Recombinant canstatin inhibits angiopoietin‐1‐induced angiogenesis and lymphangiogenesis

Hwang‐,Bo, Jeon,Yoo, Ki Hyun,Park, Jong‐,Hwa,Jeong, Han‐,Sin,Chung, In Sik Wiley Subscription Services, Inc., A Wiley Company 2012 International journal of cancer: Journal internati Vol.131 No.2

<P><B>Abstract</B></P><P>We describe the effect of recombinant canstatin, the NC1 domain of the α2 chain of Type IV collagen, on suppression of angiogenesis and lymphangiogenesis both <I>in vitro</I> and <I>in vivo</I>. Recombinant canstatin produced from stably transformed <I>Drosophila</I> S2 cells reduced the expression of angiopoietin‐1 in hypoxia mimetic agent, CoCl<SUB>2</SUB>‐treated CT‐26 cells. Recombinant canstatin inhibited proliferation, tube formation and migration of human angiopoietin‐1 (rhAngpt‐1)‐treated human umbilical vein endothelial cells (HUVEC) and lymphatic endothelial cells (LEC). Recombinant canstatin suppressed the expression of Tie‐2 and vascular endothelial growth factor‐3 (VEGFR‐3) transcripts in rhAngpt‐1‐treated HUVEC and LEC, respectively. The inhibitory effect of recombinant canstatin on tumor growth was also investigated using a heterotopic CT‐26 colon carcinoma animal (BALB/c mice) model. Recombinant canstatin reduced the final volume and weight of tumors, and blood and lymphatic vessel densities of tumors, which were evaluated by CD‐31 and LYVE‐1 immunostaining. Immunohistochemical analysis showed that recombinant canstatin dramatically reduced the expression of angiopoietin‐1 in CT‐26 colon carcinoma‐induced tumor, but not the expression of VEGF‐C. Tie‐2 and VEGFR‐3 expressions were also reduced in recombinant canstatin‐treated tumors. These results indicate that recombinant canstatin has anti‐tumoral activities against CT‐26 colon carcinoma cells. Recombinant canstatin reduces the expression of angiopoietin‐1 in hypoxia‐induced CT‐26 cells and inhibits the angiogenic and lymphangiogenic signaling induced by angiopoietin‐1. Recombinant canstatin probably inhibits angiogenesis and lymphangiogenesis <I>via</I> suppression of the integrin‐dependent FAK signaling induced by angiopoietin‐1/Tie‐2 and/or VEGFR‐3.</P>

모델동물의 병태.생리 유발 유전자 기능 연구 : 당뇨모델동물의 췌장 β-세포를 이용한 비만발생에 관한 연구 Effect of development of diabetes by fatty acid in animal model

황대연,김용규,강태석,황진희,임채형,강현구,이주은,허영범,장인석,조정식,채갑용 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

체장 β세포에 지방이 축적되는 lopotoricity 현상은 인슬린 비의존성 당뇨 (Non InsuBin Dependent Diabetes Mellitus :NIDDM)의 발생과 상당히 밀접한 관계가 있으며, 췌장 β세포의 lipoapoptosis는 세포막형성에 관여하는 sphingomyelin의 전군체안 ceramide의 과생산에 의해 유도된다는 보고가 있다. 그러나, 지방산 종류에 따른 췌장 β세포에서의 세포사멸 (apoptosis)유발 기작에 대해서는 야직까지 보고된 바가 없파. 또한, 지방세포에서 생성되며, 지질대사, 음식섭취 및 체중조절에 관여하는 렙틴 (leptin)과 렙틴 수용체와의 관계 역시 체장 세포에서 명착하게 밝혀져 있지 않다. 따라서 본 연구에서는 6주령 53와 Zucker rat의 췌장으로부터 췌도를 분리하는 기술을 확립하고, 배양된 췌장 췌도 세포와 MS-1세포주에 불포차즌방산 (n-3 linolenic acid; n-6 :linoleic acid), 포화지방산 (stearic acid), conjugated linoleic acid (CLA)를 포함하는 4 가지의 지방산을 처리한 후 이들이 췌장 』세포의 사멸에 미치는 영향을 다양한 apoptosis 관련 유전자의 발현을 분석함으로써 확인하고자 하였다. 그 결과 SD rat으로부터 마리 당 평균 약 500개의 췌장 췌도를 분리하는데 성공하였으며, 4 종류의 지방산 중 포화지방산인 stearic acid를 처리하여 ONA fragmentation 증가를 관찰하였다. 또한 RT-PCR 방법을 이용하여 분리된 췌도 세포에사의 Bcl-2, Bax, iNOS가 지방산의 종류에 따라 서료 다른 발현양상을 나타낸다는 」라실을 관귿하였다. 이러한 떤구 결과는 지방산의 종류에 따라 췌장 췌도 세포에서의 세포사멸 유발 효과를 겋증하였으며, 이러한 연구결과는 복잡한 당뇨발병 원인 규명에 중요한 정보를 제공할 것으로 사료된다. Fat contents within the pancreatic islets are closely related to lipotoxicity which induces apoptosis, resulting in non insulin dependent mellituse(NIDDM). High level of fat may contribute to both insulin resistance and obesity in rodent and humans but the evidence is limited. To determine whether dietary fatty acids affect pancreatic cell function, the INS-1 cells and the pancreatic islets isolated from SD and Zucker obese(fa/fa) rats(6 wk, prediabetic stage) were cultured with stearic acid and CLA. As a result, DNA fragmentation laddering was substantially decreased in the INS-1 cells and the isolated pancreatic islets cultured with 2mM CLA compared with those with stearic aced. To investigate the mechanism by which CLA alleviates cell apoptosis as indicated by DNA fragmentation assay, we examined mRNA expressions of apoptosis-related proteins including Bax and Bcl-2 associated with cell death agonist and antagonist, respectively in the both INS-1 cells and islets cultured with 2 mM fatty acids. Bax mRNA expression was not markedly altered by either stearic acid or CLA, whereas Bcl-2 mRNA expression was noticeably enhanced by CLA compared with stearic acid. However, there were no changes in cell apoptosis and cell apoptotic-regulating gene products in the both INS-1 cells and isolated islets treated without or with 2 mM CLA. It is concluded that CLA may have a beneficial effect for maintenance of cell viability via increased Bcl-2 protein expression compared with steraic aced, which lead to delay or prevent, at least somewhat, the onset of NIDDM in the physiological status.

약물중독자의 회복 및 사회복귀에 관한 사례연구

한부식(Han Boo-Sik),황인옥(Hwang In-Ok) 경북대학교 사회과학연구원 2017 사회과학 담론과 정책 Vol.10 No.1

본 연구의 목적은 7년간 부산지역 NA모임의 스텝으로 봉사하면서 만난 약물중독자들의 사례연구를 통하여 약물중독자들의 회복과 사회복귀를 도모하는 데 있다. 연구대상자는 의도적 표집방법에 의하여 자발적으로 참여의사를 밝힌 총 7명을 선정하였고 심층면접을 통하여 자료수집을 하는 질적 사례연구방법을 적용하였다. 본 연구의 결과를 요약하면 다음과 같다. 첫째, 연구대상자들은 바닥을 치는 경험과 약물을 남용하면서 쌓인 많은 문제들로 인하여 약물의 남용을 후회하지만 스스로 단약 할 수 없었고, 결국 타인의 도움으로 병원, 치료공동체에 참여 하면서 약물 없는 생활을 시작하게 되었다. 둘째, 연구대상자들은 사회적 재활을 위하여 무절제하고 혼돈된 생활에서 벗어난 신앙생활, 규칙적인 생활, 새로운 취미활동, 대인관계와 가족관계의 회복을 위한 노력, 약물중독에 대한 공부 등을 통하여 일상생활에서 즐거움을 느끼며 살아갈 수 있게 되었다. 셋째, 사회적 재활을 통하여 취업에 성공한 연구대상자들은 일상생활에서 즐거움을 느끼며 살아갈 수 있게 되었지만, 사회복귀를 위한 직업재활의 부재로 인하여 사회복귀에 어려움을 겪고 있었다. 본 연구의 결과를 바탕으로 다음과 같은 제언을 하고자 한다. 첫째, 교정시설 내에서의 중독에 대한 교육과 NA모임을 통하여 출소 후 치료재활 현장으로 유도하는 방안이 필요하다. 둘째, 회복된 약물중독자들에 대한 직업재활 프로그램의 개발이 절실하다. 셋째, 약물중독자 가족이 약물중독을 이해할 수 있도록 하기 위한 교육 프로그램이 필요하다. 넷째, 회복된 약물중독자에 대한 교육을 통하여 다른 약물중독자들의 치료재활 교육에 투입할 필요가 있다. The purpose of this study is to promote recovery and rehabilitation for drug addicts through a case study of drug addicts who are participating in NA meetings that the researcher met in Busan NA meetings while volunteering for 7 years. A total of 7 study subjects, who agreed to participate in the study, were selected by using purposive sampling and a qualitative case study was applied for this study. And data was collected through an in-depth interview. Results from this study can be summarized as follows. First, As subjects experience the very bottom of their lives and face various difficulties and pains, they start to feel regret abusing drugs, but they were not able to stop abusing drugs by themselves and support and help from other people were required to live without using drugs as they participated in therapy groups. Second, The subjects were able to live and feel pleasure in daily life through social activities such as religious life, regular life, new hobbies, efforts to recover interpersonal and family relations, and study of drug addiction for social rehabilitation. Third, although they had been able to feel pleasure in daily life by social rehabilitation, they were having hard times returning to society due to lack of job rehabilitation for it. Based on these study results, there are some suggestions as follows. First, a plan is required to lead drug addicts to rehabilitation institutions after they are released from a jail by making them understand drug addiction through NA meetings and by education about treatment in a correctional facility. Second, the development of vocational rehabilitation programs for recovered drug addicts is required. Third, a training program to help drug addicts families understand drug addiction is necessary. Fourth, there is a need to invest in therapeutic rehabilitation education for other drug addicts through education about recovered drug addicts.

소형 개구면 결합 원형분극 마이크로스트립 배열안테나

황인진,정봉식 東亞大學校附設情報技術硏究所 2005 情報技術硏究所論文誌 Vol.12 No.2

In this paper the compactness of aperture-coupled array antenna with CP(circularly polarized) radiation for 2.4GHz wireless communication hand-set is designed. the compact operation of microstrip antenna is obtained by inserting suitable slits in the radiating patch, and CP radiation by perturbing the diagonal corners in the radiating patch, which generates the resonant mode of interest into two nearly degerate modes. Feed network of array antenna is made by using a two-layer and aperture-coupled structure. By numerical and experimental results, the patch size of compact CP micro strip antenna is reduced about 37% than conventional one without compact technique.

장내의 탄수화물과 지방 흡수 억제를 통한 체지방 및 비만 개선 효과에 관한 연구

정은희,윤승원,이홍석,윤유식,유경미,황인경 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.1

In a previous study, a dietary supplement was developed in our lab using natural herbal extracts against digest enzyme activity in GI tract for weight control. This natural herbal extracts could regulate absorption of glucose and lipid by the inhibition of digest enzyme activity. In this study, we screened the natural herbs that inhibit glucoamylase activity and developed an water extract of cinnamon. The cinnamon extract delayed and decreased the increment of carbohydrate degradation through the inhibition of glucoamylase activity in vitro. Fifty volunteers were subjected to the intake of the herbal extracts by taking twice a day for 60 days. As a result, the treated subjects lost 3kg of body weight and 3.5kg of body fat mass after the treatment. Furthermore, the body mass index and waist size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of cinnamon and natural herbal extract improves the obesity by the decrement of body weight and body fat mass.

十二經脈의 經脈名에 관한 考察

구성태,조명수,황재호,변기원,김경식,손인철 한국전통의학연구소 2002 한국전통의학지 Vol.12 No.1

In the present study, we aimed. to consider the name of twelve main Meridian in order to understand the concept of the Meridian system and to applicate acupuncture theory in the clinical field properly and fundamentally. We referred to several literatures on the matter and we found out that the name of present twelve Meridian was not settled until the period of Yellow Emperor's Classic. In the time of Yellow Emperor's Classic, the two theory of Internal Organ and Meridian system were combined and added new theory of 'WoonGi'. The general rule of combination is that the component of name of Meridian consist of 'Hand' or 'Foot', 'three Eum and Yang' and internal organ. The internal organ coupled with the root ki or middle ki of 'three Eum and Yang' and the 'Hand' or 'Foot' depend on the location of internal organ that belongs to meridian. The name of Twelve Meridian is a kind of system formed to analyze the two concepts organically and dynamically. One is material internal organ and the other is immaterial meridian system.

원자력 발전소용 이종재(Cu 합금/STS316L) 마찰용접의 최적화와 AE에 의한 실시간 평가에 관한 연구

유인종(IN-JONG YOO),권상우(SANG-WOO KWON),황성필(SUNG-PHIL HWANG),공유식(YU-SIK KONG),오세규(SAE-KYOO OH) 한국해양공학회 2001 韓國海洋工學會誌 Vol.15 No.2

In this paper, joints of Cu-1Cr-0.1Zr alloy to S1S316L were performed by friction welding method. Particularly, Cu-1Cr-0.1Zr alloy is attractive candidate as nuclear power plant material and exibit the best combination of high strength and good electrical and thermal conductivity of any copper alloy examined. The stainless steel is a structural material while copper alloy acts as a heat sink material for the surface heat flux in the first Wall. So, in this paper, not only the development of optimizing of friction welding with more reliability and more applicabililty but also the development of in-process real-time weld quality (such as strength and toughness) evaluation technique by acoustic emission for friction welding of such nuclear reactor component of Cu-1Cr-0.1Zr alloy to STS316L steel were performed.

골반골절 환자에서 출혈의 정도를 반영하는 분류

황태식,손대곤,김인병,장석준 大韓應急醫學會 1996 대한응급의학회지 Vol.7 No.1

The causes of death of pelvic bone fracture are hemorrhage, associated internal organ injuries, sepsis, and multi-organ failure, among which hemorrhage still remains as the leading cause of death. While previous classification for pelvic bone fracture was focused on orthopedic treatment, Pennel & Sutherland suggested a new classification according to the pelvis AP film took in the emergency room. Since this classification supposedly evaluates hemorrhage from pelvic bone fracture, we are reporting on the efficiency of this classification. Out of all the 150 patients admitted to Yong Dong Severance Hospital Emergency Room from 1992, Jan. through 1994, Dec. due to pelvic bone fracture, 18 people with incomplete medical record and X-ray film excluded from the study. The study was done Cohort method and t-test was used. According to the new classification, the unstable fracture had a much more bleeding amount regardless of the type, and type I stable fracture bled more than all the other stable fracture. These were statistically meaningful. Also, there was no differences in the type of fracture when ISS(Injury Severity Scale), GCS(Glasgow Coma Scale), and associated injury were compared. In conclusion, previous classification was done according to orthopedic treatment and this classification could be done only after pelvis X-ray series and pelvic bone CT was performed. Therefore much more time is consumed, more stress is put upon the patient and no comparisons can be made in performing emergency care. On the other hand, modified Pennel &-Sutherl`s classification can be simply classified in the emergency room by taking pelvis AP film only and the bleeding amount can be measured according to the type. Therefore, this classification can be helpful in performing emergency care.

Simulated resin root canal에서 엔진 구동형 Ni-Ti 파일로 근관성형 후 Buchanan plugger의 근관 내 삽입깊이에 대한 비교 연구

박연식,김동준,황윤찬,황인남,오원만 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.2

본 연구는 레진 블록에서 엔진구동형 Ni-Ti 기구를 이용하여 근관성형 후 Buchanan plugger의 삽입 깊이에 대한 비교를 통해 임상적 상황에서 Ni-Ti 파일로 근관 성형 후 Continuous wave of condensation technique을 이용한 근관 충전시 적절한 크기의 Buchanan plugger 선택에 지침을 제공하기 위해 시행되었다. 만곡근관과 직선근관을 가진 레진 블록에서 ProTaper와 ProFile을 이용하여 근관 성형을 하였다. 성형 후 근관에 Buchanan plugger 가 근관벽과 처음으로 접촉하는 부위까지 삽입한 후 그 상태에서 스태너를 이용하여 이미지를 얻었다. 이미지 분석 프로그램을 이용해 근첨에서 plugger 첨부까지의 거리를 얻었다. 각 기구에 대한 plugger 삽입깊이에 대한 유의성을 검정하기 위해 one-way ANOVA를 시행하였고, 사후검정을 위해 Scheffe's test를 시행하였다. 본 연구의 결과 만곡되고 좁읍 근관에서, ProTaper F2와 F3로 마무리 성형한 근관에서 F. FM 크기의 plugger를 사용할 경우 Continuous wave of condensation technique으로 적절한 근관충전이 가능함을 시사하였다. This study was conducted to evaluate the insertion depth of Buchanan plugger after shaping by various Ni-Ti rotary files. It was conducted to determine which size of plugger are appropriate, when root canals are shaped with Ni-Ti rotary files and obturated by Continuous wave of condensation technique. Two type of eighty simulated resin blocks were used : J-shaped and straight shaped canal. The simulated canals were instrumented by ProTaper and ProFile. Buchanan pluggers were inserted into the canal, and then the image was recorded to scanner. The distance from the apex of the canal to the plugger tip was measured by image analysis program. Data were analyzed by one-way ANOVA followed by Scheffe's test. The results were as follows 1. In straight canal finished up to ProTaper F2 and F3 file, F and FM pluggers were inserted more than 5 ㎜ short of working length. 2. In J-shaped canal finished up to ProTaper F2 file, F pluggers were inserted more than 5 ㎜ short of working length. Finished up to ProTaper F3 file, F and FM pluggers were inserted more than 5 ㎜ short of working length. 3. In straight and J-shaped canal finished up to ProFile . 06/#20 and ,06/#25, any of Buchanan plugger could not be inserted more than 5 ㎜ short of working lenghth. These results suggest that canals shaped by ProTaper could be obturated by Continuous wave of condensation technique with F and FM size Buchanan plugger.

액체상 방류물에 의한 주민선량 평가모델 입력변수의 민감도 분석

염정민,황원태,한문희,정운관,윤인식 조선대학교 에너지.자원신기술연구소 2003 에너지·자원신기술연구소 논문지 Vol.25 No.1

A model for off-site radiological dose evaluation due to liquid effluents generated from the operation of nuclear facilities, which are located in inland area, was developed in consideration of Korean agricultural and environmental conditions. In addition, sensitivity analysis was performed for the improvement of the model. Using the developed model, test runs for nuclear fuel fabrication facilities were conducted. Five kinds of exposure pathways(vegetation, animal products, drinking water, fresh-water-fish, shoreline deposits) and two kinds of radionuclides(234U, 234Th) were considered. Sensitivity analysis for input parameters of the model was conducted by applying a Monte Carlo method with Latin Hypercube Sampling (LHS) technique. As the results, the ingestion of irrigated agricultural products was the highest contributor among the considered exposure pathways. Major sensitive input parameters for the results were the ratio of the interception fraction to the yield of agricultural plants and the human consumption rate.