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      • KCI등재후보

        Príncipes encobertos e princesas enamoradas: do disfarce por amor no teatro de Gil Vicente

        Maria João Pais do Amaral 한국 포르투갈-브라질 학회 2008 포르투갈-브라질 연구 Vol.5 No.1

        The main object of this essay is love disguise in two plays of Gil Vicente, Portuguese dramatist who developed his theatrical activity between 1502 and 1536 under the patronage of the monarchs Manuel I and João III. Widely spread in 16th century European literature, the motif of love disguise has its origins in chivalry romances, which are one of the many diverse sources of Gil Vicente's court theatre and also one of the dramatist's first spectators' favourite kind of books. I analyse it in Comédia do Viúvo and Tragicomédia de D. Duardos, where the protagonists, princes Rosvel and Duardos, hide their true identity and social rank from their beloved in order to prove the authenticity of their affection. In a first moment, I compare the way each of these two princes uses love disguise, reflecting on the function and meaning of the obvious differences this comparative analysis brings into light. In a second moment, I relate those differences with both the dramatic genre each of these two plays belongs and the conception of love that is on the basis of the stories enacted in Viúvo and Duardos, which, although originating in courtly love conventions, goes far beyond it. Finally, and because Gil Vicente's theatre is an art that strongly depends on circumstances, the date on which these spectacles were presented for the first time before the Portuguese king and court, as well as the purposes they were meant to fulfil, are also taken into consideration in this reflection on the innovative use of love disguise motif by one of the most important Portuguese dramatists of all times. The main object of this essay is love disguise in two plays of Gil Vicente, Portuguese dramatist who developed his theatrical activity between 1502 and 1536 under the patronage of the monarchs Manuel I and João III. Widely spread in 16th century European literature, the motif of love disguise has its origins in chivalry romances, which are one of the many diverse sources of Gil Vicente's court theatre and also one of the dramatist's first spectators' favourite kind of books. I analyse it in Comédia do Viúvo and Tragicomédia de D. Duardos, where the protagonists, princes Rosvel and Duardos, hide their true identity and social rank from their beloved in order to prove the authenticity of their affection. In a first moment, I compare the way each of these two princes uses love disguise, reflecting on the function and meaning of the obvious differences this comparative analysis brings into light. In a second moment, I relate those differences with both the dramatic genre each of these two plays belongs and the conception of love that is on the basis of the stories enacted in Viúvo and Duardos, which, although originating in courtly love conventions, goes far beyond it. Finally, and because Gil Vicente's theatre is an art that strongly depends on circumstances, the date on which these spectacles were presented for the first time before the Portuguese king and court, as well as the purposes they were meant to fulfil, are also taken into consideration in this reflection on the innovative use of love disguise motif by one of the most important Portuguese dramatists of all times.

      • KCI등재

        Molecular characterization of Escherichia coli O157:H7 strains isolated from different sources and geographic regions

        Adriana Hamond Regua-Mangia,Alice Gonçalves M. Gonzalez,Aloysio M. F. Cerqueira,João Ramos C.Andrade 대한수의학회 2012 Journal of Veterinary Science Vol.13 No.2

        Escherichia (E.) coli serotype O157:H7 is a globally distributed human enteropathogen and is comprised of microorganisms with closely related genotypes. The main reservoir for this group is bovine bowels, and infection mainly occurs after ingestion of contaminated water and food. Virulence genetic markers of 28 O157:H7 strains were investigated and multilocus enzyme electrophoresis (MLEE) was used to evaluate the clonal structure. O157:H7 strains from several countries were isolated from food, human and bovine feces. According to MLEE, O157:H7 strains clustered into two main clonal groups designated A and B. Subcluster A1 included 82% of the O157:H7 strains exhibiting identical MLEE pattern. Most enterohemorrhagic E. coli (EHEC) O157:H7 strains from Brazil and Argentina were in the same MLEE subgroup. Bovine and food strains carried virulence genes associated with EHEC pathogenicity in humans.

      • KCI등재

        Antifungal Activity of Silver Salts of Keggin-Type Heteropolyacids Against Sporothrix spp.

        Luciana Da Silva Mathias,João Carlos De Aquino Almeida,Luis César Passoni,Cristiani Miranda David Gossani,Gabriel Bonan Taveira,Valdirene Moreira Gomes,Olney Vieira-Da-Motta 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.4

        Sporotrichosis is a chronic and subacute mycosis causing epidemiological outbreaks involving sick cats and humans in southeastern Brazil. The systemic disease prevails in cats and in humans, with the symptoms restricted to the skin of immunocompetent individuals. Under these conditions, the prolonged treatment of animals and cases of recurrence justify the discovery of new treatments for sporotrichosis. This work addresses the antifungal activity of silver salts of Keggin-type heteropolyacid salts (Ag-HPA salts) such as Ag3[PW12O40], Ag6[SiW10V2O40], Ag4[SiW12O40] and Ag3[PMo12O40] and interactions with the antifungal drugs itraconazole (ITC), terbinafine (TBF) and amphotericin B (AMB) on the yeast and mycelia forms of Sporothrix spp. Sporothrix spp. yeast cells were susceptible to Ag-HPA salts at minimum inhibitory concentration (MIC) values ranging from 8 to 128 μg/ml. Interactions between Ag3[PW12O40] and Ag3[PMo12O40] with itraconazole and amphotericin B resulted in higher antifungal activity with a reduction in growth and melanization. Treated cells showed changes in cell membrane integrity, vacuolization, cytoplasm disorder, and membrane detachment. Promising antifungal activity for treating sporotrichosis was observed for the Ag-HPA salts Ag3[PMo12O40] and Ag3[PW12O40], which have a low cost, high yield and activity at low concentrations. However, further evaluation of in vivo tests is still required.

      • KCI등재

        Hybrid Wire-Arc Additive Manufacturing of Conformal Cooling Channels: A Feasibility Study

        João P. M. Pragana,Ivo M. F. Bragança,Carlos M. A. Silva,Paulo A. F. Martins 한국정밀공학회 2023 International Journal of Precision Engineering and Vol.10 No.1

        This paper investigates the feasibility of hybridizing wire-arc additive manufacturing with metal forming to create conformal cooling channels in moulds for hot material processing. An axisymmetric mould is used as a prototype test case and plastic deformation by flaring of the additively deposited inner tubular element is utilized to create the overhangs for connecting with the outer tubular element that are needed to fabricate the cooling channels. Finite element modelling of plastic deformation by flaring of the additively deposited material is carried out to identify the different modes of deformation and to determine the maximum allowable radius of the overhangs. Results show that the proposed hybridization of wire-arc additive manufacturing with plastic deformation by flaring adds flexibility to the design and fabrication of conformal channels in the as-built mould by eliminating the use of support structures and avoiding the use of complex deposition strategies. Finishing of the functional surfaces of the as-built mould by turning ensures the required accuracy and surface quality.

      • A General Template to Configure Multi-Criteria Problems in Ubiquitous GDSS

        João Carneiro,Diogo Martinho,Goreti Marreiros,Paulo Novais 보안공학연구지원센터 2015 International Journal of Software Engineering and Vol.9 No.11

        The study of multi-criteria problems adapted to the context of Ubiquitous Group Deci-sion Support Systems (UbiGDSS) is covered in the literature through very different per-spectives and interests. There are scientific studies related to the multi-criteria problems that lie across argumentation-based negotiation, multi-agent systems, dialogues, etc. However, to perform most of these studies, a high amount of information is required. The usage of so much data or information that is difficult to collect or configure can bring good results in theoretical scenarios but can be impossible to use in the real world. In order to overcome these issues, we present in this paper a general template to configure multi-criteria problems adapted for the contexts of UbiGDSS that intends to be easy and fast to configure, appellative, intuitive, permits to collect a lot of data and helps the deci-sion-maker transmitting his beliefs and opinions to the system. Our proposal includes three sections: Problem Data, Personal Configuration and Problem Configuration. We have developed a prototype with our template with the purpose to simulate the configura-tion of a multi-criteria problem. We invited real decision-makers to use our prototype in a simulated scenario and asked to them to fulfil a survey in the end in order to study our hypotheses. Our general template achieved good results and proved to be very percepti-ble and fast to configure.

      • KCI등재

        A laminated composite plate finite element a-priori corrected for locking

        João Elias Abdalla Filho,Ivan Moura Belo,Michele Schunemann Pereira 국제구조공학회 2008 Structural Engineering and Mechanics, An Int'l Jou Vol.28 No.5

        A four-node plate finite element for the analysis of laminated composites which is developed using strain gradient notation is presented. The element is based on a first-order shear deformation theory and on the equivalent lamina assumption. Strains and stresses can be calculated at different points through the thickness of the plate. They are averaged values due to the equivalent lamina assumption. A shear correction factor is used as the transverse shear strain is taken to be constant over the plate thickness while its actual variation is parabolic. Strain gradient notation, which is physically interpretable, allows for the detailed a-priori analysis of the finite element model. The polynomial expansions are inspected and spurious terms responsible for modeling errors are identified in the shear strains polynomial expansions. The element is corrected by simply removing the spurious terms from the shear strains expansions. The element is implemented into a FORTRAN finite element code in two versions; namely, with and without spurious terms. Results are compared to show the effects of the spurious terms on the solutions. It is also shown that a refined mesh composed of corrected elements provides solutions which approximate very well the analytical solutions, validating the procedure.

      • KCI등재

        Evaluation of the Contribution of Masonry Infill Panels on the Seismic Behaviour of Two Existing Reinforced Concrete Buildings

        João Milheiro,Hugo Rodrigues,António Arêde 대한토목학회 2016 KSCE Journal of Civil Engineering Vol.20 No.4

        Reinforced concrete frames infilled with masonry panels without proper seismic design can protect or potentiate the risk of structures when subject to earthquakes. This paper presents two case studies of existing reinforced concrete buildings with masonry infill panels located in Portugal in different seismic zones according with the EuroCode 8. The RC buildings were studied with and without the infill panels using non-linear dynamic analysis, comparing the different seismic response in terms of global storey displacements, inter-storey drifts, storey shear and ductility demands in the columns. The results show that in one building it is safe and conservative not to take the panels into account, however in the case with irregular infill distribution in elevation the results are significantly different and the presence of the infills plays and important role.

      • UbiGDSS : A Theoretical Model to Predict Decision-Makers’ Sat-isfaction

        João Carneiro,Ricardo Santos,Goreti Marreiros,Paulo Novais 보안공학연구지원센터 2015 International Journal of Multimedia and Ubiquitous Vol.10 No.7

        The market globalization and the firms’ internationalization hinder the matching of the top managers’ agenda, making it difficult to meet in the same space or time. On the one hand, the appearance of Ubiquitous Group Decision Support Systems (UbiGDSS) ena-bled individuals to gather and make decisions in different spaces at different times, but on the other hand, originated problems related to the lack of human interaction. To under-stand how the arguments used can influence each of the decision-makers, what is their satisfaction regarding the decision made, and other affective issues such as emotions and mood, are some examples of that lack. In order to try to overcome this lack, we propose a theoretical model that is specially designed for agents, helping to understand the interac-tions impact on each agent and their satisfaction with the decision made.

      • KCI등재

        Quantitative real-time polymerase chain reaction for detecting Mycoplasma hyosynoviae and Mycoplasma hyorhinis in pen-based oral, tonsillar, and nasal fluids

        João Carlos Gomes Neto,Leslie Bower,Barbara Z. Erickson,Chong Wang,Matthew Raymond,Erin L. Strait 대한수의학회 2015 Journal of Veterinary Science Vol.16 No.2

        Mycoplasma (M.) hyorhinis and M. hyosynoviae are pathogens known to cause disease in pigs post-weaning. Due to their fastidious nature, there is increased need for culture-independent diagnostic platforms to detect these microorganisms. Therefore, this study was performed to develop and optimize quantitative real-time PCR (qPCR) assays to rapidly detect M. hyorhinis and M. hyosynoviae in pen-based oral fluids as well as nasal and tonsillar fluids as proxies for samples used in swine herd surveillance. Two methods of genomic DNA extraction, automated versus manual, were used to compare diagnostic test performance. A wean-to-finish longitudinal study was also carried out to demonstrate the reproducibility of using pen-based oral fluids. Overall, pen-based oral and tonsillar fluids were more likely to be positive for both types of bacteria whereas only M. hyorhinis was detected in nasal fluids. DNA extraction protocols were shown to significantly influence test result. Although the initial detection time somewhat differed, both organisms were repeatedly detected in the longitudinal study. Overall, this study evaluated two qPCR methods for rapid and specific detection of either mycoplasma. Results from the present investigation can serve as a foundation for future studies to determine the prevalence of the two microorganisms, environmental load, and effectiveness of veterinary interventions for infection control.

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