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Irfan Ahmad Rather,서병주,Vattiringal Jayadradhan Rejish Kumar,최욱한,최광호,임정희,박용하 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.1
Antifungal and biopreservation potentials ofLactobacillus plantarum YML007 isolated from Koreankimchi were analyzed. The biopreservative efficacy of thebacterium was analyzed using maize grains. Maize wasdivided into 3 groups and treated with a 5× concentratedcell-free supernatant of Lb. plantarum YML007 (T3R3),organic acids (T2R2), and a control group (T1R1) lackingtreatment. All groups were stored for 30 days. Maize wastested for moisture and crude fat contents, mold growth,and aflatoxin production. The moisture content, moldcount, and toxin production were higher in the controlmaize. The YML007 and acid treated maize remaineduninfected after 30 days. Animals fed with YML007treated maize showed more weight gain and less feedconsumption. YML007 can be used to preserve thenutritional value of stored grain and to ensure better qualityfeedstuffs that are necessary for improving animal healthand performance.
Irfan Ahmad Bhat,Rupam Sharma,Mohd Ashraf Rather,Pravesh Kumar Rathor,P. Gireesh‑Babu,Mukunda Goswami,J. K. Sundaray 한국유전학회 2017 Genes & Genomics Vol.39 No.9
The steroidogenic acute regulatory protein (StAR) plays a key role in transferring cholesterol across the inner mitochondrial membrane. In this study, the StAR gene was isolated from the gonads of Clarias batrachus. The gene has an open reading frame of 857 bp and encodes 285 amino acids with a predicted molecular weight of 32 kDa. The signalP analysis predicted that StAR would be a non-secreted protein that lacks a signal peptide. The subcellular localization demonstrated that the presence of the StAR protein was higher in mitochondria (41.8%), followed by the nuclear region (37.1%) and cytoplasm (11.1%). The StAR protein was found to interact highly with cyp11a1, followed by the cytochrome P450 family 11 proteins and the START5 domain. The homology modelling revealed that the protein has 4 helices and twisted U-shaped 10 beta sheets numbered from αA to αD and β1 to β10, respectively. Molecular modelling analysis showed that resveratrol and eurycomanone has high binding affinity with the StAR protein. The C. batrachus StAR transcript was found to be expressed exclusively in the gonads, kidney, and liver. These results overall lay a solid foundation for understanding the structure of StAR protein in fish. The identification of 3D structures and binding sites will help in designing a structure-based drug of StAR agonists for the treatment of impaired steroidogenesis.
Wee Yin Koh,Uthumporn Utra,Rosma Ahmad,Irfan A. Rather,박용하 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.5
A total of eight strains of lactic acid bacteria were isolated from water kefir grains and assessed for their in vitro a-glucosidase inhibitory activity. Lactobacillus mali K8 demonstrated significantly higher inhibition as compared to the other strains, thus was selected for in vitro probiotic potential characterization, antibiotic resistance, hemolytic activity and adaptation to pumpkin fruit puree. L. mali K8 demonstrated tolerance to pH 2.5 and resisted the damaging effects of bile salts, pepsin and pancreatin, comparable to that of Lactobacillus rhamnosus GG ATCC 53103 (reference strain). Lack of hemolytic activity and susceptibility to the five standard antibiotics indicated the safety of the K8 strain. This strain showed singular properties to be used as starters in the pumpkin fruit puree fermentation. These preliminary in vitro tests indicated the safety and functionality of the K8 strain and its potential as a probiotic candidate.
( Seo Byoung Joo ),( V. J. Rejish Kumar ),( Rather Irfan Ahmad ),( Byung Chun Kim ),( Wan Park ),( So Deuk Park ),( Se Eun Kim ),( Sang Dal Kim ),( Jeong Heui Lim ),( Yong Ha Park ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.1
The biological control efficacy of a greenhouse soil bacterial mixture of Lactobacillus farraginis, Bacillus cereus, and Bacillus thuringiensis strains with antinematode activity was evaluated against the root-knot nematode Meloidogyne incognita. Two control groups planted in soil drenched with sterile distilled water or treated with the broadspectrum carbamate pesticide carbofuran were used for comparison. The results suggest that the bacterial mixture is effective as a biocontrol agent against the root-knot nematode.