Acanthoic acid, a diterpene in Acanthopanax koreanum, protects acetaminophen-induced hepatic toxicity in mice

Wu, Y.-L.,Jiang, Y.-Z.,Jin, X.-J.,Lian, L.-H.,Piao, J.-Y.,Wan, Y.,Jin, H.-R.,Joon Lee, J.,Nan, J.-X. G. Fischer 2010 Phytomedicine Vol.17 No.6

The protective effect of a diterpenoid acanthoic acid (AA) isolated from Acanthopanax koreanum Nakai was investigated in acetaminophen (APAP)-induced hepatic toxicity. Drug-induced hepatotoxicity induced by an intraperitoneal (i.p.) injection of 300mg/kg (sub-lethal dose) of APAP. Pretreatment with AA (50 and 100mg/kg) orally 2h before the APAP administration attenuated the APAP-induced acute increase in serum aspartate aminotransferase (AST), and alanine aminotransferase (ALT) activites, replenished the depleted hepatic glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) activities, decreased malondialdehyde (MDA) level and considerably reduced the histopathological alterations in a manner similar to silymarin (Sily). Immunohistochemical analyses also demonstrated that AA could reduce the appearance of necrosis regions as well as caspase-3 and hypoxia inducible factor-1α (HIF-1α) expression in liver tissue. Our results indicated that AA protected liver tissue from the oxidative stress elicites by APAP-induced liver damage and suggestes that the hepatic protection mechanism of AA would relate to antioxidation and hypoxia factor on APAP-induced hepatotoxicity.

Direct observation of imprinted antiferromagnetic vortex states in CoO/Fe/Ag(001) discs

Wu, J.,Carlton, D.,Park, J. S.,Meng, Y.,Arenholz, E.,Doran, A.,Young, A. T.,Scholl, A.,Hwang, C.,Zhao, H. W.,Bokor, J.,Qiu, Z. Q. Springer Science and Business Media LLC 2011 Nature physics Vol.7 No.4

Altered tooth morphogenesis after silencing the planar cell polarity core component, Vangl2

Wu, Z.,Epasinghe, D. J.,He, J.,Li, L.,Green, D. W.,Lee, M. J.,Jung, H. S. Springer Science + Business Media 2016 Cell and tissue research Vol.366 No.3

<P>Vangl2, one of the core components of the planar cell polarity (PCP) pathway, has an important role in the regulation of morphogenesis in several tissues. Although the expression of Vangl2 has been detected in the developing tooth, its role in tooth morphogenesis is not known. In this study, we show that Vangl2 is expressed in the inner dental epithelium (IDE) and in the secondary enamel knots (SEKs) of bell stage tooth germs. Inhibition of Vangl2 expression by siRNA treatment in in vitro-cultured tooth germs resulted in retarded tooth germ growth with deregulated cell proliferation and apoptosis. After kidney transplantation of Vangl2 siRNA-treated tooth germs, teeth were observed to be small and malformed. We also show that Vangl2 is required to maintain the proper pattern of cell alignment in SEKs, which maybe important for the function of SEKs as signaling centers. These results suggest that Vangl2 plays an important role in the morphogenesis of teeth.</P>

cDNA Cloning and Polymorphism of the Porcine Carbonic Anhydrase III (CA3) Gene

Wu, J.,Deng, Changyan,Xiong, Y.Z.,Zhou, D.H.,Lei, M.G.,Zuo, B.,Li, F.E.,Wang, J. Asian Australasian Association of Animal Productio 2006 Animal Bioscience Vol.19 No.3

Carbonic anhydrase III (CA3) is a member of a multigene family that encode carbonic anhydrase isozymes. In this study, a complete coding sequence of the pig CA3 gene which encodes a 260 amino-acid protein was determined. The amino acid comparison showed high sequence similarities with previously identified human (86.5%) CA3 gene and mouse (91.5%) Car3 gene. The partial genomic DNA sequences were also investigated. The length of intron 1 was 727 bp. Comparative sequencing of three pig breeds revealed that there was a T${\rightarrow}$C substitution at position 363 within intron 1. The substitution was situated within a NcoI recognition site and was developed as a PCR-restriction fragment length polymorphism (RFLP) marker for further use in population variation investigations and association analysis. Two alleles (A and B) were identified, and 617 bp fragments were observed for the AA genotype and 236 bp and 381 bp fragments for the BB genotype. The polymorphism of CA3 was detected in 8 pig breeds. Allele B was predominant in the Western pig breeds. In addition, association studies of the CA3 polymorphism with carcass traits in 140 $Yorkshire{\times}Meishan$ $F_2$ offspring showed that the NcoI PCR- RFLP genotype may be associated with variation in several carcass traits of interest for pig breeding. Allele B was associated with increases in lean meat percentage, loin eye height and loin eye area. Statistically significant association with backfat thickness was also found; pigs with the AB genotype had much less backfat thickness than AA or BB genotypes.

Coordinating strategic outsourcing decisions for semiconductor assembly using a bi-objective genetic algorithm

Wu, J. Z.,Chien, C. F.,Gen, M. Taylor Francis 2012 INTERNATIONAL JOURNAL OF PRODUCTION RESEARCH - Vol.50 No.1

The Updated Vversion of Chinese Evaluated Nuclear Data Library (CENDL-3.1)

Z. G. Ge,Z. X. Zhao,H. H. Xia,Y. X. Zhuang,T. J. Liu,J. S. Zhang,H. C. Wu 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.23

The updated Chinese Evaluated Nuclear Data Library CENDL-3.1 is an important achievement based on the nuclear data evaluation works in recent years, at China Nuclear Data Center (CNDC) in cooperation with China Nuclear Data Coordination Network (CNDCN). CENDL-3.1 contains the evaluated data for reactions with incident neutrons on 240 nuclides (from ^1H to ^(249)Cf) in energy region of 10^(-5) eV-20MeV. All data obtained according to the evaluations of experimental data and the predictions of the nuclear reaction model calculations. For most important nuclei of this library, the benchmark testing and validations have been performed, the comparisons with other nuclear data libraries (ENDF/B, JENDL, BROND, JEF, et al.) have been done. The testing version of CENDL-3.1 is CENDL-3.0, which has been provided for China domestic users. Follow the using back feed of CENDL-3.0, a lot of improvement has been done. The CENDL3.1 is provided for all users by ENDF format and is released to the world in the end of 2009. The overview, evaluation methodology and some important results of new evaluations, benchmark testing and validations for this library will be introduced in this contribution.

Experimental study of the x-ray linear dichroism of NiO films grown on Fe(001)

Wu, Y. Z.,Sinkovic, B.,Won, C.,Zhu, J.,Zhao, Y.,Qiu, Z. Q. American Physical Society 2012 Physical review. B, Condensed matter and materials Vol.85 No.13

Flow characteristics after water inrush from the working face in karst tunneling

Wu, J.,Li, S.C.,Xu, Z.H.,Pan, D.D.,He, S.J. Techno-Press 2018 Geomechanics & engineering Vol.14 No.5

In order to investigate flow characteristics after water inrush from the working face in process of karst tunnel construction, numerical calculation for two class case studies of water inrush is carried out by using the FLUENT software on the background of Qiyueshan tunnel. For each class water inrush from the tunnel face, five cases under different water-inrush velocity are simulated and researched. Three probing lines are selected respectively in the left tunnel, cross passage, right tunnel and in the height direction of the tunnel centerline. The variation characteristics of velocity and pressure on each probing line under the five water-inrush velocities are analyzed. As for the selected four groups probing lines in the tunnels, the change rules of velocity and pressure on each group probing lines under the same water-inrush velocity are discussed. Finally, the water flow characteristics after inrush from the tunnel face are summarized by comparing the case studies. The results indicate that: (1) The velocity and pressure change greatly at the intersection area of the cross passage and the tunnels. (2) The velocity nearby the tunnel side wall is the minimum, while it is the maximum in the middle position. (3) The pressure value of every cross section in the tunnels is basically fixed. (4) As water-inrush velocity increases, the flow velocity and pressure in the tunnels also increase. The former is approximately proportional to their respective water-inrush velocity, while the latter is not. The research results provide a theoretical basis for making scientific and rational escape routes.

Atomistic simulation analysis of the effects of void interaction on void growth and coalescence in a metallic system

Z.X. Wen,J.P. Wang,Y.W. Wu,K.J. Zhou,Z.F. Yue 한국물리학회 2018 Current Applied Physics Vol.18 No.6

Material deformation caused by the interaction between defects is a significant factor of material fracture failure. The present study employs molecular dynamics simulations of single-void and double-void crystalline Ni atomic systems to investigate inter-void interactions. Furthermore, simulations showing the evolution of dislocations for three different crystallographic orientations are conducted to study the void growth and coalescence. The simulations also consider the effect of the radius of the secondary void on dislocation evolution. The results show that double-void systems are more prone to yield than single-void systems. Further microstructural analysis indicates that the interaction between voids is realized by dislocation reactions. The simulation results of the dislocation evolution of the three orientations reveal that a relationship exists between the evolution of the dislocation density and the stress-strain curve. At the initial stage of dislocation, the dislocation grows slowly, and consists of Shockley partial dislocation. The dislocation growth rate then increases significantly in the sharply declining stage of the stress-strain curve, where most of dislocations are Shockley partial dislocation. Analysis of the dislocation length during the overall simulation indicates that the dislocation length of the [110] orientation is the longest, followed by that of the [111] orientation and the [100] orientation, which has the shortest dislocation length.

Fluorescent Realgar Quantum Dots: New Life for an Old Drug

J. Z. Wu,G. Chen,Y. B. Shao,J. Liu,Y. C. Sun,H. S. Lin,Paul C. Ho 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.1

"Quantum dots (QDs) possess unique properties that are expected for various applications. Herein, realgar (As4S4) QDs were synthesized via a wet chemical method in a coordinating solvent (e.g., ethanolamine (EA)) with assistance of an acid (e.g., citric acid (CA)). The asprepared realgar QDs using EA and CA have a narrow size distribution with a mean value of 5.48 nm and a relative standard deviation of 20%, and exhibit bright photoluminescence (PL) in a short wavelength window (< 480 nm) with high quantum yield up to 70%. Moreover, realgar QDs can retain their high PL after one-year storage, indicating their excellent stability. Bulk realgar is a commonly used traditional medicine, however, its water-insolubility limits its broad applications. Realgar QDs could be an alternative for the bulk realgar owing to their excellent water-solubility and efficient PL. In preliminary in vivo toxic and pharmaceutical experiments, the synthesized realgar QDs at a single oral dose of 10.0 mg realgar/kg body weight for 21 days showed tolerability in healthy mice and effective against tumor-bearing nude mice, respectively. In this study, assembly of realgar QDs into specific hollow nanospheres was first constructed by introducing an acid into their original colloidal solution. Simple dissolution of such assemblies in EA can partially return them into the fluorescent QDs. Such property is desired for a variety of media-responsive luminescence materials. In addition, a flexible inorganic/organic hybrid composite film was fabricated for the first time by incorporation of realgar QDs into polyvinyl alcohol matrix. The resulting film presents excellent PL, good transparency, intense mechanical strength and facile processibility, which meet the requirements for device integration. "