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Gonzalez-Perez, C.J.,Vargas-Arispuro, I.,Aispuro-Hernandez, E.,Aguilar-Gil, C.L.,Aguirre-Guzman, Y.E.,Castillo, A.,Hernandez-Mendoza, A.,Ayala-Zavala, J.F.,Martinez-Tellez, M.A. The Korean Society for Microbiology and Biotechnol 2019 한국미생물·생명공학회지 Vol.47 No.2
The consumption of fresh vegetables has been related to recurrent outbreaks of foodborne diseases (FBD) worldwide. Therefore, the development of effective alternative technologies is necessary to improve the safety of these products. This study aimed to isolate and identify epiphytic lactic acid bacteria (LAB) from fresh fruits and leafy vegetables and characterize their antagonistic capacity due to their ability to produce bacteriocins or antibacterial compounds. For this, 92 LAB isolates from fruits and leafy vegetables were screened for antagonistic activity. Two strains with the highest and broadest antagonistic activities were selected for further characterization; one from cantaloupe melon (strain CM175) and one from cilantro leaves (strain C15). The cell-free supernatants (CFS) of CM175 and C15 were found to exhibit antagonistic activity against FBD-causing pathogens. The CM175 and C15 strains were identified as Pediococcus pentosaceus and Lactobacillus graminis, respectively. Notably, the P. pentosaceus CM175 CFS stopped the growth of Salmonella Typhimurium, Salmonella Saintpaul, Staphylococcus aureus, and Listeria monocytogenes, and delayed Escherichia coli O157:H7 growth. Moreover, L. graminis C15 CFS delayed the growth of all indicator pathogens, but did not completely stop it. Organic acids and bacteriocin-like molecules were determined to be possibly exerting the observed antagonistic activity of the identified LAB strains. Thus, application of the antagonistic compounds produced by Pediococcus pentosaceus and Lactobacillus graminis could be a novel and ecological strategy in developing antimicrobial biopreservatives for the food industry and mitigating FBD by reducing the biological contamination in fruit and vegetable orchards, mainly via their potential in controlling both gram-negative and gram-positive pathogenic bacteria.
( C. J. Gonzalez-perez ),( I. Vargas-arispuro ),( E. Aispuro-hernandez ),( C. L. Aguilar-gil ),( Y. E. Aguirre-guzman ),( A. Castillo ),( A. Hernandez-mendoza ),( J. F. Ayala-zavala ),( M. A. Martinez 한국미생물생명공학회(구 한국산업미생물학회) 2019 한국미생물·생명공학회지 Vol.47 No.2
The consumption of fresh vegetables has been related to recurrent outbreaks of foodborne diseases (FBD) worldwide. Therefore, the development of effective alternative technologies is necessary to improve the safety of these products. This study aimed to isolate and identify epiphytic lactic acid bacteria (LAB) from fresh fruits and leafy vegetables and characterize their antagonistic capacity due to their ability to produce bacteriocins or antibacterial compounds. For this, 92 LAB isolates from fruits and leafy vegetables were screened for antagonistic activity. Two strains with the highest and broadest antagonistic activities were selected for further characterization; one from cantaloupe melon (strain CM175) and one from cilantro leaves (strain C15). The cell-free supernatants (CFS) of CM175 and C15 were found to exhibit antagonistic activity against FBD-causing pathogens. The CM175 and C15 strains were identified as Pediococcus pentosaceus and Lactobacillus graminis, respectively. Notably, the P. pentosaceus CM175 CFS stopped the growth of Salmonella Typhimurium, Salmonella Saintpaul, Staphylococcus aureus, and Listeria monocytogenes, and delayed Escherichia coli O157:H7 growth. Moreover, L. graminis C15 CFS delayed the growth of all indicator pathogens, but did not completely stop it. Organic acids and bacteriocin-like molecules were determined to be possibly exerting the observed antagonistic activity of the identified LAB strains. Thus, application of the antagonistic compounds produced by Pediococcus pentosaceus and Lactobacillus graminis could be a novel and ecological strategy in developing antimicrobial biopreservatives for the food industry and mitigating FBD by reducing the biological contamination in fruit and vegetable orchards, mainly via their potential in controlling both gram-negative and gram-positive pathogenic bacteria.
Power Absorption Measurements during NMR Experiments
N. Felix-Gonzalez,A. L. Urbano-Bojorge,C. Sanchez-L de Pablo,V. Ferro-Llanos,F. del Pozo-Guerrero,J. J. Serrano-Olmedo 한국자기학회 2014 Journal of Magnetics Vol.19 No.2
The heating produced by the absorption of radiofrequency (RF) has been considered a secondary undesirable effect during MRI procedures. In this work, we have measured the power absorbed by distilled water, glycerol and egg-albumin during NMR and non-NMR experiments. The samples are dielectric and examples of different biological materials. The samples were irradiated using the same RF pulse sequence, whilst the magnetic field strength was the variable to be changed in the experiments. The measurements show a smooth increase of the thermal power as the magnetic field grows due to the magnetoresistive effect in the copper antenna, a coil around the probe, which is directly heating the sample. However, in the cases when the magnetic field was the adequate for the NMR to take place, some anomalies in the expected thermal powers were observed: the thermal power was higher in the cases of water and glycerol, and lower in the case of albumin. An ANOVA test demonstrated that the observed differences between the measured power and the expected power are significant.
Baryon content of massive galaxy clusters at 0.57 < <i>z</i> < 1.33
Chiu, I.,Mohr, J.,McDonald, M.,Bocquet, S.,Ashby, M. L. N.,Bayliss, M.,Benson, B. A.,Bleem, L. E.,Brodwin, M.,Desai, S.,Dietrich, J. P.,Forman, W. R.,Gangkofner, C.,Gonzalez, A. H.,Hennig, C.,Liu, J. Oxford University Press 2016 MONTHLY NOTICES- ROYAL ASTRONOMICAL SOCIETY Vol.455 No.1
Improved Neutron Capture Cross Section Measurements with the n_TOF Total Absorption Calorimeter
E. Mendoza,V. Becares,A. Casado,D. Cano-Ott,M. Fernandez-Ordonez,E. Gonzalez-Romero,C. Guerrero,T. Martinez,J. J. Vidriales,J. Andrzejewski,J. Marganiec,J. Perkowski,L. Audouin,B. Berthier,L. Tassan-G 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.23
The n_TOF collaboration operates a Total Absorption Calorimeter (TAC) [1] for measuring neutron capture cross-sections of low-mass and/or radioactive samples. The results obtained with the TAC have led to a substantial improvement of the capture cross sections of ^(237)Np and ^(240)Pu [2].The experience acquired during the first measurements has allowed us to optimize the performance of the TAC and to improve the capture signal to background ratio, thus opening the way to more complex and demanding measurements on rare radioactive materials. The new design has been reached by a series of detailed Monte Carlo simulations of complete experiments and dedicated test measurements. The new capture setup will be presented and the main achievements highlighted. KEYWORDS: ND2010, nuclear data, n\textunderscore TOF, background, Monte Carlo, neutron, time of flight, cross section, calorimeter, shielding, simulation, total absorption, gamma ray, neutron capture.
PPARα activation drives demethylation of the CpG islands of the Gadd45b promoter in the mouse liver
Kim, J.H.,Wahyudi, L.D.,Kim, K.K.,Gonzalez, F.J. Academic Press 2016 Biochemical and biophysical research communication Vol.476 No.4
Growth arrest and DNA damage-inducible beta (GADD45b) plays a pivotal role in many intracellular events in both cell survival- and cell death-related signaling. To date, the study of GADD35b has mainly focused on investigation of its function, as well as interacting molecules. However, studies of Gadd45b gene regulation are limited. In this study, we investigated the transcriptional regulation mechanism of Gadd45b. Since Gadd45b mRNA is highly induced by the PPARα agonist Wy-14,643 in the mouse liver, we analyzed the Gadd45b promoter using an in vivo reporter assay. Interestingly, the naked Gadd45b-luciferase construct strongly induced luciferase activity without any stimulant in our in vivo system. Therefore, we investigated the epigenetic changes in the Gadd45b promoter region using mouse liver genomic DNA, the methylation-specific restriction enzyme (HpaII), and disulfide conversion. Our results showed that two possible CpG methylation sites were methylated and demethylated by Wy-14,643 treatment. This study indicates that epigenetic change at the Gadd45b promoter is critical for Gadd45b induction.
Cheymol, A.,Gonzalez Sotelino, L.,Lam, K. S.,Kim, J.,Fioletov, V.,Siani, A. M.,De Backer, H. Copernicus GmbH 2009 Atmospheric chemistry and physics Vol.9 No.2
<P>Abstract. The Langley plot method applied on the Brewer Ozone measurements can provide accurate Aerosol Optical Depth (AOD) in the UV-B. We present seven intercomparisons between AOD retrieved from Brewer Ozone measurements at 320 nm and AOD measured by CIMEL sunphotometer at 340 nm or 440 nm (shifted to 320 nm in using the Angström's law), which are stored in the international AERONET database. Only the intercomparisons between co-located instruments can be used to validate the Langley Plot Method applied to the Brewer measurements: in this case, all the correlation coefficients are above 0.82. If the instruments are not at the same site, the correlation between the AOD retrieved by both instruments is much lower. In applying the Angström's law the intercomparison is improved compared to previous study. </P>
A. Paschoa,J.L. Gonzalez,E.C. Passamani 한국물리학회 2020 Current Applied Physics Vol.20 No.7
Anisotropic Ginzburg-Landau model was systematically tested in NbtNb and NbtNb/Cu5nm/Co40nm (tNb = 20 and 100 nm) films. A ΔTC-parameter was defined as a temperature difference between the critical temperatures determined for in-plane and out-of-film plane field configurations (ΔTC = TC⊥C − TC∥C). The ΔTC behavior for Nb (20 nm) and Nb(20 nm)/Co(40 nm) films shows a linear increase with applied field concomitantly with the increase of magnetization values in the out-of-film plane configuration. The stray field of ferromagnetic Co layer was determined in the zero field case to be (7 ± 2) mT, while a variation ratio between the stray field and the applied field ((dBCo) dB of the Co layer was calculated to be (−0.22 ± 0.02). Our results bring contributions to clarify proximity and magnetostatic effects; issues that still need to be comprehended for superconducting spin valve technological applications.