Carbon Monoxide Attenuates Dextran Sulfate Sodium-Induced Colitis via Inhibition of GSK-3 <i><i>β</i></i> Signaling

Uddin, Md. Jamal,Jeong, Sun-oh,Zheng, Min,Chen, Yingqing,Cho, Gyeong Jae,Chung, Hun Taeg,Joe, Yeonsoo Hindawi Publishing Corporation 2013 Oxidative medicine and cellular longevity Vol.2013 No.-

<P>Endogenous carbon monoxide (CO) is produced by heme oxygenase-1 (HO)-1 which mediates the degradation of heme into CO, iron, and biliverdin. Also, CO ameliorates the human inflammatory bowel diseases and ulcerative colitis. However, the mechanism for the effect of CO on the inflammatory bowel disease has not yet been known. In this study, we showed that CO significantly increases survival percentage, body weight, colon length as well as histologic parameters in DSS-treated mice. In addition, CO inhalation significantly decreased DSS induced pro-inflammatory cytokines by inhibition of GSK-3<I><I>β</I></I> in mice model. To support the in vivo observation, TNF-<I><I>α</I></I>, iNOS and IL-10 after CO and LiCl treatment were measured in mesenteric lymph node cells (MLNs) and bone marrow-derived macrophages (BMMs) from DSS treated mice. In addition, we determined that CO potentially inhibited GSK-3<I><I>β</I></I> activation and decreased TNF-<I><I>α</I></I> and iNOS expression by inhibition of NF-<I><I>κ</I></I>B activation in LPS-stimulated U937 and MLN cells pretreated with CO. Together, our findings indicate that CO attenuates DSS-induced colitis via inhibition of GSK-3<I><I>β</I></I> signaling in vitro and in vivo. Importantly, this is the first report that investigated the molecular mechanisms mediated the novel effects of CO via inhibition GSK-3<I><I>β</I></I> in DSS-induced colitis model.</P>

Inhibitory Effects of <i>Chung Hun Wha Dam Tang</i> (CHWDT) on High-Fat Diet-Induced Obesity via AMP-Activated Protein Kinase Activation

Uddin, Md. Jamal,Joe, Yeonsoo,Zheng, Min,Kim, Sena,Lee, Hoyoung,Kwon, Tae-Oh,Chung, Hun Taeg Hindawi Publishing Corporation 2012 Evidence-based Complementary and Alternative Medic Vol.2012 No.-

<P>The <I>Chung Hun Wha Dam Tang</I> (CHWDT) herbal combination was reported to cease dizziness and phlegm. However, the effect of CHWDT in obesity has not yet been known mechanically. Therefore, we investigated whether this CHWDT could protect the cells from lipogenesis, gluconeogenesis, and inflammation in both in vivo and in vitro. CHWDT significantly decreased body weight, epididymal and perirenal fat content without affecting feed intake in high-fat diet-induced obese mice model. Additionally, CHWDT inhibited obesity-induced SREBP1, FAS, PGC1<I><I>α</I></I>, G6Pase, PEPCK and increased CPT1, ACO, and LCAD genes expression in vivo and in vitro. Proinflammatory cytokines like TNF-<I><I>α</I></I> and iNOS expression were reduced by CHWDT in both Raw264.7 macrophages and HepG2 cells. In addition, NO production was also significantly decreased by CHWDT in LPS-stimulated macrophages. Furthermore, AMPK<I><I>α</I></I> activation by CHWDT was involved in inhibition of obesity by reducing triglycerides production and increasing CPT1 expression. Based on all of the results, we suggest that CHWDT has inhibitory effects on obesity-induced lipogenesis, gluconeogenesis, and inflammation via AMPK<I><I>α</I></I> activation.</P>

Carbon monoxide releasing molecule-2 protects mice against acute kidney injury through inhibition of ER stress

Uddin, Md Jamal,Pak, Eun Seon,Ha, Hunjoo The Korean Society of Pharmacology 2018 The Korean Journal of Physiology & Pharmacology Vol.22 No.5

Acute kidney injury (AKI), which is defined as a rapid decline of renal function, becomes common and recently recognized to be closely intertwined with chronic kidney diseases. Current treatment for AKI is largely supportive, and endoplasmic reticulum (ER) stress has emerged as a novel mediator of AKI. Since carbon monoxide attenuates ER stress, the objective of the present study aimed to determine the protective effect of carbon monoxide releasing molecule-2 (CORM2) on AKI associated with ER stress. Kidney injury was induced after LPS (15 mg/kg) treatment at 12 to 24 h in C57BL/6J mice. Pretreatment of CORM2 (30 mg/kg) effectively prevented LPS-induced oxidative stress and inflammation during AKI in mice. CORM2 treatment also effectively inhibited LPS-induced ER stress in AKI mice. In order to confirm effect of CO on the pathophysiological role of tubular epithelial cells in AKI, we used mProx24 cells. Pretreatment of CORM2 attenuated LPS-induced ER stress, oxidative stress, and inflammation in mProx24 cells. These data suggest that CO therapy may prevent ER stress-mediated AKI.

Room temperature ferroelectric effect and enhanced dielectric permittivity in Rochelle salt/PVA percolative composite films

Jamal Uddin,T.R. Middya,B.K. Chaudhuri 한국물리학회 2013 Current Applied Physics Vol.13 No.3

Rochelle salt (RS) filled polyvinyl alcohol (PVA) composite films have been prepared via a simple solution casting technique. The transport, dielectric and ferroelectric properties of the samples have been studied. The dielectric permittivity decreases slowly with increasing frequency and rise gradually with increasing temperature and RS contents in the composites. As the volume fraction of the RS reaches to percolation threshold (fc w0.0538), an abrupt increase in the dielectric permittivity (w403 almost 80 times higher compared to pure PVA with low loss w0.18 at 1 kHz and room temperature) occurs in the RS/PVA composite film, which is attributed to the formation of the conductive network in the matrix. Ferroelectric loops up to room temperature (300 K) and the slight increase in Curie temperature from 297 to 300 K have also been observed for percolative composite film. The developed composite material with low loss high dielectric permittivity and room temperature ferroelectric behaviors might be applied in the technological fields.

Screening of some Bangladeshi medicinal plants for in vitro antibacterial activity

Uddin, Shaikh Jamal,Rouf, Razina,Shilpi, Jamil Ahmed,Alamgir, Mohammad,Nahar, Lutfun,Sarker, Satyajit Dey Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.3

A total of 33 extracts representing 26 plant species belonging to 24 families were collected from different regions of Bangladesh, and screened for their in vitro antibacterial activity against several pathogenic Gram-positive and Gram-negative bacterial strains using the conventional disc diffusion method. The most potent activity was exhibited by the extracts of Aegiceras corniculatum, Alocasia fornicata, Ceriops decandra, Cuscuta reflexa, Lasia spinosa, Lantana camara, Pandanus foetidus and Xylocarpus granatum. The extracts of Abtilon indicum, Derris trifoliata, Dendrophthoe falcat, Ruellia tuberosa and X. moluccensis did not show any antibacterial properties at test concentrations.

A functional link between heme oxygenase-1 and tristetraprolin in the anti-inflammatory effects of nicotine

Jamal Uddin, Md.,Joe, Yeonsoo,Zheng, Min,Blackshear, Perry J.,Ryter, Stefan W.,Park, Jeong Woo,Chung, Hun Taeg Elsevier 2013 FREE RADICAL BIOLOGY AND MEDICINE Vol.65 No.-

<P><B>Abstract</B></P> <P>Nicotine stimulates the cholinergic anti-inflammatory pathway and prevents excessive inflammation by inhibiting the release of inflammatory cytokines from macrophages. We have previously reported that heme oxygenase-1 (HO-1) and tristetraprolin (TTP) are induced by nicotine and mediate the anti-inflammatory function of nicotine in macrophages. However, it was not clear whether the two molecules are functionally linked. In this study, we sought to determine whether HO-1 associates with TTP to mediate the anti-inflammatory effects of nicotine. Inhibition of HO-1 activity or HO-1 expression attenuated the effects of nicotine on STAT3 activation, TTP induction, and TNF-α production in LPS-treated macrophages. Induction of HO-1 expression increased the level of TTP in the absence of nicotine. In an LPS-induced endotoxemia model, HO-1 deficiency blocked the effects of nicotine on the STAT3 phosphorylation, TTP induction, and LPS-induced TNF-α production in the liver. Downregulation of STAT3 by siRNA attenuated the effect of nicotine on TTP expression and TNF-α production but did not affect the nicotine-mediated induction of HO-1. In TTP knockout mice, nicotine treatment enhanced HO-1 expression and STAT3 activation but failed to inhibit LPS-induced TNF-α production. Our results suggest that HO-1 and TTP are functionally linked in mediating the anti-inflammatory effects of nicotine; HO-1 is necessary for the induction of TTP by nicotine. This novel nicotine–HO-1–TTP signaling pathway provides new possibilities for the treatment of inflammatory diseases.</P>

IRG1 induced by heme oxygenase-1/carbon monoxide inhibits LPS-mediated sepsis and pro-inflammatory cytokine production

Jamal Uddin, M.,Joe, Y.,Kim, S. K.,Oh Jeong, S.,Ryter, S. W.,Pae, H. O.,Chung, H. T. Nature 2016 CELLULAR AND MOLECULAR IMMUNOLOGY Vol.13 No.2

<P>The immunoresponsive gene 1 (IRG1) protein has crucial functions in embryonic implantation and neurodegeneration. IRG1 promotes endotoxin tolerance by increasing A20 expression in macrophages through reactive oxygen species (ROS). The cytoprotective protein heme oxygenase-1 (HO-1), which generates endogenous carbon monoxide (CO), is expressed in the lung during Lipopolysaccharide (LPS) tolerance and cross tolerance. However, the detailed molecular mechanisms and functional links between IRG1 and HO-1 in the innate immune system remain unknown. In the present study, we found that the CO releasing molecule-2 (CORM-2) and chemical inducers of HO-1 increased IRG1 expression in a time-and dose-dependent fashion in RAW264.7 cells. Furthermore, inhibition of HO-1 activity by zinc protoporphyrin IX (ZnPP) and HO-1 siRNA significantly reduced expression of IRG1 under these conditions. In addition, treatment with CO and HO-1 induction significantly increased A20 expression, which was reversed by ZnPP and HO-1 siRNA. LPS-stimulated TNF-alpha was significantly decreased, whereas IRG1 and A20 were increased by CORM-2 application and HO-1 induction, which in turn were abrogated by ZnPP. Interestingly, siRNA against IRG1 and A20 reversed the effects of CO and HO-1 on LPS-stimulated TNF-alpha production. Additionally, CO and HO-1 inducers significantly increased IRG1 and A20 expression and downregulated TNF-alpha production in a LPS-stimulated sepsis mice model. Furthermore, the effects of CO and HO-1 on TNF-alpha production were significantly reversed when ZnPP was administered. In conclusion, CO and HO-1 induction regulates IRG1 and A20 expression, leading to inhibition of inflammation in vitro and in an in vivo mice model.</P>

Free radical scavenging activity of some Bangladeshi plant extracts

Uddin, Shaikh Jamal,Shilpi, Jamil Ahmad,Delazar, Abbas,Nahar, Lutfun,Sarker, Satyajit Dey Kyung Hee Oriental Medicine Research Center 2004 Oriental pharmacy and experimental medicine Vol.4 No.3

A number of plants from different geographical origins have been shown to possess antioxidant activity. Some of them have been developed as natural antioxidant formulations for food, cosmetic and other applications. Bangladeshi flora is a rich source of a range of plant species, many of which are medicinal plants, and have been used in the preparations of the Unani and Ayurvedic traditional medicines. There are no, or just a few, reports on any systematic screening of the extracts of Bangladeshi plants for free radical scavenging activity using DPPH assay available to date. As part of our on-going search for biological activity in Bangladeshi plants, Kadam (Anthocephalus chinensis), Goran (Ceriaps decandra), Swarnalata (Cuscuta reflexa), Gab (Diospyros peregrina), Sundari (Heritiera fomes), Dhundul (Xylocarpus granatum) and Possur (Xylocarpus mekongensis) have been selected for the assessment of their free radical scavenging activity, and studies on the contents of alkaloids, anthraqunones, flavonoids and tannins in these extracts. Most of these species have been used in traditional medicine in Bangladesh and other countries for the treatment of various illnesses ranging from common cold to cancer. All extracts, except the methanol extract of Cuscuta reflexa, displayed significant free radical scavenging activity in the DPPH assay $(RC_{50}$ values within the range of $2.75\;{\times}\;10^{-2}\;to\;4.7\;{\times}\;10^{-3}\;mg/mL)$. Among these extracts, the methanol extract of Xylocarpus granatum exhibited the most potent activity $(4.7\;{\times}\;10^{-3}\;mg/mL)$ and that of Cuscuta reflexa had the least activity $(1.64\;{\times}\;10^{-1}\;mg/mL)$. While none of these plants showed positive tests with Dragendorff's reagent, presence of low to moderate amounts of phenolic compounds, e.g. anthraquinones, flavonoids and tannins was evident in all of these plants, except for the methanolic extracts of C. reflexa and the barks of D. peregrina, which did not display any evidence for the presence of flavonoids and anthraquinones, respectively.

Screening of some Bangladeshi medicinal plants for in vitro antibacterial activity

Shaikh Jamal Uddin,Razina Rouf,Jamil Ahmed Shilpi,Mohammad Alamgir,Lutfun Nahar,Satyajit Dey Sarker 경희대학교 융합한의과학연구소 2008 Oriental Pharmacy and Experimental Medicine Vol.8 No.3

A total of 33 extracts representing 26 plant species belonging to 24 families were collected from different regions of Bangladesh, and screened for their in vitro antibacterial activity against several pathogenic Gram-positive and Gram-negative bacterial strains using the conventional disc diffusion method. The most potent activity was exhibited by the extracts of Aegiceras corniculatum, Alocasia fornicata, Ceriops decandra, Cuscuta reflexa, Lasia spinosa, Lantana camara, Pandanus foetidus and Xylocarpus granatum. The extracts of Abtilon indicum, Derris trifoliata, Dendrophthoe falcat, Ruellia tuberosa and X. moluccensis did not show any antibacterial properties at test concentrations. A total of 33 extracts representing 26 plant species belonging to 24 families were collected from different regions of Bangladesh, and screened for their in vitro antibacterial activity against several pathogenic Gram-positive and Gram-negative bacterial strains using the conventional disc diffusion method. The most potent activity was exhibited by the extracts of Aegiceras corniculatum, Alocasia fornicata, Ceriops decandra, Cuscuta reflexa, Lasia spinosa, Lantana camara, Pandanus foetidus and Xylocarpus granatum. The extracts of Abtilon indicum, Derris trifoliata, Dendrophthoe falcat, Ruellia tuberosa and X. moluccensis did not show any antibacterial properties at test concentrations.

Carbon monoxide releasing molecule-2 protects mice against acute kidney injury through inhibition of ER stress

Md Jamal Uddin,Eun Seon Pak,Hunjoo Ha 대한생리학회-대한약리학회 2018 The Korean Journal of Physiology & Pharmacology Vol.22 No.5

Acute kidney injury (AKI), which is defined as a rapid decline of renal function, becomes common and recently recognized to be closely intertwined with chronic kidney diseases. Current treatment for AKI is largely supportive, and endoplasmic reticulum (ER) stress has emerged as a novel mediator of AKI. Since carbon monoxide attenuates ER stress, the objective of the present study aimed to determine the protective effect of carbon monoxide releasing molecule-2 (CORM2) on AKI associated with ER stress. Kidney injury was induced after LPS (15 mg/kg) treatment at 12 to 24 h in C57BL/6J mice. Pretreatment of CORM2 (30 mg/kg) effectively prevented LPS-induced oxidative stress and inflammation during AKI in mice. CORM2 treatment also effectively inhibited LPS-induced ER stress in AKI mice. In order to confirm effect of CO on the pathophysiological role of tubular epithelial cells in AKI, we used mProx24 cells. Pretreatment of CORM2 attenuated LPS-induced ER stress, oxidative stress, and inflammation in mProx24 cells. These data suggest that CO therapy may prevent ER stress-mediated AKI.