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Preparation and Characterization of Polypyrrole-Coated Silicon Nanoparticles
La, Hyun Shil,Jeun, Joon Pyo,Choi, Jae Hak,Kang, Phil Hyun,Lee, Youn Sik,Nho, Young Chang Trans Tech Publications, Ltd. 2007 Diffusion and defect data. SSP. [Pt. B], Solid sta Vol.119 No.-
<P>Silicon (Si) nanoparticles were stabilized by sodium dodecyl sulfate and poly(Nvinylpyrrolidone) in water, and coated with polypyrrole (PPy) via an in-situ polymerization of pyrrole with FeCl3. TEM images revealed that the Si nanoparticles were successfully coated with PPy (average thickness, ~2 nm). The Li/PPy-coated Si electrode exhibited improved discharge capacities, when compared to that of a reported Li/pure Si electrode. PPy-coatings on the Si nanoparticles acted as efficient conducting agents and prevented an extraction of the cracked Si fragments into the electrolyte to some extent.</P>
Increased lipid productivity of Acutodesmus dimorphus using optimized pulsed electric field
La, Hyun-Joon,Choi, Gang-Guk,Cho, Chuhyun,Seo, Seong-Hyun,Srivastava, Ankita,Jo, Beom-Ho,Lee, Jae-Yon,Jin, Yun-Sik,Oh, Hee-Mock Springer-Verlag 2016 Journal of applied phycology Vol.28 No.2
<P>An alternative stress-inducing method, a low-energy pulsed electric field (LE-PEF), was used to improve the lipid productivity of microalgae cultures. A large shift in the Nile-Red-stained peaks toward a higher intensity in fluorescent-activated cell sorting, suggestive of an increased neutral lipid content, was observed when 10 kV LE-PEF pulses were applied to 800 mL batch cultures of Acutodesmus dimorphus. The optimal LE-PEF on-off cycle treatment for A. dimorphus was 2 s on-60 s off for 15 min with 6 cycles per day. Under these optimal LE-PEF treatment conditions, A. dimorphus showed an overall 28.8 % increase in lipid productivity. Therefore, based on these results, LE-PEF can be regarded as a promising tool to avoid the lipid content and growth rate trade-off and improve the lipid productivity of microalgae in various cultivation systems.</P>
Seo, Seong-Hyun,Ha, Ji-San,Yoo, Chan,Srivastava, Ankita,Ahn, Chi-Yong,Cho, Dae-Hyun,La, Hyun-Joon,Han, Myung-Soo,Oh, Hee-Mock Elsevier 2017 Bioresource technology Vol.244 No.1
<P><B>Abstract</B></P> <P>The optimal culture conditions are critical factors for high microalgal biomass and lipid productivity. To optimize the photoautotrophic culture conditions, combination of the pH (regulated by CO<SUB>2</SUB> supply), dilution rate, and light intensity was systematically investigated for <I>Ettlia</I> sp. YC001 cultivation in a chemostat during 143days. The biomass productivity increased with the increase in dilution rate and light intensity, but decreased with increasing pH. The average lipid content was 19.8% and statistically non-variable among the tested conditions. The highest biomass and lipid productivities were 1.48gL<SUP>−1</SUP> d<SUP>−1</SUP> and 291.4mgL<SUP>−1</SUP> d<SUP>−1</SUP> with a pH of 6.5, dilution rate of 0.78d<SUP>−1</SUP>, and light intensity of 1500μmolphotonsm<SUP>−2</SUP> s<SUP>−1</SUP>. With a sufficient supply of CO<SUB>2</SUB> and nutrients, the light intensity was the main determinant of the photosynthetic rate. Therefore, the surface-to-volume ratio of a photobioreactor should enable efficient light distribution to enhance microalgal growth.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Biomass productivity of <I>Ettlia</I> sp. was maximized using CO<SUB>2</SUB>-controlled chemostats. </LI> <LI> Highest biomass productivity of 1.48gL<SUP>−1</SUP> d<SUP>−1</SUP> at 1500μmolphotonsm<SUP>−2</SUP> s<SUP>−1</SUP>. </LI> <LI> Lipid content was around 19.8% regardless of pH and light intensity variation. </LI> <LI> Light intensity was the key factor for high biomass productivity. </LI> </UL> </P>
Development of orthotopic patient-derived tumor xenograft models in endometrial cancer
( Hyun-jin Choi ),( Eun Jin Heo ),( Young Jae Cho ),( Ji Eun Hong ),( Hye-kyung Jeon ),( Doo-yi Oh ),( Yoon-la Choi ),( Sangyong Song ),( Jung-joo Choi ),( Yoo-young Lee ),( Chel Hun Choi ),( Tae-joon 대한산부인과학회 2016 대한산부인과학회 학술대회 Vol.102 No.-
목적: To evaluate orthotopic xenografting of endometrial cancer(EM Ca) in mice for development of novel translational research platform in endometrial cancer. 방법: Fresh surgical specimens were taken from patients with EM Ca after surgery at Department of Obstetrics and Gynecology, Samsung Medical Center, Seoul, Korea between December, 2011 to February, 2016. We successfully established PDXs by orthotopic and subrenal capsule implantation of patient’s EM Ca tissues into female BALB/C-nude mice. The subrenal capsule implatation method were that pieces of EM Ca specimens from patients were meticulosely grafted in the subrenal capsule. Mean while, estrogen preconditioning was done for orthotopic implatation by given a daily s.c injection of 0.1μg estradial for three days. After then, pieces of EM Ca specimens from patients were meticulosly grafted in the enlarged mice’s uterus. The H&E staining, short tandem repeat, array comparative genomic hybridization, human and mouse albumin test and cancer panel were conducted to verifythe histopathologic and genetic similarity between the PDXs and primary patient’s tissues. 결과: Orthotopic PDX engraftment rate were sucessful in 100% (3/3 cases). While, Successful rate of subrenal capsule implantation method was 55.6% (10/18 cases). In orthotopic PDXs, median duration of first generation of PDX develoment from implantation of patient’s tissues was 5.43month (range: 0.83-6.63 month). And, in subrenal capsule implantation PDXs, median duration of first generation was 5.05month (range : 4.17-9.33 month). Both methods were proved to preserve histopathologic and genetic silimarity between PDXs and the primary patient’s tissues. 결론: The PDXs for endometiral cancer with histopathologic and genetic stability can be efficiently developed by an orthotopic implantation method rather than a subrenal capsule implantation method. This method can a promising platform for conducting translational research and future precision medicine in endomerial cancer.
Preparation of Ethyl-Cellulose Nanofibers via An Electrospinning
Jeun, Joon Pyo,Lim, Youn Mook,Choi, Jae Hak,La, Hyun Shil,Kang, Phil Hyun,Nho, Young Chang Trans Tech Publications, Ltd. 2007 Diffusion and defect data. SSP. [Pt. B], Solid sta Vol.119 No.-
<P>Ethyl-cellulose (EC) nanofibers were fabricated by an electrospinning of an EC solution with a 6-12 wt% concentration. Fiber morphology was observed under a scanning electron microscope and the effects of instrument parameters including the solution concentration, flow rate and electric voltage were investigated.</P>
<i>Caulobacter</i> <i>profunda</i> sp. nov., isolated from deep freshwater sediment
Jin, Long,La, Hyun-Joon,Lee, Hyung-Gwan,Lee, Jay Jung,Lee, Sanghyup,Ahn, Chi-Yong,Oh, Hee-Mock International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.3
<P>The Gram-stain-negative, aerobic, non-spore-forming, motile, with a single polar flagellum, or non-motile (stalked) and rod-shaped bacteria, DS48-5-2<SUP>T</SUP> and DS48-6-3, were isolated from a sediment sample collected from a depth of 48 m taken from Daechung Reservoir, Republic of Korea. Comparative 16S rRNA gene sequence studies showed that the two isolates had clear affiliation with <I>Alphaproteobacteria</I> and the closest relatedness to <I>Caulobacter mirabilis</I> FWC 38<SUP>T</SUP>, <I>Caulobacter fusiformis</I> ATCC 15257<SUP>T</SUP> and <I>Caulobacter daechungensis</I> H-E3-2<SUP>T</SUP> showing 98.5 %, 97.3 % and 97.3 % 16S rRNA gene sequence similarity, respectively, and 96.1–96.7 % similarity to all other species of the genus <I>Caulobacter</I>. The two isolates shared 100 % 16S rRNA gene sequence similarity. The predominant ubiquinone was Q-10. The major fatty acids were summed feature 8 (C<SUB>18 : 1</SUB>ω6<I>c</I> and/or C<SUB>18 : 1</SUB>ω7<I>c</I>), C<SUB>16 : 0</SUB>, C<SUB>18 : 0</SUB>ω7<I>c</I> 11-methyl and summed feature 3 (C<SUB>16 : 1</SUB>ω6<I>c</I> and/or C<SUB>16 : 1</SUB>ω7<I>c</I>). The G+C contents of the genomic DNA of strains DS48-5-2<SUP>T</SUP> and DS48-6-3 were 66.7 mol% and 66.2 mol%, respectively. DNA–DNA hybridization values of strains DS48-5-2<SUP>T</SUP> and DS48-6-3 with <I>C. mirabilis</I> FWC 38<SUP>T</SUP>, <I>C. fusiformis</I> ATCC 15257<SUP>T</SUP> and <I>C. daechungensis</I> H-E3-2<SUP>T</SUP> were 19.3 %–24.4 %. Thus, based on the evidence from polyphasic studies, it is proposed that strains DS48-5-2<SUP>T</SUP> and DS48-6-3 are representatives of a novel species in the genus <I>Caulobacter</I>, for which the name <I>Caulobacter</I> <I>profunda</I> sp. nov. is proposed. The type strain is DS48-5-2<SUP>T</SUP> ( = KCTC 32480<SUP>T</SUP> = JCM 19440<SUP>T</SUP>).</P>
BIOLOGY OF A Cronartium BLISTER RUST ON Pinus densiflora
Chang Keun Yi,Hyun Joong Kim,Yong Joon La 한국산림과학회 1985 한국산림과학회지 Vol.71 No.1
Among the genus Cronartium, C. ribicola and C. quercuum have been recorded as the only species in Korea. In 1978, we discovered a Japanese red pine (Pious densiflora S. et Z.) infected with a stem rust in a pine plantation in Taebaeg-shi, Kangweon-do. Again in spring of 1985, a Japanese red pine tree with typical blister symptom was discovered in a P, densiflora plantation in Youngduk-gun, Gyeongsangbuk-do. Accordingly studies were carried out on its morphology and etiology to identify this rust. Aeciospores on the Japanese red pine and urediniospores on its alternate hosts, i.e., Paeonia spp., were indistinguishable from the aeciospores and urediniospores of the white pine blister rust in morphology when observed by the optical microscope and the scanning electron microscope. When the aeciospores of C. flaccidum were inoculated on some suspected alternate hosts in a growth cabinet, a large number of uredia appeared on undersurface of the leaves of Paeonia spp. and Pedicularis resupinata after 20 and 30 days, respectively following inoculation. In germination test, more than one percent of the aeciospotes of both C. ribicola and C. flaccidum remained viable for 90 days when they were stored in dark at 5℃. But regardless of the light condition,aeciospores of both species failed to germinate when stored at 20℃ for 30 days. The germination rate of the urediniospores of C. flaccidum from the Paeonia albiflora was about 1.5% when they were maintained at 21℃ with 100% relative humidity for 24 hours. C. ribicola having white pine as host and C. flaccidum having Japanese red pine as host were found to infect Pedicularis resupinata also.