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Sim Jae-Ryeong,Lee Jong-Yeol,Kim Sewon 한국응용생명화학회 2023 Applied Biological Chemistry (Appl Biol Chem) Vol.66 No.-
In allergic individuals, ingestion of wheat can lead to wheat-dependent exercise-induced anaphylaxis (WDEIA). Many studies have been conducted to find WDEIA allergen–deficient wheat, including by generating omega-5 gliadin antibodies. However, the reported antibodies have not been specific enough to detect omega-5 gliadins encoded on the 1B chromosome. In this study, we generated monoclonal antibodies against the major allergens causing WDEIA, omega-5 gliadins. Using these antibodies (mono-O5B-1C10), we assessed accumulation of omega-5 gliadins in wild-type and nullisomic-tetrasomic (NT) lines of the wheat (Triticum aestivum) varieties Chinese Spring (CS) by one- and two-dimensional gel electrophoresis, followed by Coomassie blue staining or immunoblotting with mono-O5B-1C10. We also tested mono-O5B-1C10 for major omega-5 gliadins in various wheat germplasms. Our results thus demonstrate the specificity of mono-O5B-1C10 for major omega-5 gliadins and potentially useful for identifying of omega-5 gliadin–deficient wheat varieties that should not cause WDEIA.
Effect of D-glucose feeding on mortality induced by sepsis
Kim, Sung-Su,Sim, Yun-Beom,Park, Soo-Hyun,Lee, Jae-Ryeong,Sharma, Naveen,Suh, Hong-Won The Korean Society of Pharmacology 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.1
Sepsis is the life-threatening response to infection which can lead to tissue damage, organ failure, and death. In the current study, the effect of orally administered D-glucose on the mortality and the blood glucose level induced by D-Galactosamine (GaLN)/lipopolysaccharide (LPS)-induced sepsis was examined in ICR mice. After various amounts of D-glucose (from 1 to 8 g/kg) were orally fed, sepsis was induced by injecting intraperitoneally (i.p.) the mixture of GaLN /LPS. Oral pre-treatment with D-glucose dose-dependently increased the blood glucose level and caused a reduction of sepsis-induced mortality. The oral post-treatment with D-glucose (8 g/kg) up to 3 h caused an elevation of the blood glucose level and protected the mortality observed in sepsis model. However, D-glucose post-treated at 6, 9, or 12 h after sepsis induction did not affect the mortality and the blood glucose level induced by sepsis. Furthermore, the intrathecal (i.t.) pretreatment once with pertussis toxin (PTX; $0.1{\mu}g/5ml$) for 6 days caused a reduction of D-glucose-induced protection of mortality and hyperglycemia. Furthermore, once the hypoglycemic state is continued up to 6 h after sepsis initiated, sepsis-induced mortality could not be reversed by D-glucose fed orally. Based on these findings, it is assumed that the hypoglycemic duration between 3 and 6 h after the sepsis induction may be a critical time of period for the survival. D-glucose-induced protective effect against sepsis-induced mortality appears to be mediated via activating PTX-sensitive G-proteins in the spinal cord. Finally, the production of hyperglycemic state may be critical for the survival against the sepsis-induced mortality.
박은지(Eun Ji Park),심재령(Jae-Ryeong Sim),양유정(Yu-Jeong Yang),이샛별(Saet Buyl Lee),김범기(Beom-Gi Kim),김세원(Sewon Kim),이종열(Jong-Yeol Lee) 한국육종학회 2022 한국육종학회지 Vol.54 No.4
Wheat (Triticum aestivum L.) is one of the world's three staple crops and accounts for approximately 20% of the total calories consumed by the world's population. It is known that wheat is a difficult crop to introduce foreign genes into, having a large genome (16 Gb) containing three highly related subgenomes (AABBDD). Owing to the low transformation efficiency of wheat, it is difficult to apply new technologies such as genome editing and basic research based on molecular biology, such as the discovery of useful genes and functional analysis. Recently, the completion of a wheat genome map by the International Wheat Genome Sequencing Consortium (IWGSC) and the development of a stable and reproducible wheat transformation system have accelerated research regarding the expression and control of useful genes. In this review, we introduce in detail the recently developed highly efficient Agrobacterium-mediated wheat transformation system and its applications in plant biotechnology, such as RNA interference (RNAi), overexpression, and gene editing using this system.