Development of a bivalent live vaccine against duck hepatitis A virus types 1 & 3 and vaccine program for breeder ducks in Korea

Jae?Hee Roh(노재희),Min Kang,Bai Wei,Se-Yeoun Cha,Jeong-Gon Cho,Hyung-Kwan Jang 한국가금학회 2017 한국가금학회 정기총회 및 학술발표회 Vol.2017 No.11

어린 오리에 치명적인 질병을 일으키는 오리바이러스성간염의 예방을 위해 혼합생백신 개발, 백신의 방어기전 연구 및 종오리백신 프로그램의 개발로 질병방어 및 제어를 목표로 연구를 진행하였다. 첫 번째, 국내에는 1형과 3형이 동시 유행하고 있으며, 최근에는 3형의 발생보고가 증가하는 추세이다. 특히 1형과 3형의 복합감염사례도 빈번히 발생하고 있다. 이런 상황에서 효율적인 질병관리를 위해 국내에 발생하는 오리간염 1형과 3형을 동시에 방어하는 혼합백신을 개발하고 그 효능을 확인하였으며, 운반 및 보관 용이성 향상을 위한 연구를 진행하였다. 두 번째, 오리감염 생백신의 3-5일 만에 조기면역을 획득하는 특성분석을 위해 세포성 및 체액성 면역반응을 평가하였다. Cytokine이 백신접종 그룹에서 증가하는 것을 확인하였고, 특히 IFN-α에서 강한 증가를 보였다. 또한 항체가 평가에서는 기존에 알려진 바와는 다르게 백신 후 3일째에 항체를 획득하였고, 10일째에는 최고수준의 항체가 수준을 나타냈다. 세 번째, 1주령이하의 어린 일령에 치명적인 질병의 특성상 모체이행항체에 의한 예방이 가장 효과적이라는 점에 착안하여 종오리백신 프로그램을 개발하고 농장에 적용하여 평가하였다. 종오리 농장 4개동 4천수의 종오리를 대상으로 백신을 실시하였으며, 모체의중화항체가(VN titer) 8 log2이상에서 후대병아리 14일령까지 100% 방어효능을 확인하였다. 모체이행항체의 전달률은 11%수준으로 나타났으며, 후대병아리의 항체가를 개체별 추적하여 계산한 결과 모체이행항체 반감기는 3-5일령임을 확인하였다. 본 연구에서는 오리에 치명적인 질병을 일으키는 오리바이러스성간염 예방을 위한 혼합생백신 개발에 관한 연구로 기존의 국내 상용화백신의 한계점을 극복하고, 국내에 유행하는 혈청형들을 단회백신으로 효과적으로 방어할 수 있는 혼합생백신을 개발하였으며, 백신의 특성상 조기면역에 대한 세포성 및 체액성 면역기전을 평가하였다. 또한 종오리백신 프로그램을 국내에 적용하여 모체이행항체에 의한 후대 병아리의 우수한 방어 효능을 확인하였다.

상업스포츠센터 재 소비 의향 및 구전커뮤니케이션 효과

노형철,송채훈,박형대,노재성 朝鮮大學校 스포츠科學硏究所 2003 스포츠科學硏究 Vol.14 No.-

All estimation about agineungjeok image and symbolic Imijia which is of commerce sports center image Hawibyeon is anateuma level, and intention about a ashes consumption and all appeared by anateuma level. And in service quality assessment connected with program withering member bicarbonate of soda and contrast for member affirmative .Therefore, service quality, service value, estimation level about image and ashes consumption intention that commerce sports center consumer is recognizing, word of mouth communication intention is contradictory more or less and also, bicarbonate of soda member in service quality assessment which service quality estimation connected with main equipment and employee is affirmative, and is involved with subsidiary facilities than another thing education level group bicarbonate of soda and withering member contrast for member affirmative . And estimation about symbolic image is affirmative, ashes consumption intention level is high. Exercise participation degree and service quality Hawibyeonin that other commerce sports center consumption experience Hawibyeon is do not influence in ashes consumption intention and word of mouth communication factor.

A Close Relationship between Non-Alcoholic Fatty Liver Disease Marker and New-Onset Hypertension in Healthy Korean Adults

Jae-Hyung Roh,Jae-Hyeong Park,Hanbyul Lee,Yong-Hoon Yoon,Minsu Kim,Yong-Giun Kim,Gyung-Min Park,Jae-Hwan Lee,In-Whan Seong 대한심장학회 2020 Korean Circulation Journal Vol.50 No.8

Background and Objectives: Nonalcoholic fatty liver disease (NAFLD) is an excessive accumulation of fat into the liver as a result of increased inflammation and insulin resistance. Although there can be common pathogenic mechanisms for NAFLD and hypertension associated with the development of cardiovascular diseases, little data are showing the association between NAFLD and hypertension in a large-scale cohort study. Thus, we evaluated the ability of the fatty liver index (FLI), a surrogate marker of NAFLD, to predict the development of hypertension in healthy individuals. Methods: We included 334,280 healthy individuals without known comorbidities who underwent the National Health check-ups in South Korea from 2009 to 2014. The association between the FLI and hypertension was analyzed using multivariate Cox proportional-hazards models. Results: During a median of 5.2 years' follow-up, 24,678 subjects (7.4%) had new-onset hypertension. We categorized total subjects into quartile groups according to FLI (range: Q1, 0–4.9; Q2, 5.0–12.5; Q3, 12.6–31.0; and Q4, >31.0). The incidence of hypertension was higher in subjects with the highest FLI than in those with the lowest FLI (Q4, 9,968 [11.9%] vs. Q1, 2,277 [2.7%]; p<0.001). There was a significant correlation between the highest FLI and an increased risk of new-onset hypertension (adjusted hazard ratio between Q4 and Q1, 2.330; 95% confidence interval, 2.218–2.448; p<0.001). FLI was significantly associated with an increased risk of new-onset hypertension regardless of baseline characteristics. Conclusions: Higher FLI was independently associated with increased risk of hypertension in a healthy Korean population.

Distal Radial Approach through the Anatomical Snuff Box for Coronary Angiography and Percutaneous Coronary Intervention

Jae-Hyung Roh,Jae-Hwan Lee 대한심장학회 2018 Korean Circulation Journal Vol.48 No.12

Role of Carotid Artery Stenting in Prevention of Stroke for Asymptomatic Carotid Stenosis: Bayesian Cross-Design and Network Meta-Analyses

Jae-Hyung Roh,Hyun Jun Cho,Jae-Hwan Lee,Yongku Kim,Yeongwoo Park,Jae-Hyeong Park,Hee-Soon Park,Minsu Kim,Hyang Gon Jin,Yeji Cheon,In-Whan Seong 대한심장학회 2020 Korean Circulation Journal Vol.50 No.4

Background and Objectives: There is insufficient evidence regarding the optimal treatment for asymptomatic carotid stenosis. Methods: Bayesian cross-design and network meta-analyses were performed to compare the safety and efficacy among carotid artery stenting (CAS), carotid endarterectomy (CEA), and medical treatment (MT). We identified 18 studies (4 randomized controlled trials [RCTs] and 14 nonrandomized, comparative studies [NRCSs]) comparing CAS with CEA, and 4 RCTs comparing CEA with MT from MEDLINE, Cochrane Library, and Embase databases. Results: The risk for periprocedural stroke tended to increase in CAS, compared to CEA (odds ratio [OR], 1.86; 95% credible interval [CrI], 0.62–4.54). However, estimates for periprocedural myocardial infarction (MI) were quite heterogeneous in RCTs and NRCSs. Despite a trend of decreased risk with CAS in RCTs (OR, 0.70; 95% CrI, 0.27–1.24), the risk was similar in NRCSs (OR, 1.02; 95% CrI, 0.87–1.18). In indirect comparisons of MT and CAS, MT showed a tendency to have a higher risk for the composite of periprocedural death, stroke, MI, or nonperiprocedural ipsilateral stroke (OR, 1.30; 95% CrI, 0.74–2.73). Analyses of study characteristics showed that CEA-versus-MT studies took place about 10-year earlier than CEA-versus-CAS studies. Conclusions: A similar risk for periprocedural MI between CEA and CAS in NRCSs suggested that concerns about periprocedural MI accompanied by CEA might not matter in real-world practice when preoperative evaluation and management are working. Maybe the benefits of CAS over MT have been overestimated considering advances in medical therapy within10-year gap between CEA-versus-MT and CEA-versus-CAS studies.

A Novel ‘Bailout Switch Crush Technique’ for a Jailed Side Branch Unable to Rewire

Jae-Hyung Roh,Yong-Hoon Yoon,Minsu Kim,Jin Kyung Oh,Jae-Hwan Lee 대한심장학회 2021 Korean Circulation Journal Vol.51 No.11

The modified balloon crush technique : A simplified approach to optimizing final kissing balloon inflation

Roh, Jae-Hyung,Kim, Young-Hak,Kang, Hee Jun,Lee, Pil Hyung,Yoon, Sung-Han,Chang, Mineok,Ahn, Jung-Min,Park, Duk-Woo,Kang, Soo-Jin,Lee, Seung-Whan,Lee, Cheol Whan,Park, Seong-Wook,Park, Seung-Jung Williams & Wilkins Co 2018 Medicine Vol.97 No.42

<P><B>Abstract</B></P><P>This study aimed to investigate the feasibility and safety of the modified balloon crush technique.</P><P>The conventional crush technique accompanies difficult and suboptimal final kissing balloon inflation (FKI)</P><P>In a single-center percutaneous coronary intervention registry, 515 patients with 515 bifurcation lesions were treated with the modified balloon technique (n = 70) or the conventional crush technique (n = 445). In contrast to the conventional crush technique, where the implanted side branch (SB) stent is crushed by expansion of the main branch (MB) stent, the modified balloon crush technique uses balloon crushing and additional SB ballooning across the crushed SB stent before MB stenting to facilitate FKI. The primary outcome of interest was major adverse cardiovascular event (MACE), a composite of all-cause death, spontaneous myocardial infarction, and target vessel revascularization.</P><P>Baseline clinical and angiographic characteristics were similar between the 2 treatment groups. FKI had comparable success rates in both groups (97.1% for the modified balloon group and 98.4% for the conventional crush group; <I>P</I> = .35). There were no differences in procedure time, fluoroscopic time, or contrast amount between the 2 groups. At 1-year follow up, the cumulative MACE incidences were comparable between the 2 groups (7.3% vs 8.8%; <I>P</I> = .73). The incidence of target lesion revascularization (TLR) was significantly lower after the modified balloon crush technique compared with the conventional crush technique (0% vs 5.6%; <I>P</I> = .048).</P><P>The modified balloon crush technique appears to be a feasible and safe alternative to the conventional crush technique with the potential to reduce the revascularization rate.</P>

Fate of Grafts Bypassing Nonischemic Versus Ischemic Inducing Coronary Stenosis

Roh, Jae-Hyung,Kim, Young-Hak,Yang, Dong Hyun,Han, Seungbong,Yun, Sung-Cheol,Yang, Dong Heon,Park, Gyung-Min,Lee, Pil Hyung,Ahn, Jung-Min,Kang, Joon-Won,Lim, Tae-Hwan,Moon, Dae Hyuk,Kim, Joon Bum,Jung Elsevier 2018 The American Journal of Cardiology Vol.122 No.7

<P>There is a lack of evidence regarding the efficacy of ischemia-guided coronary artery bypass grafting. We compared the incidence of graft failure between grafts bypassing ischemia-inducing and nonischemia-inducing stenoses. Between 1997 and 2011, 2,304 patients for whom baseline coronary angiography and myocardial perfusion imaging were available were identified from a single-center coronary artery bypass grafting registry. According to baseline myocardial perfusion imaging, each graft was assigned to either graft bypassing ischemia-inducing or nonischemia-inducing stenoses (ischemia-related grafts, n = 4,904; ischemia-unrelated grafts, n = 2,709). Graft failure was defined as total occlusion on coronary computed tomography angiography, performed at the discretion of the treating physician. The incidence of graft failure was compared on a per-graft basis. At 5 years, the incidence of graft failure was significantly higher in the ischemia-unrelated grafts (4.2% vs 2.9% in ischemia-related grafts; p = 0.003). Ischemia-related graft was an independent determinant of graft patency (adjusted hazard ratio 0.61; 95% confidence interval 0.44 to 0.84; p = 0.002). Increased risk of graft failure associated with ischemia-unrelated graft was observed only in the internal thoracic artery (3.3% vs 2.0%, p = 0.021) and arterial grafts (6.5% vs 4.3%, p = 0.020), but not in the venous grafts (2.7% vs 2.7%; p = 0.99). In terms of major adverse cardiac and cerebrovascular events, 5-year incidences were comparable between the patients with and without ischemia-unrelated grafts (219, 19.3% vs 160, 18.0%; p = 0.61). In conclusion, ischemia-unrelated grafts became dysfunctional more frequently than ischemia-related grafts, and were not preventive of adverse events.</P>

Antibacterial activities of bark extracts from Fraxinus rhynchophylla Hance and Geranium koreanum Kom. against clinical strains of Clostridium perfringens in chickens

Oh, Jae-Young,Lee, Kwang-Jae,Wei, Bai,Roh, Jae-Hee,Kang, Min,Cha, Se-Yeoun,Jang, Hyung-Kwan The Korean Society of Veterinary Science 2015 大韓獸醫學會誌 Vol.55 No.2

Necrotic enteritis (NE) caused by Clostridium (C.) perfringens commonly occurs in domestic broiler farms since antibiotic supplementation in poultry feed has been banned. We evaluated the antibacterial activities of medicinal plant extracts against C. perfringens isolates to select alternative compounds for preventing NE. We compared antibacterial activities using two methods and evaluated susceptibilities of the isolates based on minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Two (Fraxinus rhynchophylla Hance [FRH] and Geranium koreanum Kom. [GKK]) of the 30 plant extracts had potent antibacterial activities against C. perfringens ATCC 13124 in two assays. The MIC values for FRH and GKK against 20 C. perfringens isolates were $128{\sim}256{\mu}g/mL$ and $32{\sim}128{\mu}g/mL$, respectively. The geometric MIC mean values for the two extracts were $147.2{\mu}g/mL$ and $68.8{\mu}g/mL$, respectively. The MBCs for the two extracts against the same strains were $1,024{\sim}2,048{\mu}g/mL$ and $256{\sim}1,024{\mu}g/mL$, respectively. The geometric mean MIC and MBC for GKK were about two-fold lower than those of FRH. The modified spot-on-lawn assay may be useful for measuring primary antibacterial potential. FRH and GKK are expected to be used as feed additives to prevent or treat NE in veterinary practice.

분산형전원의 전력계통 인터페이스 문제와 해결 방안

노재형(Jae Hyung ROH),신영균(Young-Kyun SHIN),김발호(Balho. H. KIM),김창섭(Chang-Sup KIM) 대한전기학회 2001 대한전기학회 학술대회 논문집 Vol.2001 No.7