Molecular identification and virulence of six isolates of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) to Bemisia tabaci Q biotype

M.T. Islam,D. Omar,M. Shabanimofrad 한국응용곤충학회 2014 Journal of Asia-Pacific Entomology Vol.17 No.3

The aimof this studywas to investigate the molecular identification and virulence of the entomopathogenic fungusMetarhizium anisopliae. Initially, the alignments of nucleotide sequences of the internal transcribed spacer(ITS) 1-5.8S-ITS4 regions of six isolates–TFFH1, TFFH3, PR1, MAP, GT3, and GT2–were investigated using GenBankITS sequences for the same region and species of fungus. Thereafter, the in vitro (germination, vegetative growth,and sporulation) and the in vivo (%mortality of Bemisia tabaci) virulence of the funguswere investigated. A BLASTsearch of the ITS1 region revealed a match to a sequence registered under the accession number forM. anisopliae. The submitted sequence datawere registered and providedwith the accession numbers JX041507 to JX0415012,which were released in August 2012 (GenBank). In the virulence experiments, the highest germination percentage,vegetative growth, and sporulation of M. anisopliae were observed in the isolate PR1, with mean values of97.2%, 4.6 cm, and 7.2 × 106 conidia/ml, respectively; while the highest mortality percentage of B. tabaci was observedin the isolate GT3,with an mean value of 84.3%. This study concludes that all the six tested isolates–TFFH1,TFFH3, PR1, MAP, GT3, and GT2–are M. anisopliae and the isolates PR1 and GT3 are more virulent to B. tabaci thanthat by the other four isolates.

Synthesis, Characterization, and Reactivity of Novel 6H-1,3,5-Oxathiazine S,S-Dioxides

Islam, M. R.,Takikawa, Y.,Lim, K. T. Wiley-Blackwell 2014 Journal of heterocyclic chemistry Vol.51 No.3

A series of novel 6H-1,3,5-oxathiazine S,S-dioxides were synthesized by the m-CPBA oxidation (2.2 equiv) of 6H-1,3,5-oxathizines. The synthetic utilities of the newly synthesized cyclic sulfones were investigated. In a thermal condition, compounds 6H-1,3,5-oxathiazine S,S-dioxides were found relatively stable, but Lewis acid-induced thermal reaction afforded the corresponding amides. The plausible pathway to amides from 6H-1,3,5-oxathiazine S,S-dioxides was also discussed in this account.

Type of tooth movement during en masse retraction of the maxillary anterior teeth using labial versus lingual biocreative therapy in adults: A randomized clinical trial

Mais M. Sadek,Noha E. Sabet,Islam T. Hassan 대한치과교정학회 2019 대한치과교정학회지 Vol.49 No.6

Objective: The objective of this two-arm parallel trial was to compare the type of tooth movement during en masse retraction of the maxillary anterior teeth using labial versus lingual biocreative therapy. Methods: Twenty-eight subjects were randomized in a 1 : 1 ratio to either the labial or lingual group. En masse anterior retraction was performed using labial biocreative therapy in group A and lingual biocreative therapy in group B. Cone beam computed tomography scans were taken before and after retraction and the primary outcome was the type of tooth movement during anterior retraction. Data were analyzed using paired t -tests for comparisons within each group and independent-sample t-test for comparison of the mean treatment changes between the two groups. Results: Significant differences were found between the two groups in relation to the type of tooth movement (labiolingual inclination of the central incisor; mean difference, 5.85 ± 1.85o). The canine showed significant distal tipping in the lingual group (mean difference, 6.98 ± 1.25o). The canine was significantly more intruded in the lingual group (mean difference, 1.67 ± 0.49 mm). Good anchorage control and significant soft tissue changes occurred in both groups. No serious adverse effects were detected. Conclusions: With a 10-mm retraction hook, the labial biocreative technique with the reverse curve overlay provided anterior retraction with good torque control, while in the lingual group, anterior retraction occurred with controlled tipping movement with significant distal tipping and intrusion of the canine (trial registration: The trial was registered at ClinicalTrials.gov [NCT03239275]).

Adventitious Shoot regeneration From Cotyledon in Aegle marmelos Corr.

M. R. Islam(M.R. 이슬람),S. Zaman(S. 자만),T.M. Sa(사동민) 한국토양비료학회 2006 한국토양비료학회 학술발표회 초록집 Vol.2006 No.10

Immune response and efficacy of pigeon pox virus vaccine and fowl pox virus vaccine in chickens

Shil, Niraj Kanti,Rahman, Md Siddique,Hossain, M.T.,Islam, M.T.,Rahman, M.M.,Islam, K.B.M. Saiful,Cha, Se-Yeoun,Jang, Hyung-Kwan,Song, Hee-Jong The Korean Society of Veterinary Service 2007 韓國家畜衛生學會誌 Vol.30 No.4

The humoral immune response of chicken vaccinated with fowl and pigeon pox virus vaccines was determined with the protective potentiality of the two vaccines in field condition of Bangladesh. Different aged Fayoumi chicks were subjected for the study. To assess the relationship with better immune response among experimental groups, the average percentage of 'take reaction' was examined and recorded to 97.77% in group A, 93.33% in group B and 100.0% in group C. The level of immune status induced by different vaccinated group was measured by passive hemagglutination (PHA) microplate test method. The mean PHA titer levels after primary vaccination were $33.06{\pm}14.13$ in group A, $32.0{\pm}14.81$ in group B, and $33.0{\pm}13.66$ in group C. Following booster vaccination, the mean PHA titer levels in prior of challenge were increased to $55.46{\pm}14.64$ in groups A and C, and $46.93{\pm}16.52$ in group B. The recorded PHA titer levels of each group at two weeks after challenge were significantly increased to $106.66{\pm}31.22$, $93.86{\pm}33.04$ and $110.93{\pm}29.29$, respectively. The PHA titer levels after vaccination and challenge were significantly increased compared to pre-vaccination titer levels (P<0.01). Although the PHA titer levels among three groups administrated different vaccine combinations in prior of challenge were significantly varied (P<0.01), it was observed that all of the vaccinated chicks were highly protected against challenge infection.

Effect of Sea Tangle (Laminaria japonica) and Charcoal Supplementation as Alternatives to Antibiotics on Growth Performance and Meat Quality of Ducks

Islam, M.M.,Ahmed, S.T.,Kim, Y.J.,Mun, H.S.,Kim, Y.J.,Yang, C.J. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.2

A total of 150 growing ducks were assigned to five dietary treatments to study the effect of sea tangle and charcoal (STC) supplementation on growth performance and meat characteristics in a completely randomized design. There were six replicates and five ducklings in each replication. The five dietary treatments were control, antibiotic, and 0.1%, 0.5%, and 1% STC supplemented diets. No significant differences were found on ADG, ADFI, and gain:feed among treatments in different weeks. The overall (0 to 3 weeks) ADFI decreased in antibiotic treatment (p<0.05) whereas the gain:feed increased significantly upon 1.0% STC supplementation compared to control (p<0.05). No significant variation was found in meat chemical composition except crude fat content which was high in 1.0% STC dietary group (p<0.05). Meat cholesterol was reduced in 0.1% STC group (p<0.05) compared to other dose levels while serum cholesterol was unaffected. High density lipoprotein (HDL) content was high in 1.0% STC (p<0.05) and low density lipoprotein (LDL) was low in 0.1% and 1.0% STC dietary groups (p = 0.06). No significant effect was found on the thiobarbituric acid reactive substances (TBARS) of fresh meat, whereas the TBARS value of meat preserved for 1 week was reduced significantly in STC dietary groups (p<0.05). The 0.1% STC dietary group showed an increased myristic acid (p = 0.07) content whereas, the content of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids increased in STC supplementation than antibiotic group (p<0.05). An increased concentration of omega-3 fatty acids and a reduced ratio of n-6/n-3 PUFA ratio was found upon 1.0% STC supplementation compared to antibiotic dietary group (p<0.05). Therefore, 1.0% STC dietary supplementation can be used as alternatives to antibiotics in duck production.

A high affinity kidney targeting by chitobionic acid-conjugated polysorbitol gene transporter alleviates unilateral ureteral obstruction in rats

Islam, M.A.,Kim, S.,Firdous, J.,Lee, A.Y.,Hong, S.H.,Seo, M.K.,Park, T.E.,Yun, C.H.,Choi, Y.J.,Chae, C.,Cho, C.S.,Cho, M.H. IPC Science and Technology Press 2016 Biomaterials Vol.102 No.-

<P>Aside from kidney transplantation a procedure which is exceedingly dependent on donor-match and availability leading to excessive costs there are currently no permanent treatments available which reverse kidney injury and failure. However, kidney-specific targeted gene therapy has outstanding potential to treat kidney-related dysfunction. Herein we report a novel kidney-specific targeted gene delivery system developed through the conjugation of chitobionic acid (CBA) to a polysorbitol gene transporter (PSGT) synthesized from sorbitol diacrylate and low molecular weight polyethylenimine (PEI) carrying hepatocyte growth factor (HGF) gene to alleviate unilateral ureteral obstruction (UUO) in rats. CBA-PSGT performed exceptionally well for targeted delivery of HGF to kidney tissues compared to its non-targeted counterparts (P < 0.001) after systemic tail-vein injection and significantly reduced the UUO symptoms, returning the UUO rats to a normal health status. The kidney-targeted CBA-PSGT-delivered HGF also strikingly reduced various pathologic and molecular markers in vivo such as the level of collagens (type I and II), blood urea nitrogen (BUN), creatinine, and the expressions of ICAM-1, TIMP-1 and alpha-S1VIA which play a critical role in obstructive kidney functions. Therefore, CBA-PSGT should be further investigated because of its potential to alleviate UUO and kidney-related diseases using high affinity kidney targeting. (C) 2016 Elsevier Ltd. All rights reserved.</P>

Effect of Variety and Stage of Maturity on Nutritive Value of Whole Crop Rice, Yield, Botanical Fractions, Silage Fermentability and Chemical Composition

Islam, M.R.,Ishida, M.,Ando, S.,Nishida, T.,Yoshida, N.,Arakawa, M. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.2

The effect of eight varieties of grain and forage type whole crop rice (Oryza sativa L Japonica) each harvested at four stages of maturity were investigated for morphology and yield, proportion of botanical fractions, fermentatability and chemical composition in an $8{\times}4$ factorial experiment. All crops were sown in 1997 at Saitama Prefecture, Japan under identical condition and harvested on 10, 22, 34 and 45 days after flowering in 1998. Total DM yield of forage type varieties was similar to that of the highest yield of grain type varieties. However, while yield of forage type varieties was attributed to higher proportion of straw than head, the reverse was in the case of grain type varieties. Yield in line with the proportion of head increased (p<0.001), but in contrast proportion of straw decreased (p<0.001) with the increase in maturity. Silage fermentability of grain type varieties was better than forage type varieties. Fermentability improved with the increase (p<0.001) in maturity suggesting that the moisture content should be reduced to improve fermentation quality. Forage type varieties contained higher (p<0.001) ash, crude fat (EE), organic cell wall (OCW) and acid detergent fiber (ADF), but contained lower crude protein (CP), organic cell content (OCC), CP in OCC and nitrogen-free cell wall extract (NCWFE) than the grain type varieties. The ash, CP, EE, Oa (60% digestible OCW), Ob (40% digestible OCW), OCW, ADF and acid detergent lignin (ADL) decreased (p<0.001), but OCC and NCWFE increased (p<0.001) with the increase in maturity. It is concluded that stage of maturity not only increases yield and proportion of head, but also improved the fermentation quality and increases quality chemical composition (except CP) of whole crop rice. Forage type varieties may be as good as grain type varieties in terms of yield, but fermentation quality and chemical composition may not be as good as that of grain type varieties.

Remediation of PAHs contaminated soil by extraction using subcritical water

Islam, M.N.,Jo, Y.T.,Park, J.H. Korean Society of Industrial and Engineering Chemi 2012 Journal of industrial and engineering chemistry Vol.18 No.5

The remediation of polycyclic aromatic hydrocarbons (PAHs) contaminated soil has been investigated by extraction using continuous flowing subcritical water. Water temperature ranging from 100 to 300<SUP>o</SUP>C, extraction time ranging from 15 to 60min, and flow rate ranging from 0.5 to 2.0mL/min were investigated to determine their effect on the removal efficiencies of target PAHs. More than 95% extraction of the phenanthrene, fluoranthene, and pyrene from contaminated soil was observed at 300<SUP>o</SUP>C for 30min and 250<SUP>o</SUP>C for 60min at a constant pressure of 100bar. However, naphthalene was almost completely extracted only at a comparatively low temperature of 150<SUP>o</SUP>C and extraction time of up to 30min and a pressure of 100bar. The subcritical water flow rate of 0.5mL/min was recommendable in this study. The extraction efficiency of PAHs was extremely dependent on water temperature, since the dielectric constant (polarity) of water could be dramatically lowered by raising the water temperature. These results suggest that soils contaminated by persistent organic chemicals such as PAHs can be easily remediated by extraction using pure water under a high temperature without any modification.

EXACT SOLUTION FOR STEADY PAINT FILM FLOW OF A PSEUDO PLASTIC FLUID DOWN A VERTICAL WALL BY GRAVITY

M.K.ALAM,M.T.RAHIM,S.ISLAM,A.M.SIDDIQUI 한국산업응용수학회 2012 Journal of the Korean Society for Industrial and A Vol.16 No.3

Here in this paper, the steady paint film flow on a vertical wall of a non-Newtonian pseudo plastic fluid for drainage problem has been investigated. The exact solution of the non-linear problem is obtained for the velocity profile. Also the average velocity, volume flux, shear stress on the wall, force to hold the wall in position and normal stress difference have been derived. We retrieve Newtonian case, when material constant μ₁ and relaxation time λ₁ equal zero. The results for co-rotational Maxwell fluid is also obtained by taking material constant μ₁ = 0. The effect of the zero shear viscosity η?, the material constant μ₁, the relaxation time λ₁ and gravitational force on the velocity profile for drainage problem are discussed and plotted.