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Baek, In-Jeoung,Jung, Ki Youn,Yon, Jung-Min,Lee, Se-Ra,Lee, Beom Jun,Yun, Young Won,Nam, Sang-Yoon Springer 2011 In vitro cellular & developmental biology. Animal Vol.47 No.8
<P>Although it has been suggested that the transcription of phospholipid hydroperoxide glutathione peroxidase (PHGPx), an essential antioxidant selenoenzyme, may be affected by the estrogen state in mammals, the direct mechanism underlying the regulation of the PHGPx gene by estrogens in mammalian tissues remains to be clearly elucidated. In this study, we evaluated the expression of the PHGPx mRNA in cultured mouse fetuses (embryonic days 8.5-10.5) exposed to 17관-estradiol (E(2); 0.1, 1, 10, 100, and 1,000 ng/ml); estrogen receptor (ER) agonists [propyl pyrazole triol (PPT, an ER관-selective ligand, 1 관l/ml) and diarylpropionitrile (DPN, an ER관-selective ligand, 1 관l/ml)]; and/or ER antagonist [ICI 182,780 (ICI, 1 관l/ml)] using a whole embryo culture system. E(2)-alone treatment significantly stimulated the expressions of both ER관 and ER관 mRNAs in all the cultured fetuses (p < 0.05), although the ER관 mRNA levels were higher than ER관 mRNA. PHGPx mRNA expression was significantly increased in all the fetuses treated with E(2) (1-1,000 ng/ml), PPT, and DPN (p < 0.05). Furthermore, pretreatment with ICI completely blocked the E(2)-induced PHGPx mRNA expression in the fetuses. In addition, the mRNA levels of cytosolic GPx, the other intracellular antioxidant selenoenzyme, did not differ significantly from the controls by an exposure to those agents. These results suggest that the PHGPx gene is regulated via an estrogen and ER signal pathway in the cultured mouse fetus.</P>
In-Jeoung Baek,Jung-Min Yon,Se-Ra Lee,Wook-Jun Yoo,Sang-Soep Nahm,Yun-Bae Kim,Jai Seup Ro,Bang Yeon Hwang,Soon Sun Kim,Dae Hyun Cho,Beom-Jun Lee,Young Won Yun,Sang-Yoon Nam 한국실험동물학회 2006 Laboratory Animal Research Vol.22 No.1
Banha (Pinellia ternata) is a herbal medicine which is composed of alkaloid substances, homegentisic acid, 3,4-dihydroxybenzaldehyde and 3,4-diglycosilic benzaldehyde and is mainly prescribed in cough with dyspnea, gastric retention, distention and pain of the thorax and abdomen, lymph node tuberculosis, carbuncle, and furuncle. The use of raw Banha has been prohibited in pregnant woman because of it's strong toxicity. In this study, to investigate whether Banha affects the antioxidant system of male reproductive organs, we analyzed the mRNA expression patterns of glutathione peroxidase (GPx) family (type 1-4) in the reproductive organs of rats exposed to Banha. Banha extracts (0.05, 0.1, 0.2, 0.5, 1, and 2 g/㎏ body weight) were treated to 6 week-old rats for 4 weeks per oral. Total RNAs were extracted from testis, epididymis, prostate, and seminal vesicle and then RT-PCR was perfomed with the specific primers of each GPx enzyme. In epididymis and prostate, GPx1 mRNA were decreased by Banha treatment except for 2 g/㎏ treatment in prostate. However, GPx1 mRNA in seminal vesicle was increased by Banha treatment. GPx2 mRNA was increased in testis and seminal vesicle. GPx4 (PHGPx) mRNA was decreased in seminal vesicles by 0.1 and 1 g/㎏ treatment of Banha. These findings indicate that Banha may affect the antioxidant system of male reproductive organs, although further study is necessary.
Loss of Acot12 contributes to NAFLD independent of lipolysis of adipose tissue
Sujeong Park,Jinsoo Song,In-Jeoung Baek,Kyu Yun Jang,Chang Yeob Han,Dae Won Jun,Peter K. Kim,Brian Raught,Eun-Jung Jin 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
In this study, we hypothesized that deregulation in the maintenance of the pool of coenzyme A (CoA) may play a crucial role in thepathogenesis of nonalcoholic fatty liver disease (NAFLD). Specific deletion of Acot12 (Acot12−/−), the major acyl-CoA thioesterase,induced the accumulation of acetyl-CoA and resulted in the stimulation of de novo lipogenesis (DNL) and cholesterol biosynthesisin the liver. KEGG pathway analysis suggested PPARα signaling as the most significantly enriched pathway in Acot12−/− livers. Surprisingly, the exposure of Acot12−/− hepatocytes to fenofibrate significantly increased the accumulation of acetyl-CoA andresulted in the stimulation of cholesterol biosynthesis and DNL. Interaction analysis, including proximity-dependent biotin identification (BioID) analysis, suggested that ACOT12 may directly interact with vacuolar protein sorting-associated protein 33A (VPS33A) and play a role in vesicle-mediated cholesterol trafficking and the process of lysosomal degradation of cholesterol inhepatocytes. In summary, in this study, we found that ACOT12 deficiency is responsible for the pathogenesis of NAFLD through the accumulation of acetyl-CoA and the stimulation of DNL and cholesterol via activation of PPARα and inhibition of cholesterol trafficking.
Lin, Chunmei,Jeong, Ju-Yeon,Yon, Jung-Min,Park, Seul Gi,Gwon, Lee Wha,Lee, Jong-Geol,Baek, In-Jeoung,Nahm, Sang-Soep,Lee, Beom Jun,Yun, Young Won,Nam, Sang-Yoon The Korean Society of Veterinary Science 2017 大韓獸醫學會誌 Vol.57 No.2
Hyaluronic acid (HA) has been investigated for biomedical and pharmaceutical applications. This study was conducted to determine the distributions of HA nanoparticles (NPs; size 350-400 nm) and larger HA polymers in mice at intervals after application. $^{177}Lutetium$ (Lu)-labeled HA-NPs or HA polymers were intravenously injected (5 mg/kg) into male ICR mice, and radioactivity levels in blood and target organs were measured from 0.25 h to 28 days post-injection. In blood, the radioactivities of HA-NPs and HA polymer peaked at 0.5 h after injection but were remarkably decreased at 2 h; subsequently, they maintained a constant level until 6 days post-injection. HA-NPs and HA polymers were observed in the liver, spleen, lung, kidney, and heart (in ascending order) but were seldom observed in other organs. After 3 days, both the HA-NP and HA polymer levels showed similar steady decreases in lung, kidney, and heart. However, in liver and spleen, the HA-NP levels tended to decrease gradually after 1 day and both were very low after 14 days, whereas the HA polymer level accumulated for 28 days. The results indicate that HA-NPs, with their faster clearance pattern, may act as a better drug delivery system than HA polymers, especially in the liver and spleen.
Hye-Young Song,In-Jeoung Baek,Seung-Hee Ryu,Je-won Ryu,Eun-Young Park,Seung-Ho Heo 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7
Human immune system (HIS) mice have become valuable animal models for the study of human diseases, and peripheral blood mononuclear cell engrafted humanized mice (PBMCHu mice) mice provide a useful platform for cancer immunotherapy research. However, there have been limitations such as insufficient engraftment of human immune cells and development of graft versus host disease (GVHD). To overcome these limitation, we generated CD47; Rag2; IL2rg triple KO mice (TKO) using NOD-Rag2null IL2rgnull mice (DKO) and transplantation efficacy of human leukocytes and GVHD incidence were compared in PBMC-Hu mice. Examining flow cytometry results, more than 25% Human CD45+ cells were observed in both TKO groups from 3 weeks after PBMC administration, and it was observed more than 1 week later in DKO groups. Most of the engrafted cells were CD3+ T cells, and some CD19 B cells and CD66b+ granulocytes were also observed. The ratio of human leukocytes engraftment was greater in TKO mice, however, clinical symptoms of GVHD such as weight loss, jaundice, anemia and death of animals were more severe than in DKO mice. In histopathology, inflammatory cell aggregation, necrotic or apoptotic cells were observed in liver, lung and kidney, and semi-quantitative lesion scores were greater in TKO mice groups. Human CD45, CD3 and CD19 positive cells were also observed in immunohistochemistry. CD47 protein present on myeloid cells and inhibit phagocytosis through ligation of signal-regulatory protein alpha (SIRPα). In this study, we generate TKO mice to improve PBMC-Hu mice. Engraftment of human leukocytes was increased, but symptoms of GVHD were also more severe in TKO mice than DKO mice. Further study for overcoming development of GVHD and poor engraftment of leukocytes other than T cells is needed.