http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
RFLP Analysis of cag7 Gene of Helicobacter pylori
Hyung-Lyun Kang,Jeong-Uck Park,Mi-Young Choe,Kyung-Mi Kim,Do-Su Kim,Young-Chul Kwan,Seung-Gyu Park,Hyang-Ran Hwang,Jae-Young Song,Seung-Chul Baik,Woo-Kon Lee,Hee-Shang Youn,Myung-Je Cho,Kwang-Ho Rhee 대한미생물학회 2004 Journal of Bacteriology and Virology Vol.34 No.3
RFLP Analysis of cag7 Gene of Helicobacter pylori
Kang, Hyung-Lyun,Park, Jeong-Uck,Choe, Mi-Young,Kim, Kyung-Mi,Kim, Do-Su,Kwan, Young-Chul,Park, Seung-Gyu,Hwang, Hyang-Ran,Song, Jae-Young,Baik, Seung-Chul,Lee, Woo-Kon,Youn, Hee-Shang,Cho, Myung-Je The Korean Society for Microbiology 2004 Journal of Bacteriology and Virology Vol.34 No.3
The cag7 gene of Korean H. pylori strains was analyzed by RFLP to develop a discriminatory tool for genotyping clinical isolates. For this study, a total of 82 H. pylori strains were isolated from the patients; 27 strains from the patients with chronic gastritis, 26 from duodenal ulcer, and 29 from gastric cancer. Genomic DNA was isolated and subjected to PCR targeting entire ORF or the repeat regions I and II of cag7 gene. PCR products from entire ORF or repeat region I of cag7 gene were divided into two types. However, there was no difference in the length of PCR products from the repeat region II. By the PCR genotyping of the entire cag7 gene, genotypes A and B were established, which showed approximately 5,100 and 5,500 bp PCR products, respectively. The repeat region I showed approximately 600 or 1,000 bp DNA fragments by PCR. The length of cag7 gene was determined by the size variation in the repeat region I. In addition, RFLP analysis of the PCR products of cag7 gene showed 11 subtypes, based on the major bands. These findings illustrate that the genetic diversity of the repeat region I would serve a reliable target for the genotyping of the cag 7 gene.
Kang, Hyung-Lyun,Baik, Seung-Chul The Korean Society for Microbiology 2005 Journal of Bacteriology and Virology Vol.35 No.2
The whole cell extract of Helicobacter pylori strain 26695 was treated with the hemin-agarose resin and the bound fraction was analyzed by 2-Dimensional electrophoresis. The 2-D-PAGE-displayed spots were eluted and analyzed by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). Among the 120 spots processed, 94 protein spots were identified to represent 58 genes. Forty-five protein spots that represented thirty-four genes were newly identified in this study, including iron-containing proteins and hemin-containg proteins such as fumarate reductase, iron-sufur subunit(FrdB), ribonucleoside diphosphate reductase, beta subunit (NrdB), glutamyl-tRNA reductase (HemA), nikel-cobalt-cadnium resistance protein (NccB), and porphobilinogen deaminase (HemC).
A Small Cryptic Plasmid pZMO1 of Zymomonas mobilis ATCC10988
Kang, Hyung-Lyun,Kang, Hyen-Sam Korea Genome Organization 2003 Genomics & informatics Vol.1 No.1
The nucleotide sequence of pZMO1, a small cryptic plasmid of Zymomonas mobilis ATCC10988 was determined. Analysis of 1,680 bp of sequence revealed $69\%$ identity with Shigella sonnei plasmid, pKYM and $61\%$ identity with Nostoc sp. ss DNA replicating plasmid. Analysis of a deduced amino acid sequence of an orf of pZMO1 revealed $75\%$ identity and $90\%$ similarity with the repA gene of Synechocystis sp. plasmid pCA2.4. The upstream region of the repA gene of pZMO1 possesses six directed repeat sequences and two inverted repeat sequences at downstream of the IR consensus sequence of nick region of rolling circle replication (RCR) plasmid. A typical terminator hairpin structure was found at the downstream region of repA gene. Degradation of single-stranded plasmid DNA by S1 nuclease was detected by Southern hybridization. It suggests that pZMO1 replicates by a rolling circle mechanism in Z. mobilis ATCC10988 cells.