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Effects of CD26 in Parthenogenetically Activated Porcine Embryos
Mi-Ryung Park,Ji-Hyun Im,Hak-Jae Chung,Kyong-Woon Kim,Sung June Byun,Seongsoo Hwang,Gi-Sun Im 한국수정란이식학회 2016 한국동물생명공학회지 Vol.31 No.4
CD26, also known as Dipeptidyl peptidase IV (DPP-4), is a cell surface glycoprotein that belongs to the serine protease family and has wide spread organ distribution throughout the body. CD26 was previously characterized in immune cells but also has important metabolic functions which are not yet fully understood. Thus, we investigated the effect of CD26 in porcine parthenogenetic embryos. We attempted CD26 downregulation of porcine embryos by siRNA, and evaluated CD26 suppression of developmental competencies. Although the porcine embryos injected with CD26 siRNA were able to develop to the early stage, these embryos were decreased to form blastocysts. Our results indicated that CD26 is one of factors for the regulation of development of porcine embryos.
Kim, Im-Ryung,Cho, Ju-Hee,Choi, Eun-Kyung,Kwon, In-Gak,Sung, Young-Hee,Lee, Jeong-Eon,Nam, Seok-Jin,Yang, Jung-Hyun Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4
Objectives: Regardless of its negative impact on quality of life, little is known about the importance of alopecia from the patients' perspective. This study aimed to explore the whole experience of chemotherapy-induced alopecia among Korean breast cancer patients including perception, attitudes, preparedness, and changes after alopecia. Methods: Patients expected to experience or had experienced alopecia were recruited at a tertiary hospital in Seoul, Korea. Semi-structured in-depth interviews were performed in 21 patients. Recurrent issues were identified and placed into thematic categories. Results: All patients think that appearance is important and they pay attention to how they look like. They had negative perceptions about alopecia. Patients were not well prepared for alopecia, and experienced substantial physical, psychological and social distress. Lack of information and limited social support combined with negative images of cancer made it difficult for patients to overcome the trauma and deterred them from usual daily activities resulting in poor quality of life. Conclusions: Patients were not well prepared for alopecia and negative perceptions, lack of preparedness, and limited social support and resources increased alopecia-related distress. Educational programs for preparing patients to cope with alopecia distress and advocate activities to change people's negative perception about alopecia are needed to reduce the burden imposed by alopecia in cancer patients.
Effects of CD26 in Parthenogenetically Activated Porcine Embryos
Park, Mi-Ryung,Im, Ji-Hyun,Chung, Hak-Jae,Kim, Kyong-Woon,Byun, Sung June,Hwang, Seongsoo,Im, Gi-Sun The Korean Society of Embryo Transfer 2016 한국동물생명공학회지 Vol.31 No.4
CD26, also known as Dipeptidyl peptidase IV (DPP-4), is a cell surface glycoprotein that belongs to the serine protease family and has wide spread organ distribution throughout the body. CD26 was previously characterized in immune cells but also has important metabolic functions which are not yet fully understood. Thus, we investigated the effect of CD26 in porcine parthenogenetic embryos. We attempted CD26 downregulation of porcine embryos by siRNA, and evaluated CD26 suppression of developmental competencies. Although the porcine embryos injected with CD26 siRNA were able to develop to the early stage, these embryos were decreased to form blastocysts. Our results indicated that CD26 is one of factors for the regulation of development of porcine embryos.
임진옥,노숙령,이재관,옥현이 中央大學校 遺傳工學硏究所 1995 遺傳工學硏究論集 Vol.8 No.1
A study regarding serum-free media for the culture of rat primary hepatocyte was investigated. The effect of L-proline on the hepatocyte funtional activity was studied by measuring the hepatocyte differentiate functions. The addition of L-proline enhanced the amino acid metabolism by hepatocyte, especially, the rate of L-glutamine consumption was increased by 2-fold. The supplementation of L-proline replaced the requirement of serum in media. In serum-free media, L-proline supplementation supported the functional activity of hepatocyte for 7 weeks, and the albumin secretion rate was 6㎍/hr/2×10(6)cells. Kinetic measurement of DNA showed that the role of L-proline was not the stimulation of hepatocyte growth. Also, the requirement of L-proline in hepatocyte culture was specific for the sandwich configuration. Finally, in protein-free media without hormons or growth factors was maintained hepatic activity for 2 weeks. Summarized all together, L-proline is a indispensible factor in serum-free hepatocytes culture.
Developmental Competence and Targeting Efficiency of CRISPR/Cas9 Mediated hG-CSF
Mi-Ryung Park,Ji-Hyun Lim,Kyong-Woon Kim,Hak-Jae Chung,Poongyeon Lee,Taiyoung Hur,Gi-Sun Im 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10
The clustered regularly interspaced short plalindromic repeats(CRISPR)/CRISPR associated protein (Cas9) system can be applied to produce transgenic pigs. We applied CRISPR/Cas9 system to generate hG-CSF targeted pig parthenogenetic embryos. Using sigle guided RNA targeted to pig hG-CSF genes was injected into cytoplasm of in vitro matured oocyte before electrical activation. The CRISPR/Cas9 vector were diluted in Tris-EDTA buffer (TE buffer) and injected with different concentration of 0 (sham injection), 2.5 and 25 ng/ul. In results, regardless of the concentrations of vector, the cleavage and blastocyst rate were not significantly different among three groups. Since plasmid DNA was used for microinjection, we investigated whether DNA vectors were integrated into the genome. Genomic PCR of the coding sequence of Cas9 variants and hG-CSF was performed to detect genomic integrants. Each blastocysts were collected into a microtube, and then PCR was performed. Overall 32 embryos are not expressed targeted gene.