields. In 2004, when timely rains during the growing season provided adequate moisture across entire fields and yield variability was less, remote sensing estimates of yield were much poorer $(r^2<0.3)$.

Fabrication of pulsed laser deposited Ge doped CZTSSe thin film based solar cells: Influence of selenization treatment

Lokhande, A.C.,Chalapathy, R.B.V.,Jang, J.S.,Babar, P.T.,Gang, M.G.,Lokhande, C.D.,Kim, Jin Hyeok North-Holland 2017 Solar Energy Materials and Solar Cells Vol. No.

<P><B>Abstract</B></P> <P>In the present work, Ge doped CZTGeS thin films are pulsed laser deposited followed by annealing treatment in selenium environment. The influence of selenization condition on the structural, morphological, optical and electrical properties of the absorber thin films are investigated. The thin films characterized using X-ray diffraction (XRD) and Raman spectroscopy techniques confirm the formation of Kesterite CZTGeSSe thin film compound with dominant A1 mode vibration. The morphological and optical studies of the thin films reveal the formation of compact and void free microstructure with optimal band gap in the range of 1–1.2eV. The impact of selenization temperature on the quality of thin films has been studied and thin film solar cells are fabricated with CZTGeSSe absorbers grown at various annealing temperatures from 525 to 575℃ to evaluate the performance of devices as a function of an annealing temperature. The elemental Ge and Sn losses from the absorber compound confirmed from X-ray fluorescence spectroscopy (XRF) depended on the annealing temperature and linearly increased with increasing temperature affecting the optical, compositional and microstructural properties of the thin films. Compositional non uniformity is one of the factors that limit the performance of solar cell device. PLD technique due to its advantage of achieving precise stoichiometry control combined with optimized selenization conditions can potentially address the issue. Compared to solar cell fabricated from absorber compound annealed at 525 and 575℃, the solar cell fabricated from the absorber annealed at 550℃ exhibited the best conversion efficiency of 3.82% with V<SUB>oc</SUB> 434mV, J<SUB>sc</SUB> 18.33mA/cm<SUP>2</SUP>, FF 47.0% and retained nearly 90% power conversion efficiency (PCE) stability after time period of 60 days.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Pulsed laser deposition of Ge doped CZTS thin films. </LI> <LI> Band gap tuning of CZTGeS thin films with selenization treatment. </LI> <LI> Effect of annealing conditions on structural, optical and morphological properties of CZTGeSSe thin films. </LI> <LI> Fabrication of CZTGeSSe thin film solar cells with efficiency over 3.82%. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Computational studies of hole/electron transport in positional isomers of linear oligo-thienoacenes: Evaluation of internal reorganization energies using density functional theory

Thomas, A.,Chitumalla, R.K.,Puyad, A.L.,Mohan, K.V.,Jang, J. Elsevier Scientific Pub. Co 2016 Computational & theoretical chemistry Vol.1089 No.-

<P>The paper computationally investigates and compares the internal reorganization energies associated with the intermolecular transport of a hole and electron in a series of positional isomers of fused planar acenodithiophenes (S(n)a-c) with those of linear acenes (LA(n)). In particular, it is observed that, amongst the positional isomers, the para isomers (S(n)c) have got ultra-small reorganization energies than the other two positional isomers. The non-bonding character of frontier molecular orbitals (FMO) of these para isomers is the main reason for the existence of this phenomenon. Secondly, the nonbonding character in the FMO of these molecules is mediated by their open shell singlet biradical nature in ground state, as revealed by the Spin-flip time dependent density functional theory analysis. These aforementioned findings and characteristics of S(n)c series of molecules, strongly recommend that these molecules if synthesized will show high charge carrier mobility. Also we have revealed that biradicaloid nature of a molecule is new interesting design factor to achieve low reorganization energies. (C) 2016 Elsevier B.V. All rights reserved.</P>

인슐린 치료후 발생한 인슐린 항체에 의한 저혈당증 2예

김경아,이관우,김미림,김여주,장재남,배성권,손병관 대한내분비학회 1996 Endocrinology and metabolism Vol.11 No.2

Hirata et al. first described the association of insulin antibodies with hypoglycemia attacks and decreased glucose tolerance in a patient who had not previously received insulin injections. Since that time there have been additional reports in the Japanese literature. Insulin antibodies are present in most of the patients who received insulin for a period of time, usually exceeding six weeks. There were several reports of hypoglycemia in patients with non-insulin-dependent diabetes mellitus who had developed insulin antibody after insulin administration and also in patients with insulin dependent diabetes mellitus. It is well known that either insulin antibody to the lower affinity site or insulin receptor antibody can be a cause of hypoglycemia. Recently, we experienced two cases of hypoglycemia in patient with insulin dependent diabetes mellitus and non- insulin- dependent diabetes mellitus who had developed insulin antibody after insulin administration. Hereby we present these cases with review of the literature(1 Kor Soc Endocrinol 11:240 ~246, 1996).

Relating Hyperspectral Image Bands and Vegetation Indices to Corn and Soybean Yield

Gab Sue Jang,Kenneth A. Sudduth,Suk Young Hong,Newell R. Kitchen,Harlan L. Palm 大韓遠隔探査學會 2006 大韓遠隔探査學會誌 Vol.22 No.3

Combinations of visible and near-infrared (NIR) bands in an image are widely used for estimating vegetation vigor and productivity. Using this approach to understand within-field grain crop variability could allow pre-harvest estimates of yield, and might enable mapping of yield variations without use of a combine yield monitor. The objective of this study was to estimate within-field variations in crop yield using vegetation indices derived from hyperspectral images. Hyperspectral images were acquired using an aerial sensor on multiple dates during the 2003 and 2004 cropping seasons for corn and soybean fields in central Missouri. Vegetation indices, including intensity normalized red (NR), intensity normalized green (NG), normalized difference vegetation index (NDVI), green NDVI (gNDVI), and soil-adjusted vegetation index (SAVI), were derived from the images using wavelengths from 440 nm to 850 nm, with bands selected using an iterative procedure. Accuracy of yield estimation models based on these vegetation indices was assessed by comparison with combine yield monitor data. In 2003, late-season NG provided the best estimation of both com (r2=0.632) and soybean (r2=0.467) yields. Stepwise multiple linear regression using multiple hyperspectral bands was also used to estimate yield, and explained similar amounts of yield variation. Corn yield variability was better modeled than was soybean yield variability. Remote sensing was better able to estimate yields in the 2003 season when crop growth was limited by water availability, especially on drought-prone portions of the fields. In 2004, when timely rains during the growing season provided adequate moisture across entire fields and yield variability was less, remote sensing estimates of yield were much poorer (r2<0.3).

미국산 및 한국산 닭고기 다리살 및 날개살의 품질 특성

이무하,민중석,김일석,이상옥,장애라,오동훈 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.2

Physicochemicah microbiological and sensory characteristics of chicken thighs and wings of foreign and domestic origin were investigated to provide the basic information of quality. In comparison of the proximate analysis of compositions among the same part of different origin, AT(American thigh meats) showed the highest content in the crude protein and ash. KBW(domestic wing meats produced at A company) showed the lowest moisture contents, and the highest protein contents (P $lt; 0.001). In color, KAT(domestic thigh meats purchased at Korean traditional market) showed higher a value than the others but the lowest pH value. AT and AW( American wing meats) had the highest TBA values and the lowest level of total aerobic bacteria. In the descriptive analysis for raw meat, KBT(domestic thigh meats produced at A company) was given the highest acceptability score(P $lt; 0.05). The cooked American thigh and wing meats obtained the highest score in off-flavor and the lowest score in acceptability.