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Hyung Keung Oh,Won Kee Kim,Hyung Uk Jeong,Ah Rang Kang,Chang Wook Jin,Iksoo Kim 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05
The perilla leaf pyralid moth, Pyrausta panopealis Walker (Lepidoptera: Pyralidae), is a serious pest damaging to leaf perilla. In order to establish the life parameters of P. panopealis for eventual purpose of control, the developmental span of each stage were investigated under five temperature regimes (20℃ ~ 3 0℃). In addition, the larvicial efficacy of several on-the-market environment-friendly agricultural materials (EFAMs) was tested. The width of head capsule at each larval stage measured to be the mean of 0.21, 0.32, 0.47, 0.64, and 0.98 mm, respectively. The larval period of P. panopealis was longest at 20ºC as 27.0 days and shortened as temperature goes up to 30ºC as 11.3 days. Survivorship of the larval P. panopealis was the highest at 30ºC as 80%, whereas that of other temperatures ranged from 40% (20ºC) to 62.5% (27.5ºC), indicating that the P. panopealis appears to favor higher temperature. In addition to larval period, the duration of egg, prepupa, and pupa stages also shortened as temperature goes up, whereas the duration of adult stage increased as temperature goes up: from 4.1 days at 20 ºC to 6.1 days at 30ºC. After the perilla leaf pyralid moths were successfully stabilized in indoor environment the larvicidal efficacy of the ten EFAMs that were previously selected from the result of other moth species was tested aimed at 4th instar larvae for 48 hrs. Seven of the ten tested showed more than 90% of mortality within 12 hrs and reached nearly up to 100% within 24 hrs, but the remaining three showed less than ~70%.
Lian Jin, Hong,Pennant, William A.,Hyung Lee, Min,Su, Sung,Ah Kim, Hyun,Lu Liu, Meng,Soo Oh, Jin,Cho, Joon,Nyun Kim, Keung,Heum Yoon, Do,Ha, Yoon Lippincott Williams Wilkins, Inc. 2011 1528-1159) Vol.36 No.11
STUDY DESIGN.: An in vitro neural hypoxia model and rat spinal cord injury (SCI) model were used to assess the regulation of therapeutic vascular endothelial growth factor (VEGF) gene expression in mouse neural stem cells (mNSCs) by the EPO (erythropoietin) enhancer or RTP801 promoter. OBJECTIVE.: To increase VEGF gene expression in mNSCs under hypoxic conditions in SCI lesions but avoid unwanted overexpression of VEGF in normal sites, we developed a hypoxia-inducible gene expression system consisting of the EPO enhancer and RTP801 promoter fused to VEGF or the luciferase gene, then transfected into mNSCs. SUMMARY OF BACKGROUND DATA.: On the basis of the ischemic response in the injured area, poor cell survival at the transplantation site is a consistent problem with NSC transplantation after SCI. Although VEGF directly protects neurons and enhances neurite outgrowth, uncontrolled overexpression of VEGF in uninjured tissue may cause serious adverse effects. To effectively improve NSC survival in ischemic sites after transplantation, we evaluated mNSCs modified by a hypoxia-inducible VEGF gene expression system in an SCI model. METHODS.: Hypoxia-inducible luciferase or VEGF plasmids were constructed using the EPO enhancer or RTP801 promoter. The effect of these systems on targeted gene expression and cell viability was evaluated in mNSCs in both hypoxic in vitro injury and a rat SCI model in vivo. RESULTS.: The gene expression system containing the EPO enhancer or RTP801 promoter significantly increased the expression of the luciferase reporter gene and therapeutic VEGF gene under hypoxic conditions. The Epo-SV-VEGF plasmid transfection group had significantly fewer apoptotic cells in vitro. This system also augmented cell viability in the in vivo SCI model. CONCLUSION.: These results strongly suggest the potential utility of mNSCs modified by a hypoxia-inducible VEGF gene expression system in the development of effective stem cell transplantation protocols in SCI.
An, Sung Su,Jin, Hong Lian,Kim, Keung Nyun,Kim, Dong Seok,Cho, Joon,Liu, Meng-Lu,Oh, Jin Soo,Yoon, Do Heum,Lee, Min Hyung,Ha, Yoon Springer Verlag 2010 Child's nervous system Vol.26 No.3
<P>To avoid unwanted adverse effects of higher doses of single treatment of stem cells and gene therapy and increase the therapeutic efficacies, we hypothesized the combined therapy with stem cells and gene therapy. This study assessed the neuroprotective effects of combined gene therapy and stem cell treatment under ischemic hypoxia conditions using hypoxia-inducible vascular endothelial growth factor (VEGF) and bone marrow-derived mesenchymal stem cells (BMSC).</P>
Cha, Jin-Soon,Jeong, Min-Kyu,Kwon, Oh-Oun,Lee, Keung-Dong,Lee, Hyung-Soo Korean Chemical Society 1994 Bulletin of the Korean Chemical Society Vol.15 No.10
The approximate rate and stoichiometry of the reaction of excess diisobutylaluminum hydride-dimethyl sulfide complex($DIBAH-SMe_2$) with organic compounds containing representative functional group under standardized conditions (toluene, $0{\circ}C$) were examined in order to define the reducing characterstics of the reagent and to compare the reducing power with DIBAH itself. In general, the reducing action of the complex is similar to that of DIBAH. However, the reducing power of the complex is weaker than that of DIBAH. All of the active hydrogen compounds including alcohols, amines, and thiols evolve hydrogen slowly. Aldehydes and ketones are reduced readily and quantitatively to give the corresponding alcohols. However, $DIBAH-SMe_2$ reduces carboxylic acids at a faster rate than DIBAH alone to the corresponding alcohols with a partial evolution of hydrogen. Similarly, acid chlorides, esters, and epoxides are readily reduced to the corresponding alcohols, but the reduction rate is much slower than that of DIBAH alone. Both primary aliphatic and aromatic amides examined evolve 1 equiv of hydrogen rapidly and are reduced slowly to the amines. Tertiary amides readily utilize 2 equiv of hydride for reduction. Nitriles consume 1 equiv of hydride rapidly but further hydride uptake is quite slow. Nitro compounds, azobenzene, and azoxybenzene are reduced moderately. Cyclohexanone oxime liberates ca. 0.8 equiv of hydrogen rapidly and is reduced to the N-hydroxylamine stage. Phenyl isocyanate is rapidly reduced to the imine stage, but further hydride uptake is quite sluggish. Pyridine reacts at a moderate rate with an uptake of one hydride in 48 h, while pyridine N-oxide reacts rapidly with consumption of 2 equiv of hydride for reduction in 6h. Similarly, disulfides and sulfoxide are readily reduced, whereas sulfide, sulfone, and sulfonic acid are inert to this reagent under these reaction conditions.
Cha, Jin Soon,Jeong, Min Kyu,Kwon, Oh Oun,Lee, Keung Dong,Lee, Hyung Soo 대구효성가톨릭대학교 자연과학연구소 1994 基礎科學硏究論集 Vol.1994 No.1
The approximate rate and stoichiometry of the reaction of excess diisobutylaluminum hydbide-dimethyl sulfide complex(DIBAH-SMe_2)with organic compounds containing representative functional group under standardized conditions(tol-uene, 0℃)were examined in order to define the reducing characterstics of the reagent and to compare the reducing power with DIBAH itself. In general, the reducing action of the complex is similar to that of DIBAH. However, the reducing power of the complex is weaker than that of DIBAH. All of the active hydrogen compounds including alcohols, amines, and thiols evolve hydrogen slowly. Aldehydes and ketones are reduced readily and quantitatively to give the corresponding alcohols. However, DIBAH-SMe_2 reduces carboxylic acids at a faster rate than DIBAH alone to the corresponding alcohols with a partial evolution of hydrogen. Similarly, acid chlorides, esters, and epoxides are readily reduced to the corresponding alcohols, but the reduction rate is much slower than that of DIBAH alone. Both primary aliphatic and aromatic amides examined evolve 1 equiv of hydrogen rapidly and are reduced slowly to the amines. Tertiary amides readily utilize 2 equiv of hydride for reduction. Nitriles consume 1 equiv of hydride rapidly but further hydride uptake is quit slow. Nitro compounds, azobenzene, and azoxybenzene are reduced moder-ately. Cyclohexanone oxime liberates ca. 0.8 equiv of hydrogen rapidly and is reduced to the N-hydroxylamine stage. Phenyl isocyanate is rapidly reduced to the mnine stage, but further hydride uptake is quite sluggish. Pyridine reacts at a moderate rate with an yptake of one hydride in 48h, while pyridine N-oxide reacts rapidly with consumption of 2 equiv of hydride for reduction in 6th. Similarly, disulfides and sulfoxide are readily reduced, whereas sulfide, sulfone, and sulfonic acid are insert to this reagent under these reaction conditions.