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김영기,Judah Arey Denburg 대한비과학회 1996 Journal of rhinology Vol.3 No.2
It is well known that the number of mucosal and/or stromal mast cells is increased in nasal polyps as compared with normal nasal mucosa ; these increase are suppressed by topical corticosteroid treatment. However, pathophysiology of mast cell accumulation in nasal polyp tissues and inhibition of this accumulation by steroid treatment have not yet clearly clarified. The aim of this study was to determine whether stem cell factor (SCF) expression is increased in nasal polyp and whether this decreases after steroid treatment. We performed Northern blot analyses of cultured nasal polyp epithelial cells and cultured nasal polyp fibroblasts. We also assessed SCF protein in nasal polyp epithelial conditioned medium (NpEpCM) and nasal polyp fibroblast conditioned medium (NpFbCM) by ELISA. To determine the biological activity of SCF protein in NpFbMC, we utilized a mast cell differentiation assay, employing human bone marrow-derived nonadherent mononuclear cells (BMMNC). To detect the expression of immunoreactive SCF protein in nasal polyp structural cells as well as before and after in vivo treatment of polyps with topical corticosteroids, we performed immunohistochemical staining for SCF in cultured nasal polyp epithelial cells, cultured nasal polyp fibroblasts and nasal polyp tissue sections from steroid treated and untreated patients. There was significant expression of SCF mRNA by cultured nasal polyp epithelial cells and cultured nasal polyp fibroblasts, as well as SCF protein in NpEpCM and NpFbCM. NpFbCM supported the differentiation of tryptase positive mast cells in vitro, an effect which was blocked completely by anti-rhSCF antibody. In steroid untreated nasal polyps immunoreactive SCF protein was detected in epithelial cells, fibroblasts and endothelial cells, which was significantly diminished in frequency and intensity after steroid treatment. The present study indicates that human nasal polyp structural cells express and produce SCF. The results of this study suggest that topical steroid treatment may suppress SCF expression in nasal polyps which may be responsible for decrease in mast cells and symptom improvement.