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Choi, Young Deuk,Ham, Won Sik,Kim, Won Tae,Cho, Kang Su,Lee, Joo Hyoung,Cho, Soung Yong,Seo, Ju Wan,Jin, Ok Hyun Mary Ann Liebert 2009 Journal of endourology Vol.23 No.6
<P>PURPOSE: To evaluate the efficacy and safety of single-session OK-432 sclerotherapy for the treatment of renal cysts. MATERIALS AND METHODS: From October 2005 to November 2006, 48 patients (61 simple renal cysts) were included in the study. Indications were determined as flank discomfort (n = 37) or patient reassurance due to increasing size (n = 11). The simple renal cysts were aspirated under ultrasonography (US), at which point OK-432 was injected into the cyst. Follow-up was performed with US or computed tomography scan every 3 months until 1 year. Complete regression of the renal cyst or more than 70% reduction in size with no symptoms indicated a successful treatment. RESULTS: Among 61 renal cysts of 48 patients, the overall success rate was 98.4%. Complete regression occurred in 46 cysts (75.4%), and more than 90% reduction in size occurred in 6 cysts (9.8%). A size reduction of 80% to 90% and 70% to 80% occurred in five (8.2%) and three cysts (4.9%), respectively. A size reduction less than 70% occurred in only one cyst (1.6%). The success of cyst regression was correlated with cyst volume. Clinical symptoms resolved in 100% of patients with symptomatic cysts, and there was no enlargement of the aspirated cysts at the 1-year follow-up. After the procedure, there were only some minor complications, such as mild fever, flank pain, and leukocytosis, which subsided with the conservative treatment. CONCLUSIONS: Percutaneous OK-432 sclerotherapy is simple, safe, and effective, and it can be an alternative first-line therapy for simple renal cysts.</P>
흰쥐에서 가시오가피 발효물의 단회 및 반복투여 독성평가
조주현(Ju-Hyun Cho),박인재(In-Jae Park),백순옥(Soon-Ok Baik),최수영(Soo-Young Choi),최구희(Goo-Hee Choi) 한국식품과학회 2014 한국식품과학회지 Vol.46 No.2
본 연구에서는 사용된 가시오가피 영지버섯 발효물과 가시오가피 상황버섯 발효물에 대한 열수추출물을 최고 2 g/kg의 농도로 단회 투여한 SD-rats에 체중 변화, 임상증상 등에서 어떠한 독성도 관찰할 수 없었으며, 1 g/kg 이하의 농도로 4주간 반복 투여한 SD-rats에서도 체중변화, 임상증상, 장기 무게, 혈액학적 성상, 혈액생화학적 성상 등에서 어떠한 독성과 비정상적인 소견을 관찰할 수 없었다. 따라서, 본 연구의 결과로 볼 때 가시오가피 영지버섯 발효물 열수추출물(FM-5111)과 가시오가피 상황버섯 발효물 열수추출물(FM-5131)은 본 연구에서 사용한 농도 내에서의 안전성을 확인할 수 있었다. 황 함유 아미노산의 결핍은 여러 가지 간질환 등의 증상을 야기한다(27). 또한, Choi와 Ahn(28)은 오가피 부위별 50% (v/v) 메탄올 추출액의 전자공여능은 줄기 추출물에서 90.21%, 뿌리 추출물에서 85.71%로 높은 활성을 보고하여 오가피의 항산화능을 규명하였다. 따라서, 본 연구에 사용된 시료 또한 항산화능과 같은 기능성을 가질것으로 사료되며, 추가 연구로 아미노산 분석을 통해 황 함유 아미노산의 함량 변화를 분석하고 간 기능 개선 효능을 평가하여 안전성이 확보된 기능성 식품소재 개발 연구를 진행할 계획이다. In this study, the products of Acanthopanax senticosus fermentation were derived from the mycelia of 2 mushrooms, Ganoderma lucidum and Phellinus linteus, to determine their safety in Sprague-Dawley rats. Rats were orally administered the water extracts of A. senticosus fermentation products with G. lucidum (FM-5111) or P. linteus (FM-5131) at dose levels of 0.5, 1.0, or 2.0 g/kg for the single-dose toxicity test and 0.25, 0.5, or 1.0 g/kg for the repeated-dose toxicity test. There were no significant differences in body weight gain, feed intake, or water consumption between control and FM-5111- or FM-5131-treated rats. Hematological and blood biochemistry analysis revealed that none of the investigated parameters were affected by the A. senticosus fermentation products, and no remarkable lesions were observed upon histopathological analysis. We conclude that the A. senticosus fermentation products obtained from mushroom mycelia are safe for long-term administration and could be considered as multi-functional nutrients for the improvement of liver function and immunity.
CHOI, YUNG HYUN,IM, EUN-OK,SUH, HONGSUK,JIN, YOUNGEUP,LEE, WON HO,YOO, YOUNG HYUN,KIM, KYU-WON,KIM, NAM DEUK 부산대학교 유전공학연구소 2001 분자생물학 연구보 Vol.17 No.-
The therapeutic efficacies of bile acids, such as ursodeoxycholic acid (UDCA) and chenodeoxycholic acid (CDCA), have been widely demonstrated in various liver diseases, suggesting that they might protect hepatocytes against common mechanisms of liver damage. Although they have been shown to prevent apoptotic cell death in certain cell lines, we have previously reported that a novel derivative (HS-1030) of UDCA significantly inhibited cell growth and induced apoptosis in cancer cells. To develop more effective agents, we synthesized several derivatives, named HS-1183, HS-1199 and HS-1200, based on the structure of UDCA and CDCA, and CDCA had no significant range tested, both HS-1199 and HS-1200 completely inhibited the cell proliferation, and HS-1183 showed only a weak inhibitory activity. Furthermore, chromatin condensation, DNA ladder formation and proteolytic cleavage of poly(ADP-ribose)
Inhibition of cell growth and telomerase activity of breast cancer cells in vitro by retinoic acids
CHOI, SANG-HO,KANG, HYUN-KYUNG,IM, EUN-OK,KIM, YOUNG JOON,BAE, YOUNG TAE,CHOI, YUNG HYUN,LEE, KYUNG HEE,CHUNG, HAE-YOUNG,CHANG, HEE-KYUNG,KIM, NAM DEUK 부산대학교 유전공학연구소 2000 분자생물학 연구보 Vol.16 No.-
The effects of retinoic acid (RA) and its analogs, all-trans RA, 9-cis RA and 13-cis RA, were investigated in human breast cancer MCF-7 cells and immortalized breast epithelial cell line MCF-10A. RA inhibited the telomerase activity of MCF-7 cells in a wide range of concentrations. RA at 10 μM also inhibited the growth of MCF-7 cells in a time-dependent manner. However, no significant growth inhibition was found between untreated control and RA-treated MCF-10A cells. Moreover, a marked inhibition of telomerase activity by RA was detected early in MCF-7 cells (after 24 h of RA treatment), which was preceded by a reduction of hTERT mRNA expression (after 12 h of RA treatment). However, MCF-10A cells showed a reduction of telomerase activity and down-regulation of hTERT after 4 days fo RA treatment. Simultaneous changes in hTERT mRNA expression and telomerase activity were found for MCF-10A cells. The expressions of hTR and hTEP1 telomerase component genes were not changed after RA treatment. These results indicate that the anti-breast cancer activity of RA could be mediated by its ability to down-regulate the expression of hTERT telomerase gene.
Choi, Eun-Kyung,Park, Hyun-Jung,Sul, Ok-Joo,Rajasekaran, Monisha,Yu, Rina,Choi, Hye-Seon American Physiological Society 2015 AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND M Vol.308 No.8
<P>We hypothesized that carbon monoxide (CO) might suppress chronic inflammation, which led to metabolic disturbances. Ovariectomy (OVX) was performed in mice to mimic chronic inflammation secondary to loss of ovarian function. OVX increased fat mass and the infiltration of highly inflammatory CD11c cells into adipose tissue (AT), resulting in a disturbance of glucose metabolism. Treatment of CO attenuated these; CO decreased recruitment of CD11c-expressing cells in AT and reduced expression of CD11c in bone marrow-derived macrophages, protecting them from M1 polarization. Upregulated cGMP and decreased reactive oxygen species were responsible for the inhibitory activity of CO on CD11c expression; knockdown of soluble guanylate cyclase or heme oxygenase-1 using small interfering RNAs reduced this inhibition substantially. Improved OVX-induced insulin resistance (IR) by CO was highly associated with its activity to attenuate AT inflammation. Our results suggest a therapeutic value of CO to treat postmenopausal IR by reducing AT inflammation.</P>
<i>Salimicrobium jeotgali</i> sp. nov., isolated from salted, fermented seafood
Choi, Eun Jin,Jin, Hyun Mi,Kim, Kyung Hyun,Jeon, Che Ok International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.11
<P>A Gram-staining-positive, aerobic, motile bacterium, designated strain MJ3<SUP>T</SUP>, was isolated from <I>myeolchi-jeot</I> (anchovy jeotgal), a traditional fermented seafood in South Korea. Cells were non-endospore-forming cocci showing catalase- and oxidase-positive reactions. Growth of strain MJ3<SUP>T</SUP> was observed at 15–45 °C (optimum, 30 °C), at pH 6.0–9.0 (optimum, pH 7.0–8.0) and in the presence of 1–24 % (w/v) NaCl (optimum, 10 % NaCl). Phylogenetic inference based on 16S rRNA gene sequences showed that strain MJ3<SUP>T</SUP> formed a tight phyletic lineage with members of the genus <I>Salimicrobium</I>. Strain MJ3<SUP>T</SUP> was related most closely to <I>Salimicrobium salexigens</I> 29CMI<SUP>T</SUP>, <I>Salimicrobium album</I> DSM 20748<SUP>T</SUP>, <I>Salimicrobium flavidum</I> ISL-25<SUP>T</SUP>, <I>Salimicrobium luteum</I> BY-5<SUP>T</SUP> and <I>Salimicrobium halophilum</I> DSM 4771<SUP>T</SUP>, with similarities of 98.8 %, 98.7 %, 98.6 %, 98.4 % and 98.3 %, respectively. However, the DNA–DNA relatedness values between strain MJ3<SUP>T</SUP> (KF732837) and <I>S. salexigens</I> DSM 22782<SUP>T</SUP>, <I>S. album</I> DSM 20748<SUP>T</SUP>, <I>S. flavidum</I> DSM 23127<SUP>T</SUP>, <I>S. luteum</I> KCTC 3989<SUP>T</SUP> and <I>S. halophilum</I> JCM 12305<SUP>T</SUP> were 60±5.4 %, 58.5±6.5 %, 43.6±5.5 %, 37.2±5.8 % and 16.7±0.2 %, respectively. Chemotaxonomic data (sole isoprenoid quinone, MK-7; major cell-wall type, <I>meso</I>-diaminopimelic acid; major cellular fatty acids, anteiso-C<SUB>15 : 0</SUB>, anteiso-C<SUB>17 : 0</SUB> and iso-C<SUB>15 : 0</SUB>; major polar lipids, phosphatidylglycerol and diphosphatidylglycerol; DNA G+C content, 46.3 mol%) also supported the affiliation of strain MJ3<SUP>T</SUP> with the genus <I>Salimicrobium</I>. Therefore, strain MJ3<SUP>T</SUP> represents a novel species of the genus <I>Salimicrobium</I>, for which the name <I>Salimicrobium</I> <I>jeotgali</I> sp. nov. is proposed. The type strain is MJ3<SUP>T</SUP> ( = KACC 16972<SUP>T</SUP> = JCM 19758<SUP>T</SUP>).</P>